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Citations for Millipore Sigma :
4551 - 4600 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the medium was exchanged for differentiation medium I [supplemented DMEM containing 0.5 mM 3-isobutyl-1-methylxanthine (IBMX, Sigma Aldrich), 0.25 μM dexamethasone (Dex ...
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bioRxiv - Immunology 2021Quote: ... Cy5- or Cy7-PMPC25-PDPA68 copolymer was dissolved together with the PMPC25-PDPA68 in an organic solution of 3:1 (v/v) methanol:tetrahydrofuran (Sigma-Aldrich) for a final copolymer concentration of 20 mg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... and transcription factor SREBP1a (aa 1-50) were generated by inserting the corresponding cDNA fragments into pGEX4T-3 vector (Sigma). V5-tagged full-length and N-terminal truncation (Δ302 ...
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bioRxiv - Cell Biology 2020Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Developmental Biology 2021Quote: ... The filtrate was centrifuged for 3 min at 300 x g and resuspended in 1 mL of 50 mg/mL BSA (Cat.No. A8806; Sigma-Aldrich) in phosphate buffered saline (PBS) ...
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bioRxiv - Developmental Biology 2020Quote: ... plates of 3 mL ASW+BSA were supplemented with 1:1000 dilution of U0126 stock solutions (Sigma Cat. #662005-1MG) dissolved in DMSO to produce the indicated concentrations of U0126 ...
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bioRxiv - Cell Biology 2020Quote: ... were mixed in a 1:3 molar ratio and incubated overnight at room temperature with calf intestinal alkaline phosphatase (Sigma). After 12-18h ...
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bioRxiv - Biophysics 2021Quote: ... DNA and RNA samples used for NMR measurements were buffer exchanged into the desired NMR buffer with final concentration ~1 mM using Amicon Ultra-0.5/15 centrifugal concentrators (3-kDa cutoff, Millipore Sigma). The samples used for optical melting experiments were prepared by diluting NMR samples to ~3 μM using buffer ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were frozen at a concentration of 1-3×107 cells/ml in 10% DMSO (Sigma-Aldrich, St. Louis, MO)/90% FCS (Atlanta Biologicals ...
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bioRxiv - Physiology 2023Quote: ... Following overnight incubation at 4°C with primary antibodies against CARNS1 (rabbit polyclonal, 1:1000 in 3% milk/TBS-T, HPA038569, Sigma), membranes were washed (3 × 5 min) ...
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bioRxiv - Genomics 2022Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody in 3% BSA/TBS-T (monoclonal mouse anti-FLAG, 1:1,000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Physiology 2023Quote: ... Splenocytes were incubated for 1 hour at 37°C in 96-well plates in RPM11640 medium (3 ml) (Sigma-Aldrich) with or without EPO (35U/well ...
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bioRxiv - Developmental Biology 2023Quote: ... Product number E4250) (1 mg/ml) to contract pigment cells alongside 0.01% Tricaine (Ethyl 3-aminobenzoate methanesulfonate, Sigma-Aldrich (Merck) Product number E10521 ...
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bioRxiv - Microbiology 2023Quote: ... A 4 µL sample of the stock suspension of latex beads (containing about 3×1010 mL-1 of 0.3 µm diameter beads, Sigma-Aldrich) was added to each protein and incubated for 2 h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... at 1:1000 dilution at room temperature for 1 hour followed by washes x 3 then staining with goat anti-rabbit HRP-conjugated antibody (Catalog #204903, Sigma) at 1:5,000 dilution at room temperature for 1 hour ...
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Transport of metformin metabolites by guanidinium exporters of the Small Multidrug Resistance familybioRxiv - Biophysics 2023Quote: ... 100 mM KCl and pre-loaded with 0.4 mM substrate (Gdm+ or guanylurea) and 1mM pyranine (trisodium 8-hydroxypyrene-1, 3, 6-trisulfonate; Sigma-Aldrich) using three freeze/thaw cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... Floxing of the transgenic 3’ kappa enhancer was achieved by treating tumor bearing mice with 1 mg tamoxifen (Sigma-Aldrich) in corn oil given by daily i.p ...
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bioRxiv - Bioengineering 2023Quote: ... and ligation using T4 DNA ligase (NEB at 16 °C for 16 hours with a 1:3 molar ratio of insert to vector. Ligations were dialyzed on Millipore VSWP 0.025 μm membrane filters for 90 minutes before electroporating (2mm cuvettes at 2.4kV ...
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bioRxiv - Cell Biology 2023Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Developmental Biology 2024Quote: ... Auxin treatment was performed by transferring worms to bacteria-seeded NGM plates containing 1 mM auxin (indole-3-acetic acid, Sigma). The preparation of auxin stock solution (400 mM in ethanol ...
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bioRxiv - Neuroscience 2022Quote: ... Washed cells were first incubated for 1 h at room temperature (RT) with blocking solution (3% bovine serum albumin [BSA, Sigma, A7906] and 0.5% Triton X-100 [Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... and a 0.2 nM αV imager strand buffer solution (5-GGAGGA-3’-Atto643, Eurofins) were made using 1× PCA (Sigma-Aldrich), 1× PCD (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... Doxycycline-inducible expression was tested in each cell line using 3 different concentrations (0.1-1 μg/mL) of doxycycline hyclate (Sigma-Aldrich) and a no-doxycycline control to evaluate Orf3a expression leak ...
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bioRxiv - Physiology 2024Quote: ... cells were incubated with blocking buffer (PBS containing 3 % milk and 0.1 % Triton X-100) and primary antibodies against ACTN2 (1:800, Sigma, A7811) and dTyr-tub (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells with stable integration of the hairpin were selected with 1 μg/ml puromycin and also sorted by GFP flow cytometry after 3 days of 100 ng/ml doxycycline (Sigma) treatment.
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bioRxiv - Immunology 2024Quote: ... AHR was assessed by administering incremental doses of nebulizing methacholine (0, 1, 3, 9, 27, 81 mg/mL; Sigma-Aldrich) and measuring resistance every 30 seconds ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting ribosome solution (∼1 mL) was further concentrated using 0.5 mL Amicon Ultra Filter unit 3 KDa MWCO (Sigma-Aldrich) by centrifugation at 14,000 RCF and 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... homogenized in cytosol lysis buffer (10 mM Hepes, 40 mM KCl, 3 mM MgCl2, 5% glycerol, 0.2% NP40, 1 mM DTT, cOmplete protease inhibitors, Sigma-Aldrich) and incubated for 30 min at 4°C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... stage were collected 48 hours after injection and released into M2 medium containing 200 µM 3-isobutyl-1-methylxanthine (IBMX, Sigma) at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: Worms of the indicated genotype were selected as L4s or young adults (24h post L4 stage) and picked onto NGM plates with and without 1 mM indole-3-acetic acid (auxin, Sigma). Exposure to auxin was carried out for the indicated times ...
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bioRxiv - Synthetic Biology 2023Quote: ... or M9 minimal media supplemented with fructose or glucose (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl (Sigma–Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Developmental Biology 2023Quote: ... for 5 minutes followed by 3 washes in 40mM Tris buffer (pH=7.2) and then 1:20 Giemsa solution (Sigma-Aldrich). The blood and bone marrow were then imaged on a Zeiss Axio Imager.A2 and Zen Blue software (version 3.1).
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bioRxiv - Microbiology 2023Quote: ... All intracellular metabolite samples were resuspended in 750μL deuterium oxide containing 1mM 3-(Trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS) (178837, Sigma Aldrich) and 50mM NaIO4 (769517 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then washed with lysis buffer containing 0.1% DDM and incubated with lysis buffer containing 1% DDM and 3×Flag peptide (100 µg/ml, Sigma #F4799) overnight for elution ...
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bioRxiv - Cell Biology 2023Quote: ... when embryoid bodies were formed) half of the medium was replaced with differentiation medium supplemented with 3 μM IWR-1-Endo (Sigma), 1 μM Dorsomorphin (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were diluted 1 in 100 in a 3 mL volume of YPD and compounds were added to final concentrations of 1 µg/mL for Rhodamine 6G (Sigma), or 1 µM for FLC-Cy5 ...
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bioRxiv - Immunology 2024Quote: ... Fetuin-coated plates were then washed 3 times with PBS-T and incubated for 1 h with a solution of PNA-HRP (cat. # L6135-1MG, Sigma) at 5 μg/ml in conjugate diluent (MES pH 6.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cells were washed 3 times with 1×PBS and then permeabilized with 0.125% TritonX-100 (Sigma Aldrich, no. 93443) in 1×PBS for 2 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin, Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin/Streptomycin, LIF and 2i (3 μM Gsk3 inhibitor CT-99021, 1 μM MEK inhibitor PD0325901) and Vitamin C (Sigma) at a final concentration of 100 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:3 with a balanced salt solution (1X Dulbecco′s PBS (DPBS, Cat No. TMS-012-A Sigma Aldrich) fortified with 2% FBS (Cat No ...
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bioRxiv - Immunology 2023Quote: ... cells were pretreated with LPS for 3 h and then treated with LP for 1 h in a Ca2+-free Krebs-Ringer bicarbonate (KRB) buffer (Sigma) to eliminate the effect of extracellular Ca2+ influx into ER (Xu et al. ...
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bioRxiv - Bioengineering 2023Quote: ... diametrically-halved constructs (n=3) were stained over the exposed cross section with a speckled pattern of 1% hematoxylin (Sigma) via airbrush ...
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bioRxiv - Biochemistry 2024Quote: ... 0.01% C12E9) and elution was performed using 3 column volumes of buffer C supplemented with 1 mg/mL d-desthiobiotin (Sigma). The elution fraction was concentrated to 0.5 mg/mL using 100-kDa cut-off filters (Millipore ...
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bioRxiv - Evolutionary Biology 2023Quote: ... collected on a fine nylon mesh and transferred to a well of a 12-well cell culture plate containing 3 mL of 1% sodium dodecyl sulfate (SDS, Sigma) to facilitate egg dispersion ...
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bioRxiv - Cell Biology 2022Quote: ... washed in ice-cold PBS and lysed in room temperature NP40 lysis buffer (50 mM Tris pH 7.4, 140 mM NaCl, 3 Na3VO4, 1% v/v IGEPAL CA-360 (Sigma, #I8896), Phosphatase Inhibitor cocktail (PhosStop ...
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bioRxiv - Cell Biology 2022Quote: ... The selected elution fractions (1-3 of 500 μL each) were pooled and subsequently concentrated using 100-kDa Amicon centrifugal filter units (Merck Millipore). The concentrated samples were subjected to several washing steps with PBS to obtain a highly pure EV population ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed (3 × 5 min. in PBS at RT) before staining with DAPI (1:1000; Sigma Aldrich, D9542) and AlexaFluor-488 Phalloidin (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... round #1 German glass coverslips (Electron Microscopy Services) were coated with 0.5% v/v (3-Aminopropyl)triethoxysilane (APTES, Sigma, 440140), 99.2% v/v ethanol ...
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bioRxiv - Developmental Biology 2022Quote: ... Prior to start of imaging 6.5 ml culture medium with 1 μg/ml 17alpha,20beta-Dihydroxy-4-pregnen-3-one (DHP, diluted in 100% Ethanol, Sigma) containing drug/Lysotracker was added on top of the solidified LMP agarose ...