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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Protachykinin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... after which the ylmH complexes were eluted using 0.2 mg ml−1 3× Flag peptide (Sigma) in wash buffer ...
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bioRxiv - Bioengineering 2021Quote: ... 50 mL of blocking buffer was prepared by adding 1 g of biotin-free BSA (Sigma Aldrich) and 200 μL of 0.5 M pH 8.0 EDTA (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... both diluted in 1× TNB (1M Tris HCl pH 7.5, 3 M NaCl-blocking reagent, Sigma-Aldrich) with posterior incubation for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Primary antibodies were diluted in blocking buffer and incubated at room temperature for 1 hour (Sigma Aldrich M2 Flag at 1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... Blocking Buffer (100 mM HEPES, pH 7.5 [Gibco], 1 mM EDTA [Sigma-Aldrich], 2.5% SDS [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were incubated at 4°C in blocking solution with either 1:200 SOM antibody (MAB354, Millipore) or 1:5000 anti-DDDDK tag (anti-FLAG Tag to label smFP ...
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bioRxiv - Immunology 2021Quote: ... After a blocking step of 60 min at room temperature with PBS 1% BSA (Sigma-Aldrich, A3059), plates were incubated with mAb serial dilutions for 60 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... followed by 1 hour blocking in DPBS containing 10% (v/v) goat serum (Sigma-Aldrich, no. G9023). We stained each sample with 100 µl staining buffer (2% goat serum in DPBS and the indicated antibodies) ...
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bioRxiv - Neuroscience 2020Quote: ... Parasagittal sections were treated with blocking solution containing 1% normal donkey serum (Merck Millipore, Burlington, MA, USA) and 0.2% TX-100 (Nacalai Tesque ...
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bioRxiv - Physiology 2019Quote: ... They were next incubated for 30 min in Roche blocking buffer (1:10 in PBT) (#11096176001, Sigma) at RT followed by incubation with mouse anti-DIG alkaline phosphatase (AP ...
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bioRxiv - Microbiology 2021Quote: ... transferred to a PVDF membrane and blocked for at least 1h in 1% Casein blocking buffer (Sigma). Membranes were incubated overnight at 4°C with the following primary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... at 4°C in the same blocking buffer: rabbit anti-PMP22 (Sigma-Aldrich, SAB4502217, 1/750 dilution), mouse anti-β-Actin Clone AC-15 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was incubated at 4°C in blocking solution with BODIPY membrane dye (Sigma, D3821, 1:1000) for 24 hours ...
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bioRxiv - Genomics 2021Quote: ... Isolated nuclei from prefrontal cortex were resuspended in 1.5mL blocking buffer (1× PBS, 0.5% BSA (Sigma A4503), Roche Complete Protease Inhibitor without EDTA ...
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bioRxiv - Immunology 2021Quote: ... Wells were blocked for 2 hours at RT with blocking solution (PBS with 1% chicken ovalbumin; Sigma). For ELISA inhibition assay ...
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bioRxiv - Microbiology 2022Quote: ... were used at dilution 1:20000 in blocking solution and anti-mouse IgG-HRP (A-9044, Sigma) at dilution 1:80000 were used for secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were then incubated with blocking buffer (1× TBS-T, 5% goat serum (Sigma-Aldrich, G9023-5ML), 0.1 mg ml–1 salmon sperm DNA (Sigma-Aldrich ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... diluted to 1:1000 in blocking solution or rabbit anti-tyrosine hydroxylase (TH; Millipore #AB152, Burlington, MA) diluted to 1:1000 in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies diluted in blocking solution were used: rabbit-anti-Sox9 (Millipore #AB5535, 1:300), rabbit-anti-Eomes (Abcam #ab23345 ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was then blocked for at least 1 hour in 1x casein blocking buffer (Sigma–Aldrich). To detect His-tagged proteins ...
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bioRxiv - Microbiology 2023Quote: ... The sections were blocked by covering the tissue in blocking buffer (1:10 goat serum (Sigma, USA) in Tris-buffered Saline (TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... After blocking the membrane with 1% BSA in TBS and 0.1% Tween (Sigma-Aldrich, St. Louis, Missouri) (TBS-T ...
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bioRxiv - Bioengineering 2023Quote: ... and rinsed with 1×PBS before blocking and permeabilizing with 2% bovine serum albumin (BSA, Sigma-Aldrich) in 0.2% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... The blocking solution was replaced with a solution containing 1:200 anti-acetylated tubulin antibody (Sigma T6743) diluted in 10% NGS and the samples were incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... which were first incubated for 1h at room temperature in a blocking solution containing 1% BSA (Sigma) and 0.25% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were diluted in blocking solution (rat anti-HA clone 3F10, 1:1000; mouse anti-α-tubulin clone DM1A, 1:1000, both from Sigma-Aldrich). Anti-rat Alexa594 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated with primary antibodies in blocking buffer in a humidified chamber for 1 h: anti-FLAG M2 (1:2000 dilution, Sigma Aldrich, F3165) and an endogenous compartment marker antibody from rabbit (Supplementary Table 21 for list of antibodies used) ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed and then blocked for 1 h at 37 °C with blocking buffer containing 1% BSA (Sigma-Aldrich, Taufkirchen, Germany) and 0.15M sucrose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibody was diluted 1:100 in blocking buffer (rabbit-α-LAMP1: 1:100; rabbit-α-mTOR (HPA071227, Sigma-Aldrich Co. LLC): 1:12.5 ...
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bioRxiv - Microbiology 2023Quote: ... overnight at 4 °C following incubation with mouse anti-rAgApyrase antibody (1:10,000 in blocking buffer) or rabbit anti-tPA antibodies (1:2500) (Millipore-Sigma, Marlborough, MA, USA). Membranes were incubated with anti-mouse or anti-rabbit horseradish peroxidase (1:2500 in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with 5% BSA in 0.1% TBS-Tween for 1 hr at room temperature for blocking step and incubated with 1:1000 anti-RFC1 antibody produced in rabbit (Sigma-Aldrich; AV44167) at +4°C overnight ...
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bioRxiv - Microbiology 2024Quote: ... and mouse anti-ferret totIg (mAb 8H9 + mAb 4B10) diluted each to 1 µg/mL in blocking buffer (PBS containing 2% BSA [Sigma-Aldrich], 1% bovine gelatin [Sigma-Aldrich] ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by two hours blocking of unspecific binding sites using blocking buffer composed of 10% normal goat serum (NGS, Sigma), 1% bovine serum albumin (BSA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... #216763)/1X PBS for 15 minutes at 4 °C, followed by blocking with a blocking buffer (0.1 M Tris, pH 7.5, 100 mM maleic acid (Sigma-Aldrich, #M0375), 150 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... After elution with 3XFlag peptide (Sigma-Aldrich), the fusions were reverse transcribed with super script II (ThermoFisher) ...
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bioRxiv - Developmental Biology 2020Quote: ... using 100 μg/ml 3xFLAG peptide (Sigma) in lysis buffer for FLAG immunoprecipitates and 10 mM desthiobiotin in lysis buffer for Strep-Tactin immunoprecipitates ...
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bioRxiv - Molecular Biology 2021Quote: ... Digested peptides were passed over ZipTips (Millipore) and eluted with 0.5% formic acid/ 80% acetonitrile ...
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bioRxiv - Biophysics 2020Quote: ... 100 μg/mL 3×FLAG peptide (Sigma)) ...
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bioRxiv - Biochemistry 2019Quote: Peptides were desalted using C18 ZipTips (Millipore). LC-ESI-MS/MS analysis was performed using a Prominence nanoLC system (Shimadzu ...
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bioRxiv - Biochemistry 2021Quote: Peptides were desalted with C18 ZipTips (Millipore) and measured by LC-ESI-MS/MS using a Prominence nanoLC system (Shimadzu ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mg/ml 3xFlag peptide (Sigma-Aldrich) for 1h ...
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bioRxiv - Biophysics 2021Quote: ... ABCG2 was eluted with Flag peptide (Sigma) and applied to a Superdex 200 increase 10/300 column (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were desalted using C18 ZipTip (Millipore) and subjected to reverse-phase LC separation on a 60-min gradient and analyzed on a Q Exactive HF-X (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... The OVA-derived peptide SIINFEKL (S795, Sigma) was used as the positive control (400 nM) ...
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bioRxiv - Immunology 2020Quote: ... a CMV peptide pool and PHA (Sigma) were included as controls ...
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bioRxiv - Microbiology 2021Quote: ... and DRG peptides were synthesized by Sigma.
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bioRxiv - Synthetic Biology 2022Quote: All peptides were purchased from Sigma-Aldrich and used without further purification (poly-L-lysine hydrobromide (4–15 kDa ...
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bioRxiv - Biochemistry 2019Quote: Peptides were desalted with C18 ZipTips (Millipore) and measured by LC-ESI-MS/MS using a Prominence nanoLC system (Shimadzu ...
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bioRxiv - Immunology 2019Quote: ... Peptides were dissolved in DMSO (Sigma-Aldrich) to obtain a stock concentration of 3.75 μg/μl.