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Citations for Millipore Sigma :
4501 - 4550 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... The PROX1-FLAG protein was then purified on M2 agarose (Sigma), which captures FLAG-tagged proteins as described previously 52 ...
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bioRxiv - Cancer Biology 2024Quote: ... Separated proteins were transferred onto a PVDF membrane (IPVH00010, Millipore Sigma) by wet-protein transfer ...
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bioRxiv - Cell Biology 2024Quote: Proteins were extracted from cell pellets resuspended in RIPA buffer (Sigma) containing cOmpleteTM Mini Protease Inhibitor Cocktail (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by phenol:chloroform:isoamyl alcohol (25:24:1) (PCI) (Sigma) extraction followed by ethanol precipitation ...
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bioRxiv - Cell Biology 2024Quote: ... Bound proteins were eluted with 5μg/ml FLAG peptide (Sigma, F3290) in buffer and subsequently separated on 4-12% NuPAGE Novex Bis-Tris Minigels (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were then transferred onto Immobilon-FL membrane (Millipore Cat. #IPFL85R) using transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to a PVDF membrane (Millipore Immobilion-P, IPVH00010) using a wet transfer with freshly made transfer buffer (25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... For labeling an Atto 488 protein labeling kit (Sigma-Aldrich, 38371) and an Atto 647N protein labeling kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... The protein was concentrated on Amicon Ultracel 10K centrifugal filters (Millipore) to 23 mg/ml concentration.
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bioRxiv - Neuroscience 2024Quote: ... Protein fragments were expressed in Escherichia coli BL21 Rosetta (DE3) (Novagen) cells ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in gradient Bolt Bis-Tris gels (Thermo) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein expression was induced with isopropyl-b-D-thiogalactopyranoside (IPTG, Millipore) at 100 uM and cells were grown overnight at 16°C ...
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bioRxiv - Cancer Biology 2024Quote: Protein lysates were harvested with RIPA lysis buffer (Sigma Aldrich; R0278) containing protease inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2024Quote: Proteins were isolated from the liver using CelLyticTM MT (Sigma, C3228) in the presence of a protease inhibitor/phosphatase inhibitor cocktail (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Neuroscience 2024Quote: ... the proteins were transferred to Immobilon-P PVSF (0.45µm, Sigma-Millipore) in Tris-Glycine buffer supplemented with 20% methanol for 1 h at 75V ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein was transferred to Immobilon-FL PVDF membrane (Merck Millipore, IPFL00010) at 100 volts using 1x Towbin transfer buffer with 20% methanol ...
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bioRxiv - Molecular Biology 2024Quote: ... and Etanercept chimeric proteins (Millipore Sigma Cat No. 185243-69-0), representing the extracellular domain of the target receptor fused to the Fc region of immunoglobulin G ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were transferred to a membrane (Immobilon-FL PVDF, IPFL00010, Millipore), stained with Revert 700 Total Protein Stain (926-11011 ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were transferred to polyvinylidene fluoride (PVDF) membranes (Merk-Millipore, IPFL00010), blocked with SuperBlock Blocking Buffer in PBS (Thermo Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... the FLAG interacting protein mix was eluted by 3xFLAG peptides (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... The proteins were transferred to polyvinylidene fluoride membrane (Millipore, Cat# ISEQ00010) following by incubation with blocking buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... Equal protein loading was confirmed using Ponceau S Staining (P7170, Sigma). Blots were stained using indicated antibodies (Supplementary Table 2) ...
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bioRxiv - Pathology 2024Quote: ... Transfer the proteins onto a PVDF membrane (ISEQ00010, Merck Millipore, Germany). Block the membrane with 5% skim milk for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... we diluted fluorescently tagged BSA protein (Sigma-Aldrich, St. Louis, MO) to a concentration of 0.1 mg/mL and encapsulated the solution in 45-μm droplets using the single-emulsion droplet-generation chip ...
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bioRxiv - Biochemistry 2024Quote: ... A dilution series of the protein synthesis inhibitor cycloheximide (Sigma 01810) was created in water ...
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bioRxiv - Biochemistry 2024Quote: Fusion proteins were expressed in BL21(DE3)pLysS E.coli (EMD Millipore). The proteins were tagged at the N-terminus with his6-HT7 (pET30-HT7 vector) ...
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bioRxiv - Bioengineering 2024Quote: Proteins were extracted from fibroblast cells using RIPA Buffer (Sigma #R0278) with protease inhibitors (Roche #11836153001) ...
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bioRxiv - Molecular Biology 2024Quote: ... The concentrated protein underwent further processing with centrifugal filter units (Millipore). The purified SsCBE2-C2 protein was then stored at -80°C.
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bioRxiv - Bioengineering 2024Quote: ... Protein concentration was calculated following Bradford assay (Bradford reagent, Sigma-Aldrich). A 1:50 mixture of β-mercaptoethanol (Acros Organics ...
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bioRxiv - Microbiology 2020Quote: ... Antibody bound to GP was then detected with anti-human antibody conjugated to HRP (EMD Millipore, Burlington, MA) and Ultra-TMB substrate (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... after which slides were washed in phosphate-buffered saline and stained against Human Nuclei (MAB4190, 1:250, Millipore) and Human specific Ki67 (MAB4383 ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
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bioRxiv - Cell Biology 2020Quote: Senescence in normal human fibroblasts was induced using five treatments with 30 μM tert-butyl hydroperoxide (tBHP, Sigma) for 5 consecutive days (1 h/day ...
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bioRxiv - Immunology 2021Quote: ... were added to either tissue culture-treated plates coated with 10 μg/ml human plasma fibronectin (Sigma-Aldrich) or Corning® Costar® Ultra-Low Attachment plates (Sigma-Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... mice underwent the full superovulation procedure with an intraperitoneal injection of 10 IU human chronic gonadotropin (hCG, Sigma) 48 hours after PMSG administration ...
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bioRxiv - Bioengineering 2019Quote: ... or standard 6-well tissue culture plastic dishes were incubated with human plasma fibronectin (Millipore Sigma, Catalog #FC010) at a concentration of 10 μg/ml diluted in PBS (3 ml/well of ECM protein solution added ...
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... human HT-1080 fibrosarcoma cells (CCL-121; ATCC) were maintained using Eagle’s Minimum Essential Medium (EMEM; Millipore Sigma) supplemented with 2 mM L-glutamine and containing 10% (vol/vol ...
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bioRxiv - Pathology 2020Quote: ... and immunostained with human monoclonal anti-CRP antibody (C1688; Sigma-Aldrich, Saint Louis, MO, USA; 1:400 dilution) which specifically detect the 24-kD monomeric CRP epitope[31 ...
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bioRxiv - Genomics 2019Quote: ... we measured 41 different cytokines and chemokines using the Milliplex MAP Human Cytokine/Chemokine Magnetic Bead Panel (Millipore kit no ...
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bioRxiv - Biochemistry 2021Quote: ... reactions were then incubated in the presence of 50 μg/mL (~0.1 units) human salivary α-amylase (Sigma) or 1.5 M hydroxylamine for a further 60 min at 37°C ...
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bioRxiv - Genetics 2021Quote: ... AG1-0 cells were passaged according to the product manual for PluriSTEM Human ES/iPS Medium (Millipore Sigma), which is feeder-free and utilizes Dispase II (Millipore Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse monoclonal antibodies against human LMα3 (clone CL3112) and mouse IgG (both Sigma-Aldrich, St. Louis, Missouri, USA) were used at 0.5 μg mL−1.
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bioRxiv - Cancer Biology 2021Quote: ... and cultured in Human IntestiCult™ Organoid Growth Medium (StemCell) supplemented with 10 mmol/L Y-27632 (Sigma), 5 mmol/L CHIR99021 (ReproCell ...
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bioRxiv - Neuroscience 2021Quote: ... the eye cup was treated for ∼15 minutes with human plasmin (∼50 μg/mL, Sigma or Haematologic Technologies) to aid vitreous removal ...
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bioRxiv - Cell Biology 2019Quote: ... either directly or after coating with 10 µg/ml human plasma fibronectin (cat no. FC010; Millipore, Billerica, MA), as noted in the figure legend.
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bioRxiv - Biochemistry 2020Quote: DNA sequences containing human SUN2 were cloned into a modified bicistronic ampicillin resistant pETDuet-1 vector (EMD Millipore), superfolder GFP-KASH fusions (sfGFP ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were incubated with the respective primary antibody (rabbit anti human, anti-ANK1 or anti-EPB41, Sigma-Aldrich) overnight ...
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bioRxiv - Molecular Biology 2021Quote: Nuclei from human liver (15 mg) were isolated using with the Nuclei EZ Prep kit (Sigma-Aldrich, #NUC101) and a glass dounce homogenizer (25 strokes with lose pastel and 25 strokes with tight pastel in ice-cold Nuclei EZ Lysis Buffer) ...