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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Protein ubiquitination was tested by immunoblots and FLAG-HRP antibody (Sigma, Catalog number A8592).
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bioRxiv - Microbiology 2023Quote: ... SPA-tagged proteins were detected with monoclonal anti-Flag M2 antibody purchased from Sigma. YbgF protein was used as an internal control and revealed with polyclonal anti-YbgF antibodies ...
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bioRxiv - Microbiology 2023Quote: ... The DNA protein complex was immunoprecipitated with either anti-FLAG monoclonal antibody M2 (Sigma) or anti-σ70 antibody (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with antibodies against OL markers proteins including Olig2 (MABN50, 1:500 Millipore, CA ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were probed with 1:2000 chicken anti-LDL primary antibody (#GW20089F, Sigma-Aldrich) diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054 ...
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bioRxiv - Biochemistry 2024Quote: ... The antibody-lysate complex was incubated with protein A- conjugated sepharose beads (Sigma #P3391) and eluted in 2X laemelli dye ...
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bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
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bioRxiv - Neuroscience 2023Quote: ... PLP (Anti-Myelin Proteolipid Protein Antibody, CT, clone PLPC1 MAB388 Sigma-Aldrich, 1:200), MHCII (Dako 1:50) ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected with an anti-FLAG antibody (1:500, Sigma F1804). Samples were also stained with DAPI (300 nM ...
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bioRxiv - Cell Biology 2020Quote: ... and permeabilized with 0.1 % triton X-100 for 10 mins for intracellular staining with a mouse or rabbit monoclonal antibody against the target protein (1h) followed by a secondary antibody conjugated with FITC- or Alexa Fluor 647 (Sigma-Aldrich) (1.100 ...
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bioRxiv - Cell Biology 2020Quote: ... 1-5ug of antibody was incubated overnight with chromatin and the complex antibody-chromatin was then captured with G protein sepharose beads (Sigma Aldrich) 1h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies: mouse anti-RSV fusion protein monoclonal antibody (MAB8599, Millipore, USA) or goat anti-human IgG (Licor ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated overnight with primary antibodies at the following concentrations: chicken anti-Microtubule Associated Protein 2 (MAP2) polyclonal antibody (Sigma-Aldrich, EMD Millipore ...
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bioRxiv - Systems Biology 2020Quote: ... Bait proteins were probed using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, F3165; used at 1:2500) in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... The levels of reovirus μ1C/δ and the PSTAIR epitope of the host protein Cdk1 were determined by Western blot using an anti-reovirus primary antibody and an anti-PSTAIR primary antibody (Sigma-Aldrich), respectively.
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bioRxiv - Immunology 2020Quote: ... Equal amounts of SG core was subject to immunoprecipitation using anti-G3BP1 antibody (1ug) and isotype specific control antibody and protein A-sepharose beads (Sigma-Aldrich). Samples were incubated at 4⁰C overnight on a rotator and immune complexes recovered by centrifugation and five washes in buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Molecular Biology 2022Quote: ... the accumulated lysates were incubated with protein A/G magnetic beads (Thermo-Fisher) pre-coupled with anti-Flag antibody (M2 monoclonal antibody, Sigma-Aldrich) for 2 h at 4°C with rotation ...
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bioRxiv - Physiology 2023Quote: Proteins of interest were labeled with well-validated custom and commercial antibodies: Connexin43 (Cx43; mouse monoclonal antibody, Cat. #: MAB3067, EMD Millipore Corp. ...
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bioRxiv - Developmental Biology 2023Quote: ... bead-bound samples were successively incubated with appropriate amount of primary antibodies against protein-of-interest (CTCF antibody: Millipore, 07-729) in 50µL of CUT&RUN antibody buffer for overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... To the remaining cell lysate was added anti-H3K4me3 antibody-coated Dynabeads M-280 Sheep anti-Rabbit IgG (5 μg antibody per sample, Millipore, 04-745), followed by rotation at 4°C overnight for immunoprecipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μg of purified His fusion protein (PuBZR1-His) was bound to Ni-NTA His binding resin (Novagen). GST fusion proteins containing PuACO1 (PuACO1-GST ...
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bioRxiv - Developmental Biology 2021Quote: ... 20 ul protein A/G beads pre-saturated with 5 mg/ml BSA in PBS (Sigma, A-3311) were washed in RIPA buffer and subsequently added to the chromatin/antibody mix at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were eluted with 5 packed bead volumes of 150 ng/μL 3xFLAG peptide (Sigma-Aldrich, F4799) in TBS for 30 min at 4 °C with rotation.
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bioRxiv - Molecular Biology 2019Quote: ... and 0.1% NP-40 substitute (Fluka)) and added to 5-7 µL of protein A sepharose beads (Sigma) in a 50% slurry from the vendor that had been washed in IPP150 and conjugated to ∼5-10 µg of affinity-purified or protein A-purified anti-Prp43p ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA-cross-linked proteins were immunoprecipitated (1% kept as input) using 5 μg of NF-kB/p65 (Millipore) or control mouse IgG antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were then trypsinised by incubating with 5 µg/ml trypsin in 25 mM triethylammonium bicarbonate (Sigma-Aldrich) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then eluted with lysis buffer 5 supplemented with 20 mM glutathione (Sigma Aldrich, product # G4521) and the elution fractions were pooled together and dialyzed into dialysis buffer (25 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of the denatured protein sample was manually spotted onto an activated PVDF Immobilon-P membrane (Millipore) mounted on top of a paper stack (bottom up ...
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bioRxiv - Genetics 2019Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl)phosphine hydrochloride (Sigma-Aldrich, St. Louis, MO, product C4706) and alkylated with 55 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was extracted from 5⨯106 cells with Pierce RIPA buffer (Thermo, 89901) containing proteasome inhibitor (Sigma, P8340) and quantified using the Pierce BCA Protein Assay Kit (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... 400 μg protein from the cytosolic lysate was incubated with 5 μg anti-FLAG mouse IgG1 (M2, Sigma) or mouse IgG1 control overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... Target proteins were eluted with 5 column volumes of wash buffer plus 200 μg/mL FLAG peptide (Sigma). The eluent was then applied to Strep-Tactin Sepharose (IBA ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% BSA) and pre-cleared with 30 μL of Protein A agarose beads (Millipore Cat No. 16-125) for 1 h at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Purified protein was dialysed against water then concentrated using an Amicon Ultra-Ultracel 5 kDa centrifugal filter (Millipore). Antibodies were raised in two guinea pigs by Eurogentec.
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bioRxiv - Cell Biology 2023Quote: ... biotinylated proteins were selectively eluted by incubating the beads in 100 µL of 5 mM dithiothreitol (DTT, Sigma), 5% SDS ...
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bioRxiv - Biochemistry 2023Quote: ... The GCL reaction was stopped by precipitation of proteins with 200 mM 5-sulfosalicylic acid (Sigma Aldrich, 247006) and then put onto ice for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 5 mL per gram of cells of a hypotonic solution (5 mM MgCl2, 0.25 mg/mL lysozyme from chicken egg white >40,000 units/mg protein (Sigma) and 1 mM phenylmethylsulfonyl fluoride) ...
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bioRxiv - Microbiology 2022Quote: ... a mouse anti-SARS-CoV-2 nucleocapsid (N) protein primary antibody and an anti-mouse IgG HRP secondary antibody in conjunction with SIGMAFAST™ OPD developing solution (Millipore Sigma) permitted SARS-CoV-2 infection quantification ...
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bioRxiv - Immunology 2023Quote: ... were detected using rabbit primary antibodies specific to the MOPV Z protein (Agrobio) and secondary alkaline phosphatase-conjugated antibodies (Sigma-Aldrich Merck). Plates were revealed using an NBT/BCiP solution (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... to avoid antibody unspecific interactions coverslips were incubated with PBS 5% Donkey Serum (Sigma #D9663), 1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... resuspended in 500 μl IP buffer mixed with 5 μg anti-m6A antibody (Millipore, ABE572) and incubated at 4 °C with gentle rotation for at least 6 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with SOX17 antibody diluted with Permeabilization Buffer and 5% donkey serum (Sigma-Aldrich) for 1 hour at +4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... All the primary and secondary antibodies were diluted in 5% normal goat serum (NGS) (Sigma) in PBT (0.03% Triton X-100/PBS) ...
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bioRxiv - Microbiology 2020Quote: ... Both primary and secondary antibodies were incubated in the presence of 5% milk (Sigma-Aldrich). HRP activity was detected with a chemiluminescence detection system (Li-COR Imaging System ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with 5% BSA in PBS+/+ and labeled with primary antibodies in PBS+/+ (D8662, Sigma) and 5% BSA overnight at 4°C and secondary antibodies for 1h RT ...
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bioRxiv - Bioengineering 2019Quote: ... Primary antibody was added which consisted of 5% (w/v) BSA (Sigma-Aldrich, A9647-10G) and either 1:1000 IDO primary antibody (BioLegend ...
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bioRxiv - Neuroscience 2019Quote: ... All primary antibodies were diluted in TBS-T or PBS-T containing 5% BSA (Sigma). Anti-rabbit or anti-mouse IgG conjugated to HRP (1:80,000 ...