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Citations for GE Life Sciences :
5501 - 5550 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Plant Biology 2019Quote: ... The lysed cells were centrifuged and the supernatant filtered and applied to a single 1ml His-Trap FF (GE Healthcare Life Sciences, WA) column that was dedicated to each ARF ...
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bioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll- Hypaque density gradient centrifugation (GE Healthcare Bio-Sciences AB, Uppsala, Sweden) according to manufacturer-provided instructions ...
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bioRxiv - Microbiology 2020Quote: ... The tissue solution was passed through two cell sieves (100µm then 70µm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... the H57-scFV was concentrated with a stirred 10 kDa ultrafiltration cell (PBGC04310, MERCK and purified by means of gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) using an ÄKTA pure chromatography system (GE Healthcare Life Sciences) ...
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Systems Biology 2021Quote: ... Then a microscope slide cover was gently placed onto of the agarose pad and cells were imaged using a DeltaVision inverted epi-fluorescence microscope (Applied Precision, Issaquah, WA). Images were captured using a CoolSnap HD charge-coupled device (CCD ...
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bioRxiv - Developmental Biology 2020Quote: ... embryos were injected with mRNAs and DNAs at the four-cell stage using a PicoSpritzer setup in 1/3x MR with 2.5% Ficoll PM 400 (GE Healthcare, #17-0300-50), and were transferred after injection into 1/3x MR containing 50μg/ml Gentamycin ...
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bioRxiv - Neuroscience 2020Quote: ... and hyperphosphorylated 2N4R tau produced in Sf9 cells to LRP1 or the VSP10 domain of SORL1 were assessed using a Biacore 3000 optical biosensor system (GE healthcare Life Sciences) essentially as described (M ...
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bioRxiv - Cancer Biology 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood from healthy volunteers by Ficoll-Paque density centrifugation (Ficoll-Paque PLUS, GE Healthcare Life Sciences) and enriched for CD8+ T cells with the MagniSort™ Human CD8+ T cell Enrichment Kit (#8804-6812-74 ...
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Cytokine and phenotypic cell profiles in human cutaneous leishmaniasis caused by Leishmania donovanibioRxiv - Microbiology 2022Quote: Peripheral Blood Mononuclear Cells (PBMC) were isolated from EDTA blood by density gradient centrifugation using 30% of Ficoll-Paque (GE Life Sciences, country). Cells were incubated at 37°C in 5% CO2 and monocytes were separated by adherence after 24 hours and cultured in complete RPMI1640(Gibco ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated by standard ficoll density gradient centrifugation from 70mL heparinized blood immediately after blood withdrawal (GE Healthcare, Uppsala, Sweden). For the first cohort ...
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bioRxiv - Plant Biology 2019Quote: ... The cells were lysed using an MSE Soniprep 150 (Wolf Laboratories, York, UK) and the cell-free lysate was applied to a 5 mL HisTrap HP column (GE Healthcare, Amersham, UK) and PL1 was eluted using a 5–500 mM imidazole gradient ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I and FANCI was incubated with Strep-Tactin Sepharose High Performance resin (GE Healthcare Life Sciences) for 60 min ...
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bioRxiv - Microbiology 2021Quote: ... IMR-32 (ATCC, CCL-127) and BHK-21 cells (ATCC, CCL-10) were maintained in minimal essential medium (MEM) (GE Healthcare Life Sciences) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Pathology 2020Quote: ... The tissue solution was passed through two cell sieves (100μm then 70μm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 min on dissociated tissue and on whole blood ...
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bioRxiv - Molecular Biology 2021Quote: ... the ORF region was PCR-amplified from HeLa cells cDNA and subcloned into the pcDNA3-Flag and pGEX6p2 vectors (GE Healthcare Life Sciences). In these plasmids ...
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bioRxiv - Microbiology 2019Quote: Slides for cell surface curvature enrichment analysis were imaged on a DeltaVision OMX V4 BLAZE 3D microscope (GE Healthcare Life Sciences, Chicago, IL) equipped with Photometrics Evolve 512 emCCD cameras and a UPlanApo 100x/1.42 oil objective with oil matched for the sample refractive index ...
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bioRxiv - Microbiology 2021Quote: Whole cells lysate (50 µg) was separated on 12% SDS-PAGE and then transferred onto a PVDF membrane (GE Healthcare, Piscataway, NJ, USA). Membrane were blocked in 5% (w/v ...
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bioRxiv - Cancer Biology 2021Quote: ... GST-fusions were purified from extracts of BL21 (DE3)/pLysS cells using Glutathione Sepharose 4B beads as recommended by the manufacturer (GE Healthcare Life Sciences). The kinase reaction was conducted at 30°C for 20 minutes and stopped by adding 2X Laemmli loading buffer ...
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bioRxiv - Cell Biology 2021Quote: ... hMBs and C2C12 cells were differentiated in a differentiation induction medium (DIM) consisting of DMEM with 2% horse serum (HyClone; GE Healthcare, UT, USA) and PS (Nihashi et al. ...
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bioRxiv - Immunology 2020Quote: ... Fractions that yielded proliferation of TN T cells were pooled and subjected to cation exchange chromatography on a Mono S 5/200 GL column (GE Healthcare, Piscataway NJ). The active fractions were again pooled and subjected to buffer exchange (Amicon ultra 3k cutoff ...
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bioRxiv - Microbiology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated and depleted of CD8+ T cells in a single step by density centrifugation using FicollPaque PLUS (GE Healthcare Life Sciences), SepMate tubes (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: Peripheral blood was collected in EDTA tubes and mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll®-Paque Premium (GE Healthcare Biosciences). CD14+HLA-DRneg/low/CD14+HLA-DRhigh monocytes cells were FACS-sorted from PBMC ...
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bioRxiv - Cell Biology 2020Quote: ... Localization of mCherry- and GFP-tagged fusion proteins was performed by fluorescence microscopy of live yeast cells using a Visitron spinning disk CSU-W1 (Visitron Systems, Puchheim Germany) or a DeltaVision deconvolution microscope (Applied Precision, Issaquah, WA). The Visitron spinning disk CSU-W1 consisted of a Nikon Ti-E inverted microscope ...
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bioRxiv - Molecular Biology 2019Quote: Epithelial skin cells were collected from male and female volunteers using a Whatman® FTA® Sterile Omni Swab (GE Healthcare, Chicago, IL). Participants were asked to swab the sides of their nose and behind their ears for approximately thirty seconds using swabs in order to maximize skin cell yield ...
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bioRxiv - Microbiology 2020Quote: Approximately 5 ml of blood were obtained from one patient and the peripheral blood mononuclear cells were isolated by centrifugation using a Ficoll-Paque PLUS (GE Healthcare, Uppsala, Sweden). These cells were then fused with SPYMEG cells at a ratio of 10:1 ...
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bioRxiv - Immunology 2022Quote: ... liver and enzyme treated lung were prepared by meshing organs through 70 μm nylon cell strainers in sRPMI-1640 with 8% FBS (HyClone Laboratories, GE Healthcare, Logan, Utah). After red blood cells lysis with NH4Cl (liver ...
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bioRxiv - Cell Biology 2022Quote: Super-resolution imaging of fixed cell samples with 3D-SIM was performed on a DeltaVision OMX V3 system (Applied Precision Imaging/GE Healthcare) equipped with a 100x/1.40 NA PlanApo oil immersion objective (Olympus) ...
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2022Quote: ... was created by mechanical disruption (tonsils were minced and pressed through a 70-micron cell screen) followed by Ficoll-Paque PLUS density gradient centrifugation (GE Healthcare Life Sciences). CD19-positive cells were removed (StemCell ...
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bioRxiv - Immunology 2022Quote: ... and purified from pre-conditioned cell media supernatant using Ni-NTA resin and size exclusion chromatography with a S200 column on an AKTAPure FPLC (GE Healthcare; Chicago, IL).
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bioRxiv - Cancer Biology 2024Quote: GST and GST-Rheb were expressed in BL21 cells and purified by affinity chromatography using Glutathione Sepharose High Performance beads (GE Healthcare Life Sciences) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: The freshly prepared buffy coats collected from healthy donors were used to purify Peripheral blood mononuclear cells (PBMCs) (Sylvan N. Goldman Oklahoma Blood Institute, Oklahoma City, OK) using Ficoll Paque (GE Healthcare, Piscataway, NJ)-based density centrifugation method ...
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bioRxiv - Biophysics 2023Quote: ... Cell suspensions were filtered through a 70 µm cell strainer, centrifuged (5 min, 500 g) and pellets were resuspended in DMEM containing 30% stock isotonic Percoll (SIP, GE Healthcare, Diegem, Belgium). Hereafter ...
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bioRxiv - Immunology 2023Quote: ... ILCIC-D06 and ILCIC-D12 datasets were isolated using Ficoll density gradient centrifugation (LymphoprepTM, Stem Cell Technologies; Ficoll-Plus, GE Healthcare Biosciences AB). PBMCs belonging to the ILCIC-D01 and ILCIC-D03 datasets were isolated using Vacutainer® CPT tube (BD Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... WT and C111S constructs were extracted from harvested cells by homogenization and soluble fractions loaded on a 5 ml HisTrap FF Crude column (GE Healthcare Life Sciences) equilibrated in binding buffer [20 mM Tris pH8.0 ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated from heparinized blood samples using standard Ficoll-Paque Plus gradient separation (GE Healthcare, Chicago, IL, USA). Cells were stored in 10% dimethyl sulfoxide in fetal bovine serum (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Peripheral blood mononuclear cells (PBMC) from healthy donors were isolated by ficoll density separation according to the manufacturer’s protocol (GE Healthcare, Chicago, Illinois, USA). Informed consent was given in accordance with the Declaration of Helsinki ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding ORF regions were PCR-amplified from HeLa cells cDNA with specific primer sets (Supplementary Table S2) and subcloned into the pcDNA3-Myc or pGEX6p2 vectors (GE Healthcare Life Sciences). Overlap extension PCR was performed to induce the mutation in the ULM of pcDNA3-Myc-SAP30BP (pGEX6p2-SAP30BP/ULMmt).
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bioRxiv - Immunology 2023Quote: NHP CD20 CAR-T cells were transduced and expanded as previously described:23 Peripheral blood mononuclear cells (PBMC) were isolated from adult NHP peripheral blood by Ficoll-PaquePLUS (GE Healthcare Bio-Sciences) or SepMate-50 (Stemcell) ...
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bioRxiv - Biochemistry 2024Quote: ... H2AX (total) (#2595S; Cell Signaling technology]. The membranes were extensively washed with 1% TBST followed by incubation with anti-isotype secondary antibody (GE Healthcare) conjugated with horseradish peroxidase in 5% skimmed milk at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... LLC2 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (GE Healthcare Life Sciences, HyClone Laboratories) and 1% penicillin and streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The filtered soluble cell solution was passed through an equilibrated Nickel-charged IMAC using a 5 mL HisTrap HP (GE Healthcare Life Sciences) in Lysis Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... A549-luc cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (GE Healthcare Life Sciences, HyClone Laboratories) and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... samples were red cell and granulocyte depleted by density gradient separation using Ficoll-Paque PLUS (GE Healthcare Life Sciences, cat. no. 17-5442-03) and CD34 enrichment was carried out on freshly isolated mononuclear cells (MNC ...
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bioRxiv - Cell Biology 2021Quote: Human peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll density gradient using Ficoll Plaque Premium (Sigma GE Healthcare, #17-544-02) or BD Vacutainer CPT Tubes (BD ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...