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Citations for Millipore Sigma :
351 - 400 of 5781 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 8 μg/mL polybrene (Millipore) and 10 mM HEPES (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... 8 μg/ml polybrene (Sigma) was added.
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bioRxiv - Cancer Biology 2022Quote: ... clone ASC-8 (Sigma, #MAB2059) and 1 μg mL−1 E-cadherin antibody ...
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bioRxiv - Molecular Biology 2022Quote: ... 8% Ficoll PM70 (Sigma F2878), 0.05% NP-40 (Sigma) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 0.5mM 8-Br-cAMP (Sigma), and 1μM of the progesterone analog medroxyprogesterone acetate (Sigma ...
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bioRxiv - Physiology 2022Quote: ... 8% gelatin (G1890, Sigma-Aldrich), and 1% polyvinylpyrrolidone (P5288 ...
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bioRxiv - Cell Biology 2023Quote: ... 8-trimethylpsoralen (2mg/ml, Sigma) for 5 min in the dark and then exposed to 365 nm UV light for 8 min in a UV Stratalinker 1800 ...
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bioRxiv - Microbiology 2023Quote: ... 8 mM Mg-ATP (Sigma), and 2.5 mM Bz-Leu-Leu43 (Laboratory of Dr ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then uniformly coated with 10% cupric sulfate (Sigma 451657)/8% phosphoric acid (Sigma P6550) solution ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2% 8-hydroxyquinoline (Sigma) was added to each lysate (1:1 v/v phenol:lysate ...
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bioRxiv - Biophysics 2023Quote: ... 8 g/mol ATP (Sigma), 4 mM theophylline) ...
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bioRxiv - Bioengineering 2023Quote: ... CCK-8 solution (Sigma-Aldrich) was pre-warmed for 5 mins at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... 10-8 M dexamethasone (Sigma), 50 µg/ml L-Ascorbate Phosphate (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... MWCO 6-8 kDa (Millipore). BRD4Tandem (residues 43-459 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8 (S9640, Sigma-Aldrich) for 2 hours at 4°C with end-over-end rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8 (S9640, Sigma-Aldrich) for 2 hours at 4°C with end-over-end rotation ...
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bioRxiv - Physiology 2023Quote: ... 8% gelatine (G1890, Sigma-Aldrich) and 1% polyvinylpyrrolidone (PVP ...
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bioRxiv - Physiology 2023Quote: ... on fluid secretion rate, 10-4 M 8 bromo-cAMP (cAMP) (23, 66) and 10-8 M 8 bromo-cGMP (cGMP) (4) (Sigma-Aldrich, Oakville, ON, Canada) were used against unstimulated MTs ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Bioengineering 2019Quote: ... and 0.5 mM 8-Bromoadenosine 3’,5’-cyclic monophosphate (8-Br-cAMP; Sigma-Aldrich B5386) in EGM for 6 days [30–34] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 0.5 mM 8-Bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP; B5386, Sigma Aldrich). After 48 hrs ...
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bioRxiv - Molecular Biology 2021Quote: Cell viability was assessed using CCK-8 assay (Cell Counting Kit-8, Sigma-Aldrich, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... a cell proliferation and viability reagent (Cell Counting kit-8, cck-8 assay, Sigma Aldrich) was utilized following the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... IL-4 (20 ng/mL, Sigma, 11276905001) or PBS ...
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bioRxiv - Biophysics 2019Quote: ... and 50 U/ml IL-2 (Sigma) followed by spin-infection for 90 minutes at 30°C and 1000g ...
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bioRxiv - Immunology 2022Quote: ... and 10U/mL IL-2 (11147528001, Sigma).
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bioRxiv - Neuroscience 2023Quote: ... IL-1α (3 ng/mL, Sigma I3901) and TNF (30 ng/mL ...
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bioRxiv - Microbiology 2023Quote: ... 5 ng/ml IL-4 (Sigma I1020), and 20 µg/ml LPS (Sigma L-2630) ...
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bioRxiv - Genetics 2023Quote: ... and IL-6 (#RAB0309, Sigma Aldrich, GmbH) following manufacturer’s recommendations ...
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bioRxiv - Biophysics 2024Quote: IL-2 (Sigma Aldrich Cat #: I0523-5X20UG) was added to a final concentration of 1.25 µg/mL on Day 2 ...
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bioRxiv - Immunology 2024Quote: ... and IL-13 10ng/ml (Sigma-Aldrich) were isolated ...
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bioRxiv - Immunology 2020Quote: Cell lines (HEK293T, HEK293, 3C11, 143B, MDCK-SIAT1, MDCK-PR8) were maintained in DMEM (Sigma Aldrich) supplemented with 10% FCS (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells overexpressing Flag-AurkB was treated with 10 µM of trichostatin A (TSA) (Sigma-Aldrich) or DMSO for 12 hours ...
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bioRxiv - Bioengineering 2021Quote: ... for HEK293-T and SW480 cells were coated with fibronectin bovine plasma (F1141-2MG; Sigma Aldrich) before seeding cells onto the 8-well plates ...
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bioRxiv - Genomics 2021Quote: ... HEK293 and NSC were transfected with STARR-seq plasmid containing enhancer regions using polyethylenimine (PEI, Sigma) or Lipofectamine™ Stem Transfection Reagent (Thermo Scientific ...
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YAP promotes cell-autonomous immune responses to tackle intracellular Staphylococcus aureus in vitrobioRxiv - Cell Biology 2022Quote: HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Protein production was done in HEK293-6E cells by transient transfection with polyethylenimine (PEI, Sigma-Aldrich) and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: 2×105 HEK293 were seeded onto 24well plates previously pre-coated with poly-L-lysine (Sigma), and infected with 2×105 ...
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bioRxiv - Immunology 2020Quote: ... this mixture was applied to mammalian FreeStyle™ HEK293-F cell culture (EMD-Millipore, Darmstadt, Germany) at a density of 1 million cells per milliliter ...
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bioRxiv - Neuroscience 2023Quote: ... and mock plasmids were transfected into HEK293 suspension cells derived from tsA201 (Millipore Sigma, 96121229-1VL) using Polyethylenimine (Polysciences ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...