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Citations for Millipore Sigma :
4401 - 4450 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... Protein was visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, #WBKLS0500) and imaged on a ChemiDoc (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins bound to anti-Flag M2 affinity beads (Sigma-Aldrich) were recovered from the supernatants and washed by Flag wash buffer (50 mM NaPi (pH 7.5) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto Immobilon-P PVDF membranes (Millipore, Darmstadt, Germany) in 1× Tris Glycine with 20% ethanol ...
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bioRxiv - Cell Biology 2024Quote: ... and the protein concentration was determined with Bradford reagent (Sigma-Aldrich). Lysates were subsequently denatured in Laemmli sample buffer for 5 minutes at 95°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to a 0.45 µm PVDF membrane (Millipore). Primary antibodies were used at the following dilutions in 5% Blotting-Grade Blocker (BioRad) ...
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bioRxiv - Developmental Biology 2023Quote: ... protein lysates were prepared in 1× RIPA buffer (Millipore, #20-188) from Drosophila head tissue expressing UAS-FLAG-Dx ...
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bioRxiv - Genetics 2024Quote: ... the proteins were transferred onto a nylon membrane (Immobilon-P, Millipore) and incubated with primary antibodies in a blocking buffer (1ξ PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pellets were resuspended in BugBuster® protein extraction reagent (Millipore). Protein from the lysed cell suspension was then extracted by chloroform/methanol precipitation ...
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bioRxiv - Immunology 2023Quote: ... FLAG-tagged proteins were immunoprecipitated with anti-FLAG M2-agarose (Sigma), following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined by a Bradford assay (Sigma Aldrich; B6916). Proteins were denatured in NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Biochemistry 2023Quote: The lysate was pre-cleared with ∼20µL Protein G agarose (Sigma) per 1mL of lysate at 4°C for 30min ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-pro-surfactant protein C (SFTPC, 1:1000, AB3786, Millipore), goat anti-SOX9 (SOX9 ...
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bioRxiv - Immunology 2023Quote: ... injection of 30 μg of OVA protein (Grade V; Sigma-Aldrich) adsorbed to 2 mg of aluminum hydroxide (alum ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were blotted onto Immobilon-P PVDF membrane (0.2 µm, Millipore) using a Trans-Blot Semi-Dry transfer Cell (BioRad ...
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bioRxiv - Systems Biology 2023Quote: ... total protein amounts were assayed using Fast Green FCF (Sigma, #F7252) staining solution47 and imaged using LICOR Odyssey 700 nm ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins in gel was visualized with sliver staining kit from Sigma. The gels over 100 KDa (SATB2 molecular weight ...
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bioRxiv - Microbiology 2023Quote: ... lysates were incubated with PureProteome protein A/G magnetic beads (Sigma) for 1-2 h ...
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bioRxiv - Microbiology 2023Quote: ... the protein was transferred onto a polyvinylidene difluoride (PVDF) membrane (Millipore), and proteins were detected by probing with specific antibodies (Table S7 ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated with PureProteome protein A/G magnetic beads (Sigma) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... along with several dilutions of a SOD1 protein standard (Sigma, S9697) were separated on native 12% Tris-Glycine acrylamide gels (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were transferred to an Immobilon polyvinylidene difluoride (PVDF) membrane (Millipore) using a wet transfer Mini Trans-Blot Cell (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... filtered through a 0.45-µm low-protein-binding Durapore filter (Millipore), and stored at −80°C until use ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were concentrated with a 30 kDa cutoff Amicon concentrator (Millipore), and gel-filtered on a Superose 6 10/30 (GE Healthcare ...
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bioRxiv - Developmental Biology 2023Quote: ... strep tag was removed from the eluted proteins by thrombin (Sigma) digestion overnight at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred to the polyvinylidene fluoride (PVDF) membrane (Millipore, USA) which was subsequently fixed with 8% acetic acid for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were visualized using Immobilon Forte Western HRP Substrate (Millipore, WBLUF0500).
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bioRxiv - Biochemistry 2023Quote: ... protein tags were removed by tobaccoetchvirus (TEV) protease (#T4455, Sigma-Aldrich) (1:25 w/w ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: ... Pure protein solutions were concentrated by centrifugation in Centricon tubes (Millipore).
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bioRxiv - Molecular Biology 2023Quote: Total proteins were extracted with RIPA buffer (Sigma Aldrich, Missouri, USA) supplemented with protease and phosphatase inhibitor cocktail then dosage was carried out with BCA protein quantification kit (Life Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... and then the proteins were transferred to the PVDF membranes (Millipore). After being blocked by the 3% BSA (Sigma ...
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bioRxiv - Physiology 2023Quote: ... Protein concentrations were determined using a bicinchoninic acid assay (Sigma, #BCA1), and the resulting absorbance was read at 562 nm ...
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bioRxiv - Neuroscience 2023Quote: ... The protein coated membrane was blocked with 5% normal serum (Sigma) and 5% skim milk (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... and monoclonal anti-glial fibrillary acidic protein (GFAP) IgG (G3893, Sigma) and secondary antibodies Alexa Fluor 647 and Alexa Fluor 488 were applied to label neurons and astrocytes ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was quantified using a BCA assay or by Millipore Direct Detect ...
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bioRxiv - Neuroscience 2023Quote: ... and amyloid precursor protein (APP) (A8717, Sigma-Aldrich, St. Louis, MO) or monoclonal antibody against β-actin (A2228 ...
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bioRxiv - Genetics 2023Quote: ... Proteins were transferred to Immobilon-P membranes (Millipore; cat. no. IPVH85R) at 120 V for 50 min on ice in 1X tris-glycine-methanol transfer buffer (25 mM tris base ...
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bioRxiv - Neuroscience 2023Quote: ... Protein gels were stained overnight with colloidal Coomassie Brilliant Blue (Sigma). Each lane was excised into 9 sections that were destained and in-gel digested overnight using trypsin (sequencing grade ...
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bioRxiv - Biochemistry 2023Quote: ... the proteins were transferred to Immobilon-P transfer membranes (Sigma-Aldrich) using the Trans-Blot® TurboTM Transfer System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were extracted as whole cell lysate using trichloroacetic acid (Sigma) and glass beads as described before87.
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bioRxiv - Developmental Biology 2022Quote: Proteins were transferred to 0.45 μm Immobilon-P PVDF membrane (Millipore) in transfer buffer (25 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... For conditional induction of protein expression 1μg/ml of doxycycline (Sigma) was added to the culture media and incubated for 16-24h.
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bioRxiv - Cell Biology 2022Quote: ... Transfer of protein on activated PVDF membranes (EMD-Millipore, cat#IPVH07850) were performed overnight at 4°C at 50mA ...
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bioRxiv - Cell Biology 2022Quote: ... Protein quantification was performed by the bicinchoninic acid method (Sigma-Aldrich) for normalization of lipolysis data.
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bioRxiv - Cancer Biology 2023Quote: Protein extracts were prepared by lysing cells in RIPA buffer (Sigma) supplemented with a phosSTOP tablet (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2023Quote: ... The protein was concentrated using an Amicon centrifugal concentrator (EMD Millipore) as necessary ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein supernatants were collected and quantified by Bradford assay (Sigma Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Resolved proteins were transferred overnight into a PVDF membrane (0.22µm, Millipore) followed by 30 min incubation in blocking buffer (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to a polyvinylidene fluoride membrane (Merck Millipore), followed by blocking in 2% BSA containing 50 mM Tris-HCl ...