Labshake search
Citations for Quantifoil Micro Tools :
251 - 300 of 784 citations for 4 1 1 2 3 3 3 Hexafluoropropoxy acetophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... trehalose solution to mitigate crystalline ice formation and 4 μL of this mixture immediately applied to a R2/1 Cu 200 grid (Quantifoil), which had been glow-discharged for 1 min at 0.19 mbar and 20 mA in a PELCO easiGlow device shortly before use ...
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bioRxiv - Biophysics 2023Quote: ... 4 µL fresh sample (0.48 mg/mL) was applied on glow-discharged Au holey carbon grids (Quantifoil R 0.6/1) and the following operation were the same as describe above for preparing the sample of gMCM8/9 complex.
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bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
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bioRxiv - Biophysics 2024Quote: ... Microtubules (4 µl drop of 40 µM dilution) were placed onto a glow-discharged holey carbon grid (R2/1; Quantifoil) prepared on a JEC-3000FC (JEOL) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 4 µL of cell suspension (1500–2000 cells/µL) were applied to holey carbon R2/1 or R1/4 copper TEM grids (Quantifoil), and cells were plunge frozen on a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 3.5 μL was applied to a glow-discharged Quantifoil copper grid with 2 nm continuous carbon support over holes (Quantifoil R 2/4 400 mesh), blotted ...
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bioRxiv - Molecular Biology 2021Quote: ... cells in suspension (4 μl of ~1,000 cells per μl) were blotted onto R2/1 carbon-coated 200-mesh copper grids (Quantifoil Micro Tools) and plunge frozen in a liquid ethane/propane mixture ...
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bioRxiv - Biophysics 2020Quote: Three microliters of purified HCoV-NL63 virions (4.5 mg/mL) were first applied to 300-mesh R2/1 + 2 nm C-film grids (Quantifoil) that had been glow-discharged for 15s PELCO easiGlow (TED PELLA ...
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bioRxiv - Microbiology 2021Quote: ... and 5 µl of the mixture was placed onto freshly glow discharged holey carbon grids (R 2/1, Quantifoil) for 1 min ...
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bioRxiv - Biophysics 2023Quote: Gold 200 mesh carbon or SiO2 R 2/1 grids or R 1.2/20 grids (Quantifoil Micro Tools GmbH) were coated with ∼8 nm of carbon using a Leica ACE600 (Leica Microsystems GmbH) ...
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bioRxiv - Biophysics 2024Quote: ... Aliquots of 1.25 – 1.5 ul RyR1-nanodisc were applied onto each side of glow-discharged C-Flat - 2/1 - 3Au holey-carbon (Protochips, NC) or UltraAufoil -1.2/1.3 holey-gold (Quantifoil, Germany) with 300 TEM mesh ...
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bioRxiv - Cell Biology 2024Quote: EM support grids (Au grids 200 mesh, carbon or SiO2 foil, R2/2 or R1/4, Quantifoil) were glow discharged with a Pelco easiGlow for 90 sec at 15 mA ...
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bioRxiv - Molecular Biology 2024Quote: ... 3 µL of the PURE system reaction solution containing stalled ribosomes was applied onto a glow-discharged holey-carbon grid coated with continuous 2 nm-thick carbon film (Quantifoil Au 300 mesh, R2/1 + 2 nm C) and incubated for 30 s in a controlled environment of 100% humidity at 4 ℃ ...
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bioRxiv - Cell Biology 2024Quote: ... the sample was gently resuspended and 4 µl was transferred to a glow-discharged holey carbon R2/1 copper grid (Quantifoil Micro Tools) suspended in the chamber of a Vitrobot (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 5 seconds using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... 3 µL of the concentrated RdRp-RNA complex were mixed with 0.5 µl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). The grids were blotted for 7 seconds with blot force 5 using a Vitrobot MarkIV (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of the concentrated RdRp-RNA complex were mixed with 0.5 μl of octyl ß-D-glucopyranoside (0.003% final concentration) and applied to freshly glow discharged R 2/1 holey carbon grids (Quantifoil). Prior to flash freezing in liquid ethane ...
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bioRxiv - Molecular Biology 2020Quote: ... a 2.2-µL aliquot of this sample was applied to a plasma cleaned holey carbon grid (R2/1, 200 mesh; Quantifoil) or Graphene Oxide-Lacey Carbon grid (300 mesh ...
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bioRxiv - Biophysics 2020Quote: ... Mycoplasma pneumoniae strain M129(ATCC 29342)cells were grown on 200 mesh gold grids coated with a holey carbon support (R 2/1, Quantifoil). Cells were cultivated at 37 °C in modified Hayflick medium ...
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bioRxiv - Cell Biology 2023Quote: Cells were blotted on EM grids (R 2/1 holey carbon 200 films on Cu 200 mesh, Quantifoil Micro Tools). The grids were first glow-discharged and 4 µl of trypanosome wild-type culture (2-4 · 107 cells/ml ...
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bioRxiv - Microbiology 2023Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically backside blotted for 4-6s in a Mark IV Vitrobot (Thermo Fischer Scientific ...
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bioRxiv - Biophysics 2023Quote: ... HeLa cells were trypsinized and seeded at a density of 0.5 – 0.7 X 105 cells/mL on carbon-coated gold Quantifoil grid (200 mesh, R 2/1, holey SiO2 film; Quantifoil Micro Tools GmbH ...
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bioRxiv - Microbiology 2022Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, Quantifoil). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 10 nm protein A-coated colloidal gold (Aurion) and applied onto a glow-discharged EM grid (200 mesh, R 2/1, Quantifoil) prior to plunge-freezing with a Leica EM GP2 automatic plunge-freezer.
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bioRxiv - Microbiology 2024Quote: ... and 4 µl of the mixture was applied to a glow-discharged holey-carbon copper EM grid (R2/1 or R2/2, [Quantifoil]). The grid was automatically blotted from the backside for 4-6 s in a Mark IV Vitrobot by using a Teflon sheet on the front pad ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of the concentrated cells were then spotted onto a glow discharged copper grid (R2/2, Quantifoil). By using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... a 4 µl sample of purified complex was applied to glow-discharged R2/1 300 mesh holey carbon copper grids (Quantifoil Micro Tools GmbH), using a conc ...
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bioRxiv - Cell Biology 2023Quote: 4 ul of Nannochloropsis suspensions (4 x 107 cells mL−1) were pipetted onto a 300 mesh gold Quantifoil grid with 2um holes (Quantifoil Micro Tools, GmbH) and manually blotted from the opposite side in a Vitrobot Mark IV (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 4 µl of this mixture was applied on a glow-discharged Quantifoil R2/2 grid (Quantifoil Micro Tools GmbH) and flash-frozen in liquid ethane using a Vitrobot Mark II device (FEI) ...
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bioRxiv - Microbiology 2024Quote: ... 4 μL of the cell mixture was applied three times onto glow-discharged copper EM grids (R2/2, Quantifoil), followed by plunge freezing into liquid ethane/propane ...
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bioRxiv - Biophysics 2024Quote: ... 4 µL of resuspended solution was applied to freshly glow-discharged Quantifoil R2/2 mesh 300 gold grids (Quantifoil) in a Vitrobot IV (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: 4 µl aliquots of purified samples at A280 = 1 were applied onto glow-discharged holey carbon-coated grids (Quantifoil 300 mesh, Au R1.2/1.3), then adsorbed for 10 s ...
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bioRxiv - Bioengineering 2022Quote: ... holey carbon grids (Quantifoil R2/1 grid: Cu 300mesh, M2951C-1-300) were hydrophilized by a JEC-3000FC Auto Fine Coater (JEOL ...
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bioRxiv - Synthetic Biology 2021Quote: ... 25 mM Tris at pH 8.0 to a concentration of 2 mg/mL to a glow-discharged (1 min at 5 mA) holey carbon grid (Quantifoil Q225-CR1.3). This was followed by plunge-freezing in liquid ethane using an FEI Vitribot Mark IV (100% humidity ...
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bioRxiv - Cell Biology 2020Quote: ... 3.5 µL of gel filtration column eluate (∼0.5 mg/ml) was applied to a holey carbon support (Quantifoil 2/1-4Cu-50) at ∼90% relative humidity ...
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bioRxiv - Cell Biology 2021Quote: ... Glow-discharged holey Quantifoil grids with an additional layer of continuous carbon (R2/1 + 2 nm C, Cu 200 mesh grid, Quantifoil Micro Tools) were used ...
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bioRxiv - Microbiology 2020Quote: ... 3.5 μl of cell suspension from 80-100 μl of final volume were applied on glow discharged 200-mesh EM copper grids coated with R 2/1 holey SiO2 films (Quantifoil Micro Tool GmbH) and plunge frozen in liquid ethane at ~−184°C using a Leica EM GP grid plunger ...
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bioRxiv - Biophysics 2020Quote: ... 3.5ul of cell suspension were applied on 200-mesh copper grids coated with R 2/1 holey carbon or SiO2 films (Quantifoil Micro Tools GmbH) and plunge frozen in liquid ethane at ~-186°C using a Leica EM GP grid plunger ...
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bioRxiv - Biophysics 2021Quote: To provide extra support for cell growth 5-6 nm of carbon was evaporated onto Quantifoil Au 200 R2/1 or R2/2 grids (Quantifoil Micro Tools GmbH). Grids were then glow discharged and incubated in supplemented RPMI-1640 overnight in the cell incubator ...
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bioRxiv - Microbiology 2023Quote: ... Both TBEV strain Neudoerfl and Hypr samples were vitrified in liquid ethane on holey carbon coated copper grids (Quantifoil 2/1, mesh 300, Quantifoil Micro Tools GmbH) using Vitrobot Mark IV (Thermo Fisher) ...
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bioRxiv - Biophysics 2023Quote: Three microliters of the purified GLV-HP or GLV-CAT sample was placed on a glow-discharged holey carbon grid (Quantifoil R2/2 or R2/1, Cu 300 mesh; Quantifoil Micro Tools GmbH) for 3 s blotting time at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 5 µL of the mixture was placed onto freshly glow discharged holey carbon grids (Quantifoil™ R 2/1 on 200 copper mesh) for 1 minute ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of sample were frozen in liquid ethane on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh) using Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Biophysics 2024Quote: ... 4 μl sample was applied to each glow-discharged (30 s at 15 mA) QuantiFoil 2/1 Cu 300 grid (Quantifoil Micro Tools GmbH), pre-coated with graphene oxide according to Boland et al (21) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cu R2/1 grids (Quantifoil) were plasma cleaned using a NanoClean plasma cleaner (Fischione Instruments ...
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bioRxiv - Microbiology 2024Quote: ... cupper R2/1 grids (Quantifoil) were glow discharged ...
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bioRxiv - Molecular Biology 2020Quote: ... plain grids (Quantifoil 2/2) were transferred to the microscope ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µl were frozen in liquid ethane with the Vitrobot Mark IV (FEI company) on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh). Freezing was carried out with Whatman #1 filter papers at 4°C with a blot force of 0 ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: A 4 μL solution of co-isolated small particles were applied to glow-discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools), blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems ...