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Citations for GE Life Sciences :
5351 - 5400 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: dHepaRG or PHH cells seeded into a 24-well plate were transfected with 25 nM or 10 nM of siRNAusing Dharmafect#1 (GE HealthCare) or Lipofectamine RNAiMax (Life Technologies) ...
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bioRxiv - Cancer Biology 2020Quote: ... were diluted with PBS and layered with 35 mL of diluted cell suspension over 15 mL of Ficoll-Paque (GE healthcare) in 50 mL conical tube ...
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bioRxiv - Cell Biology 2021Quote: ... were collected by crushing of bones (tibias, femurs, hips, humeri and spine) and subjecting the cells to density gradient centrifugation (Ficoll-Paque Plus, GE Healthcare) at 400g for 25 minutes with brakes turned off ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T (ATCC CRL-3216) cell lines used in this study were cultured at 37°C in 5% CO2 in high-glucose DMEM (GE Healthcare) supplemented with 10% FBS (Atlanta Biologicals) ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were incubated with HRP-coupled secondary antibodies (santa cruz, cell signaling) for 1 h at RT and chemiluminescence detected with ECL Prime (GE Healthcare) at the LAS-1000 (Fujifilm) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Biophysics 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin and gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Physiology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from age- and sex-matched mice by centrifugation over Ficoll-Paque Plus (GE Healthcare). Splenocytes were obtained by crushing the tissue through a 70μm nylon filter (Falcon) ...
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bioRxiv - Bioengineering 2022Quote: Human adult peripheral blood mononuclear cells (PBMCs) were purified by density centrifugation using Ficoll-Paque (density = 1.077 g/mL; 600g, 30 minutes; GE Healthcare; USA). Informed written consent was given by donors to give approval for the use of waste material for research purposes ...
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Microbiology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were separated from blood samples by density gradient centrifugation using Percoll (GE Healthcare, Chicago, IL, USA). Whole blood was lysed with ACK lysing buffer to separate white blood cells ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from the blood by lymphocyte isolation (Ficoll-Paque PREMIUM; GE Healthcare, Piscataway, NJ, USA) and plated in duplicate wells ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants of cell lysate were incubated with either pre-equilibrated GFP nanobody-conjugated NHS-Activated Sepharose 4 Fast Flow Agarose (GE Healthcare) or Pierce Protein A Agarose (Invitrogen/Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... As mentioned above GFP positive HIV infected cells were enumerated by fluorescence microscopy using the high content CYTELL imaging platform (GE Healthcare). An average of 25,000 cells were analysed per condition by acquiring multiple fields of view ...
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bioRxiv - Microbiology 2020Quote: 80 μg of primary epithelial cell lysates or 1 μg purified receptor candidates separated in SDS-PAGE gels were Western-blotted onto nitrocellulose (Amersham, GE Healthcare) with Schaeffer-Nielsen buffer (48mM Tris ...
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bioRxiv - Molecular Biology 2019Quote: ... (amino acids 2-241 fused to 6 x his) were expressed in BL21 cells and purified on glutathione-linked sepharose beads (GE healthcare) or Ni-NTA-Agarose (GE healthcare ...
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bioRxiv - Microbiology 2021Quote: Purified His-tagged Pentamer was immobilized to a single flow cell of a NiNTA sensor in a Biacore X100 (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Immunology 2020Quote: ... blood samples were collected during hospitalization and follow-up visits and separated into plasma and peripheral blood mononuclear cells (PBMCs) by Ficoll-Hypaque gradient (GE Healthcare) centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... human adult peripheral blood was harvested from healthy donors after informed consent and immune cell isolation using Ficoll-Paque gradient (GE Healthcare). Buffy coat was harvested for PBMCs while granulocytes were isolated from the gradient pellet ...
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bioRxiv - Bioengineering 2020Quote: ... were isolated from Trima Accel leukoreduction system (LRS, Memorial Blood Center) chambers using ammonium chloride-based red blood cell lysis followed by Ficoll-Paque gradient (GE Healthcare). PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... mononuclear cells were isolated by density gradient centrifugation of PBS-diluted buffy coat/blood (1:2) over Ficoll-Paque Plus (GE Healthcare). The PBMC layer was carefully removed and washed 3 times with PBS.
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bioRxiv - Immunology 2022Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was expressed using Hi5 cells and purified by Ni-NTA resin followed by gel filtration chromatography (GE Healthcare) in HBS buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... and cells were resuspended in PBS-EDTA (room temperature) and layered onto Ficoll Paque (1.077 g/mL; GE Healthcare Europe GmbH). After centrifugation (800 x g ...
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bioRxiv - Microbiology 2019Quote: ... The recombinant protein in the soluble cell fraction was purified by immobilized metal affinity chromatography (IMAC) using a HiTrap Chelating HP 1-ml column (GE Healthcare) with an ÄKTA purifier FPLC system (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... G2 and G1 phases based on the signal intensity of anti-CENPF antibody staining with software (IN Cell Investigator, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Cell lysate was centrifuged at 35,000 g for 60 minutes to remove insoluble cell debris and the soluble lysate was loaded onto a 5 mL His Trap HP Ni column (GE Healthcare) at a rate of 1.5 mL/minute ...
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bioRxiv - Immunology 2019Quote: ... the digested tissues were pressed through a 40 µm nylon strainer (Falcon) and immune cells were purified via 40/70% Percoll (GE Healthcare) gradient and centrifugation.
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bioRxiv - Immunology 2020Quote: ... Purification of His-HMGB1 from 293-freestyle cell lysate was carried out using Ni SepharoseTM 6 Fast Flow gel (GE Healthcare). After purification ...
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bioRxiv - Pathology 2020Quote: ... the vector was purified from the cell lysate using a iodixanol density gradient followed by anion exchange chromatography (HiTrap Q-FF column, GE Healthcare). The scAAV9-CB-SMN vector was finally resuspended and concentrated in DPBS on a centrifugal filter unit (Amicon® Ultra-15 ...
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bioRxiv - Cell Biology 2019Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density gradient centrifugation (GE Healthcare Biosciences, 30 min, 4°C, 2,100 r.p.m.). PBMCs were extensively washed in cold phosphate buffered saline (PBS ...
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bioRxiv - Synthetic Biology 2019Quote: ... cells were lysed by three passes through an emulsiflex C5 (Avestin, Mannheim, Germany) and cleared cell lysate was loaded onto a HisTrap FF column (GE Healthcare). After washing with 10 column volumes of buffer A (30 mM Tris-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... Whole blood was diluted with room temperature RPMI medium in a 1:2 ratio to facilitate cell separation for other analyses using the SepMate PBMC isolation tubes (STEMCELL) containing 16 mL Ficoll (GE Healthcare). Diluted whole blood was centrifuged at 1200 g for 20 minutes at 20 °C ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Ficoll-Paque™ Plus (GE Healthcare Europe, Brøndby, Denmark). They were either examined directly ex vivo or cryopreserved in 10% DMSO and 90% FCS at -150°C for later in vitro analysis.
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bioRxiv - Cell Biology 2019Quote: ... as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare) using an amine coupling kit (GE healthcare). GST tagged ligand proteins were captured on the detection flow cell of the chip and free GST was captured on the reference flow cell ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Physiology 2021Quote: ... The hemolysate was then centrifuged at 4°C (12000 g, 20 min) to remove cell debris and then passed (‘stripped’) on a PD-10 desalting column (GE Healthcare) equilibrated with 10 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... TolA was loaded into the cell and synthetic TolB peptide (residues 22-33; 2 mM) loaded into the syringe of an iTC200 microcalorimeter (Microcal/GE Healthcare). Titration consisted of 20 injections (1 x 0.4 μl ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris was pelleted at 15000 × g for 10 min and 10 μg of the cleared cell lysates were resolved on a 10% SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane (GE life Sciences). Membranes were blocked in blocking buffer (5% non-fat milk ...
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bioRxiv - Microbiology 2020Quote: ... Lysed cells were ultracentrifuged and the cleared supernatant loaded onto a Ni Sepharose 6 Fast Flow His Trap column (GE Healthcare) equilibrated with Buffer A ...
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bioRxiv - Microbiology 2021Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood within 24 hours of sample collection using Ficoll-Paque (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by density gradient centrifugation on Ficoll-Paque™ PLUS (1.077 g/L; GE healthcare). Then ...
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bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...
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bioRxiv - Microbiology 2019Quote: ... lysed with 100 μg lysozyme and sonication and the filtered cell lysate loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Cell debris was pelleted by centrifugation and the soluble fraction was loaded onto a HisTrap-HP Ni-Sepharose column (GE Healthcare). Bound protein was eluted with lysis buffer supplemented with 300 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... human adult peripheral blood was harvested from healthy donors after informed consent and immune cell isolated using Ficoll-Paque gradient (GE Healthcare). Buffy coat was harvested for PBMCs while granulocytes were isolated from the gradient pellet ...
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bioRxiv - Cell Biology 2019Quote: Human his-tagged TRMT6/61A dimer was purified from Escherichia coli BL21 cells using 1 mL HisTrap HP affinity chromatography columns (GE Healthcare). Eluted fractions containing the recombinant proteins were pooled and incubated overnight with PreScission protease at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... The cells were lysed by sonication and the lysate was separated on a Ni sepharose 6 Fast Flow resin (GE Healthcare) equilibrated with 30 mM Tris-HCl pH 8 ...
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bioRxiv - Genomics 2021Quote: ... followed by IgG-affinity chromatography and finally anion-exchange using a previously described procedure (Moreno-Morcillo et al., 2014) with the following modifications: for cells lysis and equilibration of the heparin column (GE Healthcare), we used instead a buffer containing 250 mM Tris–HCl pH 8 ...