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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and 1 μM 4–hydroxytamoxifen (4–OHT) (H7904–5MG, Sigma) for 3 hr to induce mCherry–FokI expression and 1 mg/ml doxycycline hyclate (D9891–G ...
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bioRxiv - Synthetic Biology 2020Quote: ... (Z)-4- hydroxytamoxifen (4-HT, H7904, Sigma-Aldrich, 10 mM), 3,3’,5-triiodo-L-thyronine (T3 ...
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bioRxiv - Neuroscience 2020Quote: ... or 4% paraformaldehyde/4% sucrose/1% TritonX-100 (Sigma-Aldrich), as described in (62 ...
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bioRxiv - Physiology 2023Quote: ... and 4-aminopyridine (4-AP) were obtained from Sigma-Aldrich. We used standard locust saline to prepare all pharmacological agents ...
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bioRxiv - Neuroscience 2023Quote: ... 10mg of 4-hydroxytamoxifen (4-OHT, Sigma Aldrich #H6278-10mg) was added with 500μl ethanol and shaken at room temperature until the powder was completely dissolved (20mg/ml) ...
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bioRxiv - Neuroscience 2024Quote: ... 4-hydroxy-tamoxfen (4-OHT) was purchased from Sigma (H6728) and was dissolved in 100% DMSO and stored in the -20 until use ...
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bioRxiv - Neuroscience 2024Quote: Recombination was induced with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich), as previously reported [6,11] ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-Methylumbelliferyl sulfate (4-MUS) substrate was from Sigma-Aldrich and GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tamoxifen (4-hydroxytamoxifen, 4-OHT) was obtained from Sigma (#H6278) and was reconstituted in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... and 2.5 μM 4-hydroxytamoxifen (4-OHT) (Sigma, Cat#H7904) to shuttle C/EBPα into the cell nucleus.
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bioRxiv - Cancer Biology 2022Quote: 10 mg/ml of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) was dissolved in acetone with gentle heating ...
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bioRxiv - Neuroscience 2023Quote: ... The 4-OHT (4-OHT; Sigma, MO, USA Cat# H6278) was dissolved in ethanol to create a 20mg/ml stock solution ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 4% paraformaldehyde (4% PFA; Sigma-Aldrich, cat# P6148) in 1x PBS pH 7.4 ...
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bioRxiv - Bioengineering 2024Quote: ... For the calibration of 4-methylumbelliferone (4-MU, Sigma-Aldrich), we prepared solutions of 4-MU in concentrations of 1000 ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Genomics 2020Quote: Human melanoma cells were seeded in 12 well plates with increasing volumes of lentiviral supernatant in a final volume of 2 mL with 4 μg/mL polybrene (Millipore, Burlington, MA). Plates were centrifuged for 2 hours at 1,000x g at 30°C and cells were incubated for 16 hours at 37°C and 5% CO2 in a humidified incubator ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Systems Biology 2022Quote: ... the pellicles were first digested by shaking for 16 h at 4 °C in 15 ml of PBS buffer with 2% cellulase (Sigma Aldrich, C2730).
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bioRxiv - Neuroscience 2021Quote: ... The supernatant became the soluble fraction and the pellet was solubilized in urea buffer (30 mM Tris, 7 M Urea, 2 M Thiourea, 4% CHAPS, 1X Protease Inhibitor Cocktail (Millipore Sigma 11873580001), 0.5 mM PMSF ...
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bioRxiv - Microbiology 2020Quote: Zn2+release from DksA was measured using the metal chelator 4-(2-pyridylazo)resorcinol (PAR) (Sigma-Aldrich, St. Louis, MO, United States) (59) ...
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bioRxiv - Biochemistry 2020Quote: ... Unbound dye was removed by 2 washes with 100X excess of protein storage buffer followed by centrifugation in Amicon® Ultra-4 Centrifugal Filter Units (SIGMA MilliPORE). LLPS of dyed and undyed protein was compared as quality control and results were similar (data not shown).
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bioRxiv - Molecular Biology 2020Quote: ... Table 2) and were introduced by 4 lentiviral infections at 12hr intervals in presence of 8 μg/ml polybrene (Sigma-Aldrich®) using supernatant from transfected 293T cells ...
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bioRxiv - Immunology 2022Quote: ... the retrovirus supernatant was concentrated by centrifugation at 5000g at 4 °C for 2 h with Amicon Ultra-15 Centrifugal Filter Units (Millipore, Billerica, MA). A human T cell line (Jurkat ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked for 4 hrs at 4°C, incubated with primary antibodies for Isl1/2 (mouse, DSHB 39.4D5, 1:500) and Peripherin (rabbit, Millipore AB1530, 1:200) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... For immunostaining cells were fixed with 4% PFA in cytoskeleton preservation buffer (10mM 2-(N-morpholino) ethanesulfonic acid (MES, Sigma-Aldrich, M3671) pH 6.1 ...
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bioRxiv - Neuroscience 2020Quote: ... After rinsing three times for 10 minutes in TBS at RT sections were incubated with 4?,6-Diamidin-2-phenylindol (Dapi, Sigma, Cat# D9542) for 10 minutes at RT and washed once for 10 minutes in TBS ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Biophysics 2023Quote: ... with a voxel-size of 0.636 × 0.636 × 2 μm) are recorded once before and 4 h after actin-depolymerization using 10 μM cytochalasin D (Sigma-Aldrich, St. Louis) using brightfield and second-harmonic generation imaging microscopy ...
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bioRxiv - Genomics 2023Quote: ... 160 μl of 2x lysis buffer (2 % Triton x-100, 100 mM Tris-HCl pH 8.0, 300 mM NaCl, 0.2% sodium deoxycholate (Sigma-Aldrich, 302-95-4), 10 mM sodium butyrate (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... were coated overnight with retronectin solution (32 µg/mL in PBS, WEHI) at 4℃ followed by blocking with 2% bovine serum albumin solution (Sigma-Aldrich, #A1595) in PBS at 37℃ for 30 min prior to coating with viral supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were induced with 2-4% isoflurane and injected with 15 µL of CFA (Sigma-Aldrich F5881. Sigma, St. Louis, MO, USA) subcutaneously in the snout ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells exposed to glutamine starvation were also treated with a freshly made 2 mM methionine sulfoximine (MSX, Sigma Aldrich 15985-39-4). The TCA precipitated samples were then centrifuged at 4000 rpm for 5 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were incubated with fluorescent dye-conjugated secondary antibodies with 1% NDS in PBST with 4’,6-diamidino-2-phenylindole (DAPI, 1:1000, Sigma-Aldrich, D9542) at RT for 2 hours ...
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bioRxiv - Immunology 2024Quote: ... counted and 2-4×106 cells were washed twice in ice cold 0.9% saline made with NaCl (Sigma Aldrich, 31434-500G-M) and ultrapure HPLC grade water (Alfa Aesar ...
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bioRxiv - Cell Biology 2022Quote: Cultured cells were fixed with 4% paraformaldehyde (PFA) and 2% sucrose and then stained with Nile red (Sigma-Aldrich, St. Louis, MO) and HCS Cell Mask Blue (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell nuclei and actin filaments were stained simultaneously with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI; 1:2000, Sigma-Aldrich, Cat# D9542) and Phalloidin-Atto 647N (1:2000 ...
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bioRxiv - Biochemistry 2022Quote: ... EGTA-eluates were pooled and concentrated to a final concentration of ∼2 mg/ml using an Amicon Ultra-4 cellulose centrifugal filter unit (Merck Millipore, Cat# UFC810024).
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bioRxiv - Molecular Biology 2023Quote: ... the cells were incubated for two hours with Alexa Fluor® 647 labeled secondary antibody (1:500), and nuclei were labeled with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Sigma-Aldrich, #D8417). The fluorescent images were obtained with a Zeiss Axio Observer inverted microscope (40 × NA 1.3 oil objective ...
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bioRxiv - Biochemistry 2022Quote: ... sodium hydroxide (1310-73-2) and α-amylase (DFP Treated, Type I-A, saline suspension, 647-015-00-4) were purchased from Sigma-Aldrich Company Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... An amount of 2-4 mg of whole cell extract as incubated with 25 μL ANTI-FLAG® M2 affinity gel (Millipore Sigma) overnight at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Samples were cryoprotected in a 30% sucrose solution (w/v) overnight at 4°C and frozen in 2-methylbutane (Sigma-Aldrich 106056) on dry ice ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated 2 hr at 4°C with rotation after addition of either 6 μg mouse αSFPQ (Sigma-Aldrich WH0006421M2, RRID:AB_1843565)) or 6 μg normal mouse αIgG (Santa Cruz sc-2025 ...
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bioRxiv - Neuroscience 2024Quote: ... The β- hexosaminidase assay was performed in a 96-well plate by mixing 50 μL of 2 mg/mL 4-nitrophenyl N-acetyl-β-D-galactosaminide (N9003, Millipore Sigma) in 0.1 M citrate buffer (pH 4.5 ...
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bioRxiv - Plant Biology 2024Quote: ... WTN1 Δ 23-HA and PWT4-HA proteins were incubated at 4°C for 2 h to capture Flag-tagged proteins using Flag-Trap magnetic beads (Sigma-Aldrich, M8823). Following incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... at 30 °C The lysate solution was added 2.5 μL 800 mM tris(2-carboxyethyl)phosphine (ALDRICH®) and 5 μL 100 mM iodoacetamide (SIGMA®) to in PCT-Micro tube to dilute into a final concentration of 10 mM and 40 mM ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one received henceforth 2 mg/ml DOX (BelaDox, Bela-pharm) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce TCF7L1 re-expression (DOX (+)) ...
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bioRxiv - Cancer Biology 2021Quote: ... mice were randomized in two groups of which one was henceforth treated with 2 mg/ml DOX (Beladox, Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo re-expression (DOX (+)) ...
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bioRxiv - Cell Biology 2020Quote: ... Carbonyl cyanide α-[3-(2-benzothiazolyl)6-[2-[2-[bis(carboxymethyl)amino]-5-methylphenoxy]-2-oxo-2H-1-benzopyran-7yl]-b-(carboxymethyl)-tetrapotassium salt (FCCP, cat. n. C2920, Sigma-Aldrich) was solubilized in ethanol to a final stock concentration of 10 mM ...
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bioRxiv - Genomics 2021Quote: ... PTA was carried out by first further lysing the cells after freeze thawing by adding 2 μL a prechilled solution of a 1:1 mixture of 5% Triton X-100 (Sigma-Aldrich) and 20 mg/ml Proteinase K (Promega) ...