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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Protachykinin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... followed by a blocking and permeabilization step in 1% bovine serum albumin (Sigma, A9647-50G) in PBST (0.2% Triton X-100 in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... pH=8) and incubated for 1 h in a Western Blocking Reagent solution (Sigma-Aldrich). Membranes were incubated overnight at 4 °C with 20 ml of the respective primary antibody solution ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with rabbit anti-GAD67 (1:1000, dissolved in blocking solution; Merck Millipore SAB4300642) primary antibody for 16 h overnight ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Immunology 2022Quote: ... Secondary antibody [goat anti-human IgG alkaline phosphatase (1:10000 in blocking solution; Millipore-Sigma). A solution of p-nitrophenylphosphate (Millipore-Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were also stained with 150 µL/well FLAG (Sigma, 1:500 in blocking buffer) primary antibody and 488 (FLAG ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 min and blocking was performed by 3% bovine serum albumin (#A7906, Sigma-Aldrich) for 10 min at RT ...
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bioRxiv - Genetics 2023Quote: ... cells were blocked in blocking buffer (1% w/v bovine serum albumin (Sigma-Aldrich, A9647), 0.1% v/v Tween-20 in DPBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently blocked with blocking buffer [PBST + 1% bovine serum albumin (BSA; Millipore Sigma) + 3% donkey serum (Jackson ImmunoResearch)] at room temperature for 15 mins ...
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bioRxiv - Biochemistry 2023Quote: ... The blocking agent 6-Mercapto-1-hexanol and Phosphate-Buffered Saline (PBS) (Sigma-Aldrich Canada) (10.14 mM Na2PO4 ...
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bioRxiv - Biochemistry 2024Quote: ... The membranes were blocked for 1 hour in blocking buffer [3% Skim milk (Sigma-Aldrich) in T-TBS buffer (0.05% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 18 h after cell transfer recipient mice were immunized into the right hind paw with 50 μl of a 1:1 emulsion of 1 mg/ml MOG35-55 peptide (M4939, Sigma Aldrich) in distilled water and complete Freund’s adjuvant (incomplete Freund’s adjuvant (Sigma ...
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bioRxiv - Zoology 2021Quote: C-peptide and glucocorticoid levels were determined using a radioimmunoassay (Human C-Peptide, Merck Millipore, Billerica ...
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bioRxiv - Cell Biology 2019Quote: ... Permeabilization and blocking of samples was performed in blocking solution (10% FCS, 0.3% Saponin (47036, Sigma-Aldrich) in PBS 1x ...
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bioRxiv - Cell Biology 2021Quote: Permeabilisation and blocking were undertaken by incubating cells in ‘blocking buffer’ consisting in 3% BSA (Sigma-10735108001) & 0.5% Triton X-100 (Sigma-X100 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... blocking them in blocking buffer (5% goat serum in PBT prepared with 0.1% Tween-20, Sigma Aldrich) for an hour at room temperature followed by overnight primary antibody incubation at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight at 4 °C with primary antibodies diluted in blocking buffer (anti-vGluT1, 1:1000, guinea pig, Millipore and anti-MAP2, 1:1000, rabbit, Millipore). Sections were washed 3 times for 10 mins each in 1x PBS ...
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bioRxiv - Microbiology 2019Quote: ... the plate was washed and incubated with Streptavidin-Peroxidase diluted in blocking buffer (Sigma-Aldrich, 1 mg/mL, 1:5000) during 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Single-nucleotide EdU-labelled slides were incubated in blocking solution (1% BSA in PBS) for 30 mins and then 1:100 anti-centrin antibody (clone 20H5, Millipore) in blocking solution for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated secondary antibodies in the blocking buffer for 1 h at room temperature (1:3,000 rabbit anti-goat, Sigma-Aldrich #A5420 or 1:3,000 goat anti-mouse ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 7.5ml Intercept PBS blocking buffer with 0.1% Tween (SynGAP1; 1:1000dil, Invitrogen, PA1-046, Gapdh; 1:15000, Sigma-Aldrich G8795). Membranes were incubated with the primary antibody solution overnight at 4[ ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Fixed cultures were cut off the membrane and washed in 1 × PBS for 10 min, incubated in blocking solution (500 µl, 1 × PBS, 0,3 % TritonX (Sigma-Aldrich), 5 % goat serum ...
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bioRxiv - Biophysics 2020Quote: ... The protein was eluted from the column with Wash Buffer supplemented with 3 mg mL-1 1D4 peptide and concentrated to 4.3 mg mL-1 using a 100-kDa concentrator (Millipore) before further purifying by size exclusion chromatography using a Superose 6 increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-PaIAP (rabbit, dilution 1:2500, peptide: [H]-KAENQKARFSDVHGC-[OH], Sigma-Genosys, Haverhill, United Kingdom), anti-PaMIC10 (rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... Peptides were dissolved in 80% ACN/5%TFA with 1 M glycolic acid (Sigma-Aldrich) and incubated with 0.6 mg titanium dioxide (TiO2 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal antibodies to anti-calcitonin gene-related peptide (CGRP; 1:500; C7113, Sigma Aldrich), rat monoclonal antibodies to substance P (SP ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.4 containing 3x Flag peptide in final concentration 150 μg ml-1 (Sigma-Aldrich) for 1.5 hours at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer used was 1% bovine serum albumin (Sigma-Aldrich, St. Louis MO, A7906-50G) (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were probed with rabbit anti-detyrosinated tubulin antibody (AB3201, Sigma; 1:1000 in blocking buffer) for 1h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated in 1% Western blocking reagent (Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for 30 min and blocked for 1 h with blocking solution (Merck-Millipore, Burlington, MS, USA) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Blocking with 10% normal donkey serum was followed by ERMN primary antibody (1:200, Sigma HPA038295) overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... cochlear samples were first incubated by blocking solution containing 1% Triton X-100 (X100-500ML, Sigma) and 5% Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... at 1:400 in blocking buffer (5% FBS and 0.2% Triton X-100 [Sigma] in PBS) overnight at 4ºC ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with primary antibody rabbit anti-FOXJ1 (1:50 in blocking buffer) (Sigma-Aldrich, HPA005714) overnight at 4 ºC ...
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bioRxiv - Developmental Biology 2019Quote: ... Ovaries were then incubated in a blocking solution (PBTA: PBT supplemented with 1% BSA – Sigma A3059) for a minimum of 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... and incubated with primary antibodies diluted in blocking solution (anti-SST 1:100 v/v, Millipore, and anti-red fluorescent protein 1:400 v/v ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were then incubated with blocking buffer containing 1% bovine serum albumin (BSA, Sigma-Aldrich) for 30 min at room temperature and incubated with antibody directed against CD13 (#ab33489 ...
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bioRxiv - Neuroscience 2022Quote: Cryostat sections were incubated in “blocking solution” containing 1% (w/v) bovine serum albumin (Sigma, #A9418), 5% (v/v ...
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bioRxiv - Immunology 2020Quote: ... cells were washed with 100 μl/well of blocking buffer containing 1% (w/v) BSA (Sigma), 1 mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilization was performed for 1 hour in blocking solution containing 0.2% Triton X-100 (Sigma-Aldrich) and 10% donkey serum (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2022Quote: ... Permeabilization and blocking was performed by incubation in PBS with 1% (m/v) BSA (Sigma Aldrich), 0.2% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated for 1 h with a blocking solution containing 3% BSA (BP9703-100; Sigma-Aldrich). Cells were incubated for 1 h with anti-ACBD3 and anti-GM130 primary antibodies followed by 1 h incubation with appropriate Alexa Fluor Dyes ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by incubation with blocking solution (1% BSA, 3% Donkey Serum [Sigma: D9663], 0.1% (v/v) Tween in PBS ...
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bioRxiv - Bioengineering 2024Quote: ... Blocking and permeabilization steps were done with 1% BSA and 0.5 % cold fish gelatin (Sigma-Aldrich) dissolved in Tris + 0.1% Triton solution ...
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bioRxiv - Immunology 2021Quote: Synthetic peptide analogues for validation targets were analysed together with MSRT retention time peptides standards (Sigma) by LC-MS/MS using an Orbitrap Fusion Lumos mass spectrometer ...
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bioRxiv - Cancer Biology 2019Quote: ... Resultant peptides were further concentrated and 3 × FLAG peptide removed using Amicon centrifugal filtration devices (Millipore).
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bioRxiv - Neuroscience 2020Quote: ... After a 2 h blocking step in blocking buffer (PBX with 5% normal goat serum, Sigma-Aldrich, G9023) at RT ...
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bioRxiv - Biophysics 2019Quote: ... Peptide concentrations (Direct Detect spectrophotometer, Millipore) in injection buffer (5% HPLC grade acetonitrile ...