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Citations for Millipore Sigma :
201 - 250 of 6152 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: GFP+/- LKS and GMPs were sorted as described above and live cells were imaged in 8 chamber borosilicate coverglass system (nunc) coated with human plasma fibronectin (EMD Millipore) using a Leica TCS SP8 confocal microscope equipped with two photomultiplier tubes ...
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bioRxiv - Immunology 2023Quote: ... 100,000 T84 cells per well were seeded on glass bottom 8-well chamber slides coated with 2.5% human collagen (Sigma #C5533-5MG) diluted in water ...
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bioRxiv - Cancer Biology 2022Quote: ... or Cell Counting Kit-8 (CCK-8) assay (Sigma Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: Cell Counting Kit 8 (CCK-8) assay (Sigma Aldrich - 96992) was used to assess cell viability according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... WST-8 reagent (cell counting kit 8, Sigma-Aldrich, 96992) was added to each well (10 μl/well ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5μl of Cell Counting Kit – 8 (CCK-8, Sigma, UK) was added to each well and cells were then incubated for another 2 hrs at 37°C in 5% CO2 and in the dark ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... pH:8 (Sigma) and precipitated with Isopropanol the following day ...
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bioRxiv - Molecular Biology 2020Quote: ... pH:8 (Sigma) and then precipitated with equal volume of isopropanol in presence of glycogen ...
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bioRxiv - Molecular Biology 2020Quote: ... 8-trimethylpsoralen (Sigma, T6137 ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected using Polyethylenimine (PEI,Sigma Aldrich; St. Louis, Missouri, USA). PEI and vector media were combined in a ratio of 5µL PEI and 0.5µg vector in 250µL of serum-free DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... HEK293 cells were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Genetics 2019Quote: ... amodiaquin dihydrochloride and 8-hydroxyquinoline quinoline (8-HQ) (all from Sigma), were introduced into separate wells ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 5 μL of Cell Counting Kit – 8 (CCK-8, Sigma, UK) was added to each well and the cells were incubated for an additional 2-3 hours at 37 °C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... 4 μL of Cell Counting Kit-8 (CCK-8; Sigma-Aldrich) was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... 8-methoxypsoralen (8-MOP) was from Sigma-Aldrich (St Louis, MO). 8-MOP and AMT were dissolved in DMSO and stored as aliquots at −80°C prior to use.
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bioRxiv - Neuroscience 2023Quote: ... or 3-octanol (Sigma, 8 µl in 8 ml mineral oil) were used as olfactory stimuli ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...