Labshake search
Citations for G-Biosciences :
1 - 50 of 321 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... magnetic silica beads (MGSBs, G-Biosciences, St. Louis, MO) were used to separate microsomal protein (Horspool et al. ...
-
bioRxiv - Microbiology 2024Quote: ... 1% bromophenol blue (G-Biosciences)) ...
-
bioRxiv - Bioengineering 2024Quote: ... AlamarBlue (cat 786-921) was purchased from G-Biosciences. ActinRed 555 ReadyProbes reagent (R37112 ...
-
bioRxiv - Plant Biology 2024Quote: ... All primers were designed using the tool Primer Quest and purchased from G-Biosciences. All PCR reactions were performed using the PCR components from Takara bio Inc ...
-
bioRxiv - Molecular Biology 2024Quote: ... For the SAMfluoro methylation assay (G-Biosciences), FLAG-METTL3 variants were immobilized on 25 µL antibody-conjugated protein G dynabeads (Invitrogen ...
-
bioRxiv - Microbiology 2024Quote: ... genomic DNA was extracted using the Omniprep DNA isolation kit (G-Biosciences, St. Louis, MO, USA) from 20 mg of urediniospores of Pca isolate NTU1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, St. Louis, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 × g for 10 min) ...
-
bioRxiv - Microbiology 2024Quote: ... yeast transformation kit from G-Biosciences (USA), trizol reagent and DMEM from Gibco (USA) ...
-
bioRxiv - Genomics 2024Quote: ... Total protein staining was performed using Swift protein stain (G-Biosciences). Membranes were blocked in 5% milk in TBS- Tween for 1h prior to incubation with α-GFP (1:1000 ...
-
bioRxiv - Bioengineering 2024Quote: ... The construct was then dissolved in 200 µL formamide (G-Biosciences) for 48 h at room temperature to recover the dye ...
-
bioRxiv - Cancer Biology 2024Quote: ... Stock solutions for drugs were prepared in 100% sterile DMSO (G-Biosciences cat# 786-1388).
-
bioRxiv - Microbiology 2024Quote: ... The Cobalt Chelating Resin (G-Biosciences) was washed with water and preequilibrated with binding buffer ...
-
bioRxiv - Immunology 2024Quote: ... One hundred fifty µL blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... The reaction was allowed to proceed for 20 min at 56°C and alkylated for 30 min in the dark with 10 mM iodoacetamide at room temperature (G-Biosciences, St. Louis, MO, USA). Proteins were precipitated at –20°C with ethanol ...
-
bioRxiv - Biochemistry 2024Quote: ... the supernatant was purified with Ni-NTA affinity agarose beads (G-Biosciences) on a column (Thermo Fisher ScientificTM PierceTM Centrifuge Columns 10 mL) ...
-
bioRxiv - Bioengineering 2024Quote: ... after which Ni-NTA Resin (G-Biosciences, St. Louis, MO) was added to the solution ...
-
bioRxiv - Cancer Biology 2024Quote: ... protein concentrations measured using the Red 660 Protein Assay (G-Biosciences; St. Louis, MO), then lysate concentrations were normalized ...
-
bioRxiv - Molecular Biology 2024Quote: ... The cells were lysed with Nonidet P-40 (NP40) buffer supplemented with PIC (Cat: 786-437, G-Bioscience). Importin-ß was precipitated by protein A/G beads conjugated with anti-Importinβ-1 or anti-p24 antibody while incubating for 12 h in 4°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Optical density measurements were normalized against a SWIFT Membrane total protein stain (G-Biosciences), and subsequently presented as a percentage relative to the naive condition.
-
bioRxiv - Bioengineering 2024Quote: ... 5 g/L yeast extract (Cat #RC-117, G-BIOSCIENCES), and 10 g/L NaCl (Cat # BDH9286 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Washed beads were transferred to spin columns (G-Biosciences) and centrifuged (100 x g ...
-
bioRxiv - Biochemistry 2024Quote: ... then transferred the resin suspension to a 1 mL spin column (G-Biosciences). We collected msGFP-bound resin be spinning at 1000rcf ...
-
bioRxiv - Immunology 2024Quote: dHL60 cells were lysed in RIPA buffer (G-Biosciences # 786-489) on ice by sonication with 3 sec ON/OFF cycles for 10 cycles ...
-
bioRxiv - Immunology 2024Quote: ... as detected by Swift Membrane Stain (G-Biosciences).
-
bioRxiv - Biochemistry 2024Quote: ... Separated proteins were visualized by staining with Coomassie Blue R250 (G-Biosciences).
-
bioRxiv - Biochemistry 2024Quote: ... The gel was fixed in 50% ethanol and 10% acetic acid for 30min and was stained with Coomassie Blue R250 (G-Biosciences). The portions of the gel containing the samples were excised and the gel pieces were subjected to in-gel trypsin digestion [30].
-
bioRxiv - Biochemistry 2024Quote: ... and the supernatant dialyzed against the resuspension buffer in a mini tube-O-dialyzer (G-Biosciences) with a cut-off of 1 kDa ...
-
bioRxiv - Biochemistry 2024Quote: ... 6 M Urea) and dialyzed against 100 volume TP70 Urea buffer in Tube-O-Dialyzer™(G-Biosciences) at 4 °C overnight and then dialyzed against 100 volume TP70 buffer (TP70 Urea buffer without Urea ...
-
bioRxiv - Systems Biology 2024Quote: ... Supernatant lysate was collected and SmEn was purified in the gravity flow mode using Ni-NTA resin (G-bioscience, Cat. # 786-1547) following the manufacturer’s recommended procedure with slight modifications ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... stored in an envelope in a -80 freezer since collection) and dried, pinned specimens (i.e., traditional museum preservation method) using an OmniPrep Genomic DNA Extraction Kit (G-Biosciences, St. Louis, MO) and evaluated DNA quality using agarose gel electrophoresis and quantity using Qubit 2.0 fluorometer (ThermoFisher Scientific) ...
-
bioRxiv - Plant Biology 2024Quote: ... induced at 22°C for 12h with 1mM IPTG was immobilized into glutathione Resin (G-Bioscience), as described by Banerjee et al. ...
-
bioRxiv - Plant Biology 2024Quote: ... yeast-competent cells of Y2H GOLD strain were prepared according to manufacturer’s instructions (Fast™ Yeast Transformation, G-Biosciences). The two respective constructs were co-transformed in Y2H GOLD competent cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... After one hour of blocking in membrane blocking agent (G-Biosciences, Cat-786-011), incubation with primary antibodies was done overnight at 4°C ...
-
bioRxiv - Genomics 2024Quote: High molecule weight (HMW) DNA of Ectocarpus male (Ec32) and female (Ec25) were isolated using OmniPrep™ kit (G-Biosciences) with slight modifications ...
-
bioRxiv - Physiology 2024Quote: ... Peptides were then purified and desalted using C18 spin columns (G-Biosciences), speed-vacuumed dry ...
-
bioRxiv - Biochemistry 2024Quote: Colonies showing evidence of both LacZ and His3 expression were cultured and lysed using Zymolyase solution (50:49:1, glycerol:Zymolyase [G-Biosciences]: 1M Tris) and prey plasmids were purified by alkaline lysis miniprep (Joly ...
-
bioRxiv - Biophysics 2024Quote: ... DSS and EDC (G-Biosciences), were dissolved in DMSO at stock concentrations of 12.5 mM and 120 mM ...
-
bioRxiv - Plant Biology 2024Quote: ... GST-fused protein or GST (control) was bound to glutathione agarose beads (G-biosciences®; USA), overnight ...
-
bioRxiv - Microbiology 2024Quote: ... Samples (990 µl) were then treated with 10 µl of protease inhibitor cocktail FOCUS ProteaseArrest (G-Biosciences) and kept on ice ...
-
bioRxiv - Immunology 2024Quote: ... protein was digested with trypsin (G-Biosciences, St. Louis, MO) at a 1:50 ratio of trypsin/protein ...
-
bioRxiv - Plant Biology 2024Quote: ... MBP-fused proteins were purified using MBP resin and the GST-tagged proteins were purified with glutathione agarose beads (G-biosciences®; USA). For in vitro pull-down assays ...
-
bioRxiv - Plant Biology 2024Quote: ... MBP-fused proteins or MBP (control) were bound to MBP resin (G-biosciences®; USA). These were incubated with GST-fused protein and similar steps were performed as mentioned above ...
-
bioRxiv - Molecular Biology 2024Quote: ... All Ulva gene and promoter sequences were cloned from genomic DNA, isolated via CTAB (Clarke, 2009) or OmniprepTM for Plant (G-Biosciences).
-
bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein lysates were denatured in SDS-polyacrylamide gel electrophoresis (PAGE) Sample Loading Buffer (G-Biosciences), separated on 4-12% Bolt Bis-Tris Plus Protein Gels (ThermoFisher) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The beads were washed with 300 μL of His-Binding/Wash Buffer (786-542, G-Biosciences) and phosphate-buffered saline (1X PBS ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was transferred to a new tube and mixed with 2 mL of His-Binding/Wash Buffer (786-542, G-Biosciences) and 5 μL of HisTag Dynabeads (10104D ...
-
bioRxiv - Microbiology 2024Quote: ... yeast transformation kit from G-Biosciences (USA), ER trackerTM Green (BODIPYTM FL Glibenclamide ...
-
bioRxiv - Cell Biology 2024Quote: Mitochondrial ferritin in HCEC-B4G12 and F35T cells were quantified using Immunotag™ Mitochondrial Ferritin ELISA kit (G-Biosciences, USA). Protein was extracted following the same protocol used for the cytosolic ferritin ELISA ...
-
bioRxiv - Neuroscience 2024Quote: ... and 1 mM PMSF (82021, G-Biosciences). The cell lysates were clarified by centrifugation at 13,000 g at 4°C for 10 minutes ...
-
bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% milk (G-Biosciences 786-011) for 1 hr and incubated in primary antibodies overnight at 4 °C ...