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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were centrifuged at 10,000 g for 5 minutes and total protein in the supernatant was measured using the bicinchoninic acid (BCA) assay (Sigma). Primary antibodies were detected using a secondary antibody conjugated to horseradish peroxidase ...
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bioRxiv - Immunology 2020Quote: ... The purified protein was concentrated to about 5-10 mg/ml using Amicon concentrators with 30K MWCO (Millipore, Etobicoke, ON), and exchanged into the desired buffer ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: The nitrocellulose membrane was imaged for total protein and then blocked for one hour in blocking buffer (5% w/v bovine serum albumin [Sigma, SKU A7906] in TBS-T buffer [50mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Physiology 2023Quote: ... Supernatants from cells overexpressing Fc-tagged FGL1 proteins were collected after 5 days and supplemented with protease inhibitor cocktail (Sigma). Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The insoluble matter was removed by centrifugation 10000×g for 5 min at 4 °C and protein was estimated using BCA kit (Sigma) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
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bioRxiv - Microbiology 2023Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell lines stably expressing the WT/mutant proteins were selected using media supplemented with 5 μg/ml puromycin (Sigma-Aldrich). The reconstitution of mutant GSECs was assessed by SDS-PAGE/western blotting ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... Each target protein was buffered and concentrated in 5 mM MOPS (pH 7.0) by ultrafiltration with AmiconUltra (Millipore, MA, USA) or VivaSpin (Sartorius ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was transferred to a PVDF membrane 0.2 μm (Amersham) and blocked in 5% Bovine Serum Albumin (BSA) (Sigma-Aldrich) for 20 min ...
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bioRxiv - Genetics 2022Quote: ... 5’-fluoroorotic acid (5’FOA; Sigma) was used at a concentration of 1 g/L in PMG containing 45 mg/L uracil ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was then blocked with 5% BSA and incubated with a primary antibody (Monoclonal ANTI-FLAG® M2 antibody, 1:1000; from Sigma-Aldrich, St. Louis, MO) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... and then incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma) at RT for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Nonspecific antibody binding was blocked by incubation with TBS containing 5% (w/v) dry milk (Sigma Aldrich) for one hour at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were diluted in blocking solution containing 5% Normal Donkey Serum (Sigma-Millipore, cat. no. D9663) and 0.1% Triton X-100 in PBS and incubated overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were diluted in blocking solution containing 5% Normal Donkey Serum (Sigma-Millipore, cat. no. D9663) and 0.1% Triton X-100 in PBS and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody incubation was performed overnight at 4°C diluted in 5% BSA (Sigma-Aldrich, MO, USA) in TBS-T ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All antibodies were diluted in TBST with 5% bovine serum albumin (Sigma Aldrich, St. Louis, MO, USA). Blots were washed in TBST and probed with IRDye 680RD donkey anti-mouse (AB_10953628 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were labeled in 5% BSA/TBST with either anti-HA peroxidase conjugated antibodies (Sigma, 1:200) or rabbit TgALD1 antibody (1:200 ...
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bioRxiv - Cell Biology 2020Quote: For immunofluorescence we used the following primary antibodies: rabbit anti-LAT (5 μg/ml, 06– 807; Millipore), human anti-GFP (produced by the Institut Curie platform) ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies for immunofluorescent staining were as follows: vinculin (clone VIN-11-5, Sigma or clone 42H89L44, Invitrogen); phosphotyrosine (clone 4G10 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked in 5% milk and probed with the following antibodies: anti-Flag M2 (SIGMA, #F1804) and anti-GAPDH (clone 6C5 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated with a rabbit polyclonal antibody against 5-HT (Sigma; S5545; final dilution = 1:3,000). After few washes ...
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bioRxiv - Physiology 2021Quote: ... standard one spot MSD plates were incubated with 5 μg/mL of capture antibody (Cat# HPA018884; Sigma) overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Label nuclei by resuspending pellet in 5 mL blocking buffer with NeuN-488 antibody (Millipore, cat: MAB377X) and OLIG2 antibody (Abcam ...
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bioRxiv - Immunology 2019Quote: ... 5 μg of the following antibodies were used: H4ac (Histone 4 pan-acetyl, Merck Millipore #06-866) anti-H3K27ac (Abcam ab4729) ...
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bioRxiv - Cell Biology 2019Quote: ... Coverslips were washed 3 times for 5 min then incubated with secondary antibodies and Hoechst 33258 (Sigma) for 1 hour at room temperature and again washed ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 5 μL of H3K4me3 (histone H3 lysine 4 trimethylation) antibody (Millipore, 17-614) and incubation overnight at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were incubated overnight with Rabbit anti-p-SMAD1/5/8 primary antibody (AB3848, Millipore-Sigma) in a solution of 2.5% BSA/0.5% goat serum at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... 5–10 × 10−15 mol DNA substrate was mixed with ~200 ng anti-digoxigenin antibody (Sigma-Aldrich) in PBS and incubated in a sample cell for one hour at room temperature or overnight at 4° C ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were incubated overnight with Rabbit anti-p-SMAD1/5/8 primary antibody (AB3848, Millipore-Sigma) in a solution of 2.5% BSA/0.5% goat serum at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... the following antibodies diluted in 5% milk in TBST were used: KEAP1 (Millipore Sigma, RRID:AB_2921362, 1:2000), NRF2 (Cell Signaling Technologies ...
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Physiology 2021Quote: ... 500 μg of lysates/mouse were then immunoprecipitated with anti-PKG antibody(19) and protein conjugated-Sepharose beads and blotted with anti-NT antibody (Millipore, Cat#05-233, 1:2000). The same membrane was also blotted with anti-PKG antibody as a loading control ...
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bioRxiv - Neuroscience 2023Quote: The following primary antibodies were used for immunofluorescence (IF) in this study: a monoclonal antibody (mAb) against tau protein (clone Tau-1; Millipore, Cat Number: MAB3420, RRID: AB_94855) diluted 1:1000 ...
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bioRxiv - Plant Biology 2020Quote: Proteins were resolved on SDS-PAGE followed by immunoblotting using α-HA high affinity antibody 3F10 (Sigma) and α-c-Myc Antibody (9E10) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were purified using anti-HA affinity matrix (Sigma; rat anti-HA clone 3F10 antibodies) and FLAG-tagged proteins were purified using anti-FLAG M2 affinity gel (Sigma ...
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bioRxiv - Genetics 2020Quote: ... FLAG-tagged LIR-2 protein was detected with monoclonal anti-FLAG M2-Peroxidase (HRP) antibody (SIGMA-Aldrich) and EGFP:NHR-25 was visualized with Clarity Western ECL substrate (Bio-Rad) ...
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bioRxiv - Biophysics 2020Quote: ... Separated proteins were transferred to a nitrocellulose membrane and immunoblotted with an antibody recognizing Orai1 (Sigma Aldrich). Each experiment was performed at least 3 independent times and analysed with the program ImageJ (NIH ...
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bioRxiv - Cell Biology 2019Quote: ... Effects on protein synthesis rates were assessed by SDS-PAGE and immunoblotting using puromycin antibody (Millipore, #MABE343).
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bioRxiv - Plant Biology 2019Quote: ... following which the proteins were transferred from gel to membrane and probed using Anti-FLAG antibody (Sigma).
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bioRxiv - Cell Biology 2019Quote: ... blotted onto nitrocellulose and the proteins were detected with an anti-His antibody (Sigma-Aldrich, Steinheim, Germany). To test whether 6His-PBBem1 and Cdc11 can bind simultaneously to Cdc24428-854 ...
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bioRxiv - Microbiology 2019Quote: ... This magnetic resin was generated by incubating Dynabead Protein G (ThermoScientific) with anti-FLAG antibodies (Sigma Aldrich) at room temperature followed by washing ...
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bioRxiv - Biochemistry 2020Quote: ... FLAG-tagged proteins were detected with horseradish peroxidase (HRP)-conjugated anti-FLAG antibody (Sigma Aldrich, A8592-1MG). All western blots were developed using the ECL Plus Western Blotting Detection System (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... 1 μg fibrillarin 2 or NOP56 antibody was coupled to 30 μL protein-A agarose beads (Sigma) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Total protein synthesis was measured by immunoblot using the anti-puromycin antibody at 1:500 (EMD Millipore). Protein synthesis was quantified by densitometry using ImageJ and normalized by immunoblot using antibody specific to steady-state levels of eIF2α protein (13).