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Citations for Millipore Sigma :
4651 - 4700 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were transferred to an Immobilon P membrane (EMD Millipore, IPVH00010) at 25°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... the proteins were eluted with 40 µl 3x FLAG peptide (Sigma-Aldrich) and collected by centrifugation for 30 min at 100xg.
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bioRxiv - Cancer Biology 2021Quote: ... separated protein bands were transferred into polyvinylidene fluoride membranes (Millipore, Cat. #IPVH00010). Then ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... Protein was wet transferred to polyvinylidene fluoride (PVDF) membranes (Immobilon P, Millipore) in Criterion blotter cells (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Cell Biology 2020Quote: ... otherwise all other protein labelling were using 1% Triton X100 (Sigma-Aldrich) in PBS permeabilization for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... followed by the addition of 5 mg protein A beads (Sigma, P1406) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... 20µl of DBX-washed Magna ChIP Protein W+G magnetic beads (Millipore) were added to each sample and rotated for 1hr at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Protein concentration of tissue lysates was determined using bicinchoninic acid (Sigma-Aldrich). Proteins were denatured with gel loading buffer (50 mM Tris [pH 6.8] ...
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bioRxiv - Microbiology 2021Quote: ... Protein complexes were purified with α-FLAG M2 affinity gel (Sigma-Aldrich) as described for co-IP ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were electroblotted to polyvinylidene fluoride membranes (IPFL00010, EMD Millipore, Billerica, MA), incubated with primary antibodies (see Supplemental Table 1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Proteins were visualized after staining with ProteoSilver Silver Stain Kit (Sigma-Aldrich) (S.Fig.1 ...
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bioRxiv - Immunology 2020Quote: ... and 50 μl of horseradish peroxidase-Staphylococcal protein-A conjugate (Sigma-Aldrich) diluted 1:3000 was added ...
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bioRxiv - Developmental Biology 2020Quote: ... Proteins were transferred to an Imobilon-PPVDF membrane (Millipore, Billerica, MA, IPVH00010). Membranes were probed with rabbit anti-chick RUNX2 primary antibody (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich) and then purified with Superdex 200 pg (GE Healthcare) ...
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bioRxiv - Pathology 2022Quote: ... Target proteins were detected using ECL western blot detection solution (Millipore, WBKLS0500). Primary antibodies used for immunoblotting were as follows ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Neuroscience 2021Quote: ... Guanine nucleotide-binding protein subunit alpha-11 (1:1,000; Millipore 05-855R), Guanine nucleotide-binding protein G(q ...
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bioRxiv - Neuroscience 2021Quote: ... we used the EZ-Magna RIPTM RNA-Binding Protein Immunoprecipitation Kit (Millipore) following the manual with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich), using BSA as a standard and aliquots maintained at −80°C until required ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-Ubiquitinylated proteins,clone FK2 (cat# 04-263, Merck Millipore; 1:1000). Ponceau solution was prepared with Ponceau BS (cat# B6008 ...
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bioRxiv - Molecular Biology 2021Quote: ... The separated proteins were transferred onto PVDF membrane (Millipore, Billerica, Massachusetts, USA) using phosphate based transfer buffer (10 mM sodium phosphate monobasic ...
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bioRxiv - Molecular Biology 2021Quote: ... The eluted protein was then precipitated using 100% trichloroacetic acid (Sigma-Aldrich) added drop wise to 20% of the final elution followed by vortexing and incubation at 4°C overnight prior to pelleting at 21130 x g for 20 min at 4°C ...
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bioRxiv - Biophysics 2022Quote: p53 and E2C proteins were labeled with fluorescein isothiocyanate (FITC) (Sigma-Aldrich), adapting the manufacturer’s protocol to obtain sub-stochiometric labeling enough to visualize the samples by fluorescence and confocal microscopy ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma, F7252) and imaged on ImageQuant LAS 4000 or Amersham ImageQuant 800 (GE Healthcare Life Sciences ...
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bioRxiv - Genomics 2020Quote: ... Protein loading was checked by staining membranes with Ponceau staining solution (Sigma) for 2min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... Cells were pelleted in Eppendorf® protein LoBind tubes (Sigma Aldrich Z666505) washed twice with ice cold PBS and snap-frozen ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were transferred to 0.2 μm pore-size nitrocellulose membranes (Millipore) and blocked in PBS with 0.1% (v/v ...
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bioRxiv - Biochemistry 2019Quote: ... All protein constructs were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with a Tobacco Etch Virus (TEV ...
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Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The protein yield was estimated using the Bradford assay (Sigma-Aldrich, USA) with BSA as the standard ...
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bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
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bioRxiv - Developmental Biology 2019Quote: Protein was extracted using 1X RIPA lysis buffer (Millipore Sigma, Burlington MA) supplemented with Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... immunoreactive proteins were detected by an enhanced chemiluminescence detection system (EMD-Millipore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The separated proteins were then transferred to PVDF membranes (0.45μm, Millipore, USA), and block with 5% milk for 1h ...
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bioRxiv - Immunology 2019Quote: ... The protein bands were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using a microplate Bradford (Sigma –Aldrich Bradford Reagent) protein assay with bovine serum albumin standards.
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bioRxiv - Cell Biology 2020Quote: ... we use the PureProteome Protein G Magnetic Bead System (EMD Millipore Corp.). 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using the bicinchoninic acid assay (BCA) kit (Sigma).
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bioRxiv - Biochemistry 2019Quote: ... the reconstituted protein was mixed with 50 μM ADP-Ribose (Sigma-Aldrich) and further purified on a Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... proteins were visualized by staining with Amido Black staining solution (Sigma-Aldrich) according to manufacturer’s directions.
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bioRxiv - Genetics 2019Quote: ... and then captured by protein G-agarose/salmon sperm DNA (Millipore Sigma). Precipitated DNA was reverse cross-linked and then amplified by qPCR using primers amplifying amplicons with the following midpoints relative to the transcription start site of Shox2 ...
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bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
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bioRxiv - Cell Biology 2019Quote: ... Separated proteins were then transferred to a PVDF membrane (IPVH00010, Millipore Corp.) for overnight at 4°C in a wet transfer system (Hoefer ...
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bioRxiv - Biochemistry 2019Quote: ... whole cell lysates (WCLs) were precleared using protein A Sepharose (Sigma-Aldrich) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Proteins were resolved by SDS-PAGE and transferred to PDVF membranes (Millipore) before immunoblotting with anti-HA (12CA5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein samples were treated with 10 mM dithiothreotol (DTT) (Sigma, MO) at 56 °C for 1 h and 55 mM IAA (Sigma ...
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bioRxiv - Plant Biology 2019Quote: ... 0.2% Igepal CA-630 and Protein Inhibitor Cocktail (Sigma-Aldrich; http://www.sigmaaldrich.com/). The cell suspension was frozen in liquid nitrogen and then thawed on ice and centrifuged for 5 min at 150 g ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Electrophoresis proteins were transferred to PVDF membranes (PVDF membrane, 0.45μm; MILLIPORE, USA) using a Bio-Red Laboratories Instruments Mini Trans-Blot Electrophoretic Transfer Cell unit (Alfred Nobel Drive Hercules ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...