Labshake search
Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Antibody-bound chromatin complex was precipitated with salmon sperm DNA/Protein A agarose (EMD Millipore, Burlington, MA), then washed sequentially with PIRA (150 mM NaCl ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were blotted as previously described and detected using Anti-polyHistidine-Peroxidase monoclonal antibody (Sigma-Aldrich # A7058) and signals were revealed using the Metal Enhanced DAB Substrate Kit ...
-
bioRxiv - Neuroscience 2020Quote: ... Proteins were separated by SDS-PAGE and probed with antibodies against zDHHC5 (1:1,000; Sigma Prestige, HPA014670), HA (1:1,000 ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
-
bioRxiv - Plant Biology 2022Quote: ... proteins and DNA fragments were then immuno-precipitated using antibodies against anti-FLAG (SIGMA, catalog no. M2), H3Ac (Millipore ...
-
bioRxiv - Biochemistry 2022Quote: ... and HA-tagged full length CASK proteins were incubated with 1μg anti-Flag M2 antibody (Sigma, F3165) at 4°C over night ...
-
bioRxiv - Plant Biology 2021Quote: ... HA-tagged proteins were detected with an Anti-HA-Peroxidase antibody (Sigma-Aldrich, St. Louis, USA, 12013819001). For the RACB ubiquitination screening ...
-
bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
-
bioRxiv - Bioengineering 2019Quote: RanBP9 was immunoprecipitated from total protein extracts using monoclonal αHA-Agarose antibody (Sigma cat. nr. A2095-1ML). Briefly ...
-
bioRxiv - Plant Biology 2021Quote: ... the proteins underwent binding with secondary antibodies diluted 5000-fold in PBS (anti-rabbit IgG; Sigma-Aldrich). Protein bands were visualized by staining with Clarity Western ECL Substrate (Bio-Rad ...
-
bioRxiv - Physiology 2021Quote: ... Protein was detected using a secondary HRP-linked antibody and Luminata Classico Western HRP Substrate (Millipore WBLUC0020). To reprobe membranes ...
-
bioRxiv - Plant Biology 2021Quote: ... Flag-tagged proteins were detected using the anti-Flag HRP-coupled antibody (mouse monoclonal, A8592; Sigma-Aldrich) diluted 1:5000 (v/v) ...
-
bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
-
bioRxiv - Evolutionary Biology 2020Quote: ... we employed antibodies against RNA-Binding Protein RBFOX3 (aka NeuN; mouse anti-NeuN, Alexa488-conjugated, MAB377X, Millipore) that is expressed in all neuronal nuclei ...
-
bioRxiv - Microbiology 2021Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
-
bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunodetection of ProteinA (ProtA)-tagged proteins was performed using Peroxidase Anti-Peroxidase Soluble Complex antibody (Sigma-Aldrich) in a 1:3,000 dilution ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were detected by western blotting with antibodies mab2502 (clone 26) and mab1919 (clone 11C1.3) from Sigma.
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
-
bioRxiv - Biochemistry 2023Quote: ... total protein was extracted and examined via western blots using HRP-conjugated anti-mouse secondary antibody (Sigma). Protein loading was visualized with Amido Black Staining Solution (Sigma).
-
bioRxiv - Neuroscience 2023Quote: ... Monoclonal antibodies were purified from the cell culture supernatants by affinity chromatography on Protein G Sepharose (Sigma), using 0.1M glycine ...
-
bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for detection of proteins by western blot: anti-M2-FLAG (Sigma, F3165), anti-β-tubulin (Sigma ...
-
bioRxiv - Developmental Biology 2024Quote: ... Protein G magnetic beads (TAMAGAWA SEIKI, TAS8848N1173) were pre-incubated with anti-FLAG antibody (SIGMA [M2], F1804) for more than 1 h at 4°C with gentle rotation before adding lysate ...
-
bioRxiv - Developmental Biology 2024Quote: ... TBX33xFLAG proteins were detected by chemo-luminescence using monoclonal M2 anti-FLAG antibodies (1:500, Sigma, F1804) in combination with donkey anti-mouse IgG-HRP antibodies (1:5000 ...
-
bioRxiv - Microbiology 2024Quote: ... was used as second antibody and proteins on the membrane were visualized using BCIP/NBT substrate (Sigma). When deer plasma specimens were used as primary antibody ...
-
bioRxiv - Cell Biology 2020Quote: ... and 20 µg total protein was denatured for 5 min at 70°C in 1X Laemmli Buffer (Sigma Cat # S3401-10VL), resolved on SDS-acrylamide gels and electroblotted to nitrocellulose ...
-
bioRxiv - Neuroscience 2019Quote: ... After protein transfer blots were blocked in 5% BSA/TBS for 1 h and stained for Sox2 (1:100, Millipore AB5603), Sox9 (1:250 ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Lysate was heat-treated at 70 °C for 5 minutes to denature proteins after which one 20 μl aliquot was mixed with 20 μl Triglyceride Reagent (Sigma T2449) and one 20 μl aliquot was mixed with 20 μl of PBS and incubated at 37 °C for 30 minutes ...
-
bioRxiv - Genomics 2020Quote: Cells growing in log phase were harvested for protein extraction using the protein extraction buffer (50 mM Tris–HCl, 150 mM NaCl, 1% Triton-X, 10% glycerol, 5 mM EDTA; all Sigma-Aldrich) and 0.5 μl protease inhibitor mix (Sigma-Aldrich) ...
-
bioRxiv - Systems Biology 2020Quote: ... Acetone was discarded and protein pellets were air dried for 5-10 min and then resuspended in 100 μL of 50 mM TEABC (Sigma-Aldrich) Overnight enzymatic digestion was carried out using sequencing grade trypsin (Promega ...
-
bioRxiv - Immunology 2021Quote: ... KLH protein was dissolved in PBS to 5 mg/mL of and then mixed 1:1 with CFA (both Sigma-Aldrich) upon which 40 μL of the immunization mixture was injected in footpads and flanks of draining popliteal and inguinal LNS ...
-
bioRxiv - Microbiology 2021Quote: ... The beads were washed 5 times in RIPA buffer and the immunoprecipitated proteins eluted using 150 μg/mL 3x Flag peptide (Sigma-Aldrich) in elution buffer (50 mM Tris/HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... The resin was washed with 40 bed volumes of buffer A and the fusion protein was eluted with buffer A containing 5 mM d-Desthiobiotin (Sigma-Algrich). Peak atEH domain fractions were dialyzed overnight at 4°C against buffer A in the presence of TwinStrep-tagged TEV protease (32 ...
-
bioRxiv - Cancer Biology 2022Quote: ... was added to each sample and protein disulphide bonds were reduced with 5 µL of 200 mM Tris(2carboxyethyl) phosphine (TCEP, Sigma-Aldrich) solution and incubated at room temperature for 1.5 hours ...
-
bioRxiv - Systems Biology 2019Quote: ... The extracted proteins were analyzed by 5-20% gradient SDS-PAGE and transferred onto Immobilon transfer membranes (Millipore, Billerica, MA, USA) for immunoblotting analyses ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were washed twice with cold phosphate-buffer saline (PBS) and total proteins were precipitated in 5% trichloroacetic acid (TCA) with 1 mM Methionine (Met) (Sigma-Aldrich) for 15 minutes on ice ...
-
bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The FLAG-AGO1 proteins were detected with anti-FLAG-HRP (1:4000 [Sigma] in 5 % milk in PBS-T [0.05% tween20]) or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T ...
-
bioRxiv - Microbiology 2023Quote: ... Washed beads were adjusted to pH 7.5 with 200 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) and bound proteins were reduced using 5 mM dithiothreitol (Sigma-Aldrich) at 37°C for 1 h ...
-
bioRxiv - Developmental Biology 2023Quote: ... All proteins were separated in 5-22% gradient SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (PVDF, Millipore, Billerica, MA) using a semi-dry transfer apparatus (Hoeffer) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Approximately 1.5 mg of proteins from each sample was reduced with TCEP (5 mM, room temperature for 10 min, Cat# 580561, Millipore Sigma) then alkylated with chloroacetamide (20 mM ...
-
bioRxiv - Bioengineering 2023Quote: ... The hybridized RNAs were then mixed with 25 µL of 30 µM (5 mg/mL) recombinant SpCas9 protein (Sigma-Aldrich #Cas9PROT) for 30 minutes at 4 C ...
-
bioRxiv - Immunology 2023Quote: ... The capture matrix was washed three times with PBS and incubated over night with 1:5 diluted plasma samples in a low protein binding MultiScreen® filter plate (Millipore). Depleted plasma was recovered by centrifugation of the filter plate ...
-
bioRxiv - Biochemistry 2023Quote: Studies of SAMHD1-controled dynamic equilibria of dNTP concentrations (Fig. 5) were performed using a 10 mL stirred-cell pressurized protein concentrator (Amicon; EMD Millipore) assembled with a 10 kDa MWCO nitrocellulose membrane (Millipore) ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1 nL of 12.5 ng/µL of sgRNA and 1 nL of Cas9 protein (300 ng/µL)64 along with phenol red (Sigma Aldrich) were co-injected in one-cell stage embryos ...
-
bioRxiv - Biochemistry 2023Quote: ... No. H7379) and human hemoglobin A0 (HbA0) (with 5% (w/w) methemoglobin) (Cat. No. H0267) (all protein samples from Sigma, USA). Tetrahydrofuran (THF ...
-
Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: ... isolated mitochondria equivalent to 1 mg protein were mixed with 250 μl methanol before adding 5 mL 0.1M Hydrochloric acid (Sigma, Cat# 1090601000) and 100 µL SPLASH (Avanti Polar Lipids ...
-
bioRxiv - Cancer Biology 2021Quote: 5-Fluoro-5’-Deoxyuridine (Sigma Aldrich, F8791) and Oxaliplatin (Sigma Aldrich ...
-
bioRxiv - Microbiology 2021Quote: ... 5 ml of HNO3 5% (Sigma, 84378) were added to each sample and they were placed 1h at 95°C ...