Labshake search
Citations for Quantifoil Micro Tools :
651 - 700 of 784 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: The final high resolution data set was collected across 2 grids (Quantifoil 1.2/1.3 200 mesh) prepared with 1.8 mg mL-1 trimeric complex and 0.5 and 0.1 X CMC CHAPS ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh copper grids with a 2 nm continuous carbon layer (Quantifoil) were plasma-treated in a Pelco easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Biophysics 2024Quote: ... Vero cells were seeded either on gold finder grids (200 mesh, R2/2, Quantifoil, Jena, Germany) or gold grids coated with lacey carbon film (XXBR Technology ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Three-hundred mesh R1.2/1.3 UltrAuFoil grids with a layer of 2 nm amorphous carbon (Quantifoil) were glow discharged in a Pelco easiGlow for 12 seconds under a 0.37 mBar vacuum with 25 mA current ...
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bioRxiv - Biophysics 2020Quote: Three microliters of the apoferritin samples with 1.5 mg/ml or 0.2 mg/ml were applied onto glow-discharged 200-mesh R2/1 Quantifoil grids (used for the K3 dataset) or 200-mesh R2/1 Quantifoil grids coated with continuous carbon film (Quantifoil, used for the Falcon4 dataset), respectively ...
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bioRxiv - Biophysics 2024Quote: ... to produce a fiducial marker along with 0.01% NP-40 and 10 μM tubulin solution with BRB80 buffer and 1 mM GTP before the solution was dropped onto the grids (QUANTIFOIL R 1.2/1.3 Cu 200). The grids were blotted with vitrobot (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Aliquots (4 μL) of the protein complex were placed on glow-discharged holey carbon grids (Quantifoil Au R1.2/1.3, 300 mesh). The grids were blotted and flash-frozen in liquid ethane cooled by liquid nitrogen with a Vitrobot apparatus (Mark IV ...
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bioRxiv - Microbiology 2020Quote: Three μl of purified HKU1 HE (4 mg/ml) was dispensed on Quantifoil R1.2/1.3 200-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 20 mA using GloQube Glow Discharge system (Quorum Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 μL of protein samples were immediately applied to discharged 300-mesh Au R1.2/1.3 grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4 s and plunged into liquid ethane using an FEI Mark IV Vitrobot operated at 8°C and 100% humidity ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μl of the sample was applied to glow discharged holey carbon-coated 200 mesh copper EM grids (Quantifoil Micro Tools), vitrified as above at 4°C and 100 % humidity.
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bioRxiv - Immunology 2024Quote: Neutrophils were stimulated with 100 nM LTB4 in mHBSS for 15 mins at 37 °C in 35 mm glass bottom dishes containing glow-discharged Au SiO2 R1/4 200 mesh grids (Quantifoil GmbH) precoated with 25 μg/ml fibrinogen in DPBS ...
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bioRxiv - Immunology 2024Quote: ... a 4 μL aliquot of protein complex was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300-mesh) or graphene oxide (GO)-coated holey-carbon gold grids (EMR ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of the h20S-PA26E102A-Opt5 solution were applied to R2/2 400-mesh grids (Quantifoil) that had been plasma treated for 25 s using a glow discharger (Electron Microscopy Sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 3.5 μL was applied to freshly glow-discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biophysics 2020Quote: ... 2 μl apoferritin was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil). Grids were blotted for 4 seconds at -2 force and vitrified in liquid ethane using a MarkIV Vitrobot (ThermoFisher) ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL was applied to freshly glow discharged Quantifoil R2/2 300-square-mesh gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Microbiology 2023Quote: ... The samples were applied to a Quantifoil R2/2 Cu 300 mesh grid (Quantifoil Micro Tools GmbH), which had been freshly glow-discharged for 60 s at 10 mA using PIB-10 (Vacuum Device) ...
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bioRxiv - Microbiology 2024Quote: A purified filament fraction (2 µL) was applied to a copper grid (R1.2/1.3 300 mesh; Quantifoil) and glow-discharged for 10 s using a JEC-3000FC Auto Fine Coater (JEOL) ...
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bioRxiv - Biochemistry 2024Quote: Samples were concentrated to 0.2 mg/ml or 1 mg/ml and prepared either on copper grids with holey carbon support and an additional layer of 2 nm continuous carbon (R1.2,1.3, Quantifoil) or on UltAufoil grids (R0.6/1.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... bremelanotide– or THIQ–bound MC4R–Gs complexes at ∼ 4 mg/mL were applied onto glow-discharged holey carbon grids (Quantifoil R1.2/1.3). Excess samples were blotted away and the grids were vitrified by plunging into liquid ethane using a Vitrobot Mark IV (ThermoFischer Scientific).
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bioRxiv - Genomics 2020Quote: ... Vitrified specimens were prepared by adding 4 μl disome samples at the concentration of ~ 150 nM to a glow-discharged holey carbon grid (Quantifoil R1.2/1.3), which was covered with a freshly made thin carbon film ...
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bioRxiv - Cell Biology 2020Quote: ... The MEFs were applied onto glow-discharged EM grids with a silicon-oxide support film (R1/4, Au mesh; Quantifoil, Jena, Germany). After incubation ...
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bioRxiv - Cell Biology 2022Quote: The cryo-EM grid was prepared by applying 3 μl of the protein samples (4 – 6 mg/ml) to glow-discharged Quantifoil R1.2/1.3 300-mesh gold holey carbon grids (Quantifoil, Micro Tools GmbH, Germany). Grids were blotted for 4.0 seconds under 100% humidity at 4°C before being plunged into the liquid ethane using a Mark IV Vitrobot (FEI) ...
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bioRxiv - Cell Biology 2023Quote: ... Four µL of the cells were subsequently deposited onto Quantifoil R2/4 200 mesh copper grids (Quantifoil Micro Tools GmbH, Jena, Germany), which were then manually blotted from the back with Whatman® Grade 1 filter paper for approximately 3 – 5 seconds ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl suspended cells were loaded onto the front side of glow-discharged holy-carbon copper grids (Quantifoil R1.2/1.3, 200 mesh), and 2 μl medium was subsequently added to the back side of the grids ...
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bioRxiv - Biochemistry 2020Quote: ... and 2 µL sample was applied to glow-discharged gold holey carbon 1.2/1.3 300-mesh grids (Quantifoil) coated in-house with graphene oxide55-57 ...
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bioRxiv - Biochemistry 2021Quote: ... A volume of 2.5 μL of each sample was applied on glow-discharged UltrAuFoil Au300 Rl.2/1.3 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...
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bioRxiv - Biochemistry 2022Quote: ... 2 μl retromer at a concentration between 80-100 μg/ml was applied to freshly glow discharged CF-2/2-2C C-Flat grids (Protochips, Morrisville, NC) or Quantifoil R 1.2/1.3 300 mesh copper grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2022Quote: ... 3.5 μL of cell suspension was applied on glow-discharged copper or gold EM grids (R2/2, Quantifoil) and automatically back-blotted (Weiss et al 2017 ...
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bioRxiv - Evolutionary Biology 2021Quote: Molybdenum R1.2/1.3 and R2/2 200-mesh grids with a holey carbon support film (Quantifoil, Jena, DE) were glow discharged for 40 s under high vacuum shortly before sample application ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 2/1 200 mesh Cu ...
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bioRxiv - Biochemistry 2021Quote: ... 2 uL of crystal slurry was applied to each side of a glow-discharged holey carbon grid (Quantifoil, R 1/4 300 mesh Cu ...
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bioRxiv - Cell Biology 2020Quote: ... K735 apo and K772-ANP datasets were collected on untreated carbon/copper grids (Quantifoil R2/2 300 mesh). The grids used for the other 15 datasets were gold grids (UltrAuFoil R2/2 200 mesh) ...
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bioRxiv - Microbiology 2022Quote: Phage particles were negatively stained for 20 s with 2 % uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2023Quote: ... Phage particles were negatively stained for 20 seconds with 2% uranyl acetate on carbon-coated copper grids (Quantifoil) and observed at 100 kV on a Hitachi HT 7700 equipped with an AMT XR81B Peltier cooled CCD camera (8M pixel ...
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bioRxiv - Microbiology 2022Quote: ... the amoebae (5×105 per well) were seeded onto EM gold finder grids (Au NH2 R2/2, Quantifoil) and incubated for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then seeded onto gold grids (either ‘UltrAuFoil’ 200 Au mesh, R2/2 gold film, or Quantifoil 300 Au mesh ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 μl of KMN complex at 0.3 mg/ml supplemented with 0.003% Triton were applied to glow-discharged UltrAuFoil R2/2 grids (Quantifoil). Excess liquid was removed by blotting (3.5 s at blot force −3) ...
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bioRxiv - Biochemistry 2023Quote: ... Differentiated U937 cells became adhesive and were seeded onto gold London Finder grids (Au H2 R2/2, Quantifoil) coated with vitronectin (25 µg/mL in 1xPBS ...
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bioRxiv - Biophysics 2024Quote: ... Regions of the LCP drops were scooped and gently smeared across pre-clipped Cu200 R2:2 grids (Quantifoil) under a humidifier ...
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bioRxiv - Biochemistry 2024Quote: About 3 µl of the purified complex sample was applied to a freshly-glow discharged Au 300 mesh R1.2/1.3 grids coated with 2 nm carbon film (Quantifoil). The grids were blotted with force level of 1 and blot time of 4s ...
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bioRxiv - Microbiology 2024Quote: Cryo-ET samples were prepared using copper grids with holey carbon support film (300 mesh, R2/2, Quantifoil) as previously described116 ...
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bioRxiv - Microbiology 2024Quote: ... 3.5 μL of protein sample at 0.15 mg/mL for the Gal/GalNAc lectin and at 0.2 mg/mL for lectin:scFv complexes were applied onto glow-discharged grids (Quantifoil R2/1 carbon film on a 300 gold mesh). After incubation for 10 s ...
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bioRxiv - Biochemistry 2021Quote: 3.5 µl protein solution containing 0.05 µM CUL4-NEDD8/ROC1/DDB1/DCAF1-CtD/Vprmus/SAMHD1 complex and 0.25 µM UBCH5C-ubiquitin conjugate (S4 A, B Fig) were applied to a 300 mesh Quantifoil R2/4 Cu/Rh holey carbon grid (Quantifoil Micro Tools GmbH) coated with an additional thin carbon film as sample support and stained with 2% uranyl acetate for initial characterisation ...
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bioRxiv - Biochemistry 2022Quote: ... Spike trimer - Fab complexes (4 μl) were applied to the pre-glow-discharged holey carbon grids (Quantifoil grid, Cu 300 mesh, R1.2/1.3). The grids were then blotted for 2 seconds with filter paper in 100% relative humidity and 8 °C and plunged into the liquid ethane to freeze samples using FEI Vitrobot system (FEI).
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of 4 μL protein sample of Spike protein was applied onto a glow-discharged 300 mesh grid (Quantifoil Au R1.2/1.3) and GraFuture™-RGO grid which was supported with a thin layer of RGO (reduced graphene oxide) ...
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bioRxiv - Biochemistry 2023Quote: 4 μL of Fcα–J–FcRL4 (0.3 mg/mL) were applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh) or Ni-Ti grids (CryoMatrix ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were grown for 24 h on holey carbon-covered gold EM grids (200 mesh, R2/2, Quantifoil), fed with oleic acid and induced with doxycycline for Fsp27-EGFP expression for an unrelated project ...
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bioRxiv - Biophysics 2020Quote: Cryo-EM grids were prepared by applying freshly purified channel protein (~3.5 μl) to glow-discharged copper Quantifoil R2/2 holey carbon grids (Quantifoil), which were then blotted for 2 s with ~100% humidity and flash frozen in liquid ethane using FEI Vitrobot Mark IV (FEI) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were grown on 200 mesh gold R2/2 London Finder Quantifoil grids (Quantifoil Micro Tools GmbH, Jena, Germany). Prior to seeding cells ...