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Citations for Millipore Sigma :
401 - 450 of 5781 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Cancer Biology 2023Quote: ... We also assessed cell proliferation by using Cell Counting Kit-8 (CCK-8) assay (Sigma, France) according to the instruction ...
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bioRxiv - Systems Biology 2024Quote: ... 8-Br cAMP (8-Bromoadenosine 3′,5′-cyclic monophosphate, final conc. 500 µM; Sigma B6386-100mg). In addition to stimulation for decidualization ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Physiology 2019Quote: ... Ultra Pure Water (8 µl, SIGMA) and forward and reverse primers for Lkb1 ...
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bioRxiv - Developmental Biology 2019Quote: ... 8 nM β-estradiol (Sigma, E8875), 200 ng/mL progesterone (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and 8 μg/ml polybrene (Sigma,) was added ...
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bioRxiv - Biochemistry 2020Quote: ... 8 mg/mL BSA (Millipore Sigma), and 1 mg/mL K-casein (Millipore Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... pH 8 with BSA (Sigma, A3059) and incubated ...
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bioRxiv - Neuroscience 2022Quote: ... L-(-) Glucose (Sigma –921-60-8) and D-(+)-Glucose (50-99-7) ...
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bioRxiv - Neuroscience 2022Quote: ... and 8□μM leupeptin (Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2022Quote: ... 8 nM β-estradiol (Sigma, E2758), 200 ng/mL progesterone (Selleck ...
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bioRxiv - Cell Biology 2022Quote: ... 8 mM KCl (Sigma, catalog #: P9541), 6 mM MgCl2 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... and CHIR99021 (8 μM, Sigma-Aldrich). On day 2 ...
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bioRxiv - Pathology 2022Quote: ... Ultra Pure Water (8 μl, SIGMA) and fwd and rev primers (QuantiTect ...
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bioRxiv - Biophysics 2019Quote: ... and 8 μM CHIR99021 (Sigma-Aldrich) for 48 h ...
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bioRxiv - Synthetic Biology 2019Quote: 1.00e-8 moles fluorescein (Sigma-Aldrich).
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bioRxiv - Biochemistry 2019Quote: ... MWCO 6-8 kDa (Millipore Sigma). The 23 kDa A3B-CTD was verified using Coomassie Blue staining after PAGE (data not shown) ...
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bioRxiv - Neuroscience 2019Quote: ... ICI 118,55113 (Sigma, 72795-01-8): 10 mg kg−1 ...
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bioRxiv - Cell Biology 2021Quote: ... 8 μg/ml pepstatin A (Sigma) and 4 mM DTT (Sigma) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 M Urea (Sigma Aldrich, 51457), and Guanidine Hydrochloride (Serva ...
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bioRxiv - Cell Biology 2020Quote: ... A mix of 8% acrylamide (Sigma) and 0.264% bis-acrylamide solution (Sigma ...
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bioRxiv - Immunology 2021Quote: ... 8 mM DBCO-amine (Sigma-Aldrich) in 150 mM NaCl ...
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bioRxiv - Microbiology 2021Quote: ... 8% Fetal bovine serum (Sigma Aldrich), 5% HEPES buffer (Gibco ...
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bioRxiv - Immunology 2020Quote: ... ATM inhibitor (Sigma, #925701-46-8), doxycycline (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... glucose (8 mg/ml, Sigma-Aldrich), penicillin (200 U/ml)–streptomycin (200 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... 8 mM of NAD (Sigma-Aldrich) or TMG cap was added ...
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bioRxiv - Genomics 2022Quote: ... 8% dextran sulfate sodium salt (Sigma), and 2× SSC ...
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bioRxiv - Neuroscience 2022Quote: ... Glucose (8 mg/ml, Sigma Aldrich), Penicillin (200 U/mL)-Streptomycin (200μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... and 10-8 M dexamethasone (Sigma). To prevent the bone pieces from moving ...
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bioRxiv - Biochemistry 2022Quote: ... 8% formaldehyde (#F8775-25ML, Sigma-Aldrich) prediluted in 1X PBS were added to the tube ...