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Citations for Millipore Sigma :
4551 - 4600 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Human neuroblastoma cell line SK-N-SH (ATCC HTB-11) was cultured in Dulbecco’s Modified Eagle’s Medium with 1g/L glucose (Sigma-Aldrich, FIN) supplied with 10 % FBS ...
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bioRxiv - Cell Biology 2023Quote: ... cells were pelleted after 6 h or 12 h and resuspended in SD-N containing 30 mg/l L-lysine-13C6,15N2 hydrochloride (Lys8, Sigma, 608041) and incubated for indicated time ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... N’-tetramethylethane-1,2-diamine (TEMED) and 1% (v/v) of solution of 10% (w/v) ammonium persulfate (APS) (Sigma-Aldrich) was added to the mixture and vortexed to initiate the polymerisation ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Cell Biology 2024Quote: ... Two µl of colorless hemolymph was aspirated from each pile and separately transferred to 96-well plates (Thermo-Scientific) containing 0.1% N-Phenylthiourea (Sigma-Aldrich) in 50Lµl PBS ...
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bioRxiv - Microbiology 2024Quote: ... The resultant protein with an N-terminal His6-tag was concentrated using a 10 kDa Amicon Ultra-15 concentrator (Millipore). Protein concentrations were determined from the absorbance at 280 nm using a molar absorptivity constant (ε280 = 22,920 M-1 cm-1 ...
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bioRxiv - Bioengineering 2024Quote: ... The mixture was neutralized with 1 N acetic acid until gas evolution ceased and filtered through Celite 545 (EMD Millipore). The solution was concentrated by rotary evaporation at 50 °C and precipitated in cold diethyl ether (Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Infant (n=1) and adult (n=1) hippocampal sections (10μm) were fixed in 10% neutral buffered formalin (catalog no. HT501128, Sigma-Aldrich) for 30 minutes at RT ...
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bioRxiv - Biochemistry 2024Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography ...
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bioRxiv - Biochemistry 2024Quote: N-Lactoyl Phenylalanine (catalogue # 318943) was purchased from Novo Pro Labs and 1-Carboxyethylphenylalanine (ENAH95E73EBB) was purchased from Sigma Aldrich.
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bioRxiv - Immunology 2024Quote: ... followed by activation with 50 mM 3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride (EDC)/100 mM N-Hydroxysuccinimide (NHS) (Sigma-Aldrich) for 12 min at 10 ml/min ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... were identified and quantified by gas liquid chromatography (Perichrom) as their alditol acetates obtained after hydrolysis (4 N trifluoroacetic acid (TFA, Sigma), 100 °C ...
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bioRxiv - Immunology 2024Quote: HAP1 target cell lines were plated one day prior to the coculture with γδ T cells at a density of 15,000 cells/well in a flat-bottom 96-well plate and treated O/N or not with 10 µM pamidronate (PAM, Sigma) in complete IMDM (includes 10% FCS ...
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bioRxiv - Bioengineering 2024Quote: ... A 1 wt% alginate solution was prepared in 0.1 M 2-(N-morpholino)ethanesulfonic acid buffer (MES, Sigma Aldrich, M8250). Sulfonated N-hydroxysuccinimide (NHS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA was extracted from the euthanized embryos using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... In the reciprocal experiment 1 mg of N-terminally his-tagged Saci_1104 was loaded onto a Nickel-NTA (Sigma, Germany) column (column volume of 250 µl) ...
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bioRxiv - Cell Biology 2024Quote: Tubulin was purified from porcine brain using established protocols as described previously [39] Polarity-marked microtubules were prepared by preferably extending the plus-ends of long GMPCPP seeds in the presence of N-ethylmaleimide (Sigma) modified tubulin (NEM-tubulin) ...
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bioRxiv - Biochemistry 2024Quote: ... coli including a polyhistidine tag and thrombin cleavage site at its N-terminus was amplified via PCR and subcloned into pET-30b (Novagen) between the NdeI/ BamHI sites (Vermot et al. ...
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bioRxiv - Cell Biology 2021Quote: ... nitrocellulose membranes were incubated with primary antibodies against α-E catenin (1: 1000; Cell Signalling Technologies, catalogue number # 240) and α-tubulin (1: 20 000; Sigma–Aldrich, catalogue number T6074) at 4°C ...
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bioRxiv - Genetics 2021Quote: ... coli OP50 supplemented with or without vitamin B12 (methylcobalamin or MeCbl, Sigma, Cat #13422-55-4; adenosylcobalamin or AdoCbl, Sigma, Cat #13870-90-1) and propionic acid (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... glycerol stock containing the uropathogenic Escherichia coli strain UTI89 was used to inoculate a Miller Luria Broth (LB) agar plate (Sigma-Aldrich, St. Louis, MO). The plate was incubated for 24 hours at 37°C ...
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bioRxiv - Immunology 2021Quote: ... mice were weighed and immediately administered an intraperitoneal injection of lipopolysaccharide (7.5 × 105 EU/kg) from Escherichia coli O111:B4 (Millipore Sigma, Temecula, CA; Cat# L4391). Two hours post-LPS injection ...
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bioRxiv - Immunology 2020Quote: ... Classically activated M1 phenotype was achieved by exposure to 100 ng/mL LPS from Escherichia coli O111:B4 (Sigma Aldrich; St. Louis, MO, USA) and 20 ng/mL recombinant human IFN-γ (PeproTech ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid that contains the kinase domain of human HASPIN was transformed into Escherichia coli strain Rosetta™ 2(DE3) pLysS (Novagen, Madison, WI, USA). The transformed cells were grown at 37□ until OD600 reached 0.8 ...
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bioRxiv - Immunology 2022Quote: ... 2-4×104 bone marrow-derived DCs (BMDCs) were re-plated in 96-well round-bottom plates and stimulated with 1.0 μg/ml LPS (Sigma L2762: Escherichia coli 26:B6) and different concentrations of peptides for 24 hours ...
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bioRxiv - Immunology 2023Quote: Propionate SCFA (catalog number P1880) and lipopolysaccharide (LPS, catalog number L4391, from Escherichia coli O111:B4) were purchased from Sigma-Aldrich (St. Louis, MO). Seahorse XF calibrant solution (part number 100840-000) ...
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bioRxiv - Immunology 2023Quote: ... Lipid A purified from Salmonella minnesota (Re-595) and lipopolysaccharide (LPS) from Escherichia coli (O55:B5) were purchased from Sigma-Aldrich (Merck, Darmstadt, Germany). Pam3CSK4 ...
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bioRxiv - Immunology 2023Quote: Human FM explants were treated with either a no treatment (NT) media control or 100ng/ml LPS isolated from Escherichia coli O111:B4 (Sigma-Aldrich, St Louis, MI) for 48 hours ...
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bioRxiv - Plant Biology 2020Quote: ... 150 uM stock trans-zeatin in .01 N KOH was added to autoclaved media to create 150 nM working concentration (Sigma, Z0876). For the NPA experiment ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then collected and re-plated at 500 cells/well with either 5 μM 4-nitroquinolone N-oxide (4NQO) (Sigma, N8141) or a dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2021Quote: ... cantilever B with nominal spring constant of 0.02 N/m) (Bruker, USA) with a 25 µm diameter polystyrene microsphere (Sigma Aldrich, USA) glued to the free end were used in all AFM measurements ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C in a wet chamber with the following antibodies: anti-P2X7 (cat. n. P8232, Sigma-Aldrich) and anti-Tom 20 (cat n ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Developmental Biology 2021Quote: ... Rats were screened for the presence of mutations by PCR using tail-tip DNA samples (RED extract-N-Amp Tissue PCR Kit, Millipore-Sigma) as described previously (1 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Steidle and Dettner, 1993) in 70 μl hexane (company, GC/MS analytical grade) containing 150 ng/μl n-octadecane (Sigma Aldrich) as an internal standard ...
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bioRxiv - Molecular Biology 2020Quote: ... and mice exposed to 5 days of photo-oxidative damage (PD) (n=6) were lysed in CellLyticTM Cell Lysis Buffer (Sigma-Aldrich) supplemented with a protease inhibitor cocktail (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 5% lysed horse blood (defibrinated 50% stock, Hatunalab AB Cat. N° 139) and 20 mg/mL β-NAD (Sigma, Lot# SLCD5502). Prior to use the 50% horse blood stock was freeze thawed five times and clarified via centrifugation twice for 30 minutes at 18,000 rpm at 4 °C and then filtrated using 0.2 μm membrane filter ...
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bioRxiv - Genomics 2020Quote: ... Subsequently sections were incubated for 1h at RT in PBS blocking buffer containing 5% horse serum (Sigma Aldrich, Cat. N: H0146), 0.3% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Immunology 2020Quote: ... The bioswitch labeling procedure was performed according to the protocols described in (11, 45) with modifications using N-ethylmaleimide (NEM, Sigma Aldrich) to alkylate the reduced thiols ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we treated females in the Pregnancy GCs (n = 44 total litters from 43 unique females) and Lactation GCs (n = 18 litters from 17 unique females) treatment groups with exogenous cortisol (hydrocortisone, Sigma H4001) dissolved in peanut butter and wheat germ mixture (8 g of peanut butter ...
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bioRxiv - Microbiology 2019Quote: ... cells were transferred (2% inoculation) into 50 ml of T-R minimal medium supplemented with 0.5% (w/v) N-acetylglucosamine (Sigma-Aldrich #G4875) as a carbon source and incubated ~12 hr at room temperature and 150 rpm ...
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bioRxiv - Developmental Biology 2019Quote: ... Retinal explants were grown as for DRGs but without NGF and with medium supplemented with N-2 (Sigma-Aldrich, 100x concentrate) and bovine pituitary extract (200μg/ml ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Peaks were tentatively identified by their mass spectra and retention time index based on a C21-C40 n-alkane standard solution (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... whole brains of females (n = 6) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Molecular Biology 2019Quote: ... tendon fascicles (n=6, independent samples from different mice) were stained with 2’,7’-Dichlorofluorescin diacetate (DCFH-DA, D6883, Sigma Aldrich). Control fascicles and cultured fascicles were incubated for 30 minutes with 50μM DCFH-DA in PBS at either 29°C ...
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bioRxiv - Microbiology 2021Quote: ... a blocking step of incubation with sheep serum for 1 hour was followed by immunostaining for N using a mouse monoclonal antibody (αN009 48) and detected using a sheep anti-mouse FITC conjugate (Sigma, UK). Phalloidin Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Bioengineering 2021Quote: ... The MCF-7 and the SK-N-SH cells were cultured in RPMI 1640 and in Minimum Essential Medium Eagle (MEM) from Sigma Aldrich, respectively ...