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Citations for Millipore Sigma :
601 - 650 of 5781 citations for IL 8 CXCL8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Neuroscience 2021Quote: ... with 64 channels per well in 8×8 electrode grids were coated with filter sterilized 0.1% poly(ethylenimine) solution (Sigma) in borate buffer pH 8.4 for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... data was separated into pre-defined frequency bins (Delta 0.5-4 Hz, Theta 4-8 Hz, Alpha 8-13 Hz, Sigma 12-15 Hz ...
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bioRxiv - Bioengineering 2023Quote: Cell viability of various materials were determined using CCK-8 assay (Sigma Cell Counting Kit-8, BCCB8166, Sigma Aldrich). SH-SY5Y cells were seeded in 96-well plates at a density of 5,000 cells per well ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 CV of wash buffer 2 (40 mM Tris pH 8, 300 mM NaCl, 20 mM imidazole pH 8, 2.15% sodium cholate (Sigma), 1% Triton X-100) ...
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bioRxiv - Cell Biology 2023Quote: Cell viability assay was performed using the cell proliferation reagent Cell Counting Kit-8 (CCK-8) following the manufacturer’s protocol (Sigma). Briefly ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2020Quote: ... Lung tumors were induced in 6-8 weeks old mice by intraperitoneal injection of 8 doses of 1 g/kg of urethane (ethyl carbamate; Sigma); second dose was given 48h after the initial one and then once a week to reach a total of 8 doses ...
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bioRxiv - Cancer Biology 2019Quote: ... ADORA1 selective and competitive antagonists 1-Butyl-3-(3-hydroxypropyl)-8-(3-noradamantyl) xanthine (PSB36) and 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) (Sigma-Aldrich); ADORA2b selective antagonist 4-(2,3,6,7-Tetrahydro-2 ...
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bioRxiv - Systems Biology 2020Quote: ... paired SUSD2- and SUSD2+ cells were seeded 6-well plates at a density of 2×105 cells/well and decidualized when 90% confluent with 0.5 mM 8-bromoadenosine cAMP (8-bromo-cAMP; Sigma-Aldrich) and 1 µM medroxyprogesterone acetate (MPA ...
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bioRxiv - Biophysics 2020Quote: Pre-purified FIPV nsp7+8 and HCoV-229E nsp7+8 at 20 μM were cross-linked with 0.15 % glutaraldehyde (Sigma-Aldrich) at 4 °C for 25 min before diluting them to 1 μM in MALDI matrix solution (sinapinic acid 10 mg/mL in acetonitrile/water/TFA ...
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bioRxiv - Biophysics 2020Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Genomics 2021Quote: ... per time point were infected with the lentivirus library with a multiplicity of infection (MOI) of 5-8 along with 8 μg/mL polybrene (Sigma). Three independent replicate cultures were infected ...
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bioRxiv - Neuroscience 2021Quote: ... partial 5-HT2A/2CR agonist), and 8-hydroxy-2-(di-n-propylamino)tetraline (8-OH-DPAT, 5-HT1A agonist) were obtained from Sigma/Aldrich ...
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bioRxiv - Physiology 2022Quote: The membrane-permeable 8-Br-cGMP (8-bromoguanosine 3′,5′-cyclic monophosphate, B1381) and 8-Br-cAMP (8-bromoadenosine 3′,5′-cyclic monophosphate, B5386) were from Sigma. ANP (AS-20648) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with lentiviral solution for 8-12 h in the presence of 8 μg/mL Polybrene (Sigma- Aldrich).
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bioRxiv - Developmental Biology 2023Quote: ... The growth medium of organoids was supplemented with 100μM with 8-Bromoadenosine 3’,5’-cyclic monophosphate sodium salt (8-Br-cAMP) (Sigma, #B7880) in iPSC organoids or 200μM forskolin (FSK ...
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bioRxiv - Biochemistry 2024Quote: ... Cell viability under 42 h compound treatment was determined via the Cell Counting Kit-8 (CCK-8, Sigma-Aldrich #96992) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... human Akt1 (Sigma-Aldrich) or 0.3 μg active ...
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bioRxiv - Biochemistry 2022Quote: ... Purified human ApoA1 (Sigma) or purified ApoA1-ApoM protein was resuspended in PBS (10:1 mol/mol ...
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bioRxiv - Bioengineering 2019Quote: Human holo Tf (Sigma) was conjugated to AF700 or AF750 (Life Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... human (GluFib, Sigma Aldrich) was added (0.3 ng/µl ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
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bioRxiv - Neuroscience 2021Quote: ... human fibronectin (Sigma-Aldrich) 5 µg/ml ...