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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The following antibodies were used for specific protein detection: anti-PAP (1: 1:5000, rabbit, Sigma), anti-myc (mouse ...
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bioRxiv - Cancer Biology 2020Quote: ... The pre-cleared protein solution was incubated with 3 μg anti-FLAG antibody (Sigma Aldrich, #F1804) or IgG (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and mouse monoclonal anti-glial fibrillary acidic protein antibody (G3893, Sigma-Aldrich, St Louis, MO, USA). Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total protein was then incubated with anti-Flag antibody (M2 monoclonal clone, Sigma) for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Molecular Biology 2022Quote: ... The precleared lysate was then transferred to Protein G Dynabeads coupled with anti-FLAG antibody (Sigma) and the mixture was incubated for 2 hours at 5°C on a roller ...
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bioRxiv - Immunology 2022Quote: ... coupled to protein A Sepharose magnetic beads (Ademtech 0423) and anti-TAX1BP1 antibody HPA024432 (Sigma-Aldrich) together with protein A Sepharose (GE Healthcare) ...
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bioRxiv - Genomics 2020Quote: ... except for the following modifications: immunoprecipitation was carried out using monoclonal antibodies against protein A (Sigma); the lysis buffer contained 10mg/ml heparin (sigma H7405) ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Physiology 2022Quote: ... protein G beads were coupled to the pre-pro-vasopressin antibody or normal rabbit serum (Sigma) at a 1:50 ratio for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... The immunoprecipitation was performed by incubating the protein lysate with 1ug of GluA1 antibody (Millipore, ab1504) or 1ug HA antibody (Santa Cruz ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: ... Puromycin incorporation into neosynthesized proteins was assessd with Western blotting and an anti-puromycin antibody (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
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bioRxiv - Biochemistry 2021Quote: ... and protein was detected by Western blot analysis using primary αRb H3K27me3 antibody (Millipore #07-449), secondary antibody (Biorad #170-6515) ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation was carried out with β-catenin monoclonal antibody and Protein G magnetic beads (Sigma-Aldrich). For PCR ...
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bioRxiv - Cell Biology 2022Quote: ... The same membrane was stripped of antibody and total protein was determined with Coomassie (Sigma, B0149) staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using chemiluminescence from horseradish peroxidase-coupled secondary antibodies Anti-rabbit HRP (Sigma; A0545), 1:15,000.
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bioRxiv - Bioengineering 2022Quote: ... Protein bound to the wells were detected with a peroxidase conjugated anti-Flag antibody (Sigma A8592) and quantified with QuantaBlu™ Fluorogenic Peroxidase Substrate Kit (Thermo Scientific™ 15169) ...
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bioRxiv - Cancer Biology 2022Quote: ... 50µL of protein G beads were coated 2ug of anti-AGO2 antibody (Sigma SAB4200085 Lot# 0000089486) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Microbiology 2023Quote: ... GST and GST-LegK2 proteins were labelled with an anti-GST antibody from rabbit (A7340; Sigma) and detected with an anti-rabbit secondary antibody from goat conjugated with the fluorochrome Alexa Fluor 488 (A11034 ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... or anti-v5 antibody and 40 µl of Protein G agarose beads slurry (Millipore, 16-266) in the presence of RNase A (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... The seven target proteins and antibodies for the sFANSeq panel included Neuronal Nuclei Antigen (NeuN, Millipore Cat# ABN91 ...
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bioRxiv - Molecular Biology 2023Quote: Antibodies to human proteins used in this study included: Actin (1:20,000 dilution (MAB1501, Millipore, RRID:AB_2223041)) ...
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bioRxiv - Molecular Biology 2023Quote: ... The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T). The intensity of the signal from retained anti-AGO1-N-coil antibody was quantified using Image J and fitted using Nonlinear Least Square (NLS ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Genomics 2021Quote: ... the aliquot of sheared chromatin fragments were incubated with antibody conjugated Protein A Dynabeads using total histone 3- (unmodified H3 antibody, Millipore #05-499; 1:1000) or histone 4- (unmodified H4 antibody ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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bioRxiv - Physiology 2019Quote: ... Hydrolyzed protein diet was prepared by incubating 5 mL intact protein diet with 43.7 mg trypsin (Trypsin Powder, Porcine 1:250, 85450C, Sigma Aldrich) at 37°C for 20 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... A volume of 12.5 μl of a solution containing 500 ng protein was diluted in H2O with 5× Sypro Orange (Sigma-Aldrich) and immediately mixed with an equal volume of assay condition ...
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bioRxiv - Neuroscience 2019Quote: Total protein was extracted from L4/5 DRGs or retinas using the RIPA buffer containing protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: To expose Prdx2 to continuous H2O2 generation the protein (20 μM) was incubated with 5 mM glucose plus glucose oxidase (Sigma). Glucose oxidase was diluted to produce approximately 0.5 μM H2O2/min.
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bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: The fractions containing eluted proteins were concentrated to ∼5 ml by AmiconTM ultrafiltration devices (10 kDa or 30 kDa molecular weight cut-off) (Millipore) and centrifuged in a bench centrifuge for 10 min at maximum speed to remove any precipitated material before being subjected to size-exclusion chromatography (SEC) ...
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bioRxiv - Cancer Biology 2022Quote: ... Digested DNA was then incubated with 50 µl of protein A/G agarose beads and 5 µl anti-S9.6 (Millipore, MABE1095) overnight at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... The purified protein was concentrated up to 5 mg/mL using Centricon YM-10 and Microcon YM-10 concentrators (Amicon, Millipore). Crystals used in this study were obtained under oxygen exclusion in hanging drops from 0.5 M Hepes pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: The induction of the unfolded protein response was carried out by the addition of 5 µg/ml tunicamycin (Sigma-Aldrich) in medium and incubation for 6 h prior cell lysis or the supplementation of pre-warmed medium with 10 mM DTT (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... the as-isolated proteins (25 μM) were incubated under aerobic conditions with the ferric chelator desferrioxamine (5–1000 mM, Sigma)(11) ...
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bioRxiv - Genetics 2021Quote: ... the resin was washed 5 times with ice cold DOC buffer and the bound protein eluted by 15 minute 70°C incubation in 5% w/v sodium dodecyl sulphate (SDS, Sigma). The eluted protein was reduced with 10 mM TCEP and alkylated using 20 mM Iodoacetamide ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) (Transgen, CD601-02) and protein expression was induced by an auto-induction system (71757-5, EMD Millipore). Upon overnight induction at 25°C ...
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bioRxiv - Cell Biology 2019Quote: ... Protein was transferred to a PVDF membrane (0.2 micron, Immobilon) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 mins ...
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bioRxiv - Genomics 2021Quote: ... 5 mM sodium metabisulfite and 1 mM MgCl2) containing 100 μg of purified protein and 100 μM GGPP (Sigma-Aldrich). The reaction mixture was overlaid with 500 μL of pentane (Macklin ...