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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Protachykinin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were then washed twice with PBS prior to 20 min permeabilization with 0.5% Triton X-100 and 1 h blocking with 3% BSA (Sigma) at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was then incubated in blocking buffer for 1 hour at 25°C (TBST with 3% BSA; Sigma) before being probed for LapA or MapA containing an HA tag with an anti-HA antibody (Biolegend ...
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bioRxiv - Biophysics 2022Quote: ... permeabilization step was followed by an hour of incubation with blocking buffer comprising 1% bovine serum albumin solution (Sigma) with 0.1% tween (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated at RT for 1 hour in a blocking solution containing 3% Donkey Serum (Biowest) and 0.3% Triton X-100 (Sigma) in PBS 0.01M ...
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bioRxiv - Neuroscience 2022Quote: Brain sections were first incubated for 1h at room temperature (RT) in a blocking solution containing 1% BSA (Sigma), 2% Donkey Serum (Biowest ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were incubated with the secondary antibody (1:1000 in blocking solution) and 1µg/ml DAPI (#D9564, Sigma) for 2 hours at room temperature (RT) ...
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bioRxiv - Genetics 2021Quote: ... in PBS for 20 minutes at 4°C followed by a 1 hour room temperature incubation in blocking solution of 5% donkey serum (Biosera) in 0.3% Triton-X-100 (Sigma) in PBS (0.3% PBST) ...
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bioRxiv - Cancer Biology 2021Quote: ... washed again with PBS followed by a blocking step for 1 hr with PBS + 0.1% Tween-20 (Sigma-Aldrich), 2% BSA (PAA Laboratories GmbH ...
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bioRxiv - Cancer Biology 2022Quote: ... for 30 minutes at room temperature and non-specific blocking with 1% bovine serum albumin (BSA; A2153, Sigma-Aldrich) and 10% normal goat serum (NGS ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibody in blocking solution (1:500 mouse anti-gamma tubulin (Sigma Aldrich, GTU-88), 1:3000 mouse anti-ARL13B (Biolegend ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated in blocking solution (1% heat-inactivated horse serum in 0.1% Triton-X/PBS; Millipore Sigma) either 1 hour at 37°C or overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized in acetone at −20 °C for 10 minutes and then incubated for two hours in blocking solution (10 mg/ml BSA, 1% DMSO, 0.5% Triton X-100 (Sigma) and 4% normal goat serum in 0.1M phosphate buffer or 1X PBS) ...
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bioRxiv - Immunology 2021Quote: ... Slides were washed 5 times with PBS and blocked with blocking buffer (PBS + 1% bovine serum albumin (BSA, Sigma, cat no ...
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bioRxiv - Developmental Biology 2019Quote: ... the samples were incubated with the blocking buffer (0.1% Triton X-100, 5% normal donkey serum (NDS, Stratech) and 1% bovine serum albumin (BSA, Sigma)) for 30 minutes at RT ...
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bioRxiv - Immunology 2021Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were blocked in Odyssey blocking buffer TBS (Licor) or 5% non-fat milk or 1% BSA (Sigma, A7906) for 1 hour before over-night incubation at 4°C with primary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were then incubated in a blocking solution of 1% bovine serum albumin (BSA; Sigma-Aldrich, A8022, Lot SLBT9577) and 22.52 mg/mL UltraPure Glycine (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma)) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in blocking solution containing goat anti-choline acetyltransferase (ChAT, 1:250, Millipore Cat# AB144P, RRID:AB_2079751) and rabbit anti- KV2.1 (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Cell pellets were then resuspended in 1ml of permeabilization/blocking buffer (0.1% Triton X-100, 1% BSA, 10% normal goat serum (Sigma) in PBS) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 50mL of blocking buffer was prepared per 2 × 108 cells by adding 1 g biotin-free BSA (Sigma Aldrich) and 200 mL of 0.5 M pH 8.0 EDTA into DPBS (GIBCO) ...
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bioRxiv - Developmental Biology 2023Quote: ... incubated overnight at 4°C in blocking buffer with primary antibody (1:500 mouse anti-Elastin, Millipore Sigma MAB2503), washed ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody was applied overnight at 4°C in blocking buffer (α-Actinin, Sigma A7811, 1:2500; AURKB, Sigma A5102 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were spun down and resuspended in blocking solution with 1:500 anti-FLAG M2 monoclonal antibody (Sigma [F1804]) and incubated over night at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... FLAG and AgRP primary antibodies were used and diluted in blocking buffer at 1:200 (mouse anti-FLAG, Sigma) and 1:1000 (rabbit anti-AgRP ...
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bioRxiv - Cell Biology 2021Quote: ... Primary and secondary antibody incubations were performed for 1 hour at 25°C in blocking buffer with anti-Flag-M1 (Sigma-Aldrich, #F3040, 1:1000) conjugated with Alexa-647 (Molecular Probes ...
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bioRxiv - Neuroscience 2022Quote: ... secondary antibodies were applied for 30 min at RT at 1:400 dilution in blocking solution containing 1:10,000 4’,6-diamidino-2-phenylindole (DAPI; Sigma Aldrich, St. Louise, MO, USA) in the closed humid chamber ...
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bioRxiv - Immunology 2020Quote: ... in 50 μL of blocking buffer were added for 1 hour followed by 50 μL ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5000 dilution) in blocking buffer for 1 hour at room temperature (RT) ...
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bioRxiv - Neuroscience 2022Quote: ... and then blocked for 1 hour in blocking buffer (1% BSA and 10% normal donkey serum, 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA). Primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... the calcium free medium was replaced with the cell culture medium with or without E-cadherin blocking monoclonal antibody DECMA-1 (Millipore, 10 µg mL−1), and cells migration was carried out for 4 hours at 37°C in a 5% CO2 humidified incubator.
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in primary antibodies (diluted in the blocking solution, see Table 1) against myc (rabbit c-Myc, 1:1000, Sigma-Aldrich, C3956, RRID: AB_439680) and FLAG (mouse-FLAG ...
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bioRxiv - Cell Biology 2022Quote: ... Nitrocellulose membranes were blocked for 1 h with the blocking buffer containing 5 % dried milk in TBS-Tween 1 % and incubated over-night with primary antibodies: anti-αTubulin (Sigma, T6074, 1:1000 dilution), anti-acetylated Tubulin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... in 50 μl of blocking buffer were added for 1 h followed by 50 μl ExtrAvidin-Alkaline phosphatase (E2636, Sigma-Aldrich, 1:5.000 dilution) in blocking buffer for 1 h at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated in blocking buffer for 1 hr at room temperature followed by incubation with rabbit anti-RBM10 (Sigma-Aldrich HPA034972, 1:2000), rabbit anti-HDAC1 (GeneTex GTX100513 ...
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bioRxiv - Cell Biology 2024Quote: ... Permeabilization buffer was removed and washed 3×2 minutes before blocking for 1 hour at room temperature in a 1% Bovine Serum Albumin (BSA) (Millipore Sigma, cat: 2910-OP) in DPBS solution ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then incubated overnight at 4°C with primary antibodies in blocking solution: rabbit anti-GFP (MBL Cat# 598; 1/300) and mouse anti-HA (Sigma, Cat# 12ca5; 1/50). On the next day ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Plant Biology 2022Quote: The coding sequence of CHLI without transit peptide (1-46 amino acid residues) was cloned into pET32a (Novagen) and pGEX-4T-1 (GE healthcare ...
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bioRxiv - Biochemistry 2019Quote: ... The pure recombinant protein was eluted from the column using the same buffer with 0.1 mg.mL-1 FLAG peptide (Sigma). Pure PRMT7 was flash frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM TCEP) before elution using wash buffer supplemented with 0.25 mg/mL 3xFLAG peptide (F4799, Sigma-Aldrich). The eluent was then subjected to another round of affinity purification using anti-HA resin (26181 ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... and then incubated with 3 resin volumes of 200 µg·ml-1 3xFLAG peptide (Sigma, catalog # F4799, lot # SLCJ4916) for 45 min to elute proteasome complexes ...
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bioRxiv - Biochemistry 2022Quote: ... GB1-TAZ2 binding to MITF peptides was determined utilising a polyhistidine monoclonal mouse antibody (1:5000, Sigma, H1029), incubated on the array for 4 hours at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT) before elution using wash buffer supplemented with 0.25 mg/ml 3xFLAG peptide (F4799, Sigma-Aldrich). Purified telomerase complex was verified by western blotting.
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bioRxiv - Biophysics 2023Quote: ... The Aβ (1–42) peptide (Stratech, A-1163, 0.5 mg) was initially dissolved in 100% HFIP (Sigma-Aldrich) at a concentration of 4 μM ...
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bioRxiv - Cell Biology 2020Quote: ... ICC blocking solution (3% w/v BSA (Sigma-Aldrich), 3% v/v goat serum (Abcam) ...
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bioRxiv - Neuroscience 2021Quote: ... blocking with 5% (w/v) BSA (#A9418, Sigma-Aldrich) and staining for MAP2 only ...
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bioRxiv - Immunology 2019Quote: ... followed by blocking with 5% skim milk (Sigma-Aldrich) and incubation with indicated primary antibodies ...