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Citations for G-Biosciences :
51 - 100 of 321 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... The supernatant was transferred to a new tube and mixed with 2 mL of His-Binding/Wash Buffer (786-542, G-Biosciences) and 5 μL of HisTag Dynabeads (10104D ...
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bioRxiv - Molecular Biology 2024Quote: ... All Ulva gene and promoter sequences were cloned from genomic DNA, isolated via CTAB (Clarke, 2009) or OmniprepTM for Plant (G-Biosciences).
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein lysates were denatured in SDS-polyacrylamide gel electrophoresis (PAGE) Sample Loading Buffer (G-Biosciences), separated on 4-12% Bolt Bis-Tris Plus Protein Gels (ThermoFisher) ...
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bioRxiv - Biochemistry 2024Quote: ... equal loading was assessed using BlotFastStain (G-Biosciences, 786-34), then membranes were blocked in 5% NFDM in TBST overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: We added 200 ng in 1 µL of plasmid suspended in IDTE (76286-184, G-Biosciences) to the 40 µL of competent cells ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was blocked with 5% blocking powder (G-Biosciences 786-011) to prevent nonspecific binding and incubated with the appropriate primary antibodies overnight at 4°C.
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bioRxiv - Cancer Biology 2024Quote: Proteins were extracted by suspending cell pellets in RIPA Lysis Buffer (G-Biosciences, 786-489) supplemented with 1× Protease Inhibitor Cocktail (Thermo ...
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bioRxiv - Cancer Biology 2024Quote: ... the membranes were visualized using the FemtoLucent Plus HRP detection system (G-Biosciences, 786-003), and protein bands were imaged for analysis.
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bioRxiv - Bioengineering 2024Quote: ... Tween-20 (10%) was purchased from G-Biosciences (MO, USA). Paraformaldehyde (4% ...
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bioRxiv - Biophysics 2024Quote: ... biotin was conjugated to the λ-phage DNA using either photobiotin (PHOTOPROBE®, Vector) or psoralen-biotin (HOOK™-Psoralen-PEO-Biotin, G-biosciences). The biotin reagent solution was added to the purified λ-phage DNA solution in a 50:1 volume ratio for photobiotin and a 100:1 for psoralen-biotin ...
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bioRxiv - Cancer Biology 2024Quote: ... with SDS Neutralizer (G-Biosciences, Overland, MO) prior to SDS-PAGE and transfer to Immobilon-FL membranes (Millipore Sigma ...
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bioRxiv - Physiology 2024Quote: ... with protease and phosphatase inhibitor cocktails (97063-010, VWR and 786-450, G-Biosciences, St, Louis, MO, USA) using a THb Handheld Tissue Homogenizer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extraction was performed using the OmniprepTM for Plant DNA extraction kit (G-Biosciences), and the extracted DNA was diluted to a final concentration of 0.1 ng.μL-1 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein concentration of the samples was determined using the CB-X Protein assay (G-Biosciences) according to manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in the RIPA buffer according to their size (G-Biosciences-786-490). Protein concentrations of total lysates of WT and Mgat4b KO cells were estimated by the Bradford assay (Da Silva & Arruda ...
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bioRxiv - Biochemistry 2024Quote: ... then changed to developing buffer (G-Biosciences, St. Louis, MO) for an additional 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Conditioned media was collected incubated with Concanavalin A Agarose beads (G-Biosciences, Overland, MO) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Calmodulin resin (G-Biosciences) was prewashed three times with EDTA-free lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... as the supplier (G-biosciences, Protease Inhibitor Set ...
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bioRxiv - Biochemistry 2024Quote: ... and 2 mL of 10% neutral buffered formalin (G-Biosciences, 786-1056) was added to cells for 60 min ...
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bioRxiv - Bioengineering 2024Quote: ... The resultant post-insertion mixture was purified using Sepharose CL-6B gel filtration columns (G-Biosciences, 76361-752).
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bioRxiv - Cell Biology 2024Quote: Isolated brush border was homogenized at 4 °C for 30min in RIPA lysis buffer (G-Biosciences catalog #786-489) with cOmplete Protease Inhibitor Cocktail followed by centrifugation at 14,000g for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... The gels were submerged under 1mg/ml sulfo-SANPAH (G-Biosciences, St. Louis, MO) solution and placed 2 inches below an 8 W ultraviolet UV lamp (Hitachi F8T5 – 365nm ...
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bioRxiv - Plant Biology 2023Quote: The purification of recombinant protein was carried out on a Ni-NTA column (G-Biosciences, USA). One ml of Ni-NTA Sefinose resin was loaded onto a plastic column ...
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bioRxiv - Neuroscience 2023Quote: ... lysed in RIPA buffer (G-Biosciences) supplemented with 1x Halt protease inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... loaded on Ni-NTA 0.2ml column (G-BIOSCIENCES), and DHFR proteins were isolated using manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... by homogenizing the tissue with a 2 cm3 glass-Teflon homogenizer at 800 rpm in lysis buffer (0.32 M sucrose, 5 mM Hepes, and 10 ml ddH2O) supplemented with 1:100 diluted proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 diluted phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Neuroscience 2023Quote: ... pH=7.5) supplemented with 1:100 proteases inhibitors (G-Biosciences, Cat. no: 786-433) and 1:100 phosphatases inhibitors (Sigma-Adrich ...
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bioRxiv - Immunology 2023Quote: ... in 1×Tris-buffered saline Tween-20 (TBST, G-Biosciences, Cat#R042), and analyzed with the indicated primary antibodies (biotinylated anti-H3K27Me3 (1:1000 ...
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bioRxiv - Genetics 2023Quote: ... coli prepared using Z-Competent cell kit (G-Biosciences, St. Louis, MO) and plated to achieve approximately 104 colonies per plate ...
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bioRxiv - Cancer Biology 2023Quote: ... After blocking the membranes with 5% blotto (G-Biosciences Blot-Quikblocker # 786-011) in phosphate-buffered saline containing 0.1% (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... Sp1 (G-Biosciences)].
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bioRxiv - Cancer Biology 2023Quote: ... and 1.6 µL 1X TE Buffer (pH 8.0, Low EDTA (Tris-EDTA; 10 mM Tris base, 0.1 mM EDTA)) (PN 786-150, G-Biosciences, St. Louis, MO). Each dPCR reaction mix was then vortexed three times for five second pulses ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was clarified by centrifugation and incubated with glutathione resin (G-Biosciences cat #786-310) for 1 hour ...
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bioRxiv - Physiology 2023Quote: ... Total protein was measured via BLOT-FastStain (G-Bioscience, St. Louis, MO, USA) and used as a loading control ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked for >60 min at room temperature with gentle shaking in 5% BLOT-QuickBlocker (w/v) (G-Bioscience 786-011) in phosphate buffered saline with 0.05% Tween-20 v/v (PBS-T).
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bioRxiv - Microbiology 2023Quote: ... purified proteins were mixed with alum adjuvant (G-Bioscience, St. Louis, MO) in a 2:1 ratio of antigen to adjuvant ...
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bioRxiv - Physiology 2023Quote: ... TEV protease and any free MBP or uncleaved MBP fusion were further removed using a cobalt resin (Catalog 786-402, G-Biosciences).
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bioRxiv - Plant Biology 2023Quote: ... Protein amounts were quantified using the CB-X™ protein assay (G-Biosciences, St Louis, MO). 1 mg of each sample was reduced at 37°C for 1 h and then alkylated with 20 mM iodoacetamide (20 min at RT in darkness) ...
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bioRxiv - Cell Biology 2023Quote: ... then excised and placed individually in 2-mL centrifuge tubes (G-Biosciences, Cat. #C041-D) for DNA purification.
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bioRxiv - Bioengineering 2023Quote: ... ELISA kits for rabbit TNFα and IL1β were from G-biosciences (St. Louis, MO, USA). A lipid peroxidase assay kit was from Eagle Biosciences (Amherst ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were blocked with 5% BSA in TBST and blotted according to manufacturer’s recommendations for the indicated primary and secondary antibodies (Table S2) followed by development with the femtoLUCENT™ PLUS-HRP Chemiluminescent kit (G-Biosciences) for the anti-GDF3 blot and the SuperSignal™ West Pico Chemiluminescent kit (Thermo Fisher ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was collected and incubated with 3 ml of cobalt-charged resin (786-403, G-Biosciences) for 3 hours at 4℃ ...
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bioRxiv - Pathology 2023Quote: Aortic samples were homogenized in Cell Lysis buffer and aortic protein (10 µg) was dissolved in zymogram sample buffer (786-483, G-Biosciences). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... the protein concentration of each eluate was determined using a non-interfering protein concentration determination kit (G-Biosciences), and each prep was normalized to 1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The cleared lysate was then incubated with glutathione resin (G-Biosciences cat #786-310) for 3 hours ...
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bioRxiv - Immunology 2023Quote: ... the endotoxin contaminant was removed using EndotoxinOUT™ resin from G-Bioscience. The polypropylene column was filled with 4 ml EndotoxinOUT™ resin and washed with 5 bed volumes of 1 % sodium deoxycholate in LAL Reagent Water (LRW) ...
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bioRxiv - Biochemistry 2023Quote: ... Ni-NTA resin (G-Biosciences, St. Louis, MO, USA) was added to the supernatant after clarification by centrifugation at 15 k rpm at 4 °C for 30 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... LongLife Zymolyase was purchased from G-Bioscience, and Endoglycosidase H (Endo H ...
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Nucleocytoplasmic transport rates are regulated by cellular processes that modulate GTP availabilitybioRxiv - Cell Biology 2023Quote: ... the gels coated with Sulfo-SANPAH (G-Biosciences) were functionalized using high intensity UV for 216 s ...