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Citations for G-Biosciences :
101 - 150 of 321 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Fixed cells were lysed in 3X volume of lysis buffer (1% SDS, 10mM EDTA, 50mM Tris-HCl pH 8.0, 1X protease inhibitor cocktail (G-Biosciences, 786-433). Cells were sonicated for 35 minutes using a Diagenode Bioruptor to shear chromatin to 200-500bp ...
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bioRxiv - Cell Biology 2023Quote: ... SYBR Green-Labelled PCR primers for all targeted genes were purchased from G-Biosciences made in (St ...
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bioRxiv - Microbiology 2023Quote: Protein estimation was done using Bicinchoninic Acid (BCA) Protein Assay kit (G-Biosciences). Cell lysate (25ul aliquot of each replicate ...
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bioRxiv - Neuroscience 2023Quote: ... MMP2 was renatured in buffer (G-biosciences) for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... transcripts were detected by gene-specific 32P-labeled oligonucleotides (Supplementary Table S1) in hybridization buffer (G-Biosciences, Saint Louis, MI, USA) and exposed to a phosphor screen overnight ...
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bioRxiv - Biochemistry 2023Quote: ... The gels were submerged under 1mg/ml sulfo-SANPAH (G-Biosciences, St. Louis, MO) solution and placed 2 inches below an 8 W ultraviolet UV lamp (Hitachi F8T5 – 365nm ...
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bioRxiv - Bioengineering 2023Quote: Cytoscan-LDH cytotoxicity assay kit (G-BIOSCIENCES 786-210) was used to measure LDH release from treated spheroids ...
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bioRxiv - Cancer Biology 2023Quote: Whole cell lysates were prepared by dissolving (10 million cells per treatment) cell pellets in RIPA buffer (G-Bioscience,) complete with 1X Protease and Phosphatase inhibitor cocktail (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... frozen tumor tissues were crushed to powder and lysed with urea lysis buffer containing 8 M urea (G-Biosciences #BC89), 75 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... at a ratio of 15:1 (see Supplementary Information, 2. for more details) and 10 µL silica magnetic beads (G-Biosciences, USA). The mixture was briefly vortexed and incubated for 20 minutes at room temperature while shaking at 1000 rpm to facilitate DNA binding ...
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bioRxiv - Neuroscience 2023Quote: ... Gels were then changed to developing buffer (G-Biosciences) for an additional 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration was determined using a Bradford assay (47) with bovine serum albumin (BSA) as the standard (G-Biosciences, Geno technology inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Total protein concentration was determined using a BCA Protein Assay kit (G-Biosciences) and subsequently treated with DNase I (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then transferred to polyvinylidene difluoride (PVDF, G-Biosciences) membranes by wet blotting using standard methods ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was prepared from liquid cultures using the XIT Genomic DNA from Gram-Positive Bacteria kit (G-Biosciences), as instructed by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... Tween-20 (BTNM-0080) was purchased from G-Biosciences (MO, USA).
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bioRxiv - Cell Biology 2023Quote: ... 20µls 50% Glutathione agarose (G-Biosciences) was added ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were then spun for 10 min at 6000 rpm at 4 °C and pre-cleared by incubating them under rotation with 25 μl of Sepharose CL-4B beads (G-Biosciences, #786-1560) for further 30 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... Total protein loading was assessed by BlotFastStain (G-Biosciences). Blots were imaged using Bio-Rad CheiDoc MP imaging system and band density was quantified using ImageJ.
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bioRxiv - Bioengineering 2023Quote: ... Protein concentration of EV samples was determined using a bicinchoninic acid (BCA) assay using the manufacturer’s protocol (785-571; G-Biosciences, St. Louis, MO, USA). Equal amounts of protein of EV or lysate samples were then subjected to western blot analysis for ALIX (ab186429 ...
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bioRxiv - Cell Biology 2023Quote: ... The blots are developed by G-biosciences ECL reagent and chemiluminescence is detected using high-resolution chemiluminescence mode on Bio-Rad chemidoc XRS+ system ...
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bioRxiv - Genomics 2023Quote: ... we followed the kit standard protocol for Omniprep extraction of genomic DNA (G-Biosciences, St. Louis, MO) and sequenced 250 basepair paired-end reads on an Illumina MiSeq.
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bioRxiv - Immunology 2023Quote: ... rabbit anti-COL25A1 (G-Biosciences ITT1021); goat anti-IGSF8 (R&D systems AF3117-SP) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... we assessed protein concentration in triplicate using RED 660™ protein assay (G-Biosciences). Following earlier tapeworm homogenate injection experiments (Vrtílek and Bolnick 2021 ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein loading was assessed by BlotFastStain (G-Biosciences). Blots were imaged using Bio-Rad ChemiDoc MP imaging system and band density was quantified using ImageJ.
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bioRxiv - Biochemistry 2023Quote: ... C-His tagged IDH1 R132H (Met1-Leu414) was purchased from G-Biosciences (BAN1708, 50 µg). N-His and GST tagged USP7 (Lys208-Glu560 ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of the protein was done using Bradford assay (Puregene PG-035-500mL) or BCA assay (G-Biosciences-786570). For western blotting 25 ug protein was loaded in each well ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 mM of sodium azide (G-Biosciences) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... 1 ml of nickel-chelating resin slurry (G-Biosciences) was added to the cleared lysate ...
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bioRxiv - Neuroscience 2023Quote: Media samples were collected from individual hCO wells and run using the Cytoscan LDH Cytotoxicity Assay (G-Biosciences, 786-210) per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration in BEV samples was determined using Bicinchoninic acid assay (BCA; 786-571; G-Biosciences). AEC samples were buffer exchanged with 1× PBS prior to BCA to remove excess NaCl and adjust pH to 7.4.
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bioRxiv - Immunology 2023Quote: ... 150 µl blocking buffer (one-third Non-Animal Protein (NAP)-Blocker (G-Biosciences #786-190P) and two-thirds PBS ...
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bioRxiv - Microbiology 2023Quote: ... CBX™ protein assay kit (G-Bioscience, USA) was utilized for protein quantification ...
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bioRxiv - Molecular Biology 2022Quote: ... Primary antibodies were incubated in 5% BLOT-QuickBlocker (G-Biosciences 786-011) as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Glutathione resin was purchased from G-Biosciences, St ...
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bioRxiv - Biochemistry 2022Quote: ... followed by incubation in developing buffer (30 min at RT, G-Biosciences, St. Louis, MO), and again in fresh developing buffer overnight at 37°C with gentle shaking ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: Cells were harvested and lysed in RIPA buffer (G-Biosciences, CA95029-284) complete with protease/phosphatase inhibitor cocktail (Thermo ...
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bioRxiv - Bioengineering 2022Quote: LDH release was measured with Cytoscan-LDH Cytotoxicity Assay (G-BIOSCIENCES 786-210). Briefly ...
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bioRxiv - Microbiology 2022Quote: His-FhaAMtb was induced with 1mM IPTG at 18°C overnight and purified on Ni-NTA resin (G-Biosciences, 786-940 in 5 ml Bio-Scale Mini Cartridges ...
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bioRxiv - Microbiology 2022Quote: ... and purified on Ni-NTA resin (G-Biosciences 786-940) then dialyzed and concentrated ...
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bioRxiv - Microbiology 2022Quote: ... Particle pellets were resuspended in 1× STE buffer (100 mM NaCl, 10 mM Tris pH 8.0, and 1 mM EDTA) (G-Biosciences, MO). The 293T/17 cells were collected and lysed with RIPA lysis buffer and clarified via centrifugation (1800 x g for 10 min) ...
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Telomerase deficiency in humans is associated with systemic age-related changes in energy metabolismbioRxiv - Cell Biology 2022Quote: ... Protein concentration of the samples was determined using the CB-X Protein assay (G-Biosciences) according manufacture’s guidelines ...
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bioRxiv - Bioengineering 2022Quote: ... Total protein stains were done using Swift Membrane Stain (G-biosciences). The membrane was imaged using a LI-COR Odyssey CLX Imager ...
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bioRxiv - Biochemistry 2022Quote: ... and 1X Bacterial Protease Arrest (G-biosciences) to 150 O.D.600/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing RNase E were loaded onto a G-Sep™ 6-600 kDa Size Exclusion Columns (G-Biosciences) and eluted with storage buffer (20 mM Tris HCl pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions were loaded onto a G-Sep™ 6-600 kDa Size Exclusion Columns (G-Biosciences) and eluted with storage buffer (20 mM Tris HCl pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... Roche/Sigma-Aldrich)] and protein concentration was determined using CB-X Protein Assay kit (G-Bioscience, St. Louis, MO). After trypsination ...
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bioRxiv - Cancer Biology 2022Quote: The Luc-Ce6 conjugate was synthesized by directly conjugating Renilla luciferase 8 (Rluc8, or simply Luc, G-Biosciences) with chlorin e6 mono 6-amino hexanoic acid amide (Ce6-HA ...
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bioRxiv - Immunology 2022Quote: ... 1:1 Non Animal Protein-BLOCKER™ (G-Biosciences) in TBS was added for 1 hour at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The soluble recombinant E protein was recovered by 6-HIS affinity chromatography on Ni-NTA agarose (G-Biosciences) and purified by size exclusion chromatography on a Superdex S200 Increase column (Cytiva).