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Citations for Millipore Sigma :
4551 - 4600 of 10000+ citations for Cyclohexyl 2 3 4 5 trifluorophenyl ethyl ketone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... 3051.2) for 5 min and blocked in PBS containing 3% bovine serum albumin and 1% normal goat serum (Sigma-Aldrich, Taufkirchen, Germany, A4503 & G0023) at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... for 1-3 hours at room temperature (RT) and then permeabilized with 0.1% (v/v) Triton X-100 (Sigma-Aldrich, Cat # 9036-19-5) for 1 hour before blocking with 3% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... mice were gavaged with 0.2 ml of Fluorescein Isothiocyanate-Dextran (FITC-Dextran; 3–5 kDa; cat. no. FD4; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) in PBS ...
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bioRxiv - Microbiology 2023Quote: Transductions were carried out at 3×105 cells/mL and the indicated MOIs under normal culture conditions supplemented with 5 µg/mL polybrene (Sigma-Aldrich, TR-1003-G). Culture medium was replaced ∼24 hours after transduction and antibiotic resistance selection was carried out using 1.2 µg/mL puromycin for 2 days ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were diluted in a 5:1 (5X) stimulation buffer containing 500 µM of 3-isobutyl-1-methylxanthine (IBMX) (Sigma Aldrich, Saint Louis, MO). To an HTRF 96-well low volume white plate (CisBio) ...
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bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical], 1.2 units/mL Dispase II [Sigma-Aldrich], in perfusion buffer) for ∼45 minutes at 37° C using a rotating holder ...
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bioRxiv - Cancer Biology 2021Quote: Cytotoxicity of USP7 inhibitor P5091 and P22077 was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay (Sigma-Aldrich, St. Louis, MO, USA). Cytotoxicity was assessed after 24 hrs and 48 hrs of inhibitor treatment in 96 well plates.
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bioRxiv - Bioengineering 2022Quote: The HEK-pAGDisplay-RBD stable cells grown to 80% confluency were gently detached by Accutase (1/2 solution in PBS, 3 min, Sigma Aldrich cat. A6964) and washed by PBS with 2 g/L BSA (PBSB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactions were performed in 6 μL volumes on 384-well plates in reaction buffer containing 3 μL of 2 x Kapa Probe Fast qPCR Master Mix (Sigma-Aldrich, Dorset, UK). All probes were supplied by Thermo Fisher Scientific (Massachusetts ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, purity > 98%) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: The following chemicals in high grade were obtained commercially or as a gift: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) (Sigma-Aldrich, St Louis, MO, USA); Raloxifene (Cipla Ltd. ...
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bioRxiv - Immunology 2020Quote: ... 0.2 ml saline (0.9%) solution containing 1 mg Al (OH)3 as an adjuvant and 20 µg BLG (Sigma, St. Louis, MO, USA). Control mice received 0.2 ml saline (0.9% p/v ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 200μl media including 10 μL of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide, 5mg/mL) (Sigma-Aldrich, U.S., Cat. No. M2272) per well for 4h at 37°C (Ayazoglu Demir et al. ...
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bioRxiv - Neuroscience 2024Quote: ... aCSF and 192IgG-SAP groups received intraperitoneal (ip) injections of WIN55,212-2 (0.5 mg/kg or 3 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) or vehicle solution (1:1:18 ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were permeabilized with 0.2% Triton X-100 (Nacalai Tesque, Kyoto, Japan) for 15 minutes and blocked with 3% bovine serum albumin (BSA: Sigma Aldrich, MO, USA) for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... a RRP17-HA-AID strain was transformed with plasmids carrying respective constructs and growth assessed upon Rrp17-HA-AID depletion on plates containing 2 mM auxin (3-indoleacetic acid) (Sigma-Aldrich, Cat# I2886). Genetic experiments were performed at least in two biological replicates.
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Neuroscience 2024Quote: ... were used to calculate the amount of standard (30–70 µg) of 1-O- Heptadecyl-2-Acetyl-sn-Glycero-3-Phosphocholine (17:0 PC; Sigma-Aldrich cat# 850360P) previously diluted in 10 mL of chloroform was added as an internal standard prior to extraction ...
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bioRxiv - Immunology 2024Quote: ... The liver was chopped into small pieces (2 to 3 mm) and crushed with a syringe plunger before the collagenase D (1mg/ml in complete RPMI; Sigma life science, #11088882001) digestion 10 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Then the supernatant was separated by size to a large (>3 kDa) and small (<3 kDa) fraction using Amicon ultrafiltration system with 3 kDa filter (Millipore, Ireland). The fraction then filtered again through a 0.22 µm (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... N-(3-Oxoocatnoyl)-L-HSL (>97%) (3-Oxo-C8) and N-(3-Oxododecanoyl)-L-HSL (>98%) (3-oxo-C12) were used as received from Sigma-Aldrich. N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95% ...
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bioRxiv - Neuroscience 2024Quote: ... antibodies were diluted in a 3% BSA in PBS and either 3% normal goat serum or 3% normal donkey serum (Millipore-Sigma) depending on the secondary antibody host species ...
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bioRxiv - Pathology 2022Quote: ... 2 mM 2-mercaptoethanol (Sigma). Activity was measured using the Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Cancer Biology 2022Quote: ... (2) 2% Alconox (SIGMA, 242985) (20 min sonication followed by 5X washes with MilliQ H2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM 2-mercaptoethanol (Sigma). Proteasome Activity Fluorometric Assay II kit (UBPBio ...
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bioRxiv - Genetics 2021Quote: The Extensor Digitorum Longus (EDL) muscle was injured by intramuscular injection of 50 µL of 5 µM cardiotoxin (CTX) (Sigma, 11061-96-4). Mice were treated with carprofen 20 minutes prior to CTX injection and were injected with CTX under anesthesia by isoflurane ...
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bioRxiv - Immunology 2020Quote: ... the Jurkat cells were transduced with TCR lentivirus at an estimated multiplicity of infection (MOI) of 5 with 1 μl of polybrene at a final concentration of 4 μg/ml (Sigma, St. Louis, MO). Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... NIH/3T3 cells were infected with the corresponding lentiviruses (1/5 – lentivirus/cell culture medium) in the presence of 4 μg/mL polybrene (Sigma-Aldrich, cat# 107689). 4 days post-infection ...
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bioRxiv - Immunology 2023Quote: Infective-stage promastigotes (metacyclics) were isolated from 4–5-day old stationary culture by density gradient separation by Ficoll (Sigma-Aldrich, Cat #F9378). Mice were infected with 105 L ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were blocked in 5% BSA diluted in PBS before being incubated overnight at 4°C with rabbit ɑ-MBNL1 antibody (1:1000, Millipore Sigma, ABE241) or mouse ɑ-RFP antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was extracted from groups of 4-5 aphids of the above-described strains using TRI Reagent® (T9424, Sigma-Aldrich, USA) and Direct-Zol RNA extraction Kit (R2052 ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were perfused first for 5 min with NaCl 0.9% and then for 15 min with 4% paraformaldehyde (PFA, Sigma-Aldrich, St. Louis, MO) in sodium phosphate buffer (PB 0.1 M ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions were incubated for 4 hours at 37°C and 5% CO2 in supplemented RPMI 1640 media supplemented with PMA (50ng/mL, Sigma Aldrich, P8139-1MG), ionomycin (750ng/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were incubated overnight at 4°C with the following primary antibodies diluted in 5% TBST: GluA1-C (Millipore, Cat# AB1504, 1:1000), GluA2-C (NeuroMab ...
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bioRxiv - Biochemistry 2024Quote: ... Ireland) for 5 min at 4 °C using a Centricon 50 kDa molecular weight cut off (MWCO) concentrator (Millipore: MA, USA, Lot R0KA67379). After the 5 min spin ...
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bioRxiv - Molecular Biology 2021Quote: ... 2-Deoxy-D-glucose (2-DG; 2 mM, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... 2 mM 2-Mercaptoethanol (Sigma-Aldrich; Cas: 60-24-2), and 4 mM 4-Nitrophenyl acetate (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each mouse was intraperitoneal injected with 3 mL of 3% thioglycolate (Sigma-Aldrich) on day 1 and sacrificed using isoflurane on Day 3 ...
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bioRxiv - Neuroscience 2020Quote: ... after approximately 3 days in culture (DIV 3) cytosine β-D-arabinofuranoside (Sigma) was added to the hippocampal cultures in a 50% growth media exchange at a final concentration of 2 µM ...
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bioRxiv - Genomics 2020Quote: ... The cells were bathed in 3-3’ diaminobenzidine tetrahydrochloride (DAB) solution (Sigma Aldrich) during photobleaching.
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bioRxiv - Immunology 2020Quote: ... containing 0.25% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) (Sigma, St. Louis, MO) for two minutes at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... Three additional small molecules were introduced on day 3 (CHIR99021 (Sigma, 3 mM), SU5402 (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... 3-phosphoglyceric phosphokinase and glyceraldehyde 3-phosphate dehydrogenase were obtained from Sigma-Aldrich.
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bioRxiv - Plant Biology 2023Quote: ... in the presence of different concentrations of 3-aminotriazol (3-AT) (Sigma-Aldrich). Quantitative interaction assays were performed in liquid medium by quantifying β-galactosidase activity as previously described (52).
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bioRxiv - Biophysics 2023Quote: ... and 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide (EDC) were purchased from Sigma Aldrich Corp ...
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bioRxiv - Cancer Biology 2023Quote: Butyrolactone-3 (MB-3) was purchased from Sigma-Aldrich (St Louis, MO, USA) and used at a 100µM concentration ...
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bioRxiv - Immunology 2023Quote: ... 3 mM sodium 3-hydroxibutyrate as GPR81 antagonist (54965-10G-F, Sigma-Aldrich) and 0.5 μM AR-C 141990 hydrochloride (5658 ...
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bioRxiv - Cell Biology 2022Quote: ... Treatment with 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich cat #H7904) and Shield-1 (Cheminpharma cat #CIP-S1-0005 ...
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bioRxiv - Neuroscience 2021Quote: ... 4-Hydroxytamoxifen (4-HOT) (H-7904, Sigma, St. Louis, MO), the active metabolite of tamoxifen ...