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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Biochemistry 2019Quote: ... A synthetic gene encoding the KRAB domain (residues 1-71) from ZNF93 (UniProt: P35789) codon-optimized for E.coli was expressed from the pET20 plasmid (Novagen) with N-terminal Twin-StrepII and maltose binding protein (MBP ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-mouse IgG (mouse, A9044, 1:10000,Sigma) at room temperature for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... The interactions between the two labeled proteins were detected using the Duolink in Situ Red Starter Kit Mouse/Rabbit (#DUO92101-1kit, Sigma-Aldrich) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... 1:250 rabbit anti-calcitonin gene-related peptide (CGRP) (Millipore PC205L-100UL), 1:250 rat anti-myelin basin protein (MBP ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified gene drmA was cloned in plasmid pACYCDuet-1 (Sigma-Aldrich: 71147), resulting in a non-tagged protein product ...
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bioRxiv - Microbiology 2022Quote: The C1-07020 gene was cloned into the plasmid pETDuet-1 (Novagen) and then transformed into E ...
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bioRxiv - Biochemistry 2022Quote: ... The csx30 gene was cloned into the pCDFDuet-1 (Novagen, streptomycin resistance) vector with an N-terminal hexahistidine tag and a C-terminal Strep II tag ...
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bioRxiv - Microbiology 2024Quote: Cas1 and cas2/3 genes were subcloned into pRSFDuet-1-MCS1 (Novagen) and pGEX6p-1 (Novagen) ...
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bioRxiv - Immunology 2022Quote: ... The concentration of protein was determined by BCA protein assay kit (Millipore). The heated protein samples were then analyzed by SDS-PAGE using 10% acrylamide/bis-acrylamide and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... and protein concentration was determined by BCA protein assay kit (Sigma, USA). Equal amounts of the extracts were subjected to 8% SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein concentrations were measured using a BCA protein assay kit (Sigma) and normalized when needed by adding extra PE buffer ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Protein concentration was determined by BCA Protein Assay Kit (Sigma-Aldrich, 1001491004) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was determined by BCA assay (Novagen BCA Protein Assay Kit) on a NanoDrop Microvolume Spectrophotometer (Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-HA rabbit antibody (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were estimated using a Lowry assay (Sigma – total protein kit). A3B was detected using an anti-FLAG mouse antibody (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... THP-1 cells were differentiated to macrophages by supplementing the growth medium with 200 nM phorbol myristate acetate (PMA, Sigma) overnight.
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bioRxiv - Microbiology 2020Quote: ... Maturation of THP-1 cells into macrophages was induced by adding 200 nM PMA (phorbol 12-myristate 13-acetate; Sigma) in cell culture medium for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: THP-1 was differentiated into macrophages by the treatment with 100 ng/mL PMA (Sigma-Aldrich, St Louis, MO, USA) for 48 hours ...
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bioRxiv - Microbiology 2019Quote: ... supernatants were collected and analyzed for total GLP-1 levels by ELISA (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with the Gene Elute Plasmid Miniprep kit (Sigma, St. Louis, USA) and sent for sequencing to two different companies (EuroFins Genomics ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in 125 μl of lysis buffer provided with a CAT ELISA kit (Sigma). CAT expression and Renilla luciferase activities were measured and the ratio was calculated.
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Insulin concentration of the supernatants was measured with a mouse/rat insulin ELISA (EZRMI-13K, EMD Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Plant Biology 2023Quote: ... Plant Total Protein Extraction Kit (Sigma-Aldrich) was used ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Microbiology 2022Quote: ... The CF640-labelled ACE2 at 12–14 different concentrations (200 nM to 13 pM in PBS supplemented with bovine serum albumin at 1 g/l) per measurement were incubated with expressed yeast aliquots and 1 nM bilirubin (Sigma-Aldrich, Cat# 14370-1G) and analysed by using FACS S3e Cell Sorter device (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-phospho-S345 mouse MLKL (Millipore MABC158; clone 7C6.1; 1:2000/1:200), rabbit anti-phospho-S345 mouse MLKL (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Biophysics 2022Quote: ... and mouse monoclonal primary antibody against myelin basic protein antibody (Millipore) at 1:200 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... mouse monoclonal anti-glial fibrillary acidic protein (GFAP, #G3893, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Cell Biology 2023Quote: ... Cenexin rabbit antibody (Protein tech) and centrin mouse antibody (EMD Millipore) diluted 1:1000 for centrin and 1:200 for other centrosomal proteins in 1% BSA in PBS for 1.5 hours at room temperature ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Biophysics 2021Quote: Recombinant HSA expressed in Pichia pastoris was purchased from Sigma-Aldrich (#A7736 ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2020Quote: ... CtXPB was expressed in Rosetta™ 2 (DE3) cells (Merck Millipore), ctXPD was expressed in ArcticExpress (DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... GST-VAPB was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG for 4 h ...
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bioRxiv - Biochemistry 2022Quote: ... UBR5 and UBR5 mutants were expressed in Sf9 insect cells (Novagen) using the Bac-to-Bac baculovirus expression system (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: The constitutively active Ran mutant was expressed in BL21-pLysS (Novagen) E ...
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bioRxiv - Biochemistry 2023Quote: ... SUN1 and KASH6 were co-expressed in BL21 (DE3) cells (Novagen) in 2xYT media ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were stained overnight at 4°C with primary antibody (Mouse anti-γH2AX antibody; Millipore Sigma #05-636, 1:300) diluted in blocking solution ...
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bioRxiv - Developmental Biology 2021Quote: ... 30 μl of Protein G coated Bio-Adembeads (Ademtech) were incubated with either 1 μg of mouse monoclonal anti-FLAG antibody (F3165, Sigma), or goat anti-HA antibody as mock (sc-805 ...