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Citations for GE Life Sciences :
5301 - 5350 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Human peripheral blood mononuclear cells were isolated from fresh whole blood by Ficoll-Paque (GE Healthcare, Little Chalfont, United Kingdom). CD4+CD45RA+ naïve T cells were isolated using negative enrichment kits (Stemcell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood through Ficoll density centrifugation (Ficoll-Plaque PLUS, GE Healthcare, USA), aliquoted and stored in liquid nitrogen ...
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bioRxiv - Immunology 2020Quote: ... mononuclear cells were isolated from buffy coats blood using Ficoll-Paque™ PLUS (γ=1.007, GE Healthcare, Chicago, IL, USA). Next ...
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bioRxiv - Cell Biology 2021Quote: Procyclic (PCF) cell line Tb427 29.13 Trypanosoma brucei brucei (86) was grown in SDM-79 medium (GE Healthcare, G3344-3005) pH7.4 supplemented with 2 mg/mL hemin (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation using Ficoll-Paque Premium (#17-5442-02; GE Healthcare). PBMCs were washed 3x with PBS ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated in two hours by using Ficoll Paque Plus (17-1440-02, GE Healthcare), deposited in RNAlater (Am7021#RNAlater Soln ...
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bioRxiv - Microbiology 2022Quote: Cell densities were determined based on the optical density at 600 nm (OD600) using an Ultraspec 2100 spectrophotometer (GE Healthcare) or a Tecan Infinite M1000 microplate reader ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Germany) and exchanged to the desired cell culture medium using Sephadex G-25 PD10 desalting columns (GE Healthcare Bio-Science). The final concentration and integrity of eGFP-WNT-3A in the conditioned medium were determined using both ELISA (GFP ELISA® kit ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed with cold phenol red-free DMEM with 10% FBS and 20 mM HEPES (GE Healthcare Life Sciences) to remove unbound viruses and incubated at 37 °C to initiate virus-cell fusion ...
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bioRxiv - Microbiology 2023Quote: ... the cell supernatant was collected and soluble IgG was purified using affinity chromatography (GammaBind Plus Sepharose, GE HealthCare, Chicago, IL). Purified IgG was buffer-exchanged in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Live imaging of GFP or RFP immunofluorescence in COs and NICOs was obtained using IN Cell Analyzer 2200 (GE Healthcare) at 4x or 10x magnification ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation on lymphocyte separation medium (LSM 1077, GE Healthcare) as previously described191 ...
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bioRxiv - Immunology 2024Quote: ... Liver infiltrating cells were obtained after 25 min centrifugation (600 g without brake) in a 35% and 67.5% bilayer Percoll (GE Healthcare) gradient ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation on Ficoll-Paque Plus (GE Healthcare, Boston, MA, USA). CD3+ T cells from PBMCs were separated using CD3 microbeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01 ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood samples by separation on a Ficoll-Hypaque gradient (GE Healthcare). For detailed splenocyte isolation protocol ...
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bioRxiv - Molecular Biology 2023Quote: The supernatant or cell extract was then filtered through a 0.45 µm pore filter and loaded on a HisTrap HP column (GE Healthcare) with immobilized Ni2+ beads equilibrated with HisBufferA (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells were isolated from screened buffy coats by density centrifugation with Ficoll-Plaque Plus (GE Healthcare) followed by magnetic-assisted cell sorting ...
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bioRxiv - Biophysics 2023Quote: YidC and YidCΔC were purified from pTrc99a-YidC (Welte et al., 2012) and pTrc99a-YidCΔC expressing BL21 cells using a Ni-NTA FF crude column (GE Healthcare) on an ÄKTA chromatography system ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then imaged at 4X using the InCell 2200 or 6500 automated fluorescence microscope system (GE Healthcare Life Sciences) and quantitative analysis was performed with Cell Profiler Software(182,183) ...
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bioRxiv - Immunology 2023Quote: ... from which the debris and the dead cells were removed by density gradient centrifugation with a 46% Percoll (GE Healthcare) gradient ...
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bioRxiv - Molecular Biology 2023Quote: Images of mouse spermatocyte spreads testicular cell squashes were acquired using a computer-assisted fluorescence microscope system (DeltaVision; Applied Precision) with a 60ξ NA 1.4 oil immersion objective ...
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bioRxiv - Immunology 2023Quote: ... Lymphocytes were isolated from the cell suspension using a 37 % and 70 % percoll gradient (GE Healthcare Bio-Sciences AB, Sweden) diluted with HBSS (with or without phenol red ...
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bioRxiv - Biochemistry 2023Quote: RNA was isolated from plated cells or snap-frozen kidneys using the RNAspin Mini kit (GE Healthcare, #25-0500-72) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, GE healthcare) and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Bioengineering 2024Quote: ... the soluble fraction of the cell lysate was passed through a 0.45-micron filter and then loaded onto a HisTrap HP 5 ml (GE Healthcare) column equilibrated with buffer A (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Systems Biology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood of healthy donors by Ficoll-Paque (GE Healthcare, 17-1440-02) density gradient centrifugation ...
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bioRxiv - Cell Biology 2020Quote: Images were analyzed automatically by the IN Cell Developer Toolbox software (cat. 25-8098-26, GE Healthcare Bio-Sciences, Marlborough, MA). Nuclei of living and dead cells were identified by using the corresponding fluorescent signal of Hoescht33342 ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2022Quote: Hela and Hela-RNAseH1 cells downregulated for the indicated genes were grown in the presence of 10 μM bromodeoxyuridine (BrdU, GE Healthcare) for 24 hours ...
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... After two centrifugation steps at 3000 x g to remove cell debris the AAV was isolated from the supernatant using heparin columns (GE Healthcare). After loading the column with the virus particles and washing with buffers containing 20 mM Tris and increasing concentrations of NaCl (100 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... and harvested from cell culture medium followed by purification procedure using Ni-NTA column and Superdex 200 gel filtration column (GE Healthcare) in tandem ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated with standard erythrocyte lysis buffer on ice and separated from myelin and debris by Percoll gradient (GE Healthcare) centrifugation ...
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bioRxiv - Systems Biology 2019Quote: Time-lapse fluorescent microscopy for U2OS-Dual cells was performed at the biomolecular screening facility (BSF, EPFL) using an InCell Analyzer 2200 (GE healthcare). Experiments were performed at different temperature (34°C ...
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bioRxiv - Immunology 2019Quote: ... The brain tissues were quickly dissected and single cell suspensions were prepared and separated using a 37%/70% Percoll gradient (#GE17-0891-01, GE Healthcare). Myelin was removed from the top layer and mononuclear cells were isolated from the interface ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Genomics 2019Quote: ZHBTc4 YPet-MD TIR1-V5 mCherry-OCT4-AID cells were plated in a 96-well plate coated for 1 hour at 37°C with 1:25 diluted StemAdhere (Primorigen Biosciences #S2071-500UG) and imaged on an IN Cell Analyzer 2200 (GE Healthcare) using the TexasRed and Brightfield channels ...
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Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... After cell lysis by sonication cell debris was removed by centrifugation and the lysate was passed through a His-Trap column (GE Healthcare). Proteins were eluted with a gradient of buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell pellets were resuspended in 4 ml PBS and subject to density gradient centrifugation with Ficoll-Paque PLUS (17144002, GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... YL½ antibody (IgG, expressed from hybridoma cells and purified by ion exchange chromatography) was coupled to CNBr-activated sepharose beads (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Background binding to reference flow cells was subtracted and antibody binding levels were calculated using Biacore T200 evaluation software (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... connected to a ÄKTA Pure apparatus equipped with a UV 280 nm flow cell (all from GE Healthcare, Chicago, IL, USA). EV fractions were collected based on UV absorbance ...
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bioRxiv - Microbiology 2020Quote: His-tagged SARS VHH-72 or MERS VHH-55 was immobilized to a single flow cell of an NTA sensorchip at a level of ∼400 response units (RUs) per cycle using a Biacore X100 (GE Healthcare). The chip was doubly regenerated using 0.35 M EDTA and 0.1 M NaOH followed by 0.5 mM NiCl2 ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-human Fc monoclonal antibody R10Z8E9 was immobilised onto active and reference flow cells of a Series S sensor CM5 chip using an amine capture kit (both from GE Healthcare). Approximately 1000 response units of Pf34 bait protein was captured onto the active flow cell ...