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Citations for Millipore Sigma :
4401 - 4450 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used include anti-α and β-Synuclein (EP1646Y, N-terminal rabbit monoclonal, 1:100 dilution; Millipore, Billerica, MA), anti-pS129 αSyn (81A ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Cell Biology 2020Quote: ... the 1.01 µm diameter carboxylated silica microspheres (SC04000, Bangs Beads) were activated by amine-reactive crosslinker chemistry using NHS (N-hydroxysuccinimide, 130672-5G, Sigma) and functionalized by gelsolin (HPG6-A ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS and 50mM Tris pH 8.0) supplemented with 10 mM freshly prepared N-ethylmaleimide and protease inhibitors (Sigma-Aldrich). Washed GFP-Trap A beads (Chromotek ...
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bioRxiv - Microbiology 2019Quote: ... patient sera were pre-incubated for 10mins with anti-histone single-chain variable fragment (ahscFv; 200 μg/ml, synthesis described previously (18)) or with non-anticoagulant N-acetyl heparin (200 μg/ml; Sigma).
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bioRxiv - Cancer Biology 2019Quote: PyMT [FVB/N-Tg(MMTV-PyVT)634Mul/J] transgenic animals harboring breast tumors were treated with 4-hydroxytamoxifen (Sigma-Aldrich) (1.2 mg/kg/daily ...
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bioRxiv - Biochemistry 2020Quote: ... Fl-SNED1 and the N-terminal fragment were purified by affinity chromatography on an anti-FLAG resin (Sigma-Aldrich, A2220) as previously described (58 ...
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bioRxiv - Plant Biology 2021Quote: ... The released N-glycans were purified on graphitized carbon SPE columns (Supelclean™ ENVI-Carb™, Sigma-Aldrich, Poznań, Poland) and labeled with a fluorescent tag (anthranilic acid ...
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bioRxiv - Plant Biology 2020Quote: ... the pellet was resuspended in minimal volume of 50 mM MOPS (pH 7.5) before being digested with Asp-N endoproteinase (Sigma-Aldrich) at 1:50 (enzyme:acyl-ACP ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured in MDCK medium and after inoculation in infection medium containing N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (3µg/mL; Sigma, T1426).
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Neuroscience 2019Quote: ... injection of saline (10 ml/kg body weight) or clozapine N-oxide (CNO, 10 mg/kg, 10 ml/kg, Sigma). Drugs (saline or CNO ...
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bioRxiv - Neuroscience 2020Quote: ... two brains were pooled (n=6) and homogenized in 300 µL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich). Tissue preparation and biochemical analysis followed protocols described in (Sachett et al. ...
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bioRxiv - Neuroscience 2021Quote: All primers were provided from Thermo Fisher and the REDExtract-N-Amp PCR Reaction Mix™ reagent used for the polymerase reaction was provided from Sigma-Aldrich® ...
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bioRxiv - Biochemistry 2020Quote: ... the reactions were stopped with EDTA buffer and the samples incubated with 0.1U/mL of chondroitinase ABC (AMS Biotechnology, Abingdon, UK) and keratanase (endo-beta galactosidase, Cat. n.: G6920, Sigma Aldrich) in deglycosylation buffer (50mM sodium acetate ...
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bioRxiv - Bioengineering 2020Quote: ... Lyophilized samples were then digested in papain solution (100mM Na2PO4, 5mM N-Acetyl-L-Cysteine, 5mM EDTA, 3.875 U/mL Papain (Sigma-Aldrich)) at 65°C overnight ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction was initiated by the addition of 1 mM N-α-benzoyl-L-arginine-p-nitroanilide substrate (Sigma-Aldrich) and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
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bioRxiv - Neuroscience 2021Quote: ... three brains were pooled (n=6) and homogenized in 450 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Cancer Biology 2021Quote: 105 HCT116 cells as well as HCT116 cells with acquired oxaliplatin resistance and HCT116 cells with BOLD-100/KP1339 resistance were seeded (N = 4 for each respectively) into 12-well plate (StarLab) with 2 mL McCoy’s 5A medium (Sigma-Aldrich) containing 10% FCS (BioWest ...
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bioRxiv - Cell Biology 2021Quote: ... Collected supernatant and lysed pellet were separately incubated with 3.4 mg/mL p-nitrophenyl-N-acetyl-β-D-glucosaminide (NAG, Sigma) in citrate buffer (pH 4.5 ...
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bioRxiv - Cell Biology 2021Quote: Liver nuclei (n=4) were prepared from frozen tissue specimens by crushing and dounce homogenising the tissue in 1 ml EZbuffer (SIGMA) (20 strokes with loose and a tight pestle ...
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bioRxiv - Biochemistry 2021Quote: ... Cysteine Biotinylation was carried out using Poly(ethylene glycol) [N-(2-maleimidoethyl)carbamoyl]methyl ether 2-(biotinylamino)ethane (Sigma 757748) (Biotin-maleamide) ...
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bioRxiv - Biochemistry 2020Quote: ... Specifically the dried extracts were dissolved in 0.2-mL acetonitrile and silylation was performed by the addition of 0.05-mL of N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) (Sigma-Aldrich), and incubated at 65°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was centrifuged and the resulting supernatant was run through a column filled with N-NTA His-band resin (Millipore). Bound protein was eluted and then dialyzed for overnight in PBS at 4□ ...
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bioRxiv - Plant Biology 2020Quote: ... was cloned using cDNA from Arabidopsis seedlings and inserted in frame with an N-terminal His6-tag between the BamHI and EcoRI sites of the pETDuet-1 vector (Novagen). Protein expression was performed in E ...
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bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
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bioRxiv - Cell Biology 2020Quote: ... was added at equal volume and supplemented with 10 μL anti-fade solution (9 parts glycerol, 1 part 10X PBS, 0.1 part 20%(w/v) n-propyl gallate (Sigma P3130) in dimethyl sulfoxide) ...
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bioRxiv - Neuroscience 2019Quote: ... rats were injected (1 mL/kg; i.p.) with either the cannabinoid agonist CP-55,940 (n=36; C112-10MG; Sigma-Aldrich, Inc.) or a control vehicle (n=11 ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants were immediately used for measurement of proteasome peptidase activity by cleavage of the fluorogenic peptide N-Suc-LLVY-MCA (Sigma), enzymatic activity were acquired for 2h ...
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bioRxiv - Microbiology 2021Quote: ... 5 µg of purified MurA protein were mixed with 10 mM uridine 5′-diphospho-N-acetylglucosamine (UDP-GlcNAc, Sigma-Aldrich) in a reaction buffer containing 100 mM Tris-HCl pH8.0 and 150 mM NaCl (final volume ...
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bioRxiv - Plant Biology 2021Quote: ... Free hydroxyl FAs were derivatized at 110°C for 30 min in 100 µL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA, Sigma) and pyridine (50:50 ...
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bioRxiv - Neuroscience 2021Quote: ... four brains were pooled (n=6) and homogenized in 600 μL of phosphate-buffered saline (PBS, pH 7.4, Sigma-Aldrich) and centrifuged at 10,000 g at 4 °C in a cooling centrifuge ...
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bioRxiv - Cell Biology 2021Quote: ... cycloheximide (C4859) and the tankyrase inhibitor IWR-1 (I0161) and the deubiquitinating enzyme inhibitor N-Ethylmaleimide (NEM) (E3876) were from Sigma. The tankyrase inhibitor Az6102 (S7767 ...
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bioRxiv - Cell Biology 2020Quote: ... mADD1 has 6xHis tag at its N terminus followed by TEV protease recognition site and the plasmid was transformed into Rosetta (DE3) pLysS cells (Novagen). Target protein was expressed in cultures grown in autoinduction media at 18°C overnight 48 ...
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bioRxiv - Cell Biology 2021Quote: ... 50 mg/mL EDC and NHS solutions were prepared in 25 mM cold 2-(N-morpholino) ethanesulfonic acid (MES, cat. num. M3671-50G, Sigma), at pH5 ...
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bioRxiv - Biophysics 2022Quote: ... in this work referred to as Pif1) fused at its N-terminus to a 6-histidine tag was cloned into vector pET28 (Novagen) and transformed into E ...
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bioRxiv - Biochemistry 2022Quote: ... the protein sequence fused to an N-terminal GB1 solubility tag and a 6x TEV-cleavable His-tag was cloned into pET24b (Novagen). Plasmids were transformed into E ...
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bioRxiv - Pathology 2022Quote: ... The resulting dried extracts were derivatized using methyoxyamine hydrochloride (MOX) and N,O-Bis(trimethylsilyl)trifluoroacetamide (TMS) [both purchased from Sigma] ...
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bioRxiv - Microbiology 2022Quote: ... BHI was supplemented with BC (n-alkyl dimethyl benzyl ammonium chloride, Sigma-Aldrich, St. Louis, MO, CAS: 63449-41-2) to achieve appropriate BC target concentrations ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The likely elution volume of molecules of interest was established by elution of 20 nmols UDP-MurNAc-DAP-pentapeptide and 20 nmols UDP-N-acetyl-glucosamine (Sigma) standards.
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bioRxiv - Developmental Biology 2022Quote: ... a small population (n≈10) of 3rd instar larvae were embedded in a previously heated 5% m/v carboxymethylcellulose (Sigma) solution ...
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bioRxiv - Microbiology 2022Quote: Sialidase activity was fluorometrically monitored using the substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4MU-Neu5Ac; Sigma-Aldrich). Reactions were performed in a final volume of 50 μL ...
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bioRxiv - Molecular Biology 2022Quote: ... N-acetyl-L-cysteine (NAC; #S0077) and methylthiazolyldiphenyl tetrazolium bromide (MTT; #2128) were products from Sigma-Aldrich (St Louis, MO). Mitochondria-targeted antioxidant (MitoQ ...
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bioRxiv - Biophysics 2022Quote: Myristoylated c-Src constructs were obtained by co-expression of the N-myristoyl transferase (NMT) enzyme along the c-Src USH3-His6Tag construct in a pETDuet-1 (Novagen) plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to the culture media for 8 hours before preparing protein lysates with the above lysis buffer supplemented with N-Ethylmaleimide (25mM, Sigma). Lysates were pre-cleared using Protein G sepharose beads (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: Live TTX-treated WT and Fmr1R138Q hippocampal neurons (14-15 DIV) were incubated with a mouse monoclonal anti-N-terminal GluA1 antibody (1:100; Merk-Millipore) for 10 min at RT ...