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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Total GLP1 protein in brain tissue or in plasma was determined using GLP-1 Total ELISA 96-Well Plate Assay (Sigma-Aldrich, EZGLP1T-36K). The assay was conducted according to manufacturer’s protocol ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Mycoplasma was monitored in the U937 macrophages using the Venor GeM Mycoplasma Detection Kit (Sigma-Aldrich #MP0025). Two days prior to challenge with L ...
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bioRxiv - Biochemistry 2021Quote: ... laforin was expressed in BL21 (DE3) (Novagen) E ...
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bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
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bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
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bioRxiv - Biophysics 2021Quote: ... The protein was expressed and purified the same way as SARS-CoV Mpro-2 but Factor Xa protease (Sigma, Canada) was used (4 °C ...
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Biochemistry 2020Quote: Rabbit polyclonal anti-TcHTE antibodies were obtained using the p1TcHTE peptide (amino acid 120 to 171) expressed as a TRX.HIS-fusion protein in the vector pET-32a vector (Novagen®), following same strategy we described previously (23) ...
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bioRxiv - Cell Biology 2019Quote: ... from bacteria were performed as previously described (Llauró et al., 2018) except that proteins were expressed in Rosetta 2(DE3)pLys cells (Novagen).
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bioRxiv - Molecular Biology 2022Quote: Recombinant proteins used for biochemical assays were expressed from pMal-RT plasmids (see above and Table S3. For each protein preparation, E. coli Rossetta2 CapR cells (Novagen) containing freshly transformed expression constructs were plated on LB agar containing ampicillin (100 µg/mL ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins were expressed by induction at exponential growth phase (OD600nm≈0.5) with 0.5 mM isopropyl-β-D-thiogalactopyranoside (Sigma, I6758) for 2.5 h at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Proteins guided towards high cysteine content were transformed into and expressed in Rosetta-gami B(DE3) Competent Cells (Novagen 71137). The 1 mL and 50 mL scale protein purification protocols were otherwise followed.
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bioRxiv - Cell Biology 2023Quote: Rho family GTPase proteins were expressed using a modified pGEX vector transformed into Rosetta-Gami B BL21(DE3) pLysS strains (Novagen). Protein was purified from these cells by freeze-thaw lysis in Hepes buffer (20mM Hepes ...
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bioRxiv - Molecular Biology 2024Quote: Full length IMP2 protein was expressed as a pET-45 6-Histidine tagged 3C protease cleavable protein in Rosetta (DE3) pLysS Escherichia coli (Novagen) cells at 18 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... 15N] triple-labeled proteins for NMR assignments at pH 5.0 were expressed in M9 minimal medium prepared using 100% D2O (Sigma-Aldrich) as solvent (M9 D2O medium) ...
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bioRxiv - Molecular Biology 2021Quote: ... THP-1-derived macrophages were then stimulated for 24 hours with 1 μg/mL LPS (Sigma, St. Louis, MO), followed by a 24-hour incubation with 1×1010 particles/mL Exo-101 ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Systems Biology 2023Quote: ... THP-1 cells were stimulated with either 100ng/mL (for M1 macrophages) or 50ng/mL (for M2 macrophages) of phorbol 12-myristate 13-acetate (PMA, Sigma) for 48h ...
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bioRxiv - Neuroscience 2021Quote: ... Puromycin-labeled proteins were identified using the mouse monoclonal antibody (1:10,000, Millipore, catalog MABE343). Protein synthesis levels were determined by analyzing total lane density from 10 kDa to 250 kDa ...
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bioRxiv - Microbiology 2019Quote: ... PVDF membrane-bound proteins were first probed with mouse anti-HA (1:3000; Sigma, H3663) and rabbit anti-GAPDH (1:5000 ...
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bioRxiv - Physiology 2021Quote: ... Japan) and anti-glial fibrillary acidic protein (GFAP) (1:2,000; mouse monoclonal, G3893, Sigma-Aldrich) were used to assess activated microglia and reactive astrocytes in ischemic regions ...
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bioRxiv - Neuroscience 2019Quote: ... monoclonal anti-mouse microtubule-associated protein 2 (MAP2; Millipore Sigma, Burlington, MA; 1:1000 dilution), and monoclonal anti-mouse primary conjugated-cy3 glial fibrillary acidic protein (GFAP ...
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bioRxiv - Neuroscience 2022Quote: ... commercially available ELISA kits were used to measure lactose content (Sigma-Aldrich Cat ...
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bioRxiv - Immunology 2021Quote: ... which was analyzed by enzyme linked immunosorbent assay (ELISA) kit (Sigma, MO).
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bioRxiv - Developmental Biology 2022Quote: ELISA kits were used for measurements Growth Hormone (Merck-SIGMA EZRMGH-45K) according to manufacturers’ instructions.
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bioRxiv - Neuroscience 2022Quote: ... S100B ELISA kits were purchased from Millipore (EMD Millipore, St. Louis, MO) and samples were diluted 1:1 ...
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bioRxiv - Neuroscience 2023Quote: ... Plasma was processed using an insulin ELISA kit (Millipore Sigma; #EZRMI-13K) following manufacturer’s instructions in duplicate ...
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bioRxiv - Cell Biology 2019Quote: ... Gene Tex) antibody was incubated with protein G coated magnetic beads (PureProteome protein G magnetic beads, LSKMAGG10, EMD Millipore Corp.) for 1-3 hrs in IP (immunoprecipitation ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin B1 shRNA was expressed from pLKO.1 shRNA-LMNB1.71 puro (SHCLND-NM_005573, Sigma-Aldrich) containing the sequence ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: E-cad perturbations were expressed using the pLKO.1 non-target scramble (#SHC016, Millipore Sigma) and E-cad shRNA (TRCN0000237841 ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Biochemistry 2024Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated proteins were captured in ELISA plates pre-coated with 5 µg/mL of Avidin (Sigma) in Na2CO3/NaHCO3 buffer pH 9.6 ...
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bioRxiv - Microbiology 2020Quote: ... CCL2 and CCL5 protein levels were quantified using a sandwich ELISA procedure (Sigma, Cat# RAB0055 & RAB0077) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the M2 macrophages were treated with 1 μg/mL lipopolysaccharides (LPS) (Sigma, Saint Louis, MO, USA) or 40 ng/mL IL-4 (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... For brain targeted gene manipulation TTR esiRNA (esiRNA targeting mouse Ttr- EMU030721, Sigma Aldrich)–jetPEI complex was infused into hypothalamus ...
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bioRxiv - Cancer Biology 2022Quote: ... GM-CSF cultured macrophages were polarized to M1 macrophages with 20 ng/ml IFN-y (Prepotech) and 100 ng/ml LPS (Sigma-Aldrich), while M-CSF cultured macrophages were polarized to M2d macrophages with 10 ng/ml M-CSF and 20 ng/ml IL-6 (Prepotech) ...
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bioRxiv - Immunology 2021Quote: ... were differentiated into macrophages for six days using 50ng/mL granulocyte-macrophage colony-stimulating factor and 25ng/mL interleukin-10 (Sigma, USA), as described previously [24].
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bioRxiv - Microbiology 2021Quote: ... for ELISA (Sigma) was added to visualize the reaction ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged SMOCtail and IFT38 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Molecular Biology 2022Quote: ... Isotopically labelled proteins were expressed using Escherichia coli BL21 DE3 cells in M9 minimal media supplemented with 15NH4Cl (Sigma-Aldrich ISOTEC). Chaetomium thermophilum Naa5082-289 containing a C-terminal His-tag was expressed using E ...
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bioRxiv - Microbiology 2023Quote: ... THP-1-derived macrophages were obtained by treating THP-1 cells with 20 ng/ml phorbol myristate acetate (Sigma, P8139) for 24 hours ...
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bioRxiv - Cell Biology 2023Quote: ... The stripped membrane was re-blotted for a reference protein using 1:5000 monoclonal mouse anti-mouse alpha-tubulin (Sigma, T6199-25UL), overnight at 4°C ...