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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... The gRAMP gene and the synthetic CRISPR gene were cloned into the pRSFDuet-1 vector (Novagen, kanamycin resistance), in which an N-terminal hexahistidine tag was fused with gRAMP ...
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bioRxiv - Molecular Biology 2021Quote: ... and TniQ were expressed in Escherichia coli BL21(DE3) while TnsC was expressed in Rosetta™(DE3)pLysS (Novagen: #70956) containing a pLysS-tRNA plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... 500,000U Recombinant Mouse LIF Protein (Millipore Cat#ESG1107)) with feeders ...
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bioRxiv - Developmental Biology 2024Quote: ... and ubiquitinylated proteins (clone FK2, mouse, Sigma-Aldrich) at 1:200 dilution were added ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... and expressed in Escherichia coli Rosetta cells (Novagen). The recombinant proteins were affinity purified through GST tag binding to amylose resin (BioLabs ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Bioengineering 2021Quote: ... Serum pramlintide concentrations were quantified using a human amylin ELISA kit (Millipore Sigma). Serum lispro concentrations were quantified using Northern Lights Mercodia Lispro NL-ELISA ...
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bioRxiv - Cancer Biology 2020Quote: ... In the RAS activation assay kit for ELISA (Cat.#17-497; EMD Millipore), 12 µg total protein was used at 100 ng/µL and the RAS-GTP pull-down was measured using a Synergy H1 plate reader (BioTek ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured with Human Insulin ELISA kit (Millipore Sigma). See Table S1 for donor information.
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bioRxiv - Biochemistry 2019Quote: ... Plasma leptin levels were analysed with an ELISA kit (Millipore, Billerica, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Level of serum insulin was determined using insulin ELISA kit (Millipore-EZMRI-13K).
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bioRxiv - Cell Biology 2023Quote: ... These evaluations were performed by the commercial Human ELISA kits (Sigma-Aldrich, USA) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasma leptin and insulin levels were measured by murine ELISA kits (Merck Millipore, Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... which was analyzed by enzyme linked immunosorbent assay (ELISA) kit (Sigma-Aldrich, MO). The levels of Citrine-H3 (Cit-H3 ...
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bioRxiv - Biophysics 2021Quote: J774A.1 macrophages (ATTC, number TIB-67) were grown at 37°C in DMEM (Sigma Aldrich, USA) containing 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 monocytes were differentiated into macrophages with 50ng/ml PMA (Phorbol 12-myristate 13-acetate) (Sigma) for 16 hours followed by 24 hours of incubation in complete medium (Mehto et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The macrophage activation was done with the treatment of 1 ng/mL lipopolysaccharide (L2630, Sigma Aldrich, USA) to RAW264.7 or 2 ng/mL transforming growth factor β-1(240-B/CF ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 monocytes were differentiated into macrophages with 100 ng/mL phorbol 12-myristate 13-acetate (Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2022Quote: METTL3-depleted and control THP-1-derived macrophages were treated with 10 μg/ml actinomycin D (Sigma) for 2 ...
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bioRxiv - Cancer Biology 2022Quote: ... THP-1 cells were differentiated into macrophages using 100ng/ml phorbol 12-myristate 13-acetate (Sigma P8139) for 3 days ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Frozen stocks of macrophage THP-1 cells (ATCC TIB-202) were thawed in RPMI-1640 medium (Sigma) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 cells were stably differentiated into macrophages by phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) at 5 ng/ml over 48h ...
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bioRxiv - Immunology 2020Quote: 5 × 105 macrophages were fixed in 2.5% glutaraldehyde (Sigma) in 100 mM sodium cocodylate (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Macrophages were then harvested by incubation with Accutase (Sigma) at 37 °C to gently detach cells followed by centrifugation at 300 x g for 10 minutes ...
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bioRxiv - Immunology 2022Quote: Macrophages were plated on poly-L-lysine (Sigma-Aldrich)-coated coverslips ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were plated on poly-L-lysine (Sigma-Aldrich)-coated coverslips ...
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bioRxiv - Molecular Biology 2023Quote: Macrophages were lifted from plates using Accutase (Sigma-Aldrich) and immediately incubated with 1:1000 Zombie-NIR (BioLegend ...
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bioRxiv - Bioengineering 2024Quote: ... macrophages were harvested after incubation with Accutase (Sigma-Aldrich) for 15 minutes along with gentle cell scraping.
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bioRxiv - Molecular Biology 2023Quote: ... band intensity was first normalized to the housekeeping gene tubulin (mouse anti-tubulin, Sigma T9026), and then in order to compare between blots ...
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bioRxiv - Developmental Biology 2022Quote: A peptide corresponding to residues 1 – 159 of HyYap was expressed as GST-fusions using the GEX4t-1 vector (Millipore) in E ...
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bioRxiv - Immunology 2021Quote: Transiently expressed antibodies were purified from 30 ml of HEK 293 Expi cultures using Protein G GraviTrap columns (Sigma, GE28-9852-55) and the Ab Buffer Kit (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Mm-RAB8A-Q67L and GST-Rn-RAB27A-Q78L proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore, Novagen, Darmstadt, Germany). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Biochemistry 2023Quote: ... Fusion proteins were expressed in Escherichia coli and subsequently purified using glutathione high-capacity magnetic agarose beads (Merck Millipore GmbH, Darmstadt, Germany) following the manufacturer’s guidelines.
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bioRxiv - Cell Biology 2022Quote: 96-well ELISA plates were coated overnight at 4°C with anti-mouse total β1-integrin (Millipore) or anti-mouse β1-integrin ...
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bioRxiv - Biochemistry 2023Quote: ... diluted 1:10,000 in ELISA buffer (PBS, 5% Tween20, 1% goat serum (Sigma-Aldrich, G9023)) was added to all wells ...
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bioRxiv - Microbiology 2023Quote: ... both genes were inserted into the pETDueT-1 plasmid (Novagen) in two steps ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with either HRP-linked goat anti-mouse IgG antibody (dilution 1:10,000; Sigma-Aldrich) or HRP-linked donkey anti-rabbit IgG antibody (dilution 1:10,000 ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then detected with monoclonal mouse anti-FLAG M2 (1:1000; Sigma-Aldrich, St. Louis, MO) and polyclonal rabbit anti-6xHis (1:2,000 ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were detected using the primary antibody (Mouse α-His at 1:2500 dilution, Sigma Aldrich) followed by the HRP conjugated secondary antibody (Sheep α-Mouse at 1:7000 dilution ...
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bioRxiv - Developmental Biology 2019Quote: ... The target protein levels were normalized to the endogenous control (GAPDH, mouse, Millipore Sigma, MAB374, 1/500). The membranes were scanned on the LI-COR Odyssey CLx imaging system.
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bioRxiv - Genetics 2020Quote: ... Equal protein loading was confirmed by probing with mouse-anti-β-actin antibody (1:10,000; Sigma, A5441).
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Biochemistry 2024Quote: ... Gfp-tagged protein of interest was detected using a primary mouse anti-Gfp (1:3,000, Millipore/Sigma). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Genomics 2019Quote: ... Expressed TUG1 protein was stained for 1 hour at room temperature using a monoclonal anti-FLAG mouse antibody (1:500, F1804, Sigma Aldrich) and co-stained with organelle markers for mitochondria (1:1000 ...