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Citations for Millipore Sigma :
401 - 450 of 2366 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... the caudal epididymis was dissected and the epididymal sperm was collected on a needle and capacitated in EmbryoMax Human Tubal Fluid media (HTF, EMD Millipore) for 30 minutes at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... non-specific binding was blocked with 0.2% gelatin from cold water fish skin (#G7765 Sigma-Aldrich Chimie, Lyon) in TS for 30min ...
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bioRxiv - Immunology 2021Quote: ... for 10 mins at room temperature and blocked in PBS containing 5% fish gelatin (Sigma Aldrich; Cat.# G7765), 1% BSA (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... fish were anesthetized by immersing in fresh water containing 200 mg/L tricaine methanesulfonate (MS222; Sigma-Aldrich, A5040) and set in a device for physical restraint ...
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bioRxiv - Biochemistry 2020Quote: ... the probes (either T30 or (C4G2)5) were hybridized in FISH buffer with 10% dextran sulphate (Sigma Aldrich) for 3 h at 42°C ...
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bioRxiv - Neuroscience 2021Quote: ... the animal was anaesthetized in fish facility water containing 0.02 % (w/v) tricaine mesylate (MS-222, Sigma-Aldrich), and embedded in 2 % low melting-point agarose ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Membranes were incubated with primary antibodies (Supplemental Information) diluted 1:1000 in 1% fish gelatin (Sigma Aldrich, G7041)/TBST overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... For blocking, samples were incubated in PB buffer (0.5% skim milk powder, 0.25% fish skin gelatin (Sigma-Aldrich), 0.5% Triton-X100 in HBS (20 mM HEPES pH 7.2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... fish were immersed in fresh Tricaine (0.016%w/v; 3-amino benzoic acid ethyl ester; Sigma A-5040) in system water buffered with NaHCO3 to pH 7.5 until motionless ...
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bioRxiv - Developmental Biology 2020Quote: ... Fish were stained overnight at room temperature (RT) with a solution of 0.1% Alcian blue 8GX (Sigma Aldrich) w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Anaesthesia was administered in E2 medium (embryos) or fish tank water (adults) using 0.02% pH7.0 buffered Tricaine MS-222 (Sigma). The hai1a/ddf alleles used were hai1ahi2217 ...
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bioRxiv - Microbiology 2022Quote: ... Euthanasia was carried out by bathing fish in tricaine at concentration of 300 mg/L (MS222, Sigma-Aldrich). Dead or euthanized fish were examined for the presence of bacteria in organs including the spleen ...
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bioRxiv - Genetics 2023Quote: ... Labelled double-stranded and ssRNA FISH probes were then precipitated in presence of 0.3M sodium acetate (Sigma, #71196) and 0.5M ammonium acetate (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... and then blocked in 0.5% Triton-X-100 and 1% fish gelatine in PBS (Sigma-Aldrich, cat.# G7765) also for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the fish water was pipetted out and replaced with 0.5 ml d-amphetamine hemisulfate solution (Sigma Aldrich, MO). The drug was allowed to seep through the agarose drop for ∼5 mins ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... fish were side-embedded in the imaging dish using 500 µl 0.75 % low-melting temperature agarose (Sigma, A4018). Widefield imaging was done using the Zeiss Axio Observer fluorescence microscope (LD A-Plan 10x objective ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were treated with blocking buffer (TEM-BB) composed of 1% fish skin gelatin (Sigma-Aldrich / Merck) dissolved in 0.1M HEPES for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... blocking was performed in PBS + 0.1 % (v/v) acetylated BSA (BSA-c, Aurion) + 0.5 % (w/v) cold fish-skin gelatine (FSG, Sigma) for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... Sperm-oocyte complexes were then washed four times in M2 medium and incubated in 30 µL KSOM medium (Millipore, MR-101-D) at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: Age- and sex-matched adult zebrafish fish were sacrificed in 200 mg/L of MS-222 (Sigma, MO, USA) and portions of each tissue (gonads ...
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bioRxiv - Cell Biology 2020Quote: Age- and sex-matched adult zebrafish fish were sacrificed in 200 mg/L of MS-222 (Sigma, MO, USA) and portions of each tissue (gonads and gut ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Filters for counts of reporter deposition coupled with fluorescence in situ hybridization (CARD-FISH) were embedded in 0.1% low-melting agarose (Sigma), dried at 37 °C for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Fish with successful intravenous transplants were anesthetized with Tricaine-S and embedded in 1% low-melt agarose (Sigma A9045) in E3 containing 0.28ug/mL Tricaine-S in a glass-bottom square-well 96-well plate (Arrayit 96-well Microplate SuperClean ...
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bioRxiv - Plant Biology 2020Quote: ... epitopes were blocked with 0.1 M PBS / 1% Cold Water Fish Skin Gelatin / 0.2% BSA with 3% sucrose (all chemicals from Sigma Aldrich), incubated with the primary antibody anti-GFP (1:250 ...
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bioRxiv - Neuroscience 2021Quote: ... glucose solution at 37 °C for 20 min and rinsed five times with washing buffer (PBS −/−with 0.2% fish skin gelatin (G7765, Sigma)) ...
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bioRxiv - Cancer Biology 2021Quote: ... Paraformaldehyde solution for 15 min after which cells were permeabilized with 0.2 % (v/v) Triton-X solution and blocked with 0.2 % (v/v) Fish Skin Gelatin (FSG) (Sigma–Aldrich) for 1 hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5% fish skin gelatin (Sigma-Aldrich) for 20 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads (20 μl of the original slurry) were pre-blocked for one hour at room temperature in PBS containing 0.5 % cold water fish gelatin (Sigma) and 0.02 % Tween-20 and washed twice with CoIP buffer ...
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bioRxiv - Immunology 2020Quote: ... The separated proteins were transferred to PVDF membranes and blocked in PBS containing 0.2% fish skin gelatin (Sigma-Aldrich) and 0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2020Quote: ... then permeabilized using PBS/0.1% Triton X-100 and eventually blocked with PBS/0.2% Fish Skin Gelatin (Sigma, Germany). Primary antibodies were diluted into 5% BSA in PBS and incubated onto coverslips for 1 h at 37 °C in a wet chamber ...
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bioRxiv - Neuroscience 2021Quote: ... Fish were deeply anesthetized with 0.2% 3-aminobenzoic ethyl ester (MS-222; Sigma-Aldrich, St. Louis, MO; RRID: SCR_008988) in water just before surgery or tissue preparation.
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bioRxiv - Immunology 2022Quote: ... fish were sampled after the experiments and euthanized by immersion in 0.25 mg/ml of buffered MS-222 (Sigma). Individual brains were dissected in PBS and flash frozen in liquid nitrogen and processed immediately or stored at -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were permeabilised in 0.2% TX-100 in PBS for 30 mins and epitopes blocked with 1% fish-skin gelatin (Sigma) in PBS for 1 hour ...
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bioRxiv - Cell Biology 2020Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 3 min and blocked in PBG (PBS, 0.045% cold fish gelatin (Sigma), and 0.5% BSA) ...
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bioRxiv - Microbiology 2020Quote: ... Randomly chosen fish were sacrificed by an overdose of filtered tricaine methane sulfonate solution (tricaine, Sigma, 300 mg/L). Whole fish were mechanically disrupted in Lysing Matrix tubes containing 1 ml of sterile water and 425-600 µm glass beads (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed 3 times in PBS and blocked in PBS supplemented with 0.5 % fish skin gelatin (Sigma Aldrich) for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: ... cat#2851S from Cell Signalling Technology) after blocking with 0.5% BSA and 0.2% Gelatin from cold water fish skin (cat#G7765 from Sigma Aldrich) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Fish were then anesthetized by applying 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521) to the fish mounted in agarose ...
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bioRxiv - Microbiology 2023Quote: ... and incubated in blocking buffer (2,8 mM KH2PO4, 7,2 mM K2HPO4, 5% goat serum [Gibco, Billings, MT], 5% glycerol, 1% cold water fish gelatin [Sigma, St Louis ...
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bioRxiv - Neuroscience 2023Quote: ... a fish was humanely killed by immersing in 0.1% tricaine MS-222 (E10521; Sigma-Aldrich, St. Louis, MO, USA) in E3 medium ...
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bioRxiv - Cancer Biology 2023Quote: Embryos were initially maintained at 28 °C at maximum density of 50 embryos per Petri dish in fish water supplemented with 0.0002% methylene blue (Sigma) as an antifungal agent ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were washed before blocking in PBS +0.01% Triton X-100 and 0.2% fish skin gelatin (Sigma-Aldrich - G7765) for 1 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were first slightly anesthetized with a bath in tricaine (160 mg/L in fish water; A5040, Sigma-Aldrich) to reduce movements ...
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bioRxiv - Molecular Biology 2024Quote: ... The blot was blocked for one hour and hybridized with primary antibody overnight in 0.5% fish gelatin (G7041, Sigma) dissolved in TBST (0.05% tween) ...
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bioRxiv - Microbiology 2024Quote: ... Liver and spleen samples from experimentally infected fish for 3 days were fixed in 4% paraformaldehyde (PFA, Sigma, USA), embedded in paraffin and sectioned (4 μm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... sperm and cumulus cells were mechanically removed by repeated pipetting and eggs/zygotes were transferred to KSOM medium (Millipore Sigma, MR-106-D) for further culture ...
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bioRxiv - Cancer Biology 2021Quote: ... washed twice in PBS, blocked for 1 h in PFS buffer (PBS, 0.7% w/v fish skin gelatin (Sigma-Aldrich), 0.5% saponin (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The fish were anesthesized using 32.5 μl from a 10% stock solution of ethyl-p-aminobenzoate (Benzocaine E1501, Sigma-Aldrich) dissolved in 100% ethanol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: The left second gill arch of control and 7-day exposed fish was collected for histological analysis and placed in 4% aqueous formaldehyde (Sigma). The fixation period lasted for 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... wells were washed with embryo media before the fish were incubated in media containing 0.01% 2-[4-(dimethylamino) styryl]-1-ethylpyridinium iodide (DASPEI, Sigma-Aldrich) to stain for neuromast mitochondrial activity for 20 minutes ...