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Citations for Millipore Sigma :
4401 - 4450 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were irradiated using 8 J/m2 UV and labelled for 7 hours with 20 µM 5-Ethynyl-2’-deoxyuridin (EdU) and 1 µM Floxouridine (Sigma). Subsequently ...
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bioRxiv - Biophysics 2020Quote: ... both paraformaldehyde and methanol fixed cells are incubated with primary antibody diluted in the antibody dilution buffer (PBS supplemented with 5% FBS, 2% bovine serum albumin and 0.1% saponin (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduction was performed at a multiplicity of infection (MOI) of 2 in the presence of 5 µg/ml polybrene (Sigma). Cells were selected with 0.5-2.5 and µg/ml puromycin (Gibco) ...
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bioRxiv - Microbiology 2020Quote: A549 cells infected with arboviruses or ACE2-SK-N-SH cells infected with SARS-CoV-2 (MOI=0.05-5) were treated with the RIPK2 inhibitor GSK583 (28) (Sigma-Aldrich) for 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... but the slides had to be deparaffinized first with the following serial incubations: 2×5 minutes xylenes (Sigma, cat# 534056) twice ...
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bioRxiv - Neuroscience 2020Quote: ... washed 3 times with PBS and incubated for 2 h with blocking and permeabilization buffer (1% BSA, 5% goat serum, 0.15% saponin (Sigma Aldrich)) ...
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bioRxiv - Cancer Biology 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Microbiology 2021Quote: ... The matrix consisted of a 9:1 mixture of 2,5-dihydroxybenzoic acid and 2-hydroxy-5-methoxybenzoic acid (super-DHB, Sigma-Aldrich) at a final concentration of 10 mg/ml dissolved in chloroform ...
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bioRxiv - Systems Biology 2022Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described.62
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... washed in dilutions of SSC (2x and 0.2x) and TNT prior to blocking (1-2 hours in blocking solution of 5% heat-inactivated horse serum (Millipore Sigma), 0.5% Western Blocking Regent (Millipore Sigma) ...
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bioRxiv - Physiology 2021Quote: ... 0.5% Na-deoxycholate and 1% Triton X100) containing protease inhibitors and phosphatase inhibitor cocktail 1 and 2 (1:100, Sigma). Protein lysates were separated on a 4-15% TGX gel (Biorad ...
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bioRxiv - Plant Biology 2021Quote: ... 1.7 M sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1x protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]). The sample was centrifuged at 16,000 g at 4°C for 45 min ...
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bioRxiv - Biochemistry 2021Quote: Dried polar samples were resuspended in 100 μL water and 2 μL were injected into a ZIC-pHILIC 150 x 2.1 mm (5 μm particle size) column (EMD Millipore). Chromatographic separation was achieved using the following conditions ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Cell Biology 2021Quote: ... were resuspended in DPBS at 2 - 5 million cells per ml and incubated with 1 µl (25 - 29 U) benzonase (Millipore) at 37 °C for a minimum of 1 hr ...
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bioRxiv - Physiology 2020Quote: Excised hearts were placed in 1 μM Di-4-ANBDQPQ in Evans for 5 minutes and were placed in a dish containing 2% solution low melting point agarose (Sigma) dissolved in Evans solution ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... the elutions containing ORC (typically 2-5) were pooled and incubated with 40 µl of equilibrated Anti-V5-antibody-coupled Agarose Affinity Gel (Sigma) for 30 min at 4°C with rotation ...
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bioRxiv - Molecular Biology 2022Quote: ... Dried samples were resuspended in 25 μL water and 2 μL was inj ected into a ZIC-pHILIC 150 × 2.1 mm (5 μm particle size) column (EMD Millipore). operated on a Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The supernatant was dried by speedvac and reconstituted in 7.5 µL of 66% acetonitrile and 2 µL was separated by a ZIC□HILIC column (150 × 2.1 mm, EMD Millipore) coupled with a Q Exactive HF Orbitrap MS (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Day 25 differentiated kidney organoids were incubated with 10 µM of 5-Bromo-2’-deoxyuridine (BrdU) (Sigma-Aldrich, #B5002-100MG) in basal medium for 2 hours at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Exponentially growing RPE-1 (human retinal pigment epithelial) cells were pulse-labeled with CldU (5-Chloro-2’-deoxyuridine, Millipore Sigma) and IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were subsequently blocked for 2 h at room temperature with 5% bovine serum albumin (BSA) (Sigma-Aldrich, MN, USA) in Tris buffered saline (TBS ...
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bioRxiv - Neuroscience 2023Quote: Cell proliferation was assessed at DIV2 by co-incubating cells with 10μM of 5-bromo-2’-deoxyuridine (BrdU) (#B5002, Sigma-Aldrich) in the last 4h of the pharmacological treatments ...
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bioRxiv - Neuroscience 2023Quote: ... After blocking tissue with 5% swine serum and 2% BSA in permeabilizing conditions (0.1% Triton X-100, Sigma-Aldrich, # T8787), immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Physiology 2023Quote: ... were transferred to OP50-seeded plates or RNAi-seeded NGM plates supplemented with 50 μM of 5-fluoro-2-deoxyuridine (FUDR, Sigma) to prevent progeny growth ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed four to five times with warm media (DME-HG for MEFs and DME-HG/F12 for MCF10A) and pulse-labeled with 100 µM 5- chloro-2’-deoxyuridine (CldU; Sigma) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 2 mL of a 2.0×10^5 cells/mL solution were added to single cell culture dishes (EP0030700112, Sigma-Aldrich) and incubated overnight ...
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bioRxiv - Neuroscience 2023Quote: 5 dpf WT and scn1lab larvae were incubated in acridine orange (AO) (10 μM, Sigma Aldrich, CAS: 65-61-2) solution for 20 minutes followed by three 5 minute washes in embryo media ...
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bioRxiv - Neuroscience 2022Quote: ... and exposed to blocking solution containing 2% bovine serum albumin or 5% normal goat serum (all from Sigma Aldrich, USA). The fixed cells were incubated overnight at 4 °C with the following primary antibodies ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were separated by chromatography by injecting 2 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, EMD Millipore). The flow rate was set to 150 μl/min ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were separated by chromatography by injecting 2 μl of sample on a SeQuant ZIC-pHILIC Polymeric column (2.1 × 150 mm 5 μM, Millipore Sigma). Flow rate was set to 150 μl min ...
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bioRxiv - Cancer Biology 2024Quote: ... Dried samples were resuspended in 25 μL water and 2 μL was injected into a ZIC-pHILIC 150 x 2.1 mm (5 um particle size) column (EMD Millipore). operated on Vanquish™ Flex UHPLC Systems (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... media was changed once per week with the addition of 1 μM 5-Fluoro-2′-deoxyuridine (Cat.#F0503, Sigma-Aldrich) and 1 μM Uridine (Cat.#U3003 ...
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bioRxiv - Neuroscience 2023Quote: ... gilal cell growth and proliferation was inhibited by treating the cell cultures with 50μM 5-fluoro-2-deoxyuridine (Sigma-Aldrich). Mature cultures were used at DIV 11-12 for experiments and contained more than 95% neurons.
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bioRxiv - Neuroscience 2023Quote: ... animals were anesthetized with isoflurane (5% induction, 1-1.5% maintenance) and administered a subcutaneous dose of chlorprothixene (2 mg/kg, Sigma-Aldrich). During the mapping session ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized in 0.3% PBS-Triton X-100 for 40 minutes and blocked in PBS/0.1% Triton X-100 containing 2% BSA and 5% donkey serum (#D9663, Sigma-Aldrich). Primary antibodies were incubated overnight at 4°C in the blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Cell Biology 2023Quote: Mice were injected intraperitoneally three times daily for five consecutive days with 5-bromo-2’-deoxyuridine (BrdU, Sigma B9285-1G) diluted to 30 mg/kg in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were first incubated in 60% isopropanol for 5 minutes followed by staining with 2% Oil Red O (Cat# 00625, Sigma) in 60% isopropanol for 10 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... samples were blocked and permeabilized with 2% BSA and 0.1% Triton X-100 (#9036-19-5, Sigma-Aldrich now Merck) in PBS for 30 minutes at RT ...
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bioRxiv - Biochemistry 2022Quote: ... The cysteine bonds were reduced with a final concentration of 5 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma, 646547) for 60 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... Around 3 g of cells were suspended 1:5 in 50 mM Mops/KOH pH 7.0 supplemented with 2 mM dithiothreitol (DTT, Sigma-Aldrich) and disrupted by sonication while in an ice bath using a SONOPULS GM200 (Bandelin ...
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bioRxiv - Biophysics 2022Quote: ... 10 μM of biotin-labeled tubulin and 3.5 μM of Alexa647-labelled microtubules tubulin were incubated with 2 mM of GTP and 12 mg/ml CPA (Sigma) for 20 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Plates were incubated at 37 degrees C in 5% CO2 for 7 days before fixing with 2% formaldehyde and staining with 0.1% crystal violet (Sigma-Aldrich).