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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Protachykinin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Bound proteins were eluted from magnetic beads with 1 mg/ml of 3×FLAG peptide (Sigma F4799) with agitation at 4 °C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 1 h and eluted with 0.1 mg/mL 3X FLAG peptide (Sigma-Aldrich). E-buffer (20 mM Na·HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... Following primary antibodies were used for IHC and IF: mouse anti-2A peptide (Millipore, MABS2005, 1:200), rabbit anti-Active YAP1 (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... All samples were then incubated 30min at room temperature in blocking buffer (PBS + 1% BSA, A2153-10G, Sigma) followed with primary antibodies incubation in blocking buffer overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Sections where incubated overnight in blocking solution containing the primary antibodies (Goat anti-ChAT 1:100, Millipore, AB144P). Sections were rinsed in PBS then incubated for 2 hours at room temperature in blocking solution containing secondary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... pH 9.0) followed by blocking for 1 hour at room temperature with 20v/v% goat serum (Sigma-Aldrich) in PBS ...
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bioRxiv - Biophysics 2022Quote: ... for 30 min and blocked for at least 2 h with a blocking buffer containing 1% BSA (Sigma), 3% donkey serum (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: ... Free thiol groups were blocked with blocking solution (250 mM HEPES, pH 7.7, 1 mM EDTA, 0.1 mM neocuproine [Sigma, P9599] ...
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bioRxiv - Neuroscience 2021Quote: The brain slices were incubated in blocking solution containing 1% (w/v) bovine serum albumin (Sigma, Cat#A9418), 5% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... After treating with 0.2% Triton and blocking with 1% bovine serum albumin (BSA, Sigma-Aldrich, St. Louis, USA), 3ug/ml anti-BrdU primary antibody was added O/N ...
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bioRxiv - Bioengineering 2022Quote: ... After blocking for 1 h by adding a drop of 3 % bovine serum albumin (A9418, Sigma-Aldrich, USA) onto exposed tissue ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with TSA blocking reagent containing peroxidase-conjugated sheep anti-DIG antibody (1:1,000, Sigma, 11207733910) at room temperature for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... incubated with secondary antibody diluted in blocking solution for 1 hour and counterstained with 100ng/ml DAPI (Sigma) in 1x PBS for 4 minutes before mounting on slides in Vectashield (Vector labs) ...
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bioRxiv - Neuroscience 2021Quote: Slides were brought to room temperature and incubated in blocking solution containing 1% Bovine Serum Albumin (BSA, Sigma), 0.25% Triton X-100 (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated at 4°C in blocking solution with Hoechst 33342 nuclear dye (Sigma, H21492, 1:2000) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... including an additional labeling against acetylated-α-tubuline (monoclonal, 1:1000 in blocking solution; Sigma cat No. T6793), which was detected with Alexa488 conjugated goat anti-mouse (1:250 in blocking solution ...
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bioRxiv - Developmental Biology 2020Quote: ... in 20% Goat serum, 1% Blocking reagent in TBST (5mM Tris-HCl pH 7.5, 15mM NaCl, 0.1% Triton X-100 (Sigma)) ...
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bioRxiv - Microbiology 2020Quote: ... After transfer, the membrane was incubated for 1 hour in blocking buffer (PBS, 0.1% Tween 20 (Sigma, 93773), supplemented with 5% milk or 5% BSA according to antibody recommendation) ...
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bioRxiv - Microbiology 2019Quote: ... followed by incubation with secondary antibody diluted 1/5000 in blocking solution (goat anti-mouse HRP conjugate, SIGMA) for 2 h at RT ...
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bioRxiv - Molecular Biology 2022Quote: ... 1:1000 in blocking buffer) and 0.5–0.8 μg/mL DAPI (4,6-diamidino-2-phenylindole, Sigma-Aldrich, D9542) for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Samples were subsequently incubated with primary antibodies (anti-vinculin mouse monoclonal, Sigma-Aldrich, 1:200 in blocking buffer) for 1 h at room temperature ...
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bioRxiv - Physiology 2022Quote: ... and then incubated in blocking buffer with ERα antibody (Millipore Sigma, Burlington, MA, cat#06-935, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was incubated in blocking solution (5 % normal horse serum in 1× Phosphate Buffered Saline (PBS, Sigma-Aldrich) and Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Sections were then blocked for 1 hr in blocking solution (DPBS containing 10% goat serum (Sigma Aldrich, G9023), and 0.3% Triton X-100) ...
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bioRxiv - Immunology 2024Quote: ... After cell permeabilization and blocking with 0.1% Triton-X and 1% bovine serum albumin (BSA, Sigma, catalog # 5217), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Following blocking for 30 min in PBT (PBS with 0.05% Triton X-100, Sigma Aldrich, 9002-93-1) containing 5% normal goat serum (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... The sections were treated with blocking buffer (TEM-BB) composed of 1% fish skin gelatin (Sigma-Aldrich / Merck) dissolved in 0.1M HEPES for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then rinsed in PBSDT and incubated in blocking solution 1× sheep serum (SS, Sigma Cat# S2263) for at least 4 hrs at RT ...
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bioRxiv - Biophysics 2021Quote: ... The reaction solutions contained freshly prepared 10 μM disaggregated peptide solutions (individual Aβ42 or Aβ42-clusterin peptide solutions) and 25 μM ThT (Sigma©) in dH2O ...
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bioRxiv - Immunology 2021Quote: ... with the exception that the samples were spiked with isotope-labelled peptides and MSRT retention time peptides standards (Sigma), prior to MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... coverslips were first incubated with Tris-glycine solution 5min at room temperature followed by blocking of non-specific antigens with a cold-water fish blocking buffer (Sigma-Aldrich) for 30min ...
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bioRxiv - Cell Biology 2020Quote: ... cells were permeabilized for 1 min with 0.1% Triton X-100 in PBS and saturated with blocking buffer (1% BSA in PBS) before incubation for one hour with anti-α-Tubulin (T5168. SIGMA; 1:2.000) in PBS containing 1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were blocked in 1:1 mixture of 1XPBS : Intercept PBS blocking buffer (LiCor) and then incubated with anti-PAP (Sigma, P1291, lot 92557) or anti-MYC (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... The wells were subsequently blocked for 1 hr in blocking solution (25 µL of 1% BSA [Sigma], 0.2% Tween-20 in 1x PBS) then incubated with 25 µL of the Click-iT EdU detection mix (prepared as per manufacturer’s instructions using Alex Fluor 455® ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were incubated overnight in primary antibody diluted in blocking buffer (1:1000 anti-cleaved caspase-3 Cell Signaling Technologies #9664, 1:4000 anti-α-Tubulin Sigma #T5168 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated for 72-hours in the same blocking solution with rabbit anti-CRF (Peninsula,T-4037, 1:1K) and mouse anti-TH (Millipore, MAB318, 1:5K) at 4°C on a shaker followed by 24-hours at room temperature (RT) ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Labelled SCG were sliced into 100μm sections and incubated in blocking solution for 2 hours at room temperature [Blocking solution: 10% normal donkey serum (Millipore, Billerica, MA) and 0.3% Tween 20 (Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 3x PBS washes for 5 minutes at RT before blocking with blocking buffer (BB; 5% normal donkey serum Sigma-Aldrich #S30-M, 3% BSA (Sigma #A4503) and 0.25% Triton-X100 (Sigma #T9284 ...
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bioRxiv - Bioengineering 2023Quote: ... Precusor nCys- mEpoR peptide was labeled at its free amino N-terminus as a protected peptide on resin with NHS-Biotin (Sigma) by swelling the resin with DMF having 10 equivalents N,N- Diisopropylethylamine ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminal peptide enrichment was performed on the digested sample by removing the internal tryptic peptides with undecanal (Sigma, U2202). Enriched N-terminal peptides were then desalted with a C18 spin column (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... using a subcutaneous injection of 200mg of MOG peptide (Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35 to 55; from Charité) emulsified in complete Freund’s adjuvant (from Sigma-Aldrich) that contained 200mg of Mycobacterium tuberculosis H37RA (from Difco) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with blocking solution containing 10% normal donkey serum (DNS) and 1% Triton X-100 (Sigma-Aldrich) in PBS with 0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... plates were blocked with 200 μL/well blocking buffer containing 1% (w/v) Bovine Serum Albumin (BSA, Sigma Aldrich) in PBS for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated for 1-3h in blocking solution (10% v/v Sigma-Aldrich sheep serum in PBS-T) at 21°C ...
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bioRxiv - Genomics 2019Quote: ... the blocking solution was aspirated and primary antibodies were added (mouse anti-GFP antibodies [Sigma] at 1:100 dilution). After incubation for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... BBMV were centrifuged and resuspended in binding buffer (20 mM Tris, 150 mM NaCl, 1 mM MnCl2, pH 7.4, 0,04% Blocking reagent from Sigma Aldrich). Competition binding experiments were conducted by incubating 1.4 μg of BBMV protein with 0.65 nM 125I-Vip3Aa in a final volume of 0.1 ml of binding buffer for 90 min at 25°C in the presence of increasing amounts of unlabeled Vip3Aa ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated in blocking reagent and then with FLAG M2 antibody (1:100 dilution, Sigma-Aldrich #1804) at 4 °C overnight ...