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Citations for GE Life Sciences :
6551 - 6600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The GST-ScLas1 mutants were further purified on a Heparin HP column (GE Healthcare) and ScGrc3 mutants were purified on a HitTrap Q HP column (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... and ScGrc3 mutants were purified on a HitTrap Q HP column (GE Healthcare). All proteins were further purified by size-exclusion chromatography (Superdex 200 Increase 10/300 ...
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Biophysics 2023Quote: ... Eluted protein was digested with Ulp1 protease at 4°C for 2 h and then further purified on a Heparin HP column (GE Healthcare), eluting with buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatant was then circulated over 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) that was inoculated with 100mM CoCl2 for ten minutes ...
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bioRxiv - Cell Biology 2023Quote: ... and the chemiluminescent system AI680 (GE Healthcare) as described (Miyata & Nishida ...
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bioRxiv - Cell Biology 2023Quote: ... Signals were developed with horseradish peroxidase-conjugated secondary antibodies (GE Healthcare Bio-sciences) or peroxidase-conjugated primary antibodies using Western Lightning Plus-ECL (PerkinElmer ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then applied to a 10 mL packed GST resin (GE Healthcare, Waukesha, WI) equilibrated in Wash Buffer [PBS with 250 mM NaCl and 1 mM DTT] ...
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bioRxiv - Bioengineering 2023Quote: ... Released mesh probes were rinsed with deionized (DI) water three times and transferred to sterile 1× PBS solution (HyClone, GE Healthcare Life Sciences) before surface modification or implantation.
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bioRxiv - Biophysics 2023Quote: ... and the filtered supernatant loaded onto a HisTrap HP affinity column (GE Healthcare), and the protein eluted using an imidazole gradient ranging from 25-500 mM ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing fusion protein were pooled and exchanged into a SUMO protease cleavage buffer (20 mM Tris-HCl at pH 8.0, 0.15 M NaCl) using a HiPrep 26/10 column (GE Healthcare Life Sciences). The cleavage reaction was initiated by adding 6×His-tagged SUMO protease to a final concentration of ∼8 μg/mL to the protein solution ...
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bioRxiv - Biophysics 2023Quote: ... Concentrated protein was then subjected to single step buffer exchange with Dialysis Buffer (20mM Tris-Cl, pH 8.0, 50mM NaCl) with the help of aPD10 desalting column (GE Healthcare, Sephadex G-25 M matrix) under gravity flow ...
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bioRxiv - Biophysics 2023Quote: ... protein samples were purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with a buffer containing 0.03% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... and mixed with NHS-activated Sepharose 4 Fast Flow resin (GE Healthcare) conjugated with GFP nanobody ...
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bioRxiv - Biophysics 2023Quote: ... and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare) with Tris-NaCl buffer (50 mM Tris ...
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bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted protein was bound to Ni2+ resin (Ni Sepharose 6 Fast Flow, GE Healthcare) in 6M guanidine HCl ...
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bioRxiv - Biophysics 2023Quote: ... The 35S-methionine and 35S-cysteine incorporation into the core- and complex-glycosylated CFTR variants were visualized by fluorography and quantified using phosphorimage analysis on a Typhoon imaging platform (GE Healthcare). CFTR radioactivity was quantified after background subtraction ...
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bioRxiv - Biophysics 2023Quote: ... followed by gel filtration chromatography on a Superdex 200 16/60 column (GE Healthcare), in 50 mM Tris pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... The eluate was subjected to size-exclusion chromatography on a Superdex 200i 300/10 (GE Healthcare Life Sciences) in a buffer containing 50 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Biophysics 2023Quote: ... Anti-GFP nanobodies were conjugated with CNBr Sepharose beads (GE Healthcare, #17-0430-02). The resin was added to the cleared supernatant at a ratio of 1 ml of resin per 10 g of cell powder and incubated at 4 °C for 3 h with gentle shaking ...
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Biophysics 2023Quote: ... and loaded onto a Superdex 200 Hiload 16/600 (GE Healthcare) in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... and Superdex 200 10/300 GL column (GE Healthcare). Elf1core was purified in the same manner as wild-type protein ...
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bioRxiv - Biophysics 2023Quote: ... followed by Hi-Trap Heparin (GE Healthcare) and Mono Q anion exchange chromatography columns (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... and buffer exchanged into a final buffer (20 mM Tris, pH 7.4, 500 mM NaCl) using a HiPrep Desalting column (GE Healthcare). The purified protein was flash-frozen in liquid nitrogen and stored at -20α C.
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bioRxiv - Biophysics 2023Quote: ... and Mono Q anion exchange chromatography columns (GE Healthcare). Pol II was purified by incubating 10-subunit Pol II with 3-fold of Rpb4/7 followed by gel filtration ...
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bioRxiv - Biophysics 2023Quote: ... and Superdex 200 10/300 GL columns (GE Healthcare). Rad26 mutants were purified in the same manner as wild-type proteins ...
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bioRxiv - Biophysics 2023Quote: ... The resulting suspensions were centrifuged (12.000 rpm for 35 min at 4α C) and the supernatants were purified through a HisTrap HP column (GE Healthcare) previously equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... Pol II (with a protein A tag in the Rpb3 subunit) was purified by an IgG affinity column (GE Healthcare), followed by Hi-Trap Heparin (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare), and Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... Hi-Trap Heparin HP (GE Healthcare), and Superdex 200 10/300 GL columns (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... The peak associated with pure ACBP was concentrated and further purified by SEC using a Superdex 75 (HR 10/30) column coupled to an Äkta purifier system (GE Healthcare) using Buffer A ...
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bioRxiv - Biophysics 2023Quote: ... and loaded into a Mono Q 5/50 GL (GE Healthcare Life Sciences, Chicago, IL, USA) coupled to an Äkta purifier system (GE Healthcare) ...
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bioRxiv - Bioengineering 2023Quote: ... The DNA was blotted onto a nylon membrane (Amersham Hybond-N+; GE Healthcare, Chicago, IL, USA) and hybridized with DIG-labeled probe using DIG Easy Hyb (Roche Diagnostics ...
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bioRxiv - Bioengineering 2023Quote: ... Amersham Hyper film (GE Healthcare) was used for capturing the chemiluminescent signals.
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation using Ficoll-Paque Plus (GE Healthcare). Plasmacytoid dendritic cells and monocytes were isolated using anti-BDCA4 or anti-CD14 magnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... The sample was loaded onto a HiTrap Q FF column (GE Healthcare Life Sciences) on an ÄKTA Start Protein Purification System (GE Healthcare Life Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... on an ÄKTA Start Protein Purification System (GE Healthcare Life Sciences). α-Syn was eluted at approximately 300 mM NaCl with a salt gradient from 0 mM to 1000 mM ...
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bioRxiv - Neuroscience 2023Quote: ... and then transferred to a PVDF membrane (0.45 µm pore size, Amersham Hybond, GE Healthcare) via electro-blotting ...
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bioRxiv - Neuroscience 2023Quote: ... The CT images were obtained using the Discovery CT750 HD (GE Healthcare) for four subjects and the Aquilion Precision (Toshiba Medical Systems ...
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bioRxiv - Cell Biology 2023Quote: ... The protein eluate was captured by HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) and eluted by IMAC-B buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity capture was performed using FPLC and a HiTrap IMAC column (17-5248-01, GE Healthcare, Chicago, IL) equilibrated with IMAC-A buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... then transferred on nitrocellulose membrane (0.45 μm; GE Healthcare Lifesciences, 10600062) for 90 min at 400 mA ...
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bioRxiv - Cell Biology 2023Quote: ... All proteins were further purified by gel filtration on preparative Superdex HiLoad 26/600 or analytical (Superdex 200 10/300) columns (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... and indicated prey proteins at a 5X molar excess to tepsin (mATG8 and/or AP-4 β4 appendage) were incubated with glutathione sepharose resin (GE Healthcare) for 2 hours at 4°C in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2023Quote: ... Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham, GE Healthcare Life Sciences). The membrane was blocked in 5% skimmed milk (HIMEDIA ...
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bioRxiv - Cell Biology 2023Quote: ... soluble proteins were applied to the size exclusion gel filtration column (Superdex 200 Increase 10/300 GL, GE Healthcare), using an Akta P-920 Pump FPLC system (Amersham ...
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bioRxiv - Cell Biology 2023Quote: ... Dynein complexes were captured from Sf9 cell lysates using IgG Sepharose 6 FastFlow beads (GE Healthcare), labeled with SNAP-Cell-TMR-Star ...