Labshake search
Citations for Millipore Sigma :
4951 - 5000 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The pooled protein was concentrated using a centrifugal concentrator (Millipore, 100 kDa) prior to injection onto a Superdex 200 Increase 10/300 GL (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... A mixture consisting of rCas9NLS protein (0.66 mg/ml, Sigma-Aldrich CAS9PL), Phenol red (0.05% ...
-
bioRxiv - Cancer Biology 2024Quote: ... The separated proteins were transferred to a PVDF membrane (Millipore, 0.2 μm). The membrane was blocked in 5% nonfat dry milk (Biofroxx ...
-
bioRxiv - Cancer Biology 2024Quote: ... as per manufacturer instructions and 0-5µg/mL OVA protein (Millipore Sigma) for 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined by BCA assay (Sigma Aldrich, cat no: B9643). 30-40 μg protein was loaded in Mini-PROTEAN® Tetra Cell Systems and TGX™ precast gels ...
-
bioRxiv - Molecular Biology 2024Quote: ... Rabbit polyclonal antibody against protein disulfide isomerase (1:100) (PDI; P7122, Sigma). Secondary antibody Alexa Fluor 555 (A-21422 ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein bands were visualized with Immobolin Forte Western HRP substrate (Sigma, WBLUF0100) and imaged with a ChemiDoc Imaging System (BioRad) ...
-
bioRxiv - Neuroscience 2024Quote: ... rabbit anti-amyloid precursor protein (APP, C-terminal, 1:1000, Sigma, #A8717), rat anti-green fluorescent protein (GFP ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were detected using an enhanced chemiluminescent detection system (EMD Millipore, #WBKLS0500) and ChemiDoc Imaging System (Bio-Rad Laboratories ...
-
bioRxiv - Neuroscience 2024Quote: ... Mouse Anti-Glial Fibrillary Acidic Protein (1:100, Millipore, Cat# MAB360, RRID:AB_11212597), Mouse Anti-NeuN (1:1000 ...
-
bioRxiv - Pathology 2024Quote: ... and the blotted protein bands were revealed by the ECL system (Millipore).
-
bioRxiv - Genetics 2024Quote: ... Captured protein was detected by immunoblotting with anti-FLAG M2 antibody (Sigma) and visualized using an Odyssey Infrared Imaging System (Licor) ...
-
bioRxiv - Microbiology 2024Quote: ... The procedure involved reducing the proteins with 10 mM DTT (Sigma-Aldrich), followed by carbamidomethylation with 100 mM iodocetamide (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bead-bound proteins were eluted with 200 ng/µL 3ξFLAG peptide (Sigma) by incubating at 4°C for 30 min ...
-
bioRxiv - Biochemistry 2023Quote: ... and protein was visualized using Immunobilon Western Chemilumiescent HRP Substrate (Millipore, WBKLS0500) on an ImageQuant LAS 4000 (GE Healthcare) ...
-
bioRxiv - Biochemistry 2023Quote: ... protease incubation (Pepsin Porcine, 1:50, enzyme to protein ratio, Sigma-Aldrich) was performed for 4 h at 37 °C in 40 mM HCl in a total volume of 2 ml (pH 2) ...
-
bioRxiv - Biochemistry 2023Quote: ... Gels were stained using ReadyBlue protein gel stain (Sigma-Aldrich, RSB-1L) followed by de-staining with water ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010 ...
-
bioRxiv - Immunology 2023Quote: ... The protein in PBS was 0.22um filtered using spin filters (Millipore Sigma) prior to Size exclusion Chromatography (SEC) ...
-
bioRxiv - Cancer Biology 2024Quote: ... for FLAG immunoprecipitation or Protein A agarose beads (EMD Millipore 16-125) for LONP1 immunoprecipitation on a rotating platform at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... we precipitated the eluted proteins by adding trichloroacetic acid (#T0699, Sigma Aldrich) to 20% final concentration ...
-
bioRxiv - Biophysics 2024Quote: ... BCA Protein Assay Kit and oligonucleotides were purchased from Sigma Aldrich (international). Low melting temperature agarose was purchased from Fisher Bioreagents (Massachusetts ...
-
bioRxiv - Neuroscience 2023Quote: ... the proteins were reduced with 10mM tris(2-carboxyethyl)-phosphine (TCEP) (Sigma) for 20 min ...
-
bioRxiv - Cell Biology 2023Quote: ... Transferred proteins were then stained with Ponceau S Solution (Sigma-Aldrich P3504). Blots were blocked (5% (w/v ...
-
bioRxiv - Physiology 2023Quote: ... Proteins were detected based on HRP substrate-based enhanced chemiluminescence (#WBKLS0500, Millipore), visualized using ChemiDoc Touch Imaging System (BioRad ...
-
bioRxiv - Biochemistry 2022Quote: ... then stained with ReadyBlue™ Protein Gel Stain from Sigma-Aldrich (USA) and imaged and analyzed.
-
bioRxiv - Molecular Biology 2022Quote: Total proteins were extracted from cells using RIPA buffer (Sigma-Aldrich, #R0278) and quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies used for probing for specific proteins were: actin (Sigma A-2066), Hsp70 (Thermo-Fisher MA 3006) ...
-
bioRxiv - Cell Biology 2023Quote: ... Rab proteins were either loaded with GDP (REP-complexes, Sigma Aldrich, Germany) or MANT-GDP (GDI-complexes ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore, Darmstadt, Germany), blocked with 5% dry milk in TBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... protein lysates were separated and transferred onto polyvinylidene fluoride membranes (Millipore, USA). After being blocked with five-percent non-fat dry milk in Tris-buffered saline Tween-20 (TBST ...
-
bioRxiv - Microbiology 2022Quote: ... All lysed protein samples were incubated with anti-Flag magnetic beads (Sigma) at 4°C overnight ...
-
bioRxiv - Immunology 2023Quote: ... H3K27me3 or HK3K27Ac ChIP-grade antibodies and Protein A magnetic beads (Millipore). FAIRE was performed on samples fixed and sonicated as per ChIP ...
-
bioRxiv - Systems Biology 2023Quote: ... Proteins were digested overnight at 37C with 1:50 trypsin (Sigma-Aldrich) and 1:75 and lysyl endopeptidase (Wako) ...
-
bioRxiv - Genetics 2023Quote: ... Proteins were transferred to a PVDF membrane (Immobilon-FL, Millipore, Burlington, MA) and blocked for 1 h at room temperature in Odyssey blocking buffer (Li-Cor ...
-
bioRxiv - Biochemistry 2023Quote: ... Antibody precipitated with 100 µL Protein A/G beads (Millipore, IP05-1.5ML) was washed twice in 700 µL DRIP buffer ...
-
bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were then transferred to PVDF membrane (Immobilon, Millipore, Billerica, MA, USA) by semi-dry blotting ...
-
bioRxiv - Cell Biology 2023Quote: Equal amounts of protein samples were supplemented with β-mercaptoethanol (Sigma; M6250) and diluted in 1X Laemmli Sample buffer (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... Polyclonal antibodies from antiserum were purified using Protein A Sepharose (Sigma, USA) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2023Quote: ... and the proteins transferred onto polyvinylidene fluoride membrane (Immobilion-P, Millipore, USA) according to manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were incubated with 20 μl protein G-coated Sepharose (Sigma-Aldrich) previously conjugated with 5 μl of anti-FLAG antibody by overnight incubation ...
-
bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by immunoblotting using anti-Glutathione-S-Transferase (Sigma-Aldrich), anti-polyHistidine-Peroxidase (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein detection were performed with Immobilon Western Chemiluminescent HRP Substrate (ECL, Millipore). Band intensities were analyzed by ImageJ software.
-
bioRxiv - Biophysics 2023Quote: ... The protein was further concentrated by a 100-kDa cutoff concentrator (Millipore) and loaded onto a Superose 6 increase 10/300 column (GE Healthcare ...
-
bioRxiv - Biochemistry 2023Quote: ... equal amounts of proteins were then incubated with anti-Flag Beads (Sigma), anti-Myc Beads (Sigma ...
-
bioRxiv - Neuroscience 2023Quote: ... and the protein pellet resuspended and incubated in 2M hydroxylamine (NH2OH) (Sigma), pH 7.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were precipitated with 5x ice cold acetone (HPLC grade, Sigma-Aldrich) and centrifuged at 20,627 g for 10 min at 4° C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were visualised using Immobilon Classico Western HRP substrate (Sigma-Aldrich, WBLUC0100). Primary antibody dilutions are as follow ...
-
bioRxiv - Neuroscience 2023Quote: ... The dialyzed protein solution was filtered (0.45 μm pore size filter, Millipore) and protein concentration was determined by absorbance measurements at 280 nm using extinction coefficient ε = 37752 M-1cm-1 ...