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Citations for Millipore Sigma :
4501 - 4550 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... a radiolabelled 3′-biotinylated 30-mer DNA substrate (Supplementary Figure 2A, from Sigma Aldrich) was bound to a Streptavidin monomer (Promega ...
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bioRxiv - Systems Biology 2024Quote: ... and beads were subsequently washed 3 times with 80% HPLC grade-ethanol (Sigma Aldrich) and then resuspended with 175 uL of Trypsin/Lys-C (1 μg ...
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bioRxiv - Biophysics 2024Quote: ... we assayed 3 different FBS batches using the Glucose Assay Kit (Sigma Aldrich, GAGO20), one batch that was heat inactivated (STEMCELL Technologies lot 2030348 ...
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bioRxiv - Cell Biology 2024Quote: ... Cell suspension was further mixed with fibrinogen (3 mg/mL at final concentration, Sigma) on ice ...
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bioRxiv - Bioengineering 2024Quote: ... Mice were administered daily doses of PP (CAS#94-13-3, Sigma Aldrich, USA) or vehicle control (corn oil ...
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bioRxiv - Cell Biology 2024Quote: ... HeLa cells (1.0 × 105 cells) were plated with 3 mM thymidine (T9250, Sigma-Aldrich) for 18 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Fixed cells were blocked in 3% (w/v) bovine serum albumin (BSA) (Sigma-Aldrich) in PBS and incubated with corresponding primary and secondary antibodies diluted in 3% BSA in PBS for 1-2 h RT ...
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bioRxiv - Cell Biology 2024Quote: ... samples were stained for 3 min with a nuclear fast red solution (Sigma-Aldrich). Slides were dehydrated and mounted in Ultrakitt mounting medium (VWR) ...
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bioRxiv - Cell Biology 2024Quote: ... Naïve medium was made of N2B27 with 3 µM of GSK3β inhibitor (Sigma SML1046), 1 µM of MEK inhibitor (Sigma PZ0162) ...
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bioRxiv - Biochemistry 2022Quote: Phagosomal proteins from RAW264.7 cells were extracted in 1% sodium 3-[(2-methyl-2-undecyl-1,3-dioxolan-4-yl)methoxy]-1-propanesulfonate (commercially available as RapiGest, Waters) in 50 mM Tris (Sigma) pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed 3 times with PBS before adding fresh post-infection media containing 1% microcrystalline cellulose (Avicel, Sigma Aldrich). Cells were further incubated at 37 °C for 24 h ...
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bioRxiv - Biophysics 2020Quote: ... the following target sequences were used: 5’-GCTTCAGGATTCAATGCCATGG-’3 (#1) using the all-in-one CRISPR/Cas9 plasmid (Sigma-Aldrich) followed by cell-sorting for GFP expression to generate single cell clones with disrupted ANXA4 reading frame.
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bioRxiv - Cancer Biology 2021Quote: ... the plates were cultured for 2-3 weeks in the incubator at 37°C with 5% CO2 before being stained with 1% crystal violet reagent (Sigma) and counted.
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bioRxiv - Microbiology 2020Quote: ... Hereafter the samples were digested for 3 h – overnight in digestion buffer (50 mM Tris pH 8.0, 1 mM EDTA (Sigma, ED2P), 0.5 % Triton X-100 (Thermo Fisher ...
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bioRxiv - Cell Biology 2020Quote: ... These slides were rehydrated in 1x PBS at room temperature for 15 min followed by blocking for 1 hour in 1x PBS containing 3% BSA (Bovine serum albumin, Sigma). The slides were then incubated for 1 hour at room temperature with 1xPBS+3% BSA with respective antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... and Ypt1-7xHis were mixed in a 1:3 molar ratio and incubated overnight at 4°C with calf intestinal alkaline phosphatase (Sigma) to hydrolyze nucleotide ...
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bioRxiv - Cell Biology 2020Quote: ... GV oocytes were cultured in M2 medium supplemented with 0.2 mM of the phosphodiesterase inhibitor 3-Isobutyl-1- methylxanthine (IBMX, Sigma-Aldrich) at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Staining with Cyanine dye 2 (Cy2)-conjugated streptavividin (Jackson Immuno Research, 1:400 in PBS, with 3% bovine serum albumin, Jackson Immuno Research and 0.05% sodium azide, Sigma-Aldrich) for 60 min was used to identify biocytin-filled cells ...
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bioRxiv - Molecular Biology 2021Quote: ... 18 μL (1:3 ratio of DNA to transfection reagent) of X-tremeGENE™ 9 DNA transfection reagent (MilliPore Sigma). The mixture was incubated for 30 mins at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were grown in M9 minimal media supplemented with glucose containing M9 salts (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl) (Sigma-Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Bioengineering 2020Quote: ... the chips were silanized by immediately adding 0.5 ml of a fresh 1% aqueous solution of 3- (Ethoxydimethylsilyl) propylamine (Sigma, 588857) into the culturing chamber of the chip and incubated for 15 min at RT before rinsing with twice with 1 ml dH2O water ...
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bioRxiv - Microbiology 2020Quote: ... parahaemolyticus cells incubated in ASW microcosms at 4°C for 100 days were re-suspended in 50 mM potassium phosphate buffer (pH 7) containing 1 g of 3-mm-sized glass bead (Sigma), vortexed for 25 min ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)11 (Vero passage 3) was kindly provided by CDC and propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... The animals were then subjected to increasing doses of methacholine (0.1, 0.3, 1, 3, 10, 30mg/mL) (a broncho-constrictor; acetyl-β methacholine chloride; Sigma-Aldrich) delivered by an ultrasonic nebuliser ...
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bioRxiv - Genomics 2021Quote: ... Cells were pelleted at 600 g for 3 min and resuspended in 400 μl Wash Buffer 1 (wash buffer with 0.05 % saponin (Sigma Aldrich), protease inhibitor cocktail (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 isolate nCoV-WA1-2020 (MN985325.1)49 (Vero passage 3) was kindly provided by the CDC and propagated once in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Bioengineering 2021Quote: ... the precursor solution for the Zn0.4Fe2.6O4 nanoparticles was prepared at a con-centration of 0.2 molZn+Fe / L in a 3:1 volume ratio of xylene and acetonitrile (both Sigma-Aldrich). Iron acetylacetonate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and aphidicolin (7.5µM) before cells were treated for 3 hours with nutrients (1 µM insulin plus 0.8 mM L-leucine [Sigma Aldrich catalog numbers I5500 and L8000 ...
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bioRxiv - Cell Biology 2021Quote: ... at 37°C in the presence of Zombie Red (1/1000) according to the manufacturer’s procedure (Ozyme) then trypsinized and fixed in PBS with formalin 3% (Sigma Aldrich). For F-actin quantification ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were transfected using X-tremeGENE 9 (ratio 3:1 μl/μg DNA) according to the manufacturer’s protocol (Sigma-Aldrich). DNA encoding PAC constructs and SthK-EGFP was used at a 3:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... Immunoprecipitation was performed at 4°C overnight with nutation in 1 ml chromatin aliquots with 3 ul of anti-FLAG M2 antibody (F3165, Sigma) and 20 ul of Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... media (ratio 1:3) for 30-45 min until the digestion was stopped by the addition of FBS (Sigma, Germany) at a 10 % v/v ratio ...
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bioRxiv - Neuroscience 2020Quote: ... animals were mounted by placing them in 3% agarose pads on glass slides and immobilized with 1 mM tetramisole hydrochloride (Sigma). Imaging was performed using Confocal Laser Scanning Confocal microscope (Olympus 1X61 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then solubilized by mixing 3:1 lysate to Li-Cor 4x loading buffer (928-40004) plus 10% Beta-Mercaptoethanol (Sigma). Samples were denatured by boiling for 5 min followed by cooling on ice for 2 min ...
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was performed on 12% SDS-PAGE gels using 1-hour semi-dry transfer with 3% bovine serum albumin (BSA, Sigma) in TBS + 0.5% Tween-20 as a blocking agent.
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 1 × 105 Cas9 TNG MKOS MEFs were transduced with either a non-targeting control sgRNA or Zfp266 sgRNA lentivirus at an MOI of 3 with 8 µg ml−1 polybrene (Merck-Millipore) for 4 hours ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain MG1655-seeded NGM plates in the presence or absence of 1 mM auxin (IAA, indole-3-acetic acid, Sigma).
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Tg[elavl3:Gal4;10xUAS:jRGECO1b] at 3 dpf were paralyzed by a 5-min bath application of 1 mg/ml a-bungarotoxin (Sigma, 203980). Larvae were mounted on their side in a field stimulation chamber (Warner ...
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bioRxiv - Microbiology 2020Quote: ... The plasma-treated coverslips were then transferred into 150 mL of 1% (v/v) (3-Aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich) in toluene (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (sodium salt) 18:1 PS (DOPS) was purchased from Sigma-Aldrich. N-((6-biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanoamine ...
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bioRxiv - Molecular Biology 2020Quote: 25 mm round cover glass (Fisher Brand, #12-545-102 25CIR-1) was coated with 3-Triethoxysilypropylamine APES (Millipore, #A3648) following manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: In vitro treatments were carried out with different compounds: 1 μM MG-132 24 hours or 3 hours (Merck Millipore), 20 μM Chloroquine (CQ ...
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... The cloning was performed according to the manufacturer’s protocol using the T7Select® 415-1 Cloning Kit (Millipore, 70015-3) with 1 μL of annealed oligonucleotides.
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bioRxiv - Genetics 2020Quote: ... 150mM NaCl) including 3% milk powder for 1 hour and incubated overnight with anti-FLAG antibody (Sigma Aldrich, cat. # F1804) in TBS plus 3% milk ...
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bioRxiv - Synthetic Biology 2021Quote: ... and M9 minimal media supplemented with glucose (6.78 g/L Na2HPO4, 3 g/L KH2PO4, 1 g/L NH4Cl, 0.5 g/L NaCl (Sigma-Aldrich, M6030), 0.34 g/L thiamine hydrochloride (Sigma T4625) ...
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bioRxiv - Microbiology 2021Quote: ... mobile phase and stained using ethanol: sulfuric acid (70:3 v/v) solution containing 1% (w/v) orcinol monohydrate (Sigma). HPAEC-PAD samples were diluted to 50 μM and resolved on a Dionex PA20 column in a mobile phase of 30 mM NaOH and gradient of 10 mM – 120 mM sodium acetate over 40 min at a flow rate of 0.5 mL min-1 ...