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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were affinity purified by anti-Flag M2 affinity resin (Sigma-Aldrich), which followed digestion of affinity tag and deglycosilation by TEV protease and MBP-fusion endoglycosidase (New England Biolabs) ...
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bioRxiv - Plant Biology 2019Quote: ... and the separated proteins were transferred to PVDF membrane (Millipore, Billerica, MA), stained with Ponceau S for labeling of total protein ...
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bioRxiv - Molecular Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h.
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bioRxiv - Plant Biology 2020Quote: ... and proteins were detected after blotting with anti-FLAG M2 antibody (SIGMA) (1:10,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by ECL (Enhanced chemiluminescence; Merck Millipore, Cat. No. WBKLS0500). The signal was detected using Vilber FUSION-SL system ...
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bioRxiv - Cell Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h and checked by Western blot analysis.
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bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... clarification and benzonase treatment (250 u/1 mg protein, SIGMA E1014-5KU) extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... complexed with 6 mg pre-swollen protein A Sepharose beads (Sigma-Aldrich) during a 2-h incubation at 4°C ...
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bioRxiv - Genetics 2020Quote: ... and proteins were eluted using 100 µg/mL 3x FLAG peptide (Sigma). Eluates were analysed by western blotting with anti-FLAG antibodies (1:5000 ...
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bioRxiv - Cell Biology 2021Quote: ... g) were pre-cleared with protein A-immobilized PureProteome magnetic beads (Millipore) and incubated overnight (4°C ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... 2000 response units (RU) of Protein A from Staphylococcus aureus (Sigma Aldrich) were immobilized on all channels of a CM5 sensor chip ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... 2000 response units (RU) of Protein A from Staphylococcus aureus (Sigma Aldrich) were immobilized on all channels of a CM5 sensor chip ...
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bioRxiv - Neuroscience 2020Quote: ... before the protein expression was revealed by chemiluminescence using Immobilon Western (Millipore) and the signal detected using a Chemidoc MP system (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
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bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cell culture by RIPA lysis buffer (Sigma Aldrich) with protease and phosphatase inhibitor cocktail and sample reducing agent (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-antibody binding was visualized using Immobilon Forte Western HRP Substrate (Millipore) on the LAS-3000 imaging system (FUJIFILM ...
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bioRxiv - Bioengineering 2021Quote: ... filtered through a Millex-HV 0.45-μm low protein-binding membrane (Millipore), and aliquoted ...
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bioRxiv - Bioengineering 2021Quote: ... Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich) in phosphate buffer.
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bioRxiv - Neuroscience 2021Quote: ... Protein was extracted from transfected meningeal cells using RIPA buffer (R0278, Sigma) and run on Western blot at 20ug per well ...
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bioRxiv - Bioengineering 2020Quote: ... total protein was collected from cell lysate in RIPA buffer (Sigma, R0278) containing 1% phosphatase inhibitor cocktail 3 (Sigma ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
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bioRxiv - Biochemistry 2020Quote: ... The partially purified protein was concentrated (10 kDa cut-off, EMD Millipore) and exchanged into 100 mM potassium phosphate buffer (pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was loaded onto a 0.45 μm nitrocellulose filter (HA Millipore), and the filters were washed with 3 ml 30 mM imidazole (pH 7.1) ...
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bioRxiv - Biophysics 2021Quote: Routine chemicals used for protein expression and purifications were purchased from Sigma, Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Supernatant was recovered and proteins were quantified with Bradford assay (Sigma Aldrich) and samples were stored at -80°C until further used ...
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bioRxiv - Physiology 2020Quote: ... Proteins were transferred to an Immunobilon-FL Transfer Membrane (Millipore, Bedford, MA) at 110 volts for 50 min in ice cold transfer buffer ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Transferred protein quantities were detected with Ponseau S solution (Sigma-Aldrich, USA). Transferred proteins were blocked with 1% BSA (KPL Detector™ Block ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and protein was eluted with 100 µg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression and purification and enzyme assays were obtained from Sigma-Aldrich, TCI chemicals ...
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bioRxiv - Biochemistry 2021Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37°C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2020Quote: ... Purified protein was incubated on ice with dried cardiolipin (Sigma-Aldrich, USA) to a final lipid concentration of 0.1 mg/mL ...
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bioRxiv - Biochemistry 2020Quote: Protein Kinase A Catalytic Subunit from bovine heart (PKA, EC 2.7.11.11, Sigma) was reconstituted in bi-distilled water containing 6 mg/ml DTT at a concentration of 50 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized protein was then loaded onto 2 mL M2 affinity matrix (Sigma) previously equilibrated with wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... The resolved proteins were then electrically transferred to a PVDF membrane (Millipore). Immunoblotting was probed with indicated antibodies ...
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bioRxiv - Bioengineering 2021Quote: ... the protein bands were developed using an enhanced chemiluminescent detection kit (Millipore). The optical bands were visualized in a Fuji Las-3000 dark box (FujiFilm) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were preparared using RIPA lysis buffer (Sigma, ST. Louis, USA) supplemented with protease inhibitor cocktail (Complete ...
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bioRxiv - Cell Biology 2021Quote: ... The concentration of the isolated proteins was determined using Bradford reagent (Sigma). 30-50 micrograms of the protein were separated on a 15% polyacrylamide gel and electrophoretically transferred to PVDF membranes (Millipore) ...
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bioRxiv - Plant Biology 2020Quote: ... the proteins were immobilized on to a reinforced nitrocellulose membrane (Millipore Sigma) with 1× NuPage transfer buffer (Thermo Fisher ...
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bioRxiv - Systems Biology 2020Quote: ... Protein G sepharose was used for anti-SOCS1 (Millipore, 04-002, RRID:AB_612104) IP ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated using a Quantipro BCA assay kit (Sigma Alrich). Protein lysates (50 μg ...
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bioRxiv - Neuroscience 2021Quote: Proteins from spinal cord tissue were extracted with RIPA buffer (Sigma Aldrich) containing a cocktail of protease inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total protein was added to 7.8 nmol nitrocefin (Sigma) and absorbance at 495 nm measured kinetically for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Protein content of the cell lysates was determined using BSA assay (Sigma).
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bioRxiv - Plant Biology 2021Quote: Proteins were purified using HisSelect Sepharose beads (Sigma-Aldrich, St. Louis, USA) and the in vitro kinase reactions were carried out as described earlier (Huesmann et al. ...
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bioRxiv - Plant Biology 2020Quote: OsDMI3-GST fusion protein was produced in Escherichia coli Rosetta (DE3; Novagen), and tested for interaction as described previously (Ni et al. ...