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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and the Bovine IFN-alpha ELISA Kit (Sigma-Aldrich, RAB1012) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was collected and serum insulin levels were determined in triplicate using a rat/mouse insulin Enzyme-Linked Sorbent Assay (ELISA) kit from Millipore (EZRMI-13K) according to the instructions of the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... Media aliquots were frozen and subjected to ENG ELISA analysis with the Human Endoglin ELISA kit (Sigma-Aldrich RAB0171) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Monocytes were differentiated into macrophages by addition of 5 ng/ml macrophage colony stimulating factor (Sigma Aldrich) for 6 days ...
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bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-heat shock protein 70 (HSP70) (H5147, Sigma-Aldrich, 1:500), rabbit monoclonal anti-Alix (MCA2493 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-glial fibrially acidic protein (GFAP, 1:100, MA360, Millipore, MA, USA) and rabbit anti-phosphorylated TrkBS478 (1:200 R-1718-50 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule associated protein (MAP2; 1:1,000; Sigma‐Aldrich; St Louis, USA); DAPI (1:2,000 ...
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bioRxiv - Biochemistry 2020Quote: ... ATP11A protein was labeled with mouse anti-Flag antibody (1:2000, Sigma, Germany) and the endoplasmic reticulum (ER ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse anti-glial fibrillary acidic protein (GFAP; 1:500, #G3893; Sigma-Aldrich). The next day sections were washed 3 times with the blocking buffer (15 min each ...
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bioRxiv - Synthetic Biology 2021Quote: ... The enzyme and the expressed proteins were inactivated by adding 15 mM EDTA (Catalog No. E9884, Sigma-Aldrich) at 75°C for 10 minutes (T100 Thermal Cycler ...
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bioRxiv - Microbiology 2022Quote: ... The presence of over-expressed protein was confirmed using monoclonal anti-poly histidine alkaline phosphatase antibody (Sigma-Aldrich) at 1:20,000 dilution.
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bioRxiv - Plant Biology 2022Quote: ... GST-OsIDD12 and GST-OsIDD13 fusion proteins were expressed in Escherichia coli BL21(DE3) Rosetta strain (Novagen, USA). Frozen cells were extracted with 100 μl of extraction buffer containing 50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Neuroscience 2023Quote: ... hERG channel protein was stably expressed in HEK-293 cells purchased from Millipore (Cat. No. CYL3006; Billerica, MA). Assay methods have been previously described (32–34) ...
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bioRxiv - Cell Biology 2024Quote: ... the proteins were expressed in the BL21 strain from Escherichia coli at 37°C in LB medium (Sigma) supplemented with 100 µg/mL ampicillin until OD600 between 0.4 and 0.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was collected and centrifuged at 900g for 5mins and GLP-1 secretion over 1h treatment with GYP was measured using a total GLP-1 ELISA kit (Millipore, UK) according to manufacturer’s specifications.
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bioRxiv - Molecular Biology 2022Quote: ... THP-1-derived macrophages were treated with DMSO or 3 mM Itaconic Acid (Sigma) for 10 hours prior to actinomycin D treatment ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: HMGB1 levels were measured using a commercial kit (HMGB1 ELISA kit A76696, Sigma-Aldrich). The assay was performed as described in the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Active GLP-1 secretion was measured by ELISA (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... NUDT22 were expressed from pET28a(+) (Novagen) vectors ...
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bioRxiv - Biochemistry 2023Quote: ... SAMHD1 was expressed from pET28a(+) (Novagen) vectors expressed with an N-terminal His-tag in E.coli BL21 (DE3 ...
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bioRxiv - Biophysics 2023Quote: The LAF-1 protein coding sequence was ordered as a gBlock Gene Fragment (TM) and inserted into a pET28a(+) vector from Novagen. The LAF-1 mutants E398A (DAAD ...
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bioRxiv - Immunology 2022Quote: ... human bronchoalveloar lavage fluid and mouse serum of animals dosed with human THP were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0751) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Recombinant GST-mm-FMN2-eFSI (GST-FMN2-eFSI) proteins were expressed in Escherichia coli Rosetta bacterial cells (Merck Millipore). Bacteria were cultured in LB medium (100 mg/l ampicillin ...
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bioRxiv - Microbiology 2022Quote: Poly-histidine (His6)-tagged proteins were expressed and purified by affinity chromatography using Ni-NTA agarose beads (Novagen, USA) according to the manufacturer-recommended instructions ...
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bioRxiv - Plant Biology 2019Quote: ... a C-terminal polypeptide encompassing the last 284 amino acids of the PKL protein has been expressed in fusion with a hexahistidine tag in the E.coli strain BL21DE3 Rosetta (Novagen). The protein was purified under denaturing conditions on Ni2+-nitrilotriacetic acid matrix (Ni-NTA Agarose ...
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bioRxiv - Cell Biology 2022Quote: ... and mNedd4△N52 protein fusions were expressed in Rosetta2(DE3)-pLysS cells grown in 2xYT medium (Millipore Sigma, Y2627) at 37°C until the optical density (OD ...
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bioRxiv - Molecular Biology 2023Quote: Uniformly 15N,13C-labelled recombinant Aβ40 was produced as follows: Aβ40 was expressed as a SUMO fusion protein using a modified pET32a vector (Novagen). Protein expression was performed in E ...
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bioRxiv - Cell Biology 2024Quote: ... 3FLAG-6HIS-YFP-tagged proteins expressed from the endogenous locus were immunoprecipitated using anti-FLAG M2 antibodies (F3165, Sigma) (Unnikrishnan et al. ...
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bioRxiv - Physiology 2021Quote: ... and IL-6 (KIT ELISA IL-6-RAB0308-1KT, Sigma-Aldrich) in BALF following the kit instructions ...
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bioRxiv - Neuroscience 2023Quote: The S100B ELISA Kit (#EZHS100B-33K; EMD Millipore, Billerica, MA, US) and Human MMP-14 ELISA Kit (#ab197747 ...
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bioRxiv - Microbiology 2023Quote: ... was frozen for ELISA analysis (IL-1β kit (Sigma-Aldrich RAB1108). MMP9 ELISA ...
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bioRxiv - Neuroscience 2020Quote: Mouse anti-Myelin Basic Protein (MBP-SMI-99, Millipore, reference NE1019, 1/1000 dilution), rat anti-Myelin Basic Protein (BIO-RAD ...
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bioRxiv - Neuroscience 2020Quote: ... Synaptic proteins were detected mouse anti-PSD95 (1:500, MAB1598, Millipore, Burlington, MA, U.S.A.), guinea pig anti-VGLUT1 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... and glial fibrillary acidic protein (mouse anti-GFAP, 1:400, Sigma-Aldrich, cat. #: G3893), and cell nuclei were counterstained using Hoechst (10 µg/mL) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Systems Biology 2019Quote: ... Macrophages were assigned to one of three treatment groups: 1) stimulated with 1 μg/mL LPS (Sigma, L2880) and 20 ng/mL IFNγ (R&D ...
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bioRxiv - Cell Biology 2020Quote: ... and untagged Rab4aΔHVR (Figure 1) were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed ...
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bioRxiv - Molecular Biology 2023Quote: ... The shRNAs expressed by the pKLO.1-puro vector were purchased from Sigma-Aldrich. The sequences for human shDiPRO1 were as follows:
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bioRxiv - Immunology 2020Quote: ... Total IgG response was evaluated by enzyme-linked immunosorbent assays (ELISAs) using 1:5000 dilution of HRP conjugated anti mouse secondary antibody (A4416, Sigma-Aldrich, USA).
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bioRxiv - Immunology 2020Quote: IgG mediated antibody titer of serum samples collected from immunized animals and convalescent patients’ sera were determined by enzyme-linked immunosorbent assay (ELISA)35 using 1:5000 dilution of HRP conjugated anti mouse (A4416, Sigma-Aldrich, USA) and 1:10000 dilution of HRP conjugated anti human secondary antibody (A0170 ...
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bioRxiv - Neuroscience 2021Quote: ... Macrophages were labelled with CTxB-555 and with anti-DAT antibody (1:1000, MAB369, Millipore), mouse anti-NET (1:100) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Microbiology 2023Quote: ... were also challenged with mouse peritoneal macrophages (Nrf2 +/+) or (Nrf2 -/-) pre-treated or not with ML385 at 10µM (Sigma-Aldich) or h-MDM pre-treated with ML385 at 10µM (Sigma-Aldich) ...
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bioRxiv - Immunology 2023Quote: Cryopreserved blood plasma was thawed at room temperature and used for IL17a detection by a commercial mouse IL-17a ELISA Kit (Sigma Aldrich catalog #RAB0263-1KT). The test was performed according to manufacturer guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... All ELISAs were developed using ELISA TMB (Sigma-Aldrich) and absorbance read on a Bio-Tek plate reader ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected with anti-γH2A (1:1000) and mouse anti-Ef1α clone CBP-KK1 (1:25,000; Millipore) primary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...