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Citations for GE Life Sciences :
5001 - 5050 of 5577 citations for Dengue Virus Serotype 3 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The soluble cell lysates were applied on a Ni-sepharose excel (GE Healthcare, #17-3712-01), packed in a column of 4 ml bed volume with Sl1 buffer buffer (20mM Tris ...
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bioRxiv - Genomics 2023Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation over Ficoll-Paque (GE Healthcare) followed by multiple washes in PBS/EDTA (Miltenyi Biotec) ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates prepared by sonication and centrifugation were purified on a HisTrap HP column (GE Healthcare). The N-terminal polyhistidine-GST tag was cleaved by incubation with TEV protease at 4 °C overnight ...
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bioRxiv - Biophysics 2023Quote: ... clarified lysate obtained from the cells was bound to a HiTrap Chelating HP column (GE Healthcare) in low imidazole buffer (50 mM PIPES ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were grown overnight at 37°C on nitrocellulose membranes (Amersham™ Protran™, GE Healthcare) in plates containing ZYP agar medium (supplemented with 100 µg.mL-1 ampicillin and 34 µg.mL-1 chloramphenicol) ...
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bioRxiv - Immunology 2023Quote: ... Hepatocytes were separated from immune cells by a 35% and 70% bilayer Percoll gradient (GE Healthcare) and used.
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bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
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bioRxiv - Immunology 2023Quote: Peripheral blood mononuclear cells (PBMC) were isolated from whole blood by Ficoll-Hypaque centrifugation (GE Healthcare) and processed according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: HTB2-mCherry expressing cells were imaged at room temperature using a DeltaVision (pDV) microscope (Applied Precision) equipped with a CoolSNAP HQ2/HQ2-ICX285 camera using a 60× oil objective (U-Plan S-Apochromat-N ...
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bioRxiv - Biochemistry 2023Quote: ... The cell extract was applied onto a HisTrap™ FF crude column (5 ml; GE Healthcare) pre-equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation (Ficoll-Histopaque; GE Life Sciences) from blood collected in ACD tubes ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 20.000 alive/mCD45-/hCD45+/hCD19+ cells were sorted into a PBS (GE Healthcare Life Sciences) + 2% FBS (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... when cells were transfected with either 100 nmol/L siRNA targeting FADS1 (siFADS1, GE Healthcare Dharmacon), or the respective negative controls (non-targeting small interfering RNA ...
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bioRxiv - Microbiology 2023Quote: ... Cells were viewed at room temperature with a DeltaVision Core microscope system (Applied Precision/GE Healthcare). Seven planes were acquired every 200 nm and the data were deconvolved using SoftWorx software as described previously (66) ...
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bioRxiv - Systems Biology 2023Quote: ... Time-lapse microscopy images were acquired using an IN Cell Analyzer 2500HS (GE Healthcare Life Science) with a CFI S Plan Fluor ELWD dry objective lens 20× (NA ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were subsequently imaged for 8 hours on a DeltaVision Elite imaging station (GE Healthcare), equipped with a CoolSNAP HQ2 camera ...
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bioRxiv - Neuroscience 2023Quote: ... Single cell suspensions were prepared and centrifuged over a 37%/70% discontinuous Percoll gradient (GE Healthcare), mononuclear cells were isolated from the interface ...
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bioRxiv - Cancer Biology 2023Quote: The cell proliferation rate was evaluated using the Kit Biotrak ELISA System (GE Healthcare, Orsay, France) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Image acquisition was done with the InCell Analyzer 6000 Cell Imaging System (GE Healthcare Life Sciences) and micrographs were assembled using Adobe Photoshop 2022.
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bioRxiv - Molecular Biology 2023Quote: ... The viability was quantified by staining the yeast cells in 0.1% Trypan blue staining (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by density centrifugation using Ficoll-Paque Plus (GE Healthcare). Plasmacytoid dendritic cells and monocytes were isolated using anti-BDCA4 or anti-CD14 magnetic microbeads (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... Dynein complexes were captured from Sf9 cell lysates using IgG Sepharose 6 FastFlow beads (GE Healthcare), labeled with SNAP-Cell-TMR-Star ...
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bioRxiv - Microbiology 2023Quote: ... Cells were viewed at room temperature with a DeltaVision Core microscope system (Applied Precision/GE Healthcare). Seven planes were acquired every 200 nm and the data were deconvolved using SoftWorx software as described previously (66) ...
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bioRxiv - Cancer Biology 2024Quote: Time-lapse microscopy images were acquired using an IN Cell Analyzer 2500HS (GE Healthcare Life Science) equipped with a CFI S Plan Fluor ELWD dry objective lens 20× (NA ...
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bioRxiv - Neuroscience 2024Quote: ... Supernatant was removed and cell pellets were re-suspended in 30% isotonic Percoll (GE Healthcare, #17089102). Cell suspensions were centrifuged for 20 min at 2000g ...
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bioRxiv - Immunology 2024Quote: ... human peripheral blood mononuclear cells were recovered by gradient centrifugation on Ficoll-Paque Plus (GE Healthcare) and monocytes were further purified using CD14 microbead positive selection and MACS separation columns (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2020Quote: ... Dead cells and debris were then removed by centrifugation in 37.5% Percoll (GE Healthcare, 17-0891-02) at 2000g for 20 minutes ...
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bioRxiv - Molecular Biology 2020Quote: All live cell imaging was carried out on a DeltaVision OMX™ V4 imaging system (GE Healthcare), equipped with a 60x (NA 1.42 ...
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bioRxiv - Microbiology 2020Quote: ... 0.2 μm optical slices encompassing the entire cell were captured and deconvolved with softWoRx 5.0 (Applied Precision). From the deconvolved image stack ...
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bioRxiv - Microbiology 2019Quote: ... Cells were imaged 24 hours post-transfection on the DeltaVision LIVE high resolution deconvolution microscope (GE Healthcare) using the 60X/1.4 Plan-Apo NA oil objective (Olympus) ...
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bioRxiv - Immunology 2020Quote: Brain tissue from the animals was collected and NK cells were isolated using a percoll (GE Healthcare) gradient fallowing protocol described by Pino et al 57 ...
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bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
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bioRxiv - Developmental Biology 2019Quote: ... Whole heart acquisitions were obtained using the high-content imaging system (IN Cell Analyzer 2000, GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... moDC were gently washed twice with T cell expansion medium (RPMI supplemented with HyClone FBS [GE Healthcare, Chicago ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were supplemented with 10 mM imidazole and incubated with Ni-Sepharose excel resin (GE Healthcare) for 2 that 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was centrifuged and the supernatant was incubated with Ni-NTA affinity resin (GE Healthcare). The target protein was eluted with lysis buffer supplemented with 300 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... The cell-free lysate was applied into a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated in start buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Two days post-transduction cells were imaged using the OMX (Applied Precision, Issaquah, WA 98027, V3 (3.20.3537.0), vintage 2010 ...
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bioRxiv - Cancer Biology 2020Quote: ... Mononuclear cells (MNC) were extracted from fresh BM using Ficoll-Paque Plus (GE Healthcare, #17-1440-02). Bone marrow MNCs were also extracted from two patients (ALL10 and ALL12 ...
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bioRxiv - Cancer Biology 2020Quote: ... All breast cancer cell lines were maintained in Dulbecco’s Modification of Eagle’s Medium (DMEM, GE Life Sciences) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Biochemistry 2020Quote: ... After cell disruption the cleared lysate was loaded on a 5 ml HisTrap FF column (GE Healthcare) equilibrated in lysis buffer A and the protein was eluted with a gradient of 25 – 250 mM imidazole ...
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bioRxiv - Immunology 2020Quote: ... Fresh peripheral blood mononuclear cells (PBMCs) were obtained from buffy coats by Ficoll Paque Plus (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells were obtained by Ficoll gradient separation on Ficoll-Paque (GE Healthcare, Amersham, UK). Then ...
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bioRxiv - Microbiology 2020Quote: ... Following the isolation of peripheral mononuclear cells (PBMC) by overlaying blood on Ficoll-paque plus (GE Healthcare), the serially diluted GA-inactivated SARS-CoV-2 samples were incubated with the PBMCs for 48hr in T cell medium (6% Human AB Serum and 1% Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... images were acquired on a IN Cell 2200 automated high-throughput fluorescence microscope (GE Healthcare, Chicago, USA) using IN Cell Analyzer (version 6.2 ...
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bioRxiv - Cancer Biology 2020Quote: ... while HCT-15 and HL-60 cells were grown in RPMI medium (Hyclone; GE Healthcare Life Sciences) containing 10% fetal bovine serum (TianJin Haoyang Biological Products Technology Co. ...
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bioRxiv - Genomics 2021Quote: ... Btbd7- and Zcchc8-knockout SIMS cells were maintained in phenol red-free DMEM (GE Healthcare/Cytiva, SH30284.01) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2022Quote: Cell extract samples were separated on 12% SDS-PAGE gels before transfer to nitrocellulose membranes (GE Healthcare). Membranes were blocked in PBS containing 5% milk before probing with primary antibodies at concentrations listed in S2 Table ...
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bioRxiv - Cancer Biology 2022Quote: 293T cells were cultured in normal DMEM media supplemented with 10% bovine growth serum (GE Healthcare, SH30541.03) and 1% penicillin-streptomycin (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were further purified by centrifugation over a Percoll gradient (GE Healthcare, 17-0891-01, Sigma Aldrich).