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Citations for Millipore Sigma :
4801 - 4850 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Live-cell AIS staining was performed using a mouse anti-neurofascin antibody targeting an extracellular epitope (NeuroMab clone A12/18, #75-172) conjugated with CF647 (Mix-N-Stain CF647 antibody labeling kit, Sigma-Aldrich #MX647S50) following the manufacturer protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 µM of the nonspecific nitric oxide synthase inhibitor Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME [Sigma Aldrich], n=5), or 1.0 mM P188 and 100 µM L-NAME (n=5) ...
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bioRxiv - Neuroscience 2024Quote: ... An antibody reactive for the N-terminal amino acids 66-81 of the amyloid precursor protein (APP) (Clone 22C11: Millipore, Billerica, MA) was applied at 1:40K and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2024Quote: Recombinant active human MMP-9cd (auto-cleavage resistance) 34 with an N-terminal His6x tag was expressed in Rosetta™(DE3) pLysS (Millipore Sigma) cells using a pET-28a(+)-His6x-MMP-9cd vector as previously described 33,35,36 ...
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bioRxiv - Cancer Biology 2024Quote: ... The injected embryos were then incubated at 30°C for 24 - 48 h and allowed to recover in the presence of N-phenylthiourea (Sigma-Aldrich, P7629) to inhibit melanin synthesis ...
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bioRxiv - Plant Biology 2024Quote: ... The membrane was cut across the molecular mass region of the corresponding proteins and separately probed with the following corresponding antibodies: an anti-β-glucuronidase (N-terminal) antibody (G5420, Sigma‒Aldrich) and an anti-tubulin antibody (T5168 ...
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bioRxiv - Physiology 2024Quote: ... Plates were washed with 1 M NaCl for 10 seconds and then stained for 5 hours using 0.2% amido black (Sigma Product N- 3393) in 1 M NaCl ...
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bioRxiv - Cell Biology 2019Quote: The method of multiple inductions of acute endotoxaemia comprises intraperitoneal administration of 8 consecutive injections (every 3 days) of LPS 10 mg/kg (Lipopolysaccharides from Escherichia coli 026:B6, Sigma-Aldrich®, St. Louis, MO, USA), in saline (NaCl 0,9%) ...
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bioRxiv - Neuroscience 2024Quote: Postnatal day 8 mice received an intraperitoneal (IP) injection 0.6 µg/g of lipopolysaccharide (LPS) from Escherichia coli O55:B5 (Sigma-Aldrich, St. Louis MO, Catalog no. L2880) diluted in sterile 0.9% sodium chloride solution (normal saline) ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: For profiling the wildtype and oncogenic KMT2A protein we used two monoclonal antibodies targeting the KMT2A N-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-764) refered to as KMT2A-N1 ...
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bioRxiv - Biochemistry 2021Quote: ... The effect of metabolites on the thermal stability of SdGA was measured using 0.1 mM acarbose (Sigma-Aldrich cat. n° 56180-94-0), 1 mM CaCl2 or 10% (v/v ...
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bioRxiv - Biochemistry 2020Quote: ... Multiple clones were isolated and successful integration of N-terminal 3xFLAG tag was validated by western blotting with anti-FLAG-HRP (Sigma, A8592, 1:10,000).
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bioRxiv - Cancer Biology 2021Quote: ... Purification of N-glycans from the reaction mixture was done on the Supelclean™ ENVI-Carb™ SPE graphitized carbon tubes (Sigma-Aldrich). Prior to purification ...
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bioRxiv - Neuroscience 2020Quote: LC-MS grade water was purchased from Honeywell (Riedel-de Hae n; LOT: I174P) and formic acid was purchased from Sigma-Aldrich (LOT: 179247). LiChromasolv hypergrade Acetonitrile (LOT ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then mounted in 12 µL of mounting media (20 M N-propyl gallate [Sigma-Aldrich] and 0.14 M Tris in glycerol) with a no ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Biophysics 2020Quote: Goat anti-mouse IgG was incubated using a 5 x excess of N-hydroxysuccinimidyl esters – Alexa647 (LifeTech, A20106) and purified after 4 h using spin-desalting columns (40K MWCO) in PBS (Sigma-Aldrich, D8537-500ML) to remove excess dyes ...
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bioRxiv - Biochemistry 2022Quote: Labeling buffers were made from lyophilized aliquots of buffer A and were directly resolubilized in D2O (99.9% atom D, Sigma Aldrich P/N 151882) or after adding 6M Urea-d4 (98% atom D ...
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bioRxiv - Cell Biology 2022Quote: ... and cultured in expansion medium (ADMEM/F12 supplemented with 1% penicillin/streptomycin, HEPES, GlutaMAX, N-acetylcysteine (1 mM; Sigma-Aldrich, Zwijndrecht, the Netherlands) and 1.5% B27 supplement (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were transferred to an 8-well glass-bottom high-wall ibidi chamber and covered with an imaging buffer consisting of 700 mM N-Acetyl-Cysteine (Sigma-Aldrich, A9165-100G) in ddH2O ...
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bioRxiv - Biochemistry 2022Quote: ... and 78.1 µL of a 10 mg/mL (1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl N-succinimidyl carbonate (BCN, 17 eq.) (Sigma Aldrich, Cat. No. 744867-10MG) and stirred at 4°C for 16 h ...
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bioRxiv - Plant Biology 2021Quote: ... was analyzed by extracting the gDNA with either the innuPREP Plant DNA Kit (Analytik Jena; Jena, Germany; T0) or the REDExtract-N-AMP plant Kit (Sigma Aldrich; T1, T2). The transgene was detected by amplifying the Ocs terminator of the hygromycin resistance cassette (Table S3).
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bioRxiv - Physiology 2021Quote: Total RNA was extracted from snap frozen tissue (n=10 liver and quadricep muscle) and cells using Tri-Reagent (Sigma-Aldrich, Dorset, UK) and concentration determined spectrophotometrically at OD260 on a Nanodrop spectrophotometer (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: Methanol-fixed infected cells and a methanol-fixed negative control were incubated overnight at 4 C° with 150 μl of a solution of anti-S IgG or anti-N IgG in PBS + 3% BSA (Sigma-Aldrich, Milan, Italy). For colocalization experiments ...
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Single cell kinetic modeling of redox-based drug metabolism in head and neck squamous cell carcinomabioRxiv - Systems Biology 2022Quote: ... cells were washed three times with PBS and siRNA packaged in lipid nanoparticles using the N-TER Nanoparticle siRNA Transfection System (Sigma-Aldrich, Cat#N2913) was applied to each well at 50 nM in 100 uL of serum-free media for 4 hours ...
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bioRxiv - Microbiology 2022Quote: ... we filtered 500 ml of the collected water through a 0.22 μm Sterivex filter (Millipore Sigma, Burlington, MA; n=4 per time point) using peristaltic pressure ...
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bioRxiv - Bioengineering 2019Quote: ... and 1,2-distearoyl-snglycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-mPEG 2000, Laysan Lipids, Arab, AL) into propylene glycol (PG, Sigma Aldrich, Milwaukee, WI) by heating and sonicating at 80 °C until all the lipids were dissolved ...
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bioRxiv - Immunology 2020Quote: ... To purify devil MHC-I molecules by immunoaffinity purification, devil cells were expanded (1×109 cells/replicate, n=4) using Corning® HYPERFlask® M cell culture vessels (Sigma-Aldrich), harvested and washed in 1X-PBS before being snap frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Cancer Biology 2020Quote: ... released crypts were mixed with 10ml DMEM Basal Media (Advanced DMEM F/12 containing Pen/Strep, Glutamine, 1mM N-Acetylcysteine (Sigma Aldrich A9165-SG)) containing 10 U/ml DNAse I (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... nitrogen (N) and deuterium (D) isotopes were added: (i) light (L: R0K0) isotopes (12C14N on both Arg and Lys; Sigma-Aldrich: A6969, L8662), (ii ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... female mice received subcutaneous injections (s.c.) of corn oil (CO) (25 ml/kg, vehicle control; n = 6) (#C8267-2.5L, Sigma-Aldrich, St Louise, MO, USA) or a low-dose of TCDD (20 ng/kg/d ...
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bioRxiv - Physiology 2021Quote: ... dams mated with CON and PHF males (n = 10) were fasted for 6hrs and subjected to a standard intraperitoneal (i.p.) GTT with glucose (G5767; Sigma-Aldrich; 1.5g/kg, i.p.). In another cohort at term gestation (E18.5) ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Microbiology 2020Quote: The sequences encoding mature VdAve1 and VnAve1 were cloned into pET-15b with an N-terminal His6 tag sequence (Novagen, Madison, WI, USA) (primer sequences ...
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bioRxiv - Neuroscience 2019Quote: ... Pregnant Wistar rats (293.0–428.7 g) therefore received a single intraperitoneal (i.p.) injection of either poly (I:C) (n=8; P9582, potassium salt, Sigma-Aldrich; Gillingham, Dorset, United Kingdom) at a dose of 10 mg/kg or 0.9% non-pyrogenic sterile saline as a control (n=8 ...
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bioRxiv - Physiology 2020Quote: Bone marrow from Winnie and control mice tibiae (n=4-8 mice/group) was collected by flushing with α-modified essential medium ((α-V1EV1, Sigma, Australia). Bone marrow-derived mesenchymal stromal cells (BM-MSCs ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... boric acid and ascorbic acid as well as reagents required for 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) synthesis were obtained from Sigma-Aldrich (Bangalore, India). Acetone was obtained from Fisher Scientific ...
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bioRxiv - Pathology 2021Quote: ... a subset of mice received an intraperitoneal glucose tolerance test (n=26) using 1.5 g/kg body weight D-glucose (Sigma, St Louis, MO, USA). At the completion of the experiment (8 weeks post-transplant) ...
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bioRxiv - Microbiology 2022Quote: ... FISH probes were labelled with 5(6)-carboxyfluorescein-N-hydroxysuccinimide ester (FLUOS) or with the sulfoindocyanine dyes (Cy3) at the 5’ end of the probes (Sigma Aldrich company, Germany). FISH sample preparation was conducted as described earlier (Fernando et al. ...
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bioRxiv - Developmental Biology 2022Quote: Embryos were harvested at E11.5 and N=3 WT and mutant forelimbs (pooled left and right) were dissociated into single cell suspensions using collagenase (Sigma C9697; 50mg/mL) at 37°C for 15 minutes followed by gentle pipette mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... embryonic kidney pairs from n=11 individual mice were cultured ex vivo on transparent Millicell organotypic cell culture inserts (Merck Millipore, Billerica, MA, USA) and maintained in a 37 °C humidified CO2 incubator for 5 days ...
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bioRxiv - Neuroscience 2022Quote: To prepare cell nuclei on the day of library preparation frozen tissue was promptly transferred from -80 °C storage to a chilled 2mL dounce with chilled Nuclei EZ Lysis Buffer (Sigma-Aldrich N 3408). Sample was dounced 25 times with pestle A and 20 times with pestle B ...
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bioRxiv - Neuroscience 2022Quote: The catalytic activity of NAGLU was determined by hydrolysis of the fluorogenic substrate 4-methylumbelliferyl-N-acetyl-α-glucosaminide (EMD Millipore, Burlington, MA) with a final concentration of 0.1 mM substrate in the incubation mixture as described previously50 ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Neuroscience 2023Quote: To label AMPA receptors in the plasma membrane the N-terminal extracellular domain of GluA2 was stained with monoclonal mouse anti-GluA2 (Millipore, # MAB397, 1:500) for 2 hours without prior permeabilization ...
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bioRxiv - Neuroscience 2023Quote: ... Genotypes of the offspring were verified by PCR amplification of genomic ear DNA using the commercially available REDExtract-N-Amp™ Tissue PCR Kit (#R4775; Sigma-Aldrich, Germany) and primers designed to identify the presence or absence of the knock-in allele ...
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bioRxiv - Immunology 2022Quote: ... were dosed 2 mL/Kg intraperitoneally (IP) twice weekly with either olive oil (N=8) or CCl4 (CCl4:olive oil, 1:3, [v:v], Sigma-Aldrich, St Louis, MO) for 4 weeks ...
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bioRxiv - Neuroscience 2024Quote: ... Immunofluorescence was performed according to standard procedures with antibodies against the N-terminus of the laminin α2 chain (rat monoclonal, 4H8-2, Sigma, Saint Louis, USA), the laminin α1 chain (rat monoclonal ...
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bioRxiv - Systems Biology 2024Quote: ... Abs were then reacted with 10x molar excess of dibenzocyclooctyne-S-S-N-hydroxysuccinimidyl (DBCO-S-S-NH) ester (Sigma Aldrich, 761532-1mg) for 2 hours at room temperature in the dark ...