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Citations for GE Life Sciences :
6001 - 6050 of 6873 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue was then digested with 1 mL of enzyme-free dissociation agent HyClone™ (GE Healthcare Life Sciences) for 5 min at 37°C ...
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bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed in 1 x TBST and incubated in the appropriate secondary antibodies (anti-Rabbit, GE Healthcare, NA934V ...
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bioRxiv - Cancer Biology 2024Quote: ... a condition using non-targeting siRNA (ON-TARGETplus Non-targeting Control siRNA #1, from Dharmacon, GE Healthcare, USA) was used at the same concentration as the siRNA targeting the genes of interest ...
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bioRxiv - Biophysics 2024Quote: ... (1994) but used FPLC steps of heparin-affinity using a HiTrap Heparin-HP 1 ml column (GE Healthcare), followed by anion exchange chromatography using a MonoQ 5/50GL (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... RRM2 (>5 mg mL-1) was injected onto a 24 mL Superdex 75 size exclusion column (GE Healthcare) at a flow rate of 1 mL min-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... DprA was purified from the clarified lysate by two-step chromatography using HisTrap HP 1 mL (GE Healthcare) and HiTrap SP XL 1 mL columns (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Systems Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by Ficoll-Paque (GE Healthcare, 17-1440-02) density gradient centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Images were analyzed automatically by the IN Cell Developer Toolbox software (cat. 25-8098-26, GE Healthcare Bio-Sciences, Marlborough, MA). Nuclei of living and dead cells were identified by using the corresponding fluorescent signal of Hoescht33342 ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2022Quote: Hela and Hela-RNAseH1 cells downregulated for the indicated genes were grown in the presence of 10 μM bromodeoxyuridine (BrdU, GE Healthcare) for 24 hours ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... After two centrifugation steps at 3000 x g to remove cell debris the AAV was isolated from the supernatant using heparin columns (GE Healthcare). After loading the column with the virus particles and washing with buffers containing 20 mM Tris and increasing concentrations of NaCl (100 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... and harvested from cell culture medium followed by purification procedure using Ni-NTA column and Superdex 200 gel filtration column (GE Healthcare) in tandem ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with standard erythrocyte lysis buffer on ice and separated from myelin and debris by Percoll gradient (GE Healthcare) centrifugation ...
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bioRxiv - Systems Biology 2019Quote: Time-lapse fluorescent microscopy for U2OS-Dual cells was performed at the biomolecular screening facility (BSF, EPFL) using an InCell Analyzer 2200 (GE healthcare). Experiments were performed at different temperature (34°C ...
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bioRxiv - Immunology 2019Quote: ... The brain tissues were quickly dissected and single cell suspensions were prepared and separated using a 37%/70% Percoll gradient (#GE17-0891-01, GE Healthcare). Myelin was removed from the top layer and mononuclear cells were isolated from the interface ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... After cell lysis by sonication cell debris was removed by centrifugation and the lysate was passed through a His-Trap column (GE Healthcare). Proteins were eluted with a gradient of buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 4 ml PBS and subject to density gradient centrifugation with Ficoll-Paque PLUS (17144002, GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... YL½ antibody (IgG, expressed from hybridoma cells and purified by ion exchange chromatography) was coupled to CNBr-activated sepharose beads (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... connected to a ÄKTA Pure apparatus equipped with a UV 280 nm flow cell (all from GE Healthcare, Chicago, IL, USA). EV fractions were collected based on UV absorbance ...
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bioRxiv - Microbiology 2020Quote: His-tagged SARS VHH-72 or MERS VHH-55 was immobilized to a single flow cell of an NTA sensorchip at a level of ∼400 response units (RUs) per cycle using a Biacore X100 (GE Healthcare). The chip was doubly regenerated using 0.35 M EDTA and 0.1 M NaOH followed by 0.5 mM NiCl2 ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...
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bioRxiv - Cancer Biology 2020Quote: ... were diluted with PBS and layered with 35 mL of diluted cell suspension over 15 mL of Ficoll-Paque (GE healthcare) in 50 mL conical tube ...
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bioRxiv - Cell Biology 2021Quote: ... were collected by crushing of bones (tibias, femurs, hips, humeri and spine) and subjecting the cells to density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare) at 400g for 25 minutes with brakes turned off ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Biophysics 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin and gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from age- and sex-matched mice by centrifugation over Ficoll-Paque Plus (GE Healthcare). Splenocytes were obtained by crushing the tissue through a 70μm nylon filter (Falcon) ...
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bioRxiv - Bioengineering 2022Quote: Human adult peripheral blood mononuclear cells (PBMCs) were purified by density centrifugation using Ficoll-Paque (density = 1.077 g/mL; 600g, 30 minutes; GE Healthcare; USA). Informed written consent was given by donors to give approval for the use of waste material for research purposes ...
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Microbiology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were separated from blood samples by density gradient centrifugation using Percoll (GE Healthcare, Chicago, IL, USA). Whole blood was lysed with ACK lysing buffer to separate white blood cells ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from the blood by lymphocyte isolation (Ficoll-Paque PREMIUM; GE Healthcare, Piscataway, NJ, USA) and plated in duplicate wells ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... As mentioned above GFP positive HIV infected cells were enumerated by fluorescence microscopy using the high content CYTELL imaging platform (GE Healthcare). An average of 25,000 cells were analysed per condition by acquiring multiple fields of view ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: Purified His-tagged Pentamer was immobilized to a single flow cell of a NiNTA sensor in a Biacore X100 (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Immunology 2020Quote: ... blood samples were collected during hospitalization and follow-up visits and separated into plasma and peripheral blood mononuclear cells (PBMCs) by Ficoll-Hypaque gradient (GE Healthcare) centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... human adult peripheral blood was harvested from healthy donors after informed consent and immune cell isolation using Ficoll-Paque gradient (GE Healthcare). Buffy coat was harvested for PBMCs while granulocytes were isolated from the gradient pellet ...
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bioRxiv - Bioengineering 2020Quote: ... were isolated from Trima Accel leukoreduction system (LRS, Memorial Blood Center) chambers using ammonium chloride-based red blood cell lysis followed by Ficoll-Paque gradient (GE Healthcare). PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin followed by gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...