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Citations for G-Biosciences :
201 - 250 of 321 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Cytotoxicity was quantified colorimetrically (G-biosciences Cytoscan™-LDH Cytotoxicity Assay) wherein LDH activity is measured by recording A490 after 20 min incubation with substrate mix at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Cleared lysates were passed through a 5 ml gravity column (G-Biosciences, MO, USA) containing 1.5 ml of Ni2+-NTA agarose resin (ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... CB-Protein Assay kit and MCP-1 ELISA kit from G-Biosciences, India ...
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bioRxiv - Plant Biology 2022Quote: ... after inducing expression with 1 mM IPTG for 16 h at 20°C and purified by cobalt-nitrilotriacetic acid affinity chromatography according to the manufacturer’s instructions (G-Biosciences), including a washing step with 5 mM Mg-ATP ...
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bioRxiv - Bioengineering 2022Quote: Purification of tagged-GRFT was achieved by using a zinc-chelating resin (binding capacity 20mg/mL) (G-Biosciences). A calculated amount of resin was added to a 0.8 mL Pierce centrifuge spin column based on the estimated total protein yield obtained by Western blot analysis ...
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bioRxiv - Genomics 2022Quote: Genomic DNA was extracted from 30 mg of urediniospores per isolate using the Omniprep DNA isolation kit (G-Biosciences). DNA concentration was determined using Qubit 3.0 Fluorometer (LifeTechnologies ...
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bioRxiv - Synthetic Biology 2021Quote: ... Protein sample was then transferred to midi-size 1 kDa MWCO Tube-O-Dialyzers (G-Biosciences) and first dialyzed against 100 volumes of simplified high-salt buffer with urea overnight ...
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bioRxiv - Microbiology 2021Quote: ... Iodoacetamide (G-Biosciences, St. Louis, MO, or Sigma-Aldrich) was prepared as a 10 mM stock solution in HyPure H2O (Cytiva Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... the cells were induced with IPTG overnight at 18°C followed by sonication and purification using Ni-NTA resin (Cat. No # 786-940, G-Biosciences, USA). The purity was confirmed by SDS-PAGE and the quantity was measured using NanoDrop 2000 (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and the peptides were de-salted on a C18 spin column (G-Biosciences #786-930) and dried in a vacuum concentrator.
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an Omniprep kit for genomic DNA (G-Biosciences, St. Louis, MO, USA), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was extracted using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO), following the protocol of Espeland et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We assessed overall protein concentration in the upper fraction of the solution using RED 660™ protein assay (G-Biosciences) measured in triplicates and then diluted the sample to 0.45 mg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... were reacted for 2 h with 150 μL/cm2 of a 3 mg/mL suspension of sulfo-succinimidyl-4-(p-maleimidophenyl)-butyrate (S-SMPB, #BC24, G-Biosciences) in phosphate buffered saline solution (PBS ...
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bioRxiv - Immunology 2021Quote: ... 1:1 NAP-Blocker (NAP [Non Animal Protein]-BLOCKER™, G-Biosciences cat # 786-190) in TBS was added for 1 hour at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Biochemistry 2021Quote: ... the TtArsM arsenite methyltransferase activity was measured using the SAM510: SAM Methyltransferase Assay Kit (G-Biosciences) with modifications regarding the temperature ...
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bioRxiv - Bioengineering 2021Quote: ... polymerized gels were conjugated with sulfo-SANPAH (G-Bioscience) in HEPES buffer (pH = 8.5 ...
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bioRxiv - Biophysics 2021Quote: ... the supernatant was affinity purified using Protein A affinity resin (G-Biosciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... amplicons were loaded in a 1% low EEO agarose gel (G-BioSciences) and purified using the QIAquick Gel Extraction Kit (QIAGEN ...
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bioRxiv - Neuroscience 2021Quote: ... Amplicons were loaded into the agarose gel (G-BioSciences) and visualized using Gel-Doc XR+ Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... and protein visualised with Ponceau Stain (G-Biosciences). Membranes were blocked in 5% BSA TBS-T (5% BSA in 1x Tris Buffered Saline ...
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bioRxiv - Physiology 2021Quote: ... gels were rinsed in diH2O for 15 minutes and immersed in Coomassie stain (Lab Safe GEL Blue; G-Biosciences; St. Louis, MO, USA) for 2 hours ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were then sonicated and cell lysate containing GST tagged anti-GFP nanobody was incubated with glutathione resin (G-Biosciences, U.S.A.) at 4°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... and protein concentration was determined using a noninterfering protein assay (G-Biosciences). 12 μg of protein was resolved by SDS-PAGE on a 17.5% acrylamide gel and transferred to an Immobilon-P membrane (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... Samples were dialyzed against water (1 kDa MWCO, G-Biosciences Tube-O-DIALYZER) and stored at -80 deg C prior to inositol quantification by the UCSD GlycoAnalytics Core.
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bioRxiv - Microbiology 2021Quote: ... Proteins were purified by cobalt-charged resin chromatography (G-Biosciences).
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bioRxiv - Plant Biology 2021Quote: ... The proteins were assayed using the CBX kit (G-Bioscience). An aliquot of proteins was reduced with DTT and alkylated with iodoacetamide prior to digestion with LysC and then trypsin ...
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bioRxiv - Microbiology 2021Quote: ... 294 µL of 10X Tris-EDTA buffer pH 8 (G-Biosciences, Cat. no. 501035446) was added to the Lysing Matrix E tube and supplemented with 6 µL of lysozyme (50 mg mL-1 ...
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bioRxiv - Molecular Biology 2021Quote: ... the supernatant was affinity purified with Ni-NTA agarose bead (G-Biosciences) and eluted into PBS containing 250 mM imidazole and further concentrated and desalted with an Amicon Ultra-15 Centrifugal Filter Device (Merck) ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads were washed three times in 1X TESH buffer with 0.15 M NaCl and resuspended in 40 µl of RIPA buffer (G-Biosciences, MO) on ice for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... or Non-Interfering protein assay (G-Biosciences), and sample concentrations were normalized by addition of Laemmli sample buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration of the samples was measured by Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572). 2-mercaptoethanol was added to a final concentration of 1% (v/v) ...
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bioRxiv - Cell Biology 2021Quote: ... Total protein concentration in BALF was determined using a Micro Bicinchoninic Acid Protein Assay kit (G-Biosciences; #786-572).
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bioRxiv - Cancer Biology 2021Quote: ... 1mM CaCl2 (G-Biosciences, Cat# R040), 21mM MgCl2 (G-Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... 21mM MgCl2 (G-Biosciences, Cat# R004), 0.05% BSA (EMD Millipore ...
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bioRxiv - Plant Biology 2021Quote: ... was transformed using the Fast Yeast Transformation kit (G-Biosciences, MO, USA). All transformants were selected on a yeast minimal medium without tryptophan ...
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bioRxiv - Microbiology 2021Quote: ... monolayers were overlaid with 2 ml of a solution consisting of equal parts of 1.4% (w/v) Low Melt Agarose (G-BioSciences) and 2X MEM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatographic columns were filled with 2.5 mL Nickel-Chelating-Resin 50 % slurry in 20 % ethanol (G-Bioscience), washed with 10 column volumes of lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... AceTau (1:500, #ITK0084, G-Biosciences), MC1 (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... the bead pellets transferred to a 1ml spin column (G-Biosciences, 786-811) and washed once more with 1 bead volume of 1x binding buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1 mM Phenylmethylsulfonyl fluoride (PMSF) (786-055, G-Biosciences) for 10 min on ice ...
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bioRxiv - Plant Biology 2021Quote: ... The VirE2 protein was detected by anti-VirE2 polyclonal primary antibody (G-Biosciences, 1:10 000) and anti-rabbit secondary antibody (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... The VirE2 protein was detected by the anti-VirE2 polyclonal antibody (G-Biosciences, 1:10000) and anti-rabbit secondary antibody (Sigma ...
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bioRxiv - Genomics 2021Quote: ... and pellets frozen at -80°C. DNA was extracted from pellets (approx. 100 mg) using an OmniPrep kit (G-Biosciences, St. Louis, MO) in 1 ml lysis buffer (G-Biosciences ...
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bioRxiv - Genomics 2021Quote: ... in 1 ml lysis buffer (G-Biosciences) with 10 µl proteinase K and digested for 1 hour at 60°C with periodic mixing to keep spores suspended in the lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... Digestion was done using proteomics grade trypsin 1:20 (G-Biosciences, St. Louis, MO) and a CEM Discover Microwave Reactor (Mathews ...
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bioRxiv - Biochemistry 2021Quote: ... The detergent present in the incubation mixture with BPS was removed using a DetergentOUT™ spin column (G-Biosciences, St Louis, MO). The protein was concentrated to 25-30 µM with the use of a Centrisart I MWCO 100 kDa concentrator (Sartorius AG ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 mM Tris-HCl pH 8) supplemented with PhosphataseArrest (G-Biosciences, Saint-Louis, MO, USA) and protease inhibitors (Complete Mini® ...
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bioRxiv - Immunology 2021Quote: 2μg/mL of Streptavidin (G-Biosciences) was coated onto each wells of Nunc maxisorp high protein-binding 96 well ELISA plate (Thermo Fisher Scientific Inc. ...