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Citations for Millipore Sigma :
351 - 400 of 2366 citations for Fish Sperm DNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... larvae were transferred to fish water supplemented with N-phenylthiourea (PTU; Millipore Sigma, Catalog #P7629-25G) between 18-22 hours post-fertilization to inhibit melanin formation ...
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bioRxiv - Neuroscience 2020Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Cell Biology 2021Quote: ... Lungs designated for FISH were fixed with 4% (w/v) PFA (Sigma-Aldrich, Oakville, ON, Canada). In both instances ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were anesthetized in a solution of 300 mg/L tricaine methanesulfonate (MS-222) (Sigma-Aldrich) and positioned on a plastic platform in a recording chamber filled with fresh water (Fig ...
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bioRxiv - Cell Biology 2021Quote: Probes for immuno-FISH on mouse cells and tissues were digoxigenin-labeled (Digoxigenin-11-UTP, Sigma) by nick translation from the following BAC or FOSMID clones covering genes loci ...
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bioRxiv - Neuroscience 2022Quote: ... we used a pronase solution (50 mg/ml in fish water for 5 min, Sigma-Aldrich) to dissolve the chorion prior to 24 hours post fertilization (hpf) ...
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bioRxiv - Developmental Biology 2022Quote: ... we applied amputation injury and then bathed fish in 5 mM metronidazole (Mtz, Sigma-Aldrich, M1547) for 3 successive days from 3 to 5 dpa with fish water being changed daily14 ...
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bioRxiv - Neuroscience 2022Quote: ... for 15 min at RT and subsequently blocked with 0.2% cold fish gelatin (Sigma-Aldrich G7765), 1% goat serum (Thermo Fisher Scientific 16210064 ...
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bioRxiv - Biophysics 2022Quote: ... a glass coverslip (CS) was incubated with 1% gelatin (gelatin from cold water fish skin, Sigma) and put at 37°C in the incubator for 1h ...
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bioRxiv - Genetics 2023Quote: ... Fish were anaesthetised for live imaging using Tricaine methanesulfonate (3-amino benzoic acidethylester; Sigma Aldrich, E10521) at a final concentration of 0.016% in E3 embryo medium (5 mM NaCl ...
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bioRxiv - Developmental Biology 2023Quote: ... juvenile fish of 1-2 cm in length were anesthetized in 0.1% (w/v) tricaine (Sigma). Images were acquired on a fluorescent stereomicorcope (Lumar.V12 ...
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bioRxiv - Biochemistry 2023Quote: ... Larvae were fed a mixture of ground fish food (Tetramin) and 1% yeast extract (Sigma-Aldrich). Adult mosquitoes have access to water and 10% sucrose solutions ...
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bioRxiv - Immunology 2023Quote: ... we immersed euthanized fish into 0.04% solution of Alizarin red powder (Sigma Aldrich 130-22-3) dissolved in RO water in a 6-well plat for 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... Fish heads were subsequently removed and fixed overnight in 10% neutral buffered formalin solution (Sigma, USA), thoroughly rinsed with PBS during 5 min for at least 3 times ...
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bioRxiv - Developmental Biology 2023Quote: ... but those for detection of phosphorylated proteins were blocked overnight in 5% gel fish (Sigma, #G7765). Blots were probed with antibodies listed in Suppl ...
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bioRxiv - Immunology 2023Quote: ... Whole fly guts and fish larval intestines were mounted on microscope slides using FlouromountTM (Sigma; F4680) and visualized with a spinning-disk confocal microscope (Olympus IX-81 motorised microscope base with Yokagawa CSU 10 spinning-disk scan-head).
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bioRxiv - Neuroscience 2023Quote: ... Then the fish was mounted on a 3D-printed holder in 3% low-melting agarose (Sigma), all within a petri dish (Figure 1d) ...
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bioRxiv - Zoology 2024Quote: ... several fish were anesthetized with tricaine methane sulfonate (MS-222; 0.02%, Sigma-Aldrich, St. Louis, MO), and their gonads were dissected ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking and permeabilization steps were done with 1% BSA and 0.5 % cold fish gelatin (Sigma-Aldrich) dissolved in Tris + 0.1% Triton solution ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We weighed paired testes and seminal vesicles relative to body weight and isolated sperm by dicing the caudal epididymides in 1 mL of Dulbecco’s PBS (Sigma) followed by a 10 min incubation at 37°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sperm was then released by gently pressing the belly and collected in a micro capillary tube (Sigma-Aldrich, P0674). Collected sperm was added to microtubes containing 20μl premix solution ...
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bioRxiv - Microbiology 2023Quote: ... 0.05 % w/v deoxyribonucleic acid from herring sperm and 0.15 % w/v NH4NO3 adjusted to pH 7.0 with NaOH (Sigma Aldrich) and supplemented with 1% glucose ...
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Impact of light pollution at night on male reproductive success in Japanese medaka (Oryzias latipes)bioRxiv - Zoology 2023Quote: ... before the sperm was extracted by homogenizing the testes in 120 μL Hank’s balanced salt solution (HBSS, 287 mOsmol/kg, Sigma) with forceps ...
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bioRxiv - Cell Biology 2020Quote: Age- and sex-matched fish were sacrificed in 200 mg/L of MS-222 (Sigma, MO, USA) and portions of each tissue (gonads ...
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bioRxiv - Developmental Biology 2021Quote: ... 48-or 72-hour fish were embedded into 1% low melting agarose (Sigma-Aldrich-Aldrich, A9414-250G) supplemented with Mesab in 35mm glass bottom confocal dishes (Cellvis ...
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bioRxiv - Molecular Biology 2021Quote: ... Fish were anesthetized by submersion bath into tricaine methanesulfonate (MS222, Sigma Aldrich, St. Louis, Missouri, United States) solution in seawater (15-30 mg/L ...
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bioRxiv - Developmental Biology 2022Quote: ... Caudal fin amputations were performed in fish anaesthetized with buffered 160 mg/mL MS-222 (Sigma, E10521), using a sterile scalpel to remove approximately one half of the fin ...
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bioRxiv - Molecular Biology 2021Quote: ... were blocked for 1 hr at 4°C in Buffer BC150 containing 1% fish skin gelatine (Sigma) and 0.2 mg/ml BSA (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... These experiments in the MCU-overexpressing fish were additionally performed in the presence of Ru360 (Millipore, 557440) at 100 μM ...
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bioRxiv - Developmental Biology 2021Quote: ... was dissolved in ethanol and added to the fish water with fatty acid free BSA (SIGMA, A8806), at 6-20 μg/ml concentration.
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bioRxiv - Microbiology 2021Quote: ... in PBS for 10 min then incubated in blocking buffer (0.1% fish gelatin [Sigma Aldrich] in PBS) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: Fish skin tissue was enzymatically dissociated with Liberase TM (Sigma-Aldrich LIBTM-RO, 0.25mg/ml in dPBS) at 32°C for 20 minutes followed by manual titration with a glass pipette for 2 minutes ...
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bioRxiv - Developmental Biology 2022Quote: Fish 3 – 45dpf (days post-fertilization) old were killed by prolonged anesthesia in 0.02% Tricaine solution (Sigma) on ice ...
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bioRxiv - Neuroscience 2019Quote: Anesthesia was initiated by soaking the fish for approximatively 90 seconds in water containing 0.01% MS222 (Sigma). They were then transferred into a water solution of 0.005% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the fish were immersed in fresh buffered Tricaine (3-amino benzoic acid ethyl ester; Sigma A-5040) diluted in system water (0.016%w/v ...
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bioRxiv - Developmental Biology 2020Quote: ... Animals were then blocked (1X PBS, 0.3% Triton X-100, 0.3g/10ml BSA [Jackson], 0.45% fish gelatin [Sigma]) for at least 4 hr ...
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bioRxiv - Immunology 2021Quote: ... Adult fish were first anaesthetised in system water supplemented with MS-222 (168 mgs/mL; Sigma E10505). They were then placed in the lid of a petri dish with their right side facing up ...
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bioRxiv - Developmental Biology 2021Quote: Adult fish (between 3-6 mpf) were anesthetized by immersion into 0.04% tricaine (Sigma, St Louis, MO) and the AF were carefully detached using surgical blade and forceps ...
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bioRxiv - Neuroscience 2022Quote: ... we used a concentration of 60 µg/ml tricaine in filtered fish water (MS-222 Sigma-Aldrich). We came to this concentration through extensive testing of a wide range of concentrations ...
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bioRxiv - Cancer Biology 2022Quote: ... Blocking was performed using 0.5% BSA + 0.2% gelatin from cold water fish skin (Sigma, cat no. G7765) in PBS for 1 h at RT ...
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bioRxiv - Zoology 2023Quote: ... fish were euthanised with an overdose of MS222 (tricaine methanesulfonate, 0.03% solution buffered in seawater; Sigma-Aldrich).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5% Bovine Serum Albumin (BSA; fraction V; 8076.3; Carl Roth) and 0.2% fish gelatine (G7765; Sigma-Aldrich) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: Uninjured and light-lesioned Tg(pcna:EGFP);Tg(gfap:nls-mCherry) fish were exposed to an overdose (0.2%) of MS-222 (Sigma) (Brand et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Tissues were incubated in antibody blocking solution with a solution containing 2% fish gelatin (Sigma Aldrich #7765), 5% donkey serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL of fresh sperm was mixed with a drop of HEPES-HTF medium containing 10% (w/v) polyvinylpyrrolidone (PVP, Sigma). Piezo pulses were used to separate the sperm head from the tail ...
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bioRxiv - Genetics 2020Quote: ... An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma, Cat# P5288) in water (Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... antibody-bound fraction was immunoprecipitated for 90 minutes at 4°C using 25 µL slurry salmon sperm protein A agarose beads (Millipore). Unbound proteins were removed by washing the beads 5 times in wash buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Genetics 2020Quote: ... The sperm sample was then reconstituted in high calcium HTF plus 10% PVP media (Sigma-Aldrich, St. Louis, MO, USA). Individual sperm were sorted into separate droplets of HTF with a piezo micromanipulator (Eppendorf ...
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bioRxiv - Physiology 2020Quote: ... Spermatozoa were activated by mixing 1 µL of diluted sperm (1:4 with modified Leibovitz) with 20 µL of seawater with 30% bovine serum albumin (BSA, Sigma, Spain). One µL of activated sperm was pipetted into an ISAS R2C10 counting chamber (Proiser R+D ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... eggs were washed to remove excess sperm and then cultured in 10 μL droplets of KSOM (Zenith biotech) supplemented with 1 mg/mL BSA (Sigma-Aldrich) and overlaid with embryo-tested light mineral oil (Millipore ...