Labshake search
Citations for Millipore Sigma :
4601 - 4650 of 4935 citations for SARS Coronavirus Spike Glycoprotein S1 Mosaic N Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: We fused FtsE at its N-terminus to a 6xhis-followed by a TEV protease cleavage site in vector pETDuet™-1 (Novagen) that allows tunable expression of the fusion protein from a T7 promoter induced by isopropyl-β-D-1-thiogalactopyranoside (IPTG) ...
-
bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on two connected in series 1 ml Heparin-Sepharose columns (Cytiva ...
-
bioRxiv - Biochemistry 2021Quote: ... N-containing fractions were concentrated on the Amicon Ultra-4 Centrifugal Filter Unit (Merck/Millipore; molecular weight cut-off 30.0 kDa) and loaded on a Superdex 200 10/300 GL size exclusion column (GE Healthcare Life Sciences ...
-
bioRxiv - Neuroscience 2020Quote: ... myotube compartments were treated with 19 μM of the AChR competitive antagonist tubocurarine hydrochloride pentahydrate (DTC) (Sigma, Cat N° T2379-100G) 10 min before analysis ...
-
bioRxiv - Neuroscience 2021Quote: ... Specimens were incubated overnight at 4°C in 1:5000 primary antibody (monoclonal anti-alpha-tubulin; Sigma Aldrich, cat n. T5168) diluted in BSA 1% + sheep serum 2% in PBT ...
-
bioRxiv - Biophysics 2020Quote: ... The coating was done in the following three step procedure: I) 1mL of the resulting solution was added to 11.5 mg NHS (N-Hydroxysuccinimide, Sigma-Aldrich, Germany) and vortexed ...
-
bioRxiv - Neuroscience 2020Quote: ... N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 0.3 µg/0.5 µL/hemisphere; n = 9; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
-
bioRxiv - Neuroscience 2020Quote: ... Dimethyl sulfoxide (DMSO) and clozapine-N-oxide (CNO; agonist at hM3Dq receptors) were purchased from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of CNO (0.1 mg/ml in saline solution containing 0.5% DMSO was prepared and stored in aliquots ...
-
bioRxiv - Microbiology 2021Quote: ... which were then derivatized by adding 100 μl 90% N,O-bis(trimethylsilyl)-trifluoroacetamide-10% tetramethylsilane (TMS) (Sigma, St. Louis, MO) and 200 μl anhydrous pyridine (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... EPR spin-trapping studies were performed using the spin-trapper 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) (Sigma, St. Louis, MO) at 10 mM ...
-
bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 15 min and inspected for sample quality control following Hoechst staining in imaging buffer (IB: 700 mM N-acetyl-cysteine (Sigma, A7250) in ddH2O ...
-
bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
-
bioRxiv - Cell Biology 2021Quote: Labeling reagents XTT (sodium 3’-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy6-nitro) benzene sulfonic acid hydrate) and PMS (N-methyl dibenzopyrazine methyl sulfate) were purchased from Sigma-Aldrich. Assay was performed according to protocol in Cell proliferation kit II (XTT ...
-
bioRxiv - Cancer Biology 2022Quote: ... The solution was neutralized using sodium acetate pH 7.0 and DNA sample was transferred to Whatman Hybond N+ Blotting Membrane (Millipore Sigma, Z761079). Nucleic acids were cross-linked by to the membrane by baking at 60 °C for 30 min followed by UV exposure ...
-
bioRxiv - Cell Biology 2022Quote: ... and incubated o/n at 4°C with the following primary antibodies at a dilution of 1/1000: anti-LC3 (Sigma, L7543), anti-Atg5-12 (Abnova ...
-
bioRxiv - Plant Biology 2022Quote: Proteasome activity was measured using the fluorogenic substrate N-Succinyl-Leu-Leu-Val-Tyr-7-Amido-4-Methylcoumarin (Millipore Sigma; S6510) as described Üstün et al ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were washed three times in DPBS and incubated in DMEM supplemented with 100 ng/mL anhydrotetracycline and 10 mM N-acetyl cysteine (NAC, Sigma-Aldrich) when indicated ...
-
bioRxiv - Cell Biology 2022Quote: ... It was inserted with N and C-terminal tags (His6 and StrepII, respectively) in the baculovirus transfer vector pIEX/Bac3 (Merck Novagen #717263).
-
bioRxiv - Immunology 2022Quote: ... and mesenteric) (n=3) were pooled in digestion medium consisting of RPMI 1640 with 2% fetal bovine serum (FBS) (Sigma-Aldrich), deoxyribonuclease (DNase ...
-
bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
-
bioRxiv - Microbiology 2022Quote: ... 3-(N-Morpholino)propanesulfonic acid (MOPS) or 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) buffer (all purchased from Sigma-Aldrich, USA). Macrocolonies were excised and resuspended in 2 mL of sterile 1X PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cysteine containing phosphopeptide was conjugated to carrier protein Limulus hemocyanin using hetero-bifunctional crosslinker m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester (Sigma# 803227). This conjugate was used to immunize New Zealand white rabbits (Charles River Laboratories) ...
-
bioRxiv - Bioengineering 2022Quote: ... The top flow channel was perfused with hECSR and the bottom chamber with N-Formylmethionyl-leucyl-phenylalanine (fMLP, 10 nM, Sigma Aldrich), a potent neutrophil chemoattractant ...
-
bioRxiv - Cancer Biology 2022Quote: ... Actin (#A1978) and DCLK1 (targeted to the N-terminus, #62257) (#SAB4200186) were purchased from Sigma Aldrich (Sigma-Aldrich, St. Louis, MO). Bax (#5023) ...
-
bioRxiv - Immunology 2021Quote: ... Monomers were loaded with 0.2 mg/ml peptide at 37°C for 72 h in the presence of 0.2 mg/ml n-Dodecyl-β-maltoside and 1 mM Pefabloc (Sigma-Aldrich). Peptide-loaded monomers were conjugated into tetramers using R-PE streptavidin (Invitrogen) ...
-
bioRxiv - Plant Biology 2021Quote: ... 5-DAG seedlings were treated with 0.8 M mannitol [32] with 0.1% (w/v) Calcofluor white (Sigma-Aldrich, cat. n. 18909) for 30 min ...
-
bioRxiv - Immunology 2021Quote: ... CD4+ T cells from iCD4-PRMT5fl/fl and iCD4-PRMT5Δ/Δ mice (n = 3 pooled mice/sample and n = 3 samples/group) were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3’-phosphoadenosine-5’-phosphosulfate (PAPS), and [1,2,6,7-3H(N)]dehydroepiandrosterone ([3H]DHEA, 60 Ci/ mmol) were purchased from Sigma-Aldrich (St. Louis,MO). SDS–polyacrylamide gel electrophoresis reagents were obtained from Bio-Rad (Hercules ...
-
bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
-
bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
-
bioRxiv - Neuroscience 2021Quote: ... catalog #96121229) were cultured as described (N. J. Ortner et al., 2020) in Dulbecco’s modified Eagle’s medium (DMEM; Sigma-Aldrich, catalog #D6546) that was supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Bioengineering 2021Quote: U87 glioblastoma (ATCC HTB-14) and SK-N-BE(2) neuroblastoma (ATCC CRL-2271) were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (Sigma-Aldrich, Australia) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2021Quote: Desiccated tissue sections mounted on ITO glass slides were sprayed using an HTX TM-Sprayer (HTX Technologies, LLC) with 10 mg/ml N-(1-Naphthyl) ethylenediamine dihydrochloride (NEDC, Sigma #222488) dissolved in 70%:30% methanol:water ...
-
bioRxiv - Immunology 2021Quote: ... we extracted genomic DNA from mouse tails and performed PCR with REDExtract-N-Amp™ Tissue PCR Kit following the manufacturer’s instructions (Sigma-Aldrich) and analyzed samples on agarose gel ...
-
bioRxiv - Cell Biology 2022Quote: ... Pigmentation was prevented during experiments by treating the embryos with 0.002% N-phenylthiourea (PTU) (Sigma-Aldrich, St. Louis, US-MO, #P7629) from 1dpf onwards ...
-
bioRxiv - Developmental Biology 2022Quote: Mice were genotyped by PCR using ear biopsies collected within 4 weeks of birth and genomic DNA was extracted using Extract-N-Amp tissue prep kit (Sigma-Aldrich). Embryos were genotyped using either immune-reactivity to antibody raised against either STAT3 pY705 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Bioluminescent spectra was then acquired after VK3 and NAC (N-acetylcysteine) treatment or auranofin and buthionine sulfoximine (both Sigma, Burlington, MA) treatment or BBI-608 (Boston Biomedical ...
-
bioRxiv - Plant Biology 2019Quote: ... Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen, Wisconsin, USA) and transformed into E ...
-
bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
-
bioRxiv - Plant Biology 2019Quote: ... 5 mM EDTA, 150 mM NaCl, 2.5% SDS, 10 mM N-ethylmaleimide, 5 μl.ml−1 protease inhibitor [P9599, Sigma-Aldrich], 100mg PVPP). Samples were mixed gently for 10 minutes at room temperature and centrifuged at 16,000 xg for 1 minute ...
-
bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
-
bioRxiv - Biophysics 2019Quote: The tetra-p-tosylate salt of meso-tetrakis(4-N-methyl-pyridyl)porphine (TMPyP4+, Fig. 1) and potassium salt of poly(rA) from Sigma-Aldrich Chemical Co ...
-
bioRxiv - Biochemistry 2019Quote: ... trimethylsilyl-N-methyl trifluoroacetamide (MSTFA) and N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA) were obtained from CS-Chromatographie Service and pyridine from Sigma-Aldrich at >99.98% purity ...
-
bioRxiv - Genomics 2019Quote: ... The dried extract was then resuspended in 5 μl of a hexane solution containing 7.5 ng/μl of n-dodecane (EMD Millipore Corp.) as an internal standard ...
-
bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
-
bioRxiv - Zoology 2020Quote: ... DNA was extracted from individual cysts or juveniles using the “Sigma Extract-N-Amp Kit (XNAT2)” kit (Sigma, St. Louis, MO) according to the protocol reported by Ma et al ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... directed against the N-terminal half of mouse AHR and the polyclonal goat anti-rabbit secondary antibody cross-linked to alkaline phosphatase (Sigma-Aldrich). The blot was developed using the AP Conjugate Substrate Kit (Bio-Rad).
-
bioRxiv - Biophysics 2020Quote: ... 100 μL aliquots of 50 μM K311Dha in in 20 mM NaPi (pH 8) was reacted with 1000 molar equivalents of N-acetylcysteamine (Sigma Aldrich) for 12 hours at 37 °C and shaking at 500 rpm ...
-
bioRxiv - Plant Biology 2020Quote: ... 0.1 μg/μl) were isolated and solubilized on ice for 5min using 1% β-DDM (n-Dodecyl β-D-maltoside, Sigma) in a buffer containing 0.7 M Sucrose ...