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Citations for Millipore Sigma :
4701 - 4750 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the medium was replaced with medium containing the corresponding treatments and 6h a pulse of BrdU (5-bromo-2-deoxyuridine, 50 µM; Sigma-Aldrich) was given to the neonatal cells ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 2.5 h at 37°C and 5% CO2 in 2% FBS DMEM supplemented with 100 µg ml-1 cycloheximide (#C4859, Sigma-Aldrich) to prevent viral proteins synthesis ...
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bioRxiv - Genetics 2023Quote: ... Cells were washed three times with PBS for 5 minutes followed by DAPI staining (2 µg/ml in PBS, Sigma, D9564) for 5 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... was dissolved in isopropanol:chloroform (2:1) and added in small aliquots to a stirring 5% (w/v) methyl-β-cyclodextrin (Sigma-Aldrich) solution in a water bath heated to 80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Molecular Biology 2024Quote: ... or procyclic forms were collected from cultures and fixed for 5 min at room temperature with 2% of formaldehyde (F8775, Sigma-Aldrich). To stain the nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... Approximately 20 x 107 HeLaK cells pre- synchronized by thymidine treatment (2 mM for 18 h) were released to undergo S-phase in presence of F-ara-Edu (5 µM; Sigma-Aldrich) for 5 h ...
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Cellulose fermentation by the gut microbiota is likely not essential for the nutrition of millipedesbioRxiv - Microbiology 2024Quote: ... while the other two groups were fed litter treated with 5 mM or 10 mM of Sodium 2-bromoethanesulfonate (Na-BES; Sigma-Aldrich) to inhibit methanogenesis ...
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Synthetase and Hydrolase Specificity Collectively Excludes 2’-Deoxyguanosine from Bacterial AlarmonebioRxiv - Microbiology 2024Quote: ... Reactions were carried out at 42 °C for 1 hr and then treated 5 μmol sodium 2-mercaptoethanesulfonate (MESNa, Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... 5 x 105 MDCK cells per ml were prepared in serum free DMEM containing 2% v/v penicillin/streptomycin (Sigma-Aldrich) and 2 µg/ml TPCK Trypsin (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... the media from each well was aspirated and the wells were overlaid with 2 mL/well DMEM with 5% FBS and 1.2% Avicel® PH-101 (Sigma,11363). At 7 dpi ...
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bioRxiv - Cancer Biology 2023Quote: CRISPR-Cas13d or Tet-On overexpressing cells were sequentially pulse-labeled with 50 µM 5-chloro-2′-deoxyuridine (CldU, Sigma-Aldrich) and 250 µM 5-iodo-2′-deoxyuridine (IdU ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Bioengineering 2022Quote: ... Three infused hydrogel samples per condition were stacked in a 15×15×5 mm vinyl cryomold (Sakura) and flash frozen by submersion in liquid nitrogen-cooled 2-methylbutane (Sigma-Aldrich). Frozen samples were removed from the mold and mounted lengthwise onto a Leica CM1850 cryostat such that each cut would take a cross-section of the hydrogel stack ...
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bioRxiv - Developmental Biology 2022Quote: ... fish were incubated in freshly prepared tank water containing 30 mg/l of the halogenated thymidine analog 5-bromo-2’-deoxyuridine (BrdU; Sigma-Aldrich) for periods between 12 h and 24 h at various time points following experimental intervention and kept in the dark at 28°C ...
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bioRxiv - Developmental Biology 2022Quote: ... with 150mg/kg body weight of thymidine analogs of 5-bromo-2-deoxyuridine (BrdU) (Sigma Aldrich, cat# B9285, St. Louis, MO) or 5-Iodo-2-deoxyuridine (IdU ...
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bioRxiv - Immunology 2022Quote: In order to label mitotic cells with EdU, mice were injected intraperitoneally with thymidine analogue 5-Ethynyl-2′-deoxyuridine (EdU, 50 μg/g) (Sigma-Aldrich) 3 hours before sample collection ...
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bioRxiv - Cell Biology 2023Quote: ... the liquid culture phase was collected as a starter culture and diluted 1:100 in fresh BSFB (final volume of 2 L) supplemented with 5 µg/mL vancomycin (Sigma-Aldrich), and incubated on a shaker platform in a humidified ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded on a 18×24 mm coverslip and the next day they were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cell Biology 2024Quote: Organoids collected between day 16 to day 21 after replating (between passage 5 and 12) were washed with PBS and fixed with ice-cold glutaraldehyde 2% (Electron microscopy grade, Sigma #G5882) and post-fixed in 2% OsO4 in 0.1M cacodylate buffer (pH 7.2) ...
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bioRxiv - Biochemistry 2024Quote: ... then incubated for 1-2 h at room temperature in PBST with blocking agent (5 mg/mL Bovine Serum Albumin (Sigma Aldrich) for anti-Strep blots ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were subsequently blocked with 2% BSA-PBS for 1 hour at room temperature and then stained with 5 µU/mL of phalloidin 568 (Sigma-Aldrich) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell concentration and viability (propidium iodide-negative population) were measured after 5 min incubation of cell aliquot in 2 µg/ml propidium iodide (Sigma, 81845) using the portable flow cytometer Moxi Flow (ORFLO Technologies ...
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bioRxiv - Bioengineering 2024Quote: Isolated cells are spun down at 25G for 5 minutes and resuspended in 4C UW supplemented with 2 mg/mL BSA and 100 mM D-glucose (Sigma-Aldrich). 10% v/v DMSO is added to the suspension resulting in a final cell concentration of 10 million cells / 1.5 mL and incubated for 20 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... the medium was changed with “Day 2 medium” consisting of “base medium” supplemented with 5 μM XAV-939 (Sigma-Aldrich X3004), 5 ng/mL VEGF (R&D Systems 293-VE-050) ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μg of tissue lysate was incubated with 2 μl 0.5 M EDTA and either 5 μl 20 mM GDP (Sigma-Aldrich G7637) or 5 μl 20 mM GTPγS (Sigma-Aldrich G8634 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed for 3 minutes (5 times) with cold PBS before being blocked for 1 hr in fish Gel Blocking Buffer (2% Fish Gel [Sigma-Aldrich G741-100G] ...
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bioRxiv - Microbiology 2024Quote: ... The labelling and purification of single or double labelled HSV-1 virions with 5-Ethynyl-2’-deoxycytidine (EdC; Sigma-Aldrich, T511307) and 7-Deaza-7-ethynyl-2’-deoxyadenosine (EdA ...
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bioRxiv - Microbiology 2024Quote: ... co-cultures were fixed by adding an equal volume of solution containing 2% paraformaldehyde (PFA) (Thermo) and 5% glutaraldehyde (GA) (Sigma-Aldrich) in 0.1 M sodium cacodylate buffer (SCB ...
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bioRxiv - Microbiology 2024Quote: ... Salmonella were harvested at 4000 RCF for 15 minutes and resuspended in SDS-UREA (50mM Tris-HCl pH 6.8, 2% SDS, 6M UREA, 0.3% Bromophenol Blue) containing 5% β-Mercaptoethanol (Sigma-Aldrich, #M6250). To harvest supernatants ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 µM (NMDA receptor antagonist that preferentially binds to GluN2C/GluN2D (40)) or DL-2-Amino-5-phosphonovaleric acid (APV, Sigma, A5282) 100 µM (broad spectrum NMDA receptor blocker).
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bioRxiv - Neuroscience 2024Quote: ... in saline solution 2 ml of Evans blue albumin (EBA, 5% bovine albumin labelled with 1% Evans blue; molecular weight: 68 kDa, Sigma Chemicals) for 1 h.[2] Finally ...
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bioRxiv - Immunology 2024Quote: ... were seeded in 6-well plates (1.5×106 cells/well) with or without phorbol 12-myristate 13-acetate (PMA, 100 ng/ml, 2 ml/well, Sigma-Aldrich) and incubated at 37°C for 48 hrs ...
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bioRxiv - Neuroscience 2024Quote: ... GeneSwitch expression was induced transferring 2-to 5-days-old flies to vials containing food supplemented with RU486 (mifepristone, Sigma, USA) in 80% ethanol to a final concentration of 200 µg/ml for exactly 72 h prior to dissection ...
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bioRxiv - Cell Biology 2024Quote: Tissue for wholemount imaging was then cut into pieces of 1cmx1cm and cleared while shaking on 37°C in CUBIC-1A (5% N,N,N′,N′-Tetrakis(2-hydroxypropyl) ethylenediamine (122262, Sigma-Aldrich), 10% Urea (U5378 ...
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bioRxiv - Cell Biology 2024Quote: Intracellular ROS was measured in hMG exposed to 5 ng/mL NM for 24 hours using 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH2-DA) probe (Sigma-Aldrich), which turns highly fluorescent upon oxidation ...
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bioRxiv - Bioengineering 2024Quote: ... explants (n = 7-12/group) were washed for 2 hours and then digested with proteinase K (5 mg/mL) (Sigma-Aldrich) for 16 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsinized cells were spun down at 500 x g for 5 min and cell pellets were resuspended for 20 min in 2% (v/v) formaldehyde (Sigma, 252549) solution in PBS for fixation at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized in two groups of which one was henceforth treated with 2 mg/ml DOX (Beladox, Bela-pharm, Germany) dissolved in drinking water containing 5% sucrose (Sigma-Aldrich) to induce an in vivo re-expression (DOX (+)) ...
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bioRxiv - Bioengineering 2024Quote: ... Amicon Ultra-0.5 (PLBC Ultracel-3 membrane, 3 kDa) and Amicon® Ultra-15 Centrifugal Filters (3 kDa MWCO) were from Millipore (Tokyo, Japan). Calcofluor-white and imidazole were from Sigma-Aldrich (Tokyo ...
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bioRxiv - Neuroscience 2021Quote: ... and serotonin (5-HT, Sigma-Aldrich, Figure 5) or using blue light on the lumbar spinal cord of ChR2-expressing pups (Vglut2Cre;ChR2floxed ...
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bioRxiv - Physiology 2022Quote: ... 20 μM 5-fluorouracil (5-FU, Sigma-Aldrich) was added to prevent progeny growth ...
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bioRxiv - Biochemistry 2022Quote: ... 5% glycerol with addition 5 mM desthiobiotin (Sigma). For CENP-HIKM ...
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bioRxiv - Microbiology 2022Quote: ... 5-fluorouracil (5-FC, Sigma-Aldrich, cat#F6627) was prepared in drinking water at a final concentration of 2.0 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... and 5-deoxy-azacytidine (DAC-5, Sigma-Aldrich). In each case ...
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bioRxiv - Cancer Biology 2020Quote: 5-FluoroUracil (5-FU) (Sigma Aldrich, Milan, Italy) was used at different concentrations and for different time points based on the specific experiment ...
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bioRxiv - Developmental Biology 2022Quote: ... 5-hydroxy-L-tryptophan (5-HTP, Sigma-Aldrich); 3-hydroxybenzilhydrazine (NSD-1015 ...
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bioRxiv - Developmental Biology 2022Quote: ... serotonin (5-hydroxytryptamine hydrochloride, 5-HT, Sigma-Aldrich); L-tryptophan (tryptophan ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 nM 3,3’,5-triiodo-L-thyronine (Sigma), 1 μM dexamethasone (Sigma) ...