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Citations for GE Life Sciences :
5151 - 5200 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were purified using density gradient centrifugation on Percoll (GE Healthcare, Little Chalfont, UK), washed three times with phosphate-buffered saline (PBS) ...
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bioRxiv - Cell Biology 2021Quote: ... 2005) https://www.youtube.com/watch?v=ZrZVbFg9NE8).> Cells were imaged using either a DeltaVision system (Applied Precision, Issaquah, WA) or an Axio Observer (Carl Zeiss) ...
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bioRxiv - Microbiology 2020Quote: ... the cell supernatant was collected and loaded to a column packed with GammaBind Plus Sepharose beads (GE Healthcare). The column was washed with PBS ...
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bioRxiv - Cancer Biology 2021Quote: Primary BCP-ALL and normal bone marrow mononuclear cells were purified using Ficoll (#17144002, GE Healthcare, Pittsburgh, PA). Human specimen collection protocols were reviewed and approved by Children’s Hospital Los Angeles Institution Review Board (IRB ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMC) were isolated by Ficoll-Paque density gradient centrifugation (GE Healthcare Europe, Velizy, France). PBMC were immediately processed for flow cytometry analysis or frozen in fetal bovine serum/7.5% DMSO (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) and plasma were obtained by standard density gradient centrifugation over Ficoll (GE Healthcare). PBMC were frozen in 10% DMSO/FBS and stored in liquid nitrogen until analysis ...
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bioRxiv - Immunology 2020Quote: ... peripheral blood mononuclear cells (PBMCs) were separated from buffy coats by density gradient centrifugation (Ficoll Paque, GE Healthcare). NK cells were then isolated from PBMCs by negative selection according to manufacturer’s instructions (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were subjected to a density gradient centrifugation using a 37% Percoll solution (GE Healthcare Life Sciences). Erythrocytes were lysed with NH4Cl.
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired every 10 min for 12 h using an IN Cell Analyzer 2000 microscope (GE Healthcare; Nikon 20x/0.45 NA Plan Fluor objective ...
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bioRxiv - Immunology 2022Quote: ... Austria) and peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation (Ficoll-Paque, GE Healthcare, ThermoFisher). The PBMCs were washed and cryopreserved in freezing medium (8% DMSO in FBS ...
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bioRxiv - Immunology 2022Quote: ... LP immune cells were enriched at the interface of a 40%/80% Percoll gradient (GE Healthcare Life Sciences), washed and stained for flow cytometry with the same antibodies used in whole blood ...
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bioRxiv - Microbiology 2024Quote: ... and the cells were used for RNA extraction using the Illustra RNAspin Mini RNA isolation kit (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell-free supernatant was applied to the column at 2 mL/min using an AKTA Pure (GE Healthcare), washed ...
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bioRxiv - Immunology 2024Quote: Peripheral blood mononuclear cells (PBMCs) from COVID-19 patients were isolated from the blood using Ficoll (GE Healthcare) density gradient media and transferred into 96-well plates at a concentration of 2.5 × 106 viable cells per ml in 200µl (0.5 × 106 cells per well ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20x objective (GE Healthcare). Images were batch processed through an imaging processing software ...
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bioRxiv - Neuroscience 2023Quote: ... The collected cell suspension was mixed with isotonic Percoll (GE Healthcare, 90% Percoll 10% 10x PBS, pH 7.2) at a final concentration of 22.5% and centrifuged at 800 g for 20 min without break at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20X objective (GE Healthcare). Neuronal mitoSOX signal was detected based on the overlap of mitoSOX with TUBB3 ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from the buffy coats with Ficoll-Paque (GE Healthcare, Pittsburgh, PA). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were mixed 1:1 with phosphate-buffered saline (PBS) and layered on Ficoll–Paque (GE Healthcare; 1714402). Cells were spun at 800 g for 30 min ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMCs) from COVID-19 patients were isolated from the blood using Ficoll (GE Healthcare) density gradient media and transferred into 96-well plates at a concentration of 2.5 × 106 viable cells per ml in 200µl (0.5 × 106 cells per well ...
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bioRxiv - Cancer Biology 2022Quote: ... Immune cells were isolated using a Ficoll gradient as described by the manufacturer (GE Healthcare, GE17-0891-01). The resulting immune cells were resuspended in 1x PBS prior to being plated in a 96-well plate for flow staining.
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells were isolated from buffy coats by density centrifugation using Ficoll-Paque Plus (GE Healthcare). CD14+ monocytes were subsequently isolated using magnetic-activated cell sorting (Miltenyi Biotech ...
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bioRxiv - Cancer Biology 2022Quote: ... Ptch;p53 primary cell-lines (#9410) were transfected with 40 nM ON-TARGETplus mouse Yap1 siRNA (GE Healthcare Dharmacon ...
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bioRxiv - Cell Biology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated from the blood samples using Ficoll-Paque (GE Healthcare, Cat# 17144003) and gradient centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Antibody purification from cell supernatants was done in two steps using the ÄKTA Avant purification system (GE Healthcare). The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe ...
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bioRxiv - Immunology 2023Quote: ... Hepatic parenchymal cells were removed by density gradient centrifugation at 700 × g in 33.75% Percoll PLUS (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Cell Biology 2023Quote: ... cells were imaged using a 60x oil immersion objective lens on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... High-throughput imaging was carried out using the In Cell Analyzer 2000 with a 20X objective (GE Healthcare). Neuronal γH2AX signal was detected based on the overlap of γH2AX with TUBB3 ...
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bioRxiv - Molecular Biology 2023Quote: ... washed three times with PBS and then imaged in PBS with the IN Cell Analyzer 2200 (GE Healthcare) at 4x magnification ...
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bioRxiv - Microbiology 2024Quote: ... The infection was assessed by using an IN CELL Analyzer 6500 high-throughput automated confocal microscope (Ge Healthcare) located in BSL-3 safety laboratory ...
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bioRxiv - Cell Biology 2024Quote: Live-cell fluorescence imaging was performed using a Delta Vision Elite widefield fluorescence microscope (GE Healthcare Life Science) equipped with a 100× PlanAPO oil-immersion objective lens (Olympus ...
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bioRxiv - Cancer Biology 2024Quote: ... Mononuclear cells were extracted from bone marrow samples using Ficoll-Paque™ PLUS (GE Healthcare #17-1440-02), followed by red blood cell lysis using a red blood lysis buffer (Sigma #R7757).
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bioRxiv - Biochemistry 2024Quote: ... Clear cell lysates (clarified by centrifugation) were purified with Ni-HiTrap FF and MBPtrap HP columns (GE Healthcare). Then tag-cleaved proteins was purified with ion exchange chromatography (HiTrap SP FF column (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were imaged using a UPLSAPO 100x / 1.4NA oil objective on a DeltaVision deconvolution microscope (GE Healthcare, UK) equipped with an IX71 inverted microscope (Olympus ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence images were acquired with an API DeltaVision Live-cell Imaging System (Applied Precision Inc., GE Healthcare Co., U.S.A.) and were captured at x60 magnification ...
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bioRxiv - Cell Biology 2019Quote: ... Fluorescence images were acquired with an API DeltaVision Live-cell Imaging System (Applied Precision Inc., GE Healthcare Co., U.S.A.) and were captured at x60 magnification ...
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bioRxiv - Cell Biology 2020Quote: ... cells were visualized live using the GFP or mCherry filter set of the DeltaVision Elite (GE Healthcare, Pittsburg, PA).
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bioRxiv - Immunology 2021Quote: ... single cell suspension of thymi were subjected to density-gradient centrifugation with a three-layer Percoll gradient (GE Healthcare) with gravities of 1.115 ...
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bioRxiv - Cell Biology 2020Quote: ... Fluorescence microscopy of live (time-lapse movies) and fixed cells were acquired using a Deltavision microscope system with softWoRx 4.1.2 software (Applied Precision), 100× 1.4 NA UPlanSApo oil immersion objective ...
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bioRxiv - Physiology 2022Quote: ... Buffy coat preparations of peripheral blood mononuclear cells (PBMCs) were isolated by centrifugation over Ficoll-Paque Plus (GE Healthcare). Cell pellets of PBMCs were snap frozen prior to analysis for unprenylated proteins.
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bioRxiv - Molecular Biology 2020Quote: ... All GST-B3 fusion proteins were expressed in E.coli BL21(DE3) cells and were purified by gluthathione-Sepharose affinity chromatography according to the manufacturer’s recommendations (GE Healthcare). The proteins were eluted in elution buffer (20 mM reduced glutathione ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were recorded by acquiring one field of view per well in a 96-well black plate (GE healthcare). We used our previously developed semi-automated pipeline for segmentation and tracking of individual cells5.
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bioRxiv - Genomics 2019Quote: ... for 10 minutes at room temperature and subsequently washed and imaged on an IN Cell Analyzer 2200 (GE Healthcare). Images were background-subtracted using FiJi (Schindelin et al. ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Clarified cell supernatants were concentrated 10-fold using Kicklab tangential flow filtration cassettes (GE Healthcare, cut-off 10 kDa) and adjusted to pH 8.0 in 10 mM Tris before purification in an AKTA Avant system by Steptrap-HP chromatography (GE Healthcare) ...
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bioRxiv - Immunology 2021Quote: ... All primary antibody incubations were followed by incubation with appropriated secondary HRP-conjugated antibodies (GE Healthcare or Cell Signaling) in 5% milk plus 0.1% Tween20 (Sigma P2287) ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary CD8+ and CD4+ T cells were isolated from buffy coats form healthy donors by Ficoll gradient (GE Healthcare) followed by negative selection with RosetteSep Human CD8+ T Cell Enrichment Cocktail or RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (GE Healthcare, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml of streptomycin (PS ...
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bioRxiv - Neuroscience 2019Quote: ... cells were harvested and rAAVs were purified using 1 mL HiTrap heparin columns (GE Healthcare Bio-Sciences, Uppsala, SwedenSigma) and concentrated using Amicon Ultra centrifugal filter devices (Merck Millipore ...