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Citations for Millipore Sigma :
5001 - 5050 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... the samples were first homogenized and hydrolyzed in concentrated hydrochloric acid (HCl) at 120°C for 3 hours before being quantified using hydroxyproline assay kit (Sigma-Aldrich). A microplate reader (Promega GloMax ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK 293T cells (ATCC) were cultured at 37 °C with 5% CO2 in DMEM medium supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), 2 mM L-glutamine and 500 units mL−1 penicillin and streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: HEK 293T and HeLa cells were cultured at 37 °C with 5% CO2 in DMEM medium supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), 2 mM L-glutamine and 500 units mL−1 penicillin and streptomycin ...
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bioRxiv - Biophysics 2023Quote: ... Cells are grown in LB + 1:2000 kanamycin (50 mg/ml stock) overnight at 30 °C and diluted next day in M9 succinate + amino acids (RPMI 1640 diluted 1x, R7131; Sigma-Aldrich;) + Pluronics (F-108 ...
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bioRxiv - Biochemistry 2023Quote: ... and the remaining lysate was subjected to immunoprecipitation (IP) overnight at 4°C using either Anti-V5 Agarose Affinity Gel beads (Sigma, A7345) or Anti-FLAG M2 magnetic beads (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... 4-OHT was redissolved in ethanol by shaking at 37°C and filtered corn oil was added (Sigma, C8267, 150 uL). Ethanol was then evaporated by vacuum under centrifugation to give a final concentration of 10 mg/mL ...
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bioRxiv - Cancer Biology 2023Quote: MFPs were fixed in 10% neutral buffered formalin (NBF) for 24 hr at 4 °C and submerged in 30% sucrose (Sigma-Aldrich) for 48 hr at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Stationary phase cultures (37°C, 18-24 h, with shaking) were washed twice with sterile phosphate buffered saline (PBS; D8537, Sigma-Aldrich) and diluted to 5 x 105 CFU.mL−1 for experiments ...
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bioRxiv - Immunology 2023Quote: ... and maintained at 37°C with 5% CO2 in complete RPMI in the presence of 5% human AB serum (Sigma-Aldrich) for differentiation into MDMs for 10-14 day (Pierini et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... 95°C and run on 10% SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran Western blotting membranes – Sigma Aldrich) to validate protein quality and specificity.
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bioRxiv - Neuroscience 2023Quote: Reverse transcription of the RNA-IP samples and of 1% Input was carried out at 37° C by random primers (Sigma-Aldrich) and M-MLV Transcriptase (Invitrogen) ...
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bioRxiv - Neuroscience 2023Quote: ... ganglia were treated for 30 minutes at 37 °C with Collagenase II (1.5 mg/ml; Sigma-Aldrich, St. Louis, MO, USA). The enzymatic digestion was stopped by washing the DRGs with 1ml of DMEM low glucose (Gibco ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α by heat shock at 42°C for 30 seconds and 45 minutes outgrowth in 1mL Luria Broth (LB Broth, Sigma-Aldrich). 100μL were then plated on agar plates supplemented with 100μg/mL ampicillin (Ampicillin ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies were then incubated overnight at 4°C in 0.1% BSA in 1x PBS in the following dilutions: 1:1000 RYBP (Millipore Sigma HPA053357), 1:500 Cytokeratin-7 (Millipore Sigma MABT1490) ...
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bioRxiv - Microbiology 2024Quote: ... at low voltage (50 V) and constant current in a 4°C until the protein marker (BLUeye Prestained Protein Ladder, Sigma-Aldrich) front reached the end of the gel ...
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bioRxiv - Pathology 2024Quote: ... or BMS-470539 were delivered topically by pipette into the wound cavity immediately after wounding (40 μl in a 30% Pluronic F-127 gel (liquid at 4°C, but solidifies at room temperature; Sigma Aldrich). Wound areas were calculated using ImageJ software.
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bioRxiv - Neuroscience 2024Quote: ... and maintained in the cell culture incubator at 37°C with humidified 5% CO2 and subcultured when they reached 80%–90% confluence using Trypsin (T2610-100ML, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... at a concentration of 20 mg/mL for 60 min at 37°C and 20 mL of N-tert-butyldimethylsilyl-N-Methyltrifluoracetamide with 1% tert-Butyldimethylchlorosilane (TBDMS) (#375934, Sigma-Aldrich) for 60 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the cultures in the logarithmic growth phase were centrifuged at 5,500 × g for 10 min at 4°C (SIGMA, 3K30 centrifuge). The pelleted cells were then separated from the supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... Ubiquitin was expressed as an untagged protein and then purified and labelled with Cy3 as per ref53.TRIB1 C-terminus (345-372) was first purified using Ni2+ affinity resin (His-Select resin (Sigma-Aldrich) before being dialysed into PBS pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... washed 3x 15 minutes in DEPC-PBS and incubated at 37°C for 15 minutes in proteinase K solution (Sigma-Aldrich; 1.45 μg proteinase K per milliliter of 100 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Cell Biology 2024Quote: ... and samples rocked at 4°C for 30 minutes before centrifugation and incubation of supernatants with glutathione beads (GE17-0756-01, Sigma Aldrich) for 2 hours rocking at room temperature to capture the GST-D4H*-mCherry protein ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed for 1 day in 4% PFA at 4°C before transferring to 30% sucrose (Millipore Sigma) in PBS at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... Unbound dye was removed using dialysis against a 1000-fold excess of PBS or Broccoli aptamer buffer at 4 °C overnight with the Pur-A-LyzerTM Mini 6000 Dialysis Kit (Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... Actin was then cleaved off the beads overnight at 4 °C using 5 μg/ml TLCK treated chymotrypsin (SIGMA: C3142-25MG) which was then inactivated by 1mM PMSF ...
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bioRxiv - Systems Biology 2024Quote: ... pre-coated with Poly-L-ornithine hydrobromide (6 µg/mL in 1xPBS, Sigma-Aldrich, for 1 hour at 37°C, SigmaAldrich) followed by Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (1.2 mg/mL in 1xPBS for 1 hour at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were reduced with 5 mM Tris(2-carboxyethyl) phosphine (Thermo Fischer Scientific) at 90°C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) for 30 min at 20°C in the dark ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... or it was offered in mixture with 5mg/ml 3IY (CAS: 70-78-0, Sigma, Steinheim, Germany; stored at −20°C) or in mixture with 5mg/ml 3IY plus 10mg/ml 3,4-dihydroxy-L-phenylalanine (L-DOPA) ...
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bioRxiv - Immunology 2024Quote: ... Viral transduction was performed by spinfection at 900 g at 25 °C for 3 h with 10 μg/ml polybrene (Sigma-Aldrich), followed by resting for 3 h at 37 °C in 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... Flow-troughs were left to incubate for 17 h at 37 °C before the tryptic digestions were terminated with the addition of trifluoroacetic acid (TFA, Sigma-Aldrich) at a final concentration of 0.6% ...
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bioRxiv - Immunology 2024Quote: ... at 37°C in pre-warmed epithelial cell removal solution (HBSS supplemented with 4% iHS [Sigma] and 5 mM EDTA [Sigma]). The remaining tissues containing lamina propria and muscularis layer were digested for 20 min digestion ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Microbiology 2024Quote: ... virus supernatants (30 ml) were treated for 1 h at 37°C with 2500 U ultrapure benzonase nuclease (Sigma, E8263-25KU) and subtilisin64 (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... Before infection HTLV -1 producing cell lines were treated for 90 min with 20 ug/ml of mitomycin C (Sigma Aldrich) to inhibit replication/proliferation of producer cells ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were separated on 10% PAA gel and incubated overnight at 4 °C with anti-Tubulin (Sigma, #T6074, dilution 1:10,000). HRP-conjugated anti-mouse IgG binding protein (Santa-Cruz ...
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bioRxiv - Molecular Biology 2024Quote: ... All mammalian cell lines were cultured in a humidified 5% CO2 incubator at 37 °C and routinely tested for mycoplasma (Sigma-Aldrich). HEK293F cells were obtained from Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... All mammalian cell lines were cultured in a humidified 5% CO2 incubator at 37 °C and routinely tested for mycoplasma (Sigma-Aldrich). RPMI1640 and DMEM media were supplemented with 100 U ml−1 penicillin and 100 µg ml−1 streptomycin (Gibco ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and 15 mL/L of a 10% methyl-4-hydroxybenzoate solution in ethanol) supplemented with 0.5% erioglaucine dye (brilliant blue R, FD&C Blue No.1, Sigma-Aldrich, #861146) and allowed to feed for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... NMR spectra were recorded on a Bruker Ultrashield 500 MHz Plus system at 25 °C using deuterated solvents obtained from Sigma-Aldrich. MALDI-ToF-MS was performed on a Shimadzu Axima Performance instrument in or positive-reflector mode ...
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bioRxiv - Biophysics 2024Quote: ... The resins were functionalized with hydrazine by shaking the resin at 30 °C for 30min twice in 5% hydrazine monohydrate (Sigma Aldrich) in DMF (VWR) ...
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bioRxiv - Genetics 2024Quote: ... 1.6 mg of total proteins were diluted with HEPES lysis buffer to a final volume of 2.4 mL and incubated overnight with 80 µL of 50% anti-c-Myc agarose beads (Sigma-Aldrich, A7470) on a tube rotator ...
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bioRxiv - Developmental Biology 2024Quote: ... immune cell samples were first heat inactivated at 70°C for 10 mins and then subjected to metabolite analysis using GOD-POD kit (Sigma#GAGO20) and Triglyceride assay kit (Sigma#T2449 ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated for 3.5 hours at 37°C with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 mM ionomycin (both from Sigma-Aldrich) in the presence of protein transport inhibitor cocktail (eBioscience ...
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bioRxiv - Genetics 2024Quote: ... alkylated proteins were pre-digested for 3 hours at RT using 0.2 µg LysC after which the sample was diluted by adding 3 volumes of 50 mM ammoniumbicarbonate prior to overnight digestion with AspN at 37°C in 1mM methylamine (Sigma-Aldrich). Digests were diluted 1:1 with 2% trifluoroacetic acid and stored at -20°C prior to shotgun proteomics analysis.
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bioRxiv - Systems Biology 2024Quote: ... Embryos are collected at developmental stages between 24 and 48 hpf and fixed overnight at 4°C in a 10% Formalin solution (Sigma, HT501128). (8 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 ug protein samples were boiled at 90°C for 5 minutes in LDS sample buffer (Genscript#M00676 or Millipore#MPSB) containing 5% 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2024Quote: ... Fixed cells were then incubated for 30 min at 37°C in blocking buffer [0.5% (w/v) bovine serum albumin (BSA, Sigma Cat. #A9647), 10% (w/v ...
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bioRxiv - Developmental Biology 2024Quote: ... the heads were dehydrated in 70% ethanol and subsequently immersed in a 0.5% carmine solution (C-1022, Sigma, St. Louis, MO) prepared in 45% boiling acetic acid for 5-10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... we probed the primary antibodies using Duolink PLA probes rabbit PLUS and mouse MINUS at 37 °C for 1 h and then used Duolink In Situ Detection Reagents Red (Sigma Aldrich) to detect the proximity between the proteins of interest ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the membranes were retained by vacuum filtration to a Whatman GF/C glass microfiber filter (Sigma-Aldrich, St. Louis, MO, USA) and the free radioligand was discarded ...