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Citations for Millipore Sigma :
4601 - 4650 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin/Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 minutes followed by 3 washes in 40mM Tris buffer (pH=7.2) and then 1:20 Giemsa solution (Sigma-Aldrich). The blood and bone marrow were then imaged on a Zeiss Axio Imager.A2 and Zen Blue software (version 3.1).
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bioRxiv - Microbiology 2023Quote: ... All intracellular metabolite samples were resuspended in 750μL deuterium oxide containing 1mM 3-(Trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS) (178837, Sigma Aldrich) and 50mM NaIO4 (769517 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then washed with lysis buffer containing 0.1% DDM and incubated with lysis buffer containing 1% DDM and 3×Flag peptide (100 µg/ml, Sigma #F4799) overnight for elution ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin, Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/ml ...
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bioRxiv - Evolutionary Biology 2023Quote: ... collected on a fine nylon mesh and transferred to a well of a 12-well cell culture plate containing 3 mL of 1% sodium dodecyl sulfate (SDS, Sigma) to facilitate egg dispersion ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... spores were sown directly on plates supplemented with Indole-3-acetic acid (IAA; Alfa aeser) or 1-Naphthaleneacetic Acid (NAA, Sigma). Size measurements were done when plants reached sexual maturity (±6/7 days) ...
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Cancer Biology 2024Quote: HSJD-DIPG007 neurospheres were collected by centrifugation at 800 rpm for 3 min and dissociated into single cells with 1 mL Accutase (Sigma) for 5-10 minutes with occasional pipetting ...
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bioRxiv - Neuroscience 2024Quote: ... 0.3% Triton X-100 (PBST)) for 10 minutes and blocked for 1 hour with 10% normal donkey serum (Merck Millipore), 1% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Neuroscience 2024Quote: ... and the samples were washed 3 times for 5 min in PBS and counter-stained with 1:1000 DAPI (D9542, Sigma) for 15 min ...
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bioRxiv - Genetics 2024Quote: ... Then cells were washed with cold staining buffer to terminate reaction and incubated with rabbit complement sera (1:3 dilution, Cat. no. S7764, Sigma) for 30 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Fully grown oocytes were collected 48 hours after injection and placed in M2 medium containing 200 μM 3-isobutyl-1-methyl-xanthine (IBMX, Sigma) at 37°C ...
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bioRxiv - Genetics 2024Quote: ... 29 Starved HAP1 cells were washed twice with DPBS supplemented with 1% v/v Phosphatase Inhibitor Cocktail 3 (Sigma #P0044) before trypsinization with trypLE (trypLE ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were washed with distilled water 3×5min before being counterstained with DAPI solution diluted in PBS (1:50,000, Sigma-Aldrich) for 5 min ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies were used in this study: rabbit anti-phospho-Histone-3 (1:1000; Sigma-Millipore, 05-570) and rat anti-Miranda (1:500 ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies were used in this study: rabbit anti-phospho-Histone-3 (1:1000; Sigma-Millipore, 05-570) and rat anti-Miranda (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... 50 µl of the crude extracts were kept for input and 350 µl of the crude extracts were mixed with 30 µl of anti-Myc antibodies (1:5,000 dilution, MBL: M192-3) bound to Protein G Sepharose beads (Sigma- Aldrich) for overnight at 4°C on a nutator (BenchMark) ...
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bioRxiv - Microbiology 2024Quote: ... The samples were diluted 4-fold with 50 mM Tris-HCl pH 8 and digested by 0.4 μg Lys-C (Wako, Japan) treatment for 3 hours at room temperature and 1 μg of trypsin (Sigma, MO) overnight at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and their lungs were minced with scissors and digested in 3 ml of RPMI-1640 with 5% heat-inactivated FBS containing 1 mg/ml collagenase D (Sigma) and 30 µg/ml DNAseI (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA was injected into the fourth ventricle at a final concentration of 1-3 µg/µl in addition to trace amounts of fast-green dye (Sigma). Three 50ms square waveform electrical pulses at 5V (E2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmids were transfected in a 3:1 PEI:DNA ratio into 90% confluent HEK293 cells in a 10cm dish (PEI=polyethylenimine, Sigma 408727). On the day of transfection ...
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bioRxiv - Genomics 2024Quote: ... we treated 3 dpf larvae twice for 1 hr each with 300 μM neomycin sulfate (Sigma-Aldrich, St. Louis, USA) with 6 hrs of recovery between treatments ...
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bioRxiv - Biochemistry 2024Quote: ... then we incubated it for 1 minute with 10 µl of 0.1% (v/v) 3-aminopropyl-triethoxysilane (APTES; Sigma Aldrich) in Milli-Q water ...
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bioRxiv - Developmental Biology 2024Quote: EHMT-dTAG-MERVL cells treated with DMSO or dTAG for 24hr were washed and fixed in 4% formaldehyde for 10mins, blocked in blocking buffer (PBS-/- with 1% BSA, 0.1% Triton-X-100, and 3% donkey serum (Sigma D9663) and stained with ZSCAN4 antibody (AB4340 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-adducin phospho S355 antibody (Abmart 20592-1NB-3/C63-S, 1:100, for WB and double transgenics) anti-FLAG antibody (M2, F3165, Sigma-Aldrich 1:100). Myosin Light Chain 2 antibody (ptglab ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was exchanged with 50 μL of fresh medium with 1 mM 3-isobutyl-1-methylxanthine (IBMX) (Sigma-Aldrich, Cat# I7018-100MG), and cells were incubated at 37°C for an additional 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168 ...
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bioRxiv - Neuroscience 2022Quote: ... CAS# 83-34-1), indole (98%, CAS# 120-72- 9), and 3-ethylindole (97%, CAS# 1484-19-1) were provided by Sigma-Aldrich (Milwaukee, WI), ACROS Organics (Geel ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Biochemistry 2023Quote: ... and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM) chloride (Sigma-Aldrich) in buffer C for 1 h at 37°C and 500 rpm using a ThermoMixer (Eppendorf) ...
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bioRxiv - Neuroscience 2021Quote: Protein lysates were extracted from the hippocampi or cortices of adult mice and dissected in ice-cold PBS containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and Mini-Complete Protease Inhibitor Cocktail (Roche Diagnostics ...
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bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (2 months old) were transduced with 250 µl of Auto-hLAG3 LV and 3 µg/ml of polybrene (Sigma-Aldrich #TR-1003-G) per well of a 6 well plate ...
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bioRxiv - Bioengineering 2020Quote: ... Aortas were then washed in 78% methanol for 5 minutes under gentle movement for a total for 3 times before incubating in a 2% ORO solution (Sigma-Aldrich, St Louis, MO) for 60 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... The larvae were then optically cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min of incubation ...
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bioRxiv - Neuroscience 2020Quote: Protein lysates were extracted from the hippocampi or cortices of adult mice and dissected in ice-cold PBS containing Phosphatase Inhibitor Cocktails 2 and 3 (Sigma-Aldrich, St. Louis, MO) and Mini-Complete Protease Inhibitor Cocktail (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with lysis buffer (RIPA buffer, 1x Roche Complete Protease Inhibitor cocktail, 1X Sigma Phosphatase Inhibitor Cocktail 2, and 1X Sigma Phosphatase Inhibitor Cocktail 3), and incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: Alveolar organoids were removed from Matrigel by mechanical dissociation with a cut pipette tip or retrieved from HA hydrogels with 2% hyaluronidase (Sigma Catalog #37326-33-3) for 30 minutes at 37°C followed by mechanical dissociation with a pipette tip ...
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bioRxiv - Physiology 2023Quote: 25-30 mg of frozen powdered liver tissue was used to extract liver lipids as described previously 34 and reconstituted in a tert-butanol-Triton X solution (3:2) and liver triglycerides and cholesterol levels were measured via a commercially available kit (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2023Quote: ... and 3 μg/mL + 7 μg/mL FRD+U (5-Fluoro-2′-deoxyuridine, Sigma-Aldrich, Cat# F0503 and Uridine, Sigma-Aldrich, Cat# U3003-5G). 120 µL of cell suspension was carefully plated onto each coverslip and allowed to adhere for 2 hours at 37°C and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... an isolated infrarenal aortic segment of C57BL/6J mice was perfused with sterile isotonic saline containing type I PPE (2-3 U/ml, E1250, Sigma-Aldrich, Burlington, MA, USA) for 5 min under pressure (120 mmHg ...
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bioRxiv - Physiology 2023Quote: ... In order to substitute cholesterol in the patch with cholest-4-en-3-one (cholestenone) the bath solution was replaced with 2 mM MBCD solution saturated with cholest-4-en-3-one (Millipore-Sigma, St. Louis, MO).
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bioRxiv - Microbiology 2023Quote: ... freshly drawn whole blood was mixed with an equal volume of a solution containing 3% dextran (Sigma-Aldrich, ACS Cas.# 9004-54-2, Denmark) and 1.8% sodium chloride (Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 3 (10 mg/kg) intraperitoneally (i.p.) 30 min prior to injection of zymosan (Sigma-Aldrich; 2 mg/ml in saline, 0.5 ml, i.p.). Mice were sacrificed (by CO2 inhalation ...
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bioRxiv - Cell Biology 2024Quote: ... Phase separation of lipids from the serum was performed using an organic phase of diisopropyl ether: n-butanol (3:2; Sigma Aldrich, 296856, B7906, respectively). Organic and aqueous phases were allowed to mix at room temperature with continuous stirring in the dark for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... a 5 µL drop of the formulation was placed on a glass slide that was silanized by liquid phase deposition in a solution of 2% 3-(trimethoxysilyl)propyl methacrylate (cat. #M6514, Sigma-Aldrich, Oakville, Ontario, Canada) prepared in toluene (cat ...
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bioRxiv - Synthetic Biology 2022Quote: ... then mixed with 1 mL of the reactive dye 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF) (Sigma Aldrich), previously dissolved in DMSO (20 mg/mL) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.1% 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl)_(NBD-PE) in a 4:1 mixture of silicone oil (Sigma-Aldrich) and mineral oil (Sigma-Aldrich ...