Labshake search
Citations for Quantifoil Micro Tools :
601 - 650 of 784 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Protein-DNA complexes were applied to ultra-gold foil grids with holey gold support and 300 mesh size (R 0.6/1,Quantifoil Micro Tools GmbH); grids were flash-frozen as described [21] and were stored in liquid nitrogen until use.
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bioRxiv - Biochemistry 2024Quote: Samples were prepared by applying the polymerized R-β-Actin actin to freshly glow-discharged Quantifoil 3.5/1 on 200 mesh copper grids (Quantifoil GmbH, Germany), using a Leica GP2 (Leica Microsystems GmbH ...
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bioRxiv - Biochemistry 2020Quote: Quantifoil R2/2 300 mesh holey carbon copper grids (Quantifoil Micro Tools, Großlöbichau, Germany) were glow-discharged (ELMO Glow Discharge System ...
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bioRxiv - Biochemistry 2024Quote: ... Three microliters of sample were applied to glow-discharged R2/2 carbon grids (Quantifoil), which were blored for 5 s and at a blot force of 25 and plunge-frozen in liquid ethane with a Vitrobot Mark IV (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantifoil R1.2/1.3 400-square mesh Cu grids with 2 nm continuous carbon (Quantifoil) were glow-discharged for 8 s at 10 mA using a PELCO easiGlow glow discharging system (Ted Pella ...
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bioRxiv - Cell Biology 2022Quote: MEF were seeded on R2/2 holey carbon on gold grids (Quantifoil or Protochips) coated with fibronectin in a glass bottom dish (Mattek or Ibidi ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were seeded on EM grids (Quantifoil SiO2 R2/2, 200 mesh, gold) in a 3D printed specimen holder [43] and grown overnight.
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bioRxiv - Biochemistry 2023Quote: ... Quantifoil R2/2 Cu 400 mesh holey carbon grids (Quantifoil Micro Tools, Jena, Germany) coated with a 0.4 mg/ml graphene oxide suspension (Sigma-Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... gold grids coated with holey carbon film (300 mesh, R2/2, Quantifoil, Jena, Germany) were glow-discharged and UV-treated for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded onto holey carbon gold EM grids (200 mesh, R2/2, Quantifoil) coated with 10 mg/ml fibronectin ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of aldolase sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 6 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 4 µL of apoferritin sample were applied on UltrAuFoil R1.2/1.3 300-mesh grids (Quantifoil Micro Tools GmbH) that had been freshly plasma cleaned for 7 seconds at 15 Watts (75% nitrogen/25% oxygen atmosphere ...
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bioRxiv - Biophysics 2021Quote: The LlCsm RNP and RNP-target RNA samples at approximately 0.5 mg/ml were separately applied in 4 μL volume onto glow-discharged UltrAuFoil 300 mesh R1.2/1.3 grids (Quantifoil), blotted for 3 s at 88% humidity and flash-frozen in liquid ethane using FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2022Quote: ... for 15 min before 4 μL of sample was applied to a graphene oxide coated 1.2/1.3 UltrAuFoil grids (Quantifoil) at 4°C under 100% humidity86 ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of 4 μL samples were applied to glow-discharged holey carbon girds (Cu, R1.2/1.3, 300 mesh, Quantifoil). The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... 3.5 µl cell suspension applied on glow-discharged (Pelco easiGlow) R1/4 SiO2 gold 200 mesh grids (Quantifoil), blotted for 2 sec from the backside and was plunge-frozen on a Leica EM GP2 plunger at 20 °C ...
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bioRxiv - Biochemistry 2022Quote: ... An aliquot of 3.5 μl of sample was placed on the glow-discharged cryo-EM grid (Quantifoil R1.2/1.3 or Quantifoil R2/1), blotted and plunge-frozen in liquid ethane using a Mark VI Vitrobot instrument maintained at 100% humidity with blot force 20 and blot time of 3 seconds ...
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bioRxiv - Molecular Biology 2021Quote: ... DRD1-SKF81297-LY3154207-miniGs-Nb35 complex at the concentration of 13 mg mL−1 were applied individually onto glow-discharged holey carbon grids (Quantifoil, Au200 R1.2/1.3) in a Vitrobot chamber (FEI Vitrobot Mark IV) ...
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bioRxiv - Biochemistry 2022Quote: Three microliters of Ct80S-RAC pull-out sample at 200 nM concentration was applied on holey carbon grids (Quantifoil R2/1 grid, Quantifoil Micro Tools, GmbH) and plunged-frozen into liquid ethane using a Vitrobot (FEI) ...
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bioRxiv - Microbiology 2020Quote: Usually 5 μL of liquid culture (OD680 ~ 0.3) was pipetted onto holey carbon-coated copper grids (R2/1, 200 mesh, Quantifoil Micro Tools, Jena, Germany). The grids were blotted from the reverse side and immediately plunged into the liquid ethane at liquid nitrogen temperature atmosphere using a Vitrobot ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 microliters of the purified virus sample was pipetted onto a glow-discharged R2/1 Quantifoil grid (Quantifoil Micro Tools GmbH, Jena, Germany), blotted for 2.5 seconds ...
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bioRxiv - Microbiology 2023Quote: 3 µL of the purified FL or truncated RSV NC sample were applied to a glow-discharged R2/1 300 mesh holey carbon copper grid (Quantifoil Micro Tools GmbH) and plunge-frozen in liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Biochemistry 2024Quote: ... 3.5 μL of this protein-DNA mix were applied to 300-mesh copper R2/1 Quantifoil Holey Carbon Grids (Quantifoil Micro Tools GmbH) that had been freshly glow discharged for 30 seconds at 25 mA in a GloQube Glow Discharge System (Quorum Technologies ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl of bacteria diluted to OD = 0.15 in PBS were deposited on a glow-discharged Quantifoil R2/1 holey carbon grids (Quantifoil Micro Tools GmbH, Germany) and 3 µl gold particles (10 nm BSA tracer ...
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bioRxiv - Biochemistry 2022Quote: ... three microliters of concentrated phage particles were added to a freshly glow-discharged Quantifoil R2/1 grid (Quantifoil Micro Tools GmbH, Großlöbicha, Germany) and plunge-frozen with a Vitrobot Mk IV into a 50:50 mixture of liquid ethane:propane52
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bioRxiv - Microbiology 2022Quote: Three-microliter aliquots of all AdSDV capsid populations (~1 mg/mL) were applied to glow-discharged Quantifoil holey carbon grids with a thin layer of carbon (Quantifoil Micro Tools GmbH) and vitrified using a Vitrobot Mark IV (FEI ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... were applied to glow-discharged (25 mA, 10 s) QuantiFoil Gold R 0.6/1 300-mesh holey carbon grids (QuantiFoil, Micro Tools GmbH, Germany), blotted for 4.5 s ...
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bioRxiv - Biochemistry 2020Quote: ... KBP was diluted to 0.3 mg/ml in KBP dilution buffer and 4 μl were applied to glow-discharged 1.2/1.3 AuFoil gold grids (Quantifoil®). For the third approach ...
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bioRxiv - Microbiology 2022Quote: ... A 4□μl volume of SsoCsm complex was applied to holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh) covered by a graphene oxide layer (27) ...
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bioRxiv - Biophysics 2024Quote: ... For plunge freezing the cells were pipetted (4 μl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Cell Biology 2024Quote: ... For plunge freezing the cells were pipetted (4 µl) on EM grids (gold/200mesh/R2.2 either UltrAufoil or Quantifoil) in a humidified chamber (98% RH) ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied onto freshly glow-discharged 200 square mesh Ultrafoil R2/2 gold grids (Quantifoil). The samples were plunge-frozen with a FEI Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Freshly glow-discharged finder gold quantifoil grids (200 mesh R2/2 – Quantifoil MicroTools GmbH, Germany) were sterilized in 70% EtOH and placed in a glass-bottomed MatTek dish (MatTek Corporation ...
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bioRxiv - Microbiology 2021Quote: ... were applied to freshly washed and glow-discharged R2/2 holey carbon copper grids (Quantifoil). The grids were manually back blotted for 2 to 3s with filter paper ...
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bioRxiv - Biochemistry 2021Quote: ... Cryo-EM grids were prepared using glow discharged R2/2 Cu 200 mesh grids (Quantifoil) pre-floated onto custom-made 2.9 nm continuous carbon film ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.5 μL) were applied to precoated (2 nm) R1.2/1.3 carbon-supported copper grids (Quantifoil), blotted for 4-5 s at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of the cell suspension was placed on freshly glow-discharged (for ∼25 s) holey carbon grids (Quantifoil Cu R2/1, 200 mesh). The grids were front blotted with Whatman filter paper and rapidly frozen in liquid ethane ...
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bioRxiv - Biophysics 2020Quote: Samples were prepared for CryoEM by applying 5 μl freshly purified Cx46/50-lipid nanodisc complex (∼2.5 mg mL-1) to a glow-discharged holey carbon grid (Quantifoil R 1.2/1.3, 400 mesh) for 10 seconds ...
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bioRxiv - Bioengineering 2023Quote: ... 3.0 µl aliquots at a concentration of 0.87 or 1.0 mg/ml of the spikeD614G-binder sample or the spikeOmicron-binder sample were applied onto glow-discharged carbon-coated copper grids (Quantifoil R2/1, 400 mesh), blotted for 4.0-8.0 s ...
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Cryo-EM structure of a eukaryotic zinc transporter at a low pH suggests its Zn2+-releasing mechanismbioRxiv - Biochemistry 2022Quote: ... and 3 μl of the protein solution was applied to glow-discharged (45 s) Quantifoil R2/1 300-mesh gold grids (Quantifoil Micro Tools GmbH, Germany). The grids were blotted with standard Vitrobot filter paper for 2.5 s at 4 °C under 100% humidity and plunged into liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 μl of protein at a concentration of approximately 10 mg ml−1 were loaded onto glow-discharged holey carbon grids (Quantifoil Au 300 mesh R1.2/1.3 or Quantifoil Cu/Rh 300 mesh R1.2/1.3), after which ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of ∼100 nM (A260 4.0 or 6.0) mitoribosome sample was applied onto a glow-discharged (20 mA for 30 sec) holey-carbon grid (Quantifoil R2/1 copper, mesh 300) coated with continuous carbon (of ∼3 nm thickness ...
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bioRxiv - Bioengineering 2024Quote: ... LCNP or EV suspensions in PBS (∼1 × 1012 particles/mL) were deposited on 300-mesh lacey carbon-coated copper grids (Quantifoil Micro Tools GmbH, Germany). The grids were previously glow-discharged for 45 s using an Emitech K100X glow discharge system (Quorum Technologies Ltd. ...
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bioRxiv - Cell Biology 2024Quote: ... 4 μl of the cell suspension was applied onto a glow-discharged holey SiO2 grid (R1/4, Au, 200 mesh, Quantifoil) mounted on in the plunger chamber ...
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bioRxiv - Biochemistry 2023Quote: ... 4 μL of Fcμ–J–CD5L was applied to glow-discharged holy-carbon gold grids (Quantifoil, R1.2/1.3, 300 mesh), blotted with filter paper in 100% humidity at 4 °C ...
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bioRxiv - Biophysics 2022Quote: 3.5 μl of protein samples at 4-6 mg/ml were applied to glow-discharged QF R1.2/1.3 300 mesh gold grids (Quantifoil, Großlöbichau, Germany). Grids were blotted for 3 s at 4°C and 100% humidity and plunge-frozen into liquid ethane using FEI Mark IV Vitrobot (FEI ...
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bioRxiv - Plant Biology 2022Quote: ... then 4 µl of the sample was applied to glow-discharged holey carbon grids (Quantifoil Cu R1.2/1.3, 300 mesh). The grid was blotted for 4s and then plunged into liquid ethane using a Vitrobot Mark IV (FEI ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Microbiology 2021Quote: ... 3μl was added to a glow-discharged R2/2 200 mesh holey carbon EM grid (Quantifoil) for 30 seconds and the sample was blotted for 1 second ...
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bioRxiv - Cell Biology 2022Quote: ... A volume of 2.5 μL was applied on glow-discharged UltrAuFoil Au200 R2/2 grids (Quantifoil), and excess liquid was removed by blotting for 3 s (blot force 5 ...