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Citations for Millipore Sigma :
501 - 550 of 2366 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... cldnB:lyn2GFP embryos were dechorionated at 24 hpf and incubated in the fish water supplemented with either Rockout (final concentration 50 μM, Millipore Sigma, catalog # 555553), blebbistatin (final concentration 1 μM ...
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bioRxiv - Developmental Biology 2023Quote: ... TgBAC(cxcr4b:LexPR-LexOP:Arhgef11-T2A-H2A-TagBFP)] and control sibling embryos were dechorionated at 24 hpf and incubated in fish water with either RU-486 (final concentration 1 μM, Millipore Sigma, catalog # M8046) or ethanol until 48 hpf ...
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Can Intestinal Absorption of Dietary Protein Be Improved Through Early Exposure to Plant-Based Diet?bioRxiv - Zoology 2020Quote: ... five fish in each tank were euthanized with MS-222 and intestines were harvested and stored in RNALater (Sigma-Aldrich, St. Louis, MO, USA) for future analysis of gene expression and an assessment of intestinal health.
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bioRxiv - Cell Biology 2023Quote: ... After blocking for 1 hour in blocking buffer (for Fig. 1D: 5% Protifar; for Suppl. Fig. 2B: 0.2% v/v Fish skin gelatin (Sigma–Aldrich, St. Louis, MO, USA)+ 0.1% v/v Tween–20 ...
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bioRxiv - Biophysics 2020Quote: ... Calf thymus DNA (CT-DNA, Sigma) was dissolved in the same cacodylate buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the specimens were transported alive to the laboratory in aerated containers to minimize fish discomfort and euthanized immediately upon arrival with an overdose of tricaine mesylate (400 ppm of MS-222; Sigma-Aldrich, St. Louis, MO, USA) with sodium bicarbonate (1:2 ...
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bioRxiv - Developmental Biology 2023Quote: ... or 96 hpf and fixed in groups of 10-15 fish per 1.5 mL Eppendorf® Safe-Lock microcentrifuge tube (Millipore Sigma, Cat. No. T9661-500EA) in 4% paraformaldehyde (PFA ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish embryos and larvae were collected at 72 hpf and fixed in groups of 10-15 fish per 1.5 mL Eppendorf® Safe-Lock microcentrifuge tube (Millipore Sigma, Cat. No. T9661-500EA) in 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2022Quote: ... herring testis DNA (HT-DNA) (Sigma-Aldrich), a 24 bp dsDNA (P24 ...
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bioRxiv - Physiology 2021Quote: ... Canopy cover above each sampled mound was quantified using a hemispherical fish-eye lens (Canon EOS 70D camera, Sigma 4.5mm f/1:2.8 Circular Fisheye Lens). For large mounds the image was taken directly above the apex of the mound ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA oligos (Sigma-Aldrich or Integrated DNA Technologies) were annealed and ligated into AarI (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Calf thymus DNA (CT-DNA) was purchased from Millipore, Merck ...
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bioRxiv - Biochemistry 2020Quote: DNA primers (Sigma) were radiolabeled at the 5’-end with 32P using the manufacturer recommended protocol for T4 PNK (New England Biolabs ...
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bioRxiv - Biochemistry 2020Quote: DNA (Sigma-Aldrich) and RNA (Dharmacon ...
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bioRxiv - Microbiology 2020Quote: ... Integrated viral DNA was quantified by first pre-amplifying the DNA using AccuTaq™ LA DNA Polymerase (Sigma) using the following primers ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted genomic DNA (GenElute Bacterial Genomic DNA kit, Sigma-Aldrich), quantified it (Qubit HS dsDNA assay ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was purified using GenElute Bacterial Genomic DNA Kit (Sigma) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... Calf thymus DNA (CT DNA) was purchased from Sigma (#D1501-100MG).
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bioRxiv - Molecular Biology 2020Quote: ... DNA was isolated using GenElute Plant Genomic DNA Miniprep Kits (Sigma), and 1 μL of DNA was used in a PCR reaction using 0.3 μΜ primers and 5U of MyTaq DNA polymerase (Bioline).
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was isolated using the GenElute DNA extraction kit (Sigma) with inclusion of lysozyme and RNAase.
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bioRxiv - Neuroscience 2022Quote: ... Taq DNA polymerase and phusion DNA polymerasewere purchased from Sigma (USA). DNA ladders ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was isolated using the GenElute DNA extraction kit (Sigma) with inclusion of lysozyme and RNAase ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We extracted genomic DNA (GenElute Bacterial Genomic DNA kit, Sigma-Aldrich), quantified it (Qubit HS dsDNA assay ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted genomic DNA (GenElute Bacterial Genomic DNA kit, Sigma-Aldrich) and quantified it using the Qubit HS dsDNA assay kit (Invitrogen) ...
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bioRxiv - Genetics 2019Quote: ... Minigenes were constructed by DNA assembly of PCR fragments that were amplified from HEK293T genomic DNA using KOD Hot Start DNA polymerase (Novagen). For the wild-type minigene ...
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bioRxiv - Microbiology 2019Quote: ... DNA fragments were amplified with either KOD Hot Start DNA Polymerase (Novagen) or standard Taq polymerase (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... on genomic DNA prepared with GenElute Mammalian Genomic DNA miniprep (Sigma-Aldrich). The sequences of oligonucleotides are given below.
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bioRxiv - Genomics 2021Quote: DNA was extracted with the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The DNA was extracted using GeneElute bacterial DNA kit (NA2110, Sigma-Aldrich) by following the manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... chromosomal DNA was extracted using the GenElute Bacterial Genomic DNA Kit (Sigma). One ng of the chromosomal DNA obtained was used in a library preparation using the Nextera XT library preparation kit (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted using the GenElute – Plant Genomic DNA Miniprep Kit (Sigma), samples were cleaned by DNAse (as described above) ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was complexed with X-tremeGENE 9 DNA Transfection Reagent (Sigma, 6365779001) in OptiMEM according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: calf thymus DNA and supercoiled plasmid pUC19 DNA were obtained from Sigma Chemical Company ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using the GenEluteTM Bacterial Genomic DNA Kit (Sigma-Aldrich) according to the supplied protocol ...
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bioRxiv - Immunology 2019Quote: ... Sheared CT DNA (Sigma) and 2’3’ cGAMP (Invivogen ...
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bioRxiv - Biochemistry 2019Quote: ... herring testes DNA (Sigma) was also run on each plate to ensure consistency of data.
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bioRxiv - Immunology 2023Quote: ... purified DNA (Sigma-Aldrich), actin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... the total DNA methylation signature was evaluated in 100 ng of DNA using the commercial kit (Imprint Methylated DNA Quantification kit, Sigma-Aldrich), according to the manufacturer’s recommendations.
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bioRxiv - Neuroscience 2021Quote: ... and stuffer plasmid DNA (to adjust the amount of total DNA to 1 μg) using X-tremeGENE 9 DNA transfection reagent (#6365779001, Sigma-Aldrich). For controls ...
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bioRxiv - Cell Biology 2020Quote: ... The DNA was extracted using a DNA purification kit (Merck Millipore, Darmstadt, Germany). Anti-RNA polymerase II antibody and normal mouse IgG were used as specific antibodies and the negative control ...
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bioRxiv - Cell Biology 2020Quote: ... DNA content was calculated from a standard curve (calf thymus DNA; Sigma-Aldrich) made in neutralized papain buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and genomic DNA was isolated using the GenElute Plant Gemomic DNA Kit (Sigma) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Genomic DNA was extracted using the GeneElute Mammalian Genomic DNA Miniprep Kit (Sigma). Sonication with the Covaris M220 was used to shear genomic DNA randomly and DNA linkers were ligated to the sheared DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... Extracted DNA was processed using GenElute™ Mammalian Genomic DNA Miniprep Kit (Sigma), including the optional RNase A digest ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.8 µL T4 DNA ligase buffer and 1 µL T4 DNA ligase (Sigma) were added ...
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bioRxiv - Cancer Biology 2019Quote: ... Genomic DNA was extracted with the GenElute Mammalian genomic DNA miniprep kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... genomic DNA was prepared using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich) according to the manufacturer’s guidelines and sequenced at the Microbial Genome Sequencing Center (MiGS ...
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bioRxiv - Genomics 2020Quote: Genomic DNA isolated using GenEluteTM Bacterial Genomic DNA Kit (NA2120-1KT, Sigma Aldrich) were subjected to Nanopore sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... and PCR on extracted DNA (GenElute Mammalian Genomic DNA Miniprep Kit; Sigma-Aldrich). Selected clones were finally adapted to growth in suspension in Expi293 Expression Medium (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... and DNA fragments were generated using KOD Hot Start DNA polymerase (EMD Millipore). Yeast transformation was performed using the Frozen-EZ Yeast Transformation II Kit (Zymo Research) ...